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EW172 CMV-TO-UTRN-14ZF-VP64 (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    cgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgMluISpeICMV20406080100120140160
    gccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccNdeICMV180200220240260280300320340
    cagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacagtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgtNcoICspCISnaBICMV360380400420440460480500520
    actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtBsmBIEsp3ISgrDICMVTetR binding sitesTetOTetO540560580600620640660680700
    tagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgCACGAGTGTGCCCGATGTGGGAAGAATTTCTCTTGGCACTCAGACCTCATCTTGCACGAGCAAATCCACACAGGAGAGCGACCTTTCAAGTGTTACAAGTGTGGACGTGGatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacGTGCTCACACGGGCTACACCCTTCTTAAAGAGAACCGTGAGTCTGGAGTAGAACGTGCTCGTTTAGGTGTGTCCTCTCGCTGGAAAGTTCACAATGTTCACACCTGCACCBbsIPmeIXmnIAflIIBmgBIHindIIIMH2EC4AR6CG8KN10FS12WH14SD16LI18LH20EQ22IH24TG26ER28PF30KC32YK34CG36RG38763-1623MH2EC4AR6CG8KN10FS12WH14SD16LI18LH20EQ22IH24TG26ER28PF30KC32YK34CG36RG38763-1431M1UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q...21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)720740760780800820840860
    ATTCGCAGAGCACGGGACACTCAATCGACACCTTAGAACTAAGGGCGGATGTACTGGGGAGCGGCCTTACAATTGTGAGGAGTGTGGAAGAGCTTTCTCCCAAGCTAGCCATCTCCAAGACCACCAAAGGCTTCACACTGGGGAGAGACCCCACCAATGTATCGAGTGTGGAAAGTAAGCGTCTCGTGCCCTGTGAGTTAGCTGTGGAATCTTGATTCCCGCCTACATGACCCCTCGCCGGAATGTTAACACTCCTCACACCTTCTCGAAAGAGGGTTCGATCGGTAGAGGTTCTGGTGGTTTCCGAAGTGTGACCCCTCTCTGGGGTGGTTACATAGCTCACACCTTTCBaeIBmtIMfeINheISapIBseRIBspQIFA40EH42GT44LN46RH48LR50TK52GG54CT56GE58RP60YN62CE64EC66GR68AF70SQ72AS74HL76QD78HQ80RL82HT84GE86RP88HQ90CI92EC94GK96763-1623FA40EH42GT44LN46RH48LR50TK52GG54CT56GE58RP60YN62CE64EC66GR68AF70SQ72AS74HL76QD78HQ80RL82HT84GE86RP88HQ90CI92EC94GK96763-1431UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)8809009209409609801,0001,0201,040
    TCCTTCAATAGACACTGTAATCTTATAAGGCACCAAAAGATACACACGGGTGAGCGGCCATTCGAGTGTTCTAAGTGTGGGAAGGCTTGTACTAGAAGGTGTAATTTGATACAACACCAAAAGGTCCACACTGGTGAGCGTCCTTTCGCTTGTAGGTTCTGTGCTAAGCCTTTCAAGGAAGTTATCTGTGACATTAGAATATTCCGTGGTTTTCTATGTGTGCCCACTCGCCGGTAAGCTCACAAGATTCACACCCTTCCGAACATGATCTTCCACATTAAACTATGTTGTGGTTTTCCAGGTGTGACCACTCGCAGGAAAGCGAACATCCAAGACACGATTCGGAAAGTBlpIPsiISF98NR100HC102NL104IR106HQ108KI110HT112GE114RP116FE118CS120KC122GK124AC126TR128RC130NL132IQ134HQ136KV138HT140GE142RP144FA146CR148FC150AK152PF154763-1623SF98NR100HC102NL104IR106HQ108KI110HT112GE114RP116FE118CS120KC122GK124AC126TR128RC130NL132IQ134HQ136KV138HT140GE142RP144FA146CR148FC150AK152PF154763-1431UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GACGTTCTTCAGACATGCGAGACCACGAGAGAGTCCACACTGGGGAGAGACCATTCGCATGTCGGTTCTGTGCCAAGCCTTTCAGACGATCTTCCGACATGCGGGACCACGAGAGGGTTCACACAGGGGAGCGTCCCTTCCAATGTGAGCTCTGTTCATACACTTGTCCCAGAAGCTGCAAGAAGTCTGTACGCTCTGGTGCTCTCTCAGGTGTGACCCCTCTCTGGTAAGCGTACAGCCAAGACACGGTTCGGAAAGTCTGCTAGAAGGCTGTACGCCCTGGTGCTCTCCCAAGTGTGTCCCCTCGCAGGGAAGGTTACACTCGAGACAAGTATGTGAACAGGGTCTTCRR156SS158DM160RD162HE164RV166HT168GE170RP172FA174CR176FC178AK180PF182RR184SS186DM188RD190HE192RV194HT196GE198RP200FQ202CE204LC206SY208TC210PR212R763-1623RR156SS158DM160RD162HE164RV166HT168GE170RP172FA174CR176FC178AK180PF182RR184SS186DM188RD190HE192RV194HT196GE198RP200FQ202CE204LC206SY208TC210PR212R763-1431UTRN Promoter Pos(-14)-Binding ZF (Q5JNZ3 finger 1-Q66K89 finger 9-Q9NYT6 finger 19-O15535 finger 5-Q13398 finger 21-Q8NCA9 finger 4-Q8NCA9 finger 4-P49711 finger 1)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GTCAAATTTAGACAGACACATGAAGTCCCACgctgctgcaggcggaagcggaggctctggcggatctgatgccctggacgacttcgacctggatatgctgggcagcgacgccctggatgattttgatctggacatgctgggatctgacgctctggacgatttcgatctcgacatgCAGTTTAAATCTGTCTGTGTACTTCAGGGTGcgacgacgtccgccttcgcctccgagaccgcctagactacgggacctgctgaagctggacctatacgacccgtcgctgcgggacctactaaaactagacctgtacgaccctagactgcgagacctgctaaagctagagctgtacPciIPstIA1A2A3G4G5S6G7G8S9G10G11S12D13A14L15D16D17F18D19L20D21M22L23G24S25D26A27L28D29D30F31D32L33D34M35L36G37S38D39A40L41D42D43F44D45L46D47M481432-1623S214NL216DR218HM220KS222HA224AA226GG228SG230GS232GG234SD236AL238DD240FD242LD244ML246GS248DA250LD252DF254DL256DM258LG260SD262AL264DD266FD268LD270M763-1623S214NL216DR218HM220KS222H763-1431VP64UTRN Promote...1 finger 1)1,4201,4401,4601,4801,5001,5201,5401,560
    ttgggatcagatgcactggatgactttgacctggacatgctcggatcataggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttaaccctagtctacgtgacctactgaaactggacctgtacgagcctagtatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaaApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIEcoO109IL49G50S51D52A53L54D55D56F57D58L59D60M61L62G63S641432-1623L272GS274DA276LD278DF280DL282DM284LG286S763-1623*1VP64c-myc epitopeBGH poly...equencePolyhi...e tag1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttBGH polyadenylation sequence1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcacBbsIClaISphIBspDIPvuIIBGH pABGH po...uence1,9401,9601,9802,0002,0202,0402,0602,0802,100
    accgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgNaeINgoMIV2,1202,1402,1602,1802,2002,2202,2402,260
    gtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaaBarIMscIXbaIBsaXIBsmBIEsp3IPflFIPshAITth111IHygromycinFRT2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccaEcoRIDraIIIHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgcagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgacNcoINdeIPstIPvuIAsiSIBfuAIBspMIRsrIIBstAPIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggttaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccaPflFIBstAPIDraIIINmeAIIITth111IHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    cattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgacgtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactgAjuISacIIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    caactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggScaIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttatctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataNaeIPfoINgoMIVHygromycinSV...A3,3403,3603,3803,4003,4203,4403,4603,4803,500
    tgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggaBsmIPsiIBstZ17ISV40 pA3,5203,5403,5603,5803,6003,6203,6403,660
    gtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcPvuII3,6803,7003,7203,7403,7603,7803,8003,8203,840
    cggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaagcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttPciISapIBsaXIBspQIpUC ori3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcapUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    ccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttAlwNIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtacBspHIpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcAhdIAmp (bla)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacBsrDINmeAIIIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    ccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggAclIFspIBsrDIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaaBcgIPvuIScaIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcAclIXmnIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatSspIBspHIbla promoterAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    ggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaatAlwNISgrDI5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatatcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatMfeIBsiWIBpu10I5,9605,9806,0006,0206,040
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    6040 bp45005000550050010001500VP64TetR binding sitesTetO763-1431763-16231432-1623763-765EW112.1 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)MluI,SpeI,NdeICMV,SnaBI,NcoI,CspCITATA box,CMV-Forward Genewiz (DNA)TetO,SgrDI,BsmBI,Esp3IKozak,start codon,CMVF1 (DNA),ToFlpF (DNA),EKbF (DNA),+5XmnI,BmgBIMfeIEWo231.1 (DNA),EWo229.3 (DNA),BspQI,SapI,BseRI,NheI,BmtI,+1PsiI+1EWo233.1 (DNA)BlpIEWo231.4 (DNA)EWo100.5 (DNA),PstIEW112.2 (DNA)BspHIAhdIBsrDINmeAIIIFspI,AclI,BsrDIAmp (bla)PvuIScaI,BcgIAclI,XmnIbla promoter,SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),SgrDI,AlwNI,MfeI,Bpu10I,BsiWI

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    6040