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pSB1A3_BBa_K4720104
  • Sequence Map
    Editing disabled on read-only sequence.
    AATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCampR20406080100120140160
    TTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCBcgIPvuIScaITatIampR180200220240260280300320340
    TTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATATAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTATATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAApaLIampR360380400420440460480500520
    TTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATBsrBIampR540560580600620640660680700
    AACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTcgctaaggatgatttctggAATTCGCGGCCGCTTCTAGATTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGTCTTAAAGTCTATTTTTTTTAGGAATCGAAAgcgattcctactaaagaccTTAAGCGCCGGCGAAGATCTEagINotIXbaIZraIAatIIEcoRIVF2termina...m loopprefix720740760780800820840860
    GttgacggctagctcagtcctaggtacagtgctaGCTACTAGAGAAAGAGGAGAAATACTAGATGTGGCGTGAAACGAAGCTGCTGTTAATCGATGATAACCTGGACCGTTCTCGTGATTTAGCGGTGATATTGAATTTCTTAGGAGAGGACCAATTAACTTGTAATAGTGAAGACaactgccgatcgagtcaggatccatgtcacgatCGATGATCTCTTTCTCCTCTTTATGATCTACACCGCACTTTGCTTCGACGACAATTAGCTACTATTGGACCTGGCAAGAGCACTAAATCGCCACTATAACTTAAAGAATCCTCTCCTGGTTAATTGAACATTATCACTTCTBmtIBmtINheINheIAvrIIFleQ_Codon_OptimizedBBa_J23100RBS...018809009209409609801,0001,0201,040
    CTGGAGAGAAGTTGCTGCTGGGCTCTCTAATTCTCGTGAGGCTCTCTGTGTTTTACTGGGATCCGTTGAATCTAAAGGTGGCGCAGTTGAACTCCTCAAACAGCTCGCTTCCTGGGATGAATATTTACCAATTTTGTTAATTGGTGAACCAGCGCCAGCTGATTGGCCTGAGGAAGACCTCTCTTCAACGACGACCCGAGAGATTAAGAGCACTCCGAGAGACACAAAATGACCCTAGGCAACTTAGATTTCCACCGCGTCAACTTGAGGAGTTTGTCGAGCGAAGGACCCTACTTATAAATGGTTAAAACAATTAACCACTTGGTCGCGGTCGACTAACCGGACTCCTTBamHIBanIIBstXIPvuIIBsu36IFleQ_Codon_Optimized1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTGCGTCGACGAGTTCTTGCATCTTTGGAAATGCCGCCGTCCTATAATAAATTACTGGACTCTTTACACCGTGCTCAAGTTTATCGTGAAATGTATGATCAAGCACGTGAACGGGGACGCAGTCGTGAACCAAATTTATTTAGATCGCTCGTAGGTACGAGTCGCGCCATACAACAACGCAGCTGCTCAAGAACGTAGAAACCTTTACGGCGGCAGGATATTATTTAATGACCTGAGAAATGTGGCACGAGTTCAAATAGCACTTTACATACTAGTTCGTGCACTTGCCCCTGCGTCAGCACTTGGTTTAAATAAATCTAGCGAGCATCCATGCTCAGCGCGGTATGTTGPmlISalIPflFISgrDITth111IFleQ_Codon_Optimized1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AAGTTCGACAAATGATGCAACAAGTAGCGGACACTGACGCTTCAGTTTTGATTCTGGGTGAAAGCGGGACAGGGAAAGAGGTAGTCGCCCGAAATTTACACTATCACAGCAAACGTCGTGAAGGACCATTTGTTCCTGTTAATTGTGGTGCCATTCCAGCCGAGCTTTTAGAGTCTTCAAGCTGTTTACTACGTTGTTCATCGCCTGTGACTGCGAAGTCAAAACTAAGACCCACTTTCGCCCTGTCCCTTTCTCCATCAGCGGGCTTTAAATGTGATAGTGTCGTTTGCAGCACTTCCTGGTAAACAAGGACAATTAACACCACGGTAAGGTCGGCTCGAAAATCTCAGAloIBanIFleQ_Codon_Optimized1,4201,4401,4601,4801,5001,5201,5401,560
    GGAGCTGTTTGGTCATGAAAAGGGCGCATTTACAGGGGCGATTACTAGCCGAGCTGGGCGCTTTGAACTTGCTAATGGTGGTACGTTGTTTCTTGATGAAATAGGAGATATGCCCCTTCCCATGCAAGTAAAACTCTTACGTGTTTTACAAGAACGTACGTTTGAGCGCGTAGGTCCTCGACAAACCAGTACTTTTCCCGCGTAAATGTCCCCGCTAATGATCGGCTCGACCCGCGAAACTTGAACGATTACCACCATGCAACAAAGAACTACTTTATCCTCTATACGGGGAAGGGTACGTTCATTTTGAGAATGCACAAAATGTTCTTGCATGCAAACTCGCGCATCCABsiWIFleQ_Codon_Optimized1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGTAATAAAACCCAAAATGTAGATGTTCGTATAATAGCGGCTACGCATAAGAATCTGGAAAAGATGATAGAAGACGGGACCTTTCGTGAGGATTTATATTATCGTCTGAATGTGTTTCCTATCGAAATGGCCCCTTTGCGTGAACGAGTGGAAGATATAGCATTATTACTTAATGTCATTATTTTGGGTTTTACATCTACAAGCATATTATCGCCGATGCGTATTCTTAGACCTTTTCTACTATCTTCTGCCCTGGAAAGCACTCCTAAATATAATAGCAGACTTACACAAAGGATAGCTTTACCGGGGAAACGCACTTGCTCACCTTCTATATCGTAATAATGAATTACPpuMIEcoO109IFleQ_Codon_Optimized1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AGCTTATTAGTCGTATGGAACACGAAAAGCGGGGAAGTATTCGTTTTAATAGCGCCGCTATTATGTCACTGTGTCGTCATGATTGGCCAGGAAATGTTAGAGAGCTTGCTAATCTCGTTGAAAGACTTGCAATAATGCACCCGTATGGCGTTATAGGTGTTGGGGAGTTACCTAATCGAATAATCAGCATACCTTGTGCTTTTCGCCCCTTCATAAGCAAAATTATCGCGGCGATAATACAGTGACACAGCAGTACTAACCGGTCCTTTACAATCTCTCGAACGATTAGAGCAACTTTCTGAACGTTATTACGTGGGCATACCGCAATATCCACAACCCCTCAATGGATTMscIFleQ_Codon_Optimized1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAAGTTTCGACACGTGGATGATGAAGATGAACAGCTTGCATCTTCCTTAAGAGAGGAATTGGAAGAAAGAGCCGCAATTAATGCAGGCCTTCCCGGTATGGATGCTCCCGCTATGCTTCCCGCAGAGGGACTTGATTTAAAAGATTATTTGGCTAATTTAGAACAAGGGTTGATTCTTCAAAGCTGTGCACCTACTACTTCTACTTGTCGAACGTAGAAGGAATTCTCTCCTTAACCTTCTTTCTCGGCGTTAATTACGTCCGGAAGGGCCATACCTACGAGGGCGATACGAAGGGCGTCTCCCTGAACTAAATTTTCTAATAAACCGATTAAATCTTGTTCCCAACTAAPmlIStuIAflIIBstAPIFleQ_Codon_Optimized2,1202,1402,1602,1802,2002,2202,2402,260
    CAACAAGCTCTGGATGATGCAGGCGGCGTTGTTGCCCGCGCAGCGGAGCGTCTTCGTATACGGCGGACTACATTAGTTGAAAAGATGCGTAAATATGGTATGAGTCGGCGTGACGACGACCTGTCGGATGATTGATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGTTGTTCGAGACCTACTACGTCCGCCGCAACAACGGGCGCGTCGCCTCGCAGAAGCATATGCCGCCTGATGTAATCAACTTTTCTACGCATTTATACCATACTCAGCCGCACTGCTGCTGGACAGCCTACTAACTATGATCTCGGTCCGTAGTTTATTTTGCTTTCCGAGTCAGCAhdIBstZ17IFleQ_Codon_Optimizeddouble terminator 2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGATAATGGTTGCTACTAGAGCGCCTAAAAATTGTTTCTGACCCGGAAAGCAAAATAGACAACAAACAGCCACTTGCGAGAGATGATCTCAGTGTGACCGAGTGGAAGCCCACCCGGAAAGACGCAAATATATGATCTCATTGTGGCACGCACAACTGATAAAATGGAGACCGCCACTATTACCAACGATGATCTCGCGGATTTTTAACPsrIdouble terminator Pcl lamR0051Ppe...012,4602,4802,5002,5202,5402,5602,5802,6002,620
    ACAGTTTCCGCTTAAAAATTTAGCAATTAGCATATTTAGTCATTAGATTGACGTTAATCGCCTGCGGCTGCTTTGCCCGTGCCCGCACTGCCAGGGATTCGACGTTGCCACCGGAAACCGCATCCATGCTTCCGTACTAGAGAAAGAGGAGAAATActagatgactaaaaaaattTGTCAAAGGCGAATTTTTAAATCGTTAATCGTATAAATCAGTAATCTAACTGCAATTAGCGGACGCCGACGAAACGGGCACGGGCGTGACGGTCCCTAAGCTGCAACGGTGGCCTTTGGCGTAGGTACGAAGGCATGATCTCTTTCTCCTCTTTATgatctactgatttttttaaMT2KK4ILuxCPpel K4242001RBS...FP2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcattcattattaacggccaggttgaaatctttcccgaaggtgatgatttagtgcaatccattaattttggtgataatagtgtttacctgccaatattgaatgactctcatgtaaaaaacattattgattgtaatggaaataacgaattacggttgcataacattgtcaattttcagtaagtaataattgccggtccaactttagaaagggcttccactactaaatcacgttaggtaattaaaaccactattatcacaaatggacggttataacttactgagagtacattttttgtaataactaacattacctttattgcttaatgccaacgtattgtaacagttaaaagS6FI8IN10GQ12VE14IF16PE18GD20DL22VQ24SI26NF28GD30NS32VY34LP36IL38ND40SH42VK44NI46ID48CN50GN52NE54LR56LH58NI60VN62FLuxC2,8202,8402,8602,8802,9002,9202,9402,960
    tctatacggtagggcaaagatggaaaaatgaagaatactcaagacgcaggacatacattcgtgacttaaaaaaatatatgggatattcagaagaaatggctaagctagaggccaattggatatctatgattttatgttctaaaggcggcctttatgatgttgtagaaaatgaactagatatgccatcccgtttctacctttttacttcttatgagttctgcgtcctgtatgtaagcactgaatttttttatataccctataagtcttctttaccgattcgatctccggttaacctatagatactaaaatacaagatttccgccggaaatactacaacatcttttacttgaAjuIBlpIEcoRVL64YT66VG68QR70WK72NE74EY76SR78RR80TY82IR84DL86KK88YM90GY92SE94EM96AK98LE100AN102WI104SM106IL108CS110KG112GL114YD116VV118EN120EL122LuxC2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tggttctcgccatatcatggatgaatggctacctcaggatgaaagttatgttcgggcttttccgaaaggtaaatctgtacatctgttggcaggtaatgttccattatctgggatcatgtctatattacgcgcaattttaactaagaatcagtgtattataaaaacatcgtcaaccaccaagagcggtatagtacctacttaccgatggagtcctactttcaatacaagcccgaaaaggctttccatttagacatgtagacaaccgtccattacaaggtaatagaccctagtacagatataatgcgcgttaaaattgattcttagtcacataatatttttgtagcagttggTatIBsrGIBsu36IGS124RH126IM128DE130WL132PQ134DE136SY138VR140AF142PK144GK146SV148HL150LA152GN154VP156LS158GI160MS162IL164RA166IL168TK170NQ172CI174IK176TS178ST180LuxC3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gatccttttaccgctaatgcattagcgttaagttttattgatgtagaccctaatcatccgataacgcgctctttatctgttatatattggccccaccaaggtgatacatcactcgcaaaagaaattatgcaacatgcggatgttattgtcgcttggggagggccagatgcgattactaggaaaatggcgattacgtaatcgcaattcaaaataactacatctgggattagtaggctattgcgcgagaaatagacaatatataaccggggtggttccactatgtagtgagcgttttctttaatacgttgtacgcctacaataacagcgaacccctcccggtctacgctaatAleIDP182FT184AN186AL188AL190SF192ID194VD196PN198HP200IT202RS204LS206VI208YW210PH212QG214DT216SL218AK220EI222MQ224HA226DV228IV230AW232GG234PD236AI238LuxC3,3403,3603,3803,4003,4203,4403,4603,4803,500
    attgggcggtagagcatgcgccatcttatgctgatgtgattaaatttggttctaaaaagagtctttgcattatcgataatcctgttgatttgacgtccgcagcgacaggtgcggctcatgatgtttgtttttacgatcagcgagcttgtttttctgcccaaaacatatattacattaacccgccatctcgtacgcggtagaatacgactacactaatttaaaccaagatttttctcagaaacgtaatagctattaggacaactaaactgcaggcgtcgctgtccacgccgagtactacaaacaaaaatgctagtcgctcgaacaaaaagacgggttttgtatataatgtaSphIZraIAatIINW240AV242EH244AP246SY248AD250VI252KF254GS256KK258SL260CI262ID264NP266VD268LT270SA272AT274GA276AH278DV280CF282YD284QR286AC288FS290AQ292NI294YY296MLuxC3,5203,5403,5603,5803,6003,6203,6403,660
    gggaaatcattatgaggaatttaagttagcgttgatagaaaaacttaatctatatgcgcatatattaccgaatgccaaaaaagattttgatgaaaaggcggcctattctttagttcaaaaagaaagcttgtttgctggattaaaagtagaggtggatattcatcaacgttggatgccctttagtaatactccttaaattcaatcgcaactatctttttgaattagatatacgcgtatataatggcttacggttttttctaaaactacttttccgccggataagaaatcaagtttttctttcgaacaaacgacctaattttcatctccacctataagtagttgcaacctacFspAIG298NH300YE302EF304KL306AL308IE310KL312NL314YA316HI318LP320NA322KK324DF326DE328KA330AY332SL334VQ336KE338SL340FA342GL344KV346EV348DI350HQ352RW354MLuxC3,6803,7003,7203,7403,7603,7803,8003,8203,840
    attattgagtcaaatgcaggtgtggaatttaatcaaccacttggcagatgtgtgtaccttcatcacgtcgataatattgagcaaatattgccttatgttcaaaaaaataagacgcaaaccatatctatttttccttgggagtcatcatttaaatatcgagatgcgttagcattaataataactcagtttacgtccacaccttaaattagttggtgaaccgtctacacacatggaagtagtgcagctattataactcgtttataacggaatacaagtttttttattctgcgtttggtatagataaaaaggaaccctcagtagtaaatttatagctctacgcaatcgtaattSwaIBmgBIPaqCIBstAPII356IE358SN360AG362VE364FN366QP368LG370RC372VY374LH376HV378DN380IE382QI384LP386YV388QK390NK392TQ394TI396SI398FP400WE402SS404FK406YR408DA410LA412LLuxC3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaggtgcggaaaggattgtagaagcaggaatgaataacatatttcgagttggtggatctcatgacggaatgagaccgttgcaacgattagtgacatatatttctcatgaaaggccatctaactatacggctaaggatgttgcggttgaaatagaacagactcgattcctggaagattccacgcctttcctaacatcttcgtccttacttattgtataaagctcaaccacctagagtactgccttactctggcaacgttgctaatcactgtatataaagagtactttccggtagattgatatgccgattcctacaacgccaactttatcttgtctgagctaaggaccttctBsaIPfoIK414GA416ER418IV420EA422GM424NN426IF428RV430GG432SH434DG436MR438PL440QR442LV444TY446IS448HE450RP452SN454YT456AK458DV460AV462EI464EQ466TR468FL470EE472LuxC4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agataagttccttgtatttgtcccataataggtaaaaagtatggaaaatgaatcaaaatataaaaccatcgaccacgttatttgtgttgaaggaaataaaaaaattcatgtttgggaaacgctgccagaagaaaacagcccaaagagaaagaatgccattattattgcgtctggttctattcaaggaacataaacagggtattatccatttttcataccttttacttagttttatattttggtagctggtgcaataaacacaacttcctttatttttttaagtacaaaccctttgcgacggtcttcttttgtcgggtttctctttcttacggtaataataacgcagaccaCspCIME2NE4SK6YK8TI10DH12VI14CV16EG18NK20KI22HV24WE26TL28PE30EN32SP34KR36KN38AI40II42AS44GLuxDDK474FL476VF478VP480*LuxC4,2204,2404,2604,2804,3004,3204,3404,360
    tttgcccgcaggatggatcattttgctggtctggcggaatatttatcgcggaatggatttcatgtgatccgctatgattcgcttcaccacgttggattgagttcagggacaattgatgaatttacaatgtctataggaaagcagagcttgttagcagtggttgattggttaactaaaacgggcgtcctacctagtaaaacgaccagaccgccttataaatagcgccttacctaaagtacactaggcgatactaagcgaagtggtgcaacctaactcaagtccctgttaactacttaaatgttacagatatcctttcgtctcgaacaatcgtcaccaactaaccaattgatF46AR48RM50DH52FA54GL56AE58YL60SR62NG64FH66VI68RY70DS72LH74HV76GL78SS80GT82ID84EF86TM88SI90GK92QS94LL96AV98VD100WL102TLuxD4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cacgaaaaataaataacttcggtatgttggcttcaagcttatctgcgcggatagcttatgcaagcctatctgaaatcaatgcttcgtttttaatcaccgcagtcggtgttgttaacttaagatattctcttgaaagagctttagggtttgattatctcagtctacccattaatgagtgctttttatttattgaagccatacaaccgaagttcgaatagacgcgcctatcgaatacgttcggatagactttagttacgaagcaaaaattagtggcgtcagccacaacaattgaattctataagagaactttctcgaaatcccaaactaatagagtcagatgggtaattactBarIAflIIT104RK106IN108NF110GM112LA114SS116LS118AR120IA122YA124SL126SE128IN130AS132FL134IT136AV138GV140VN142LR144YS146LE148RA150LG152FD154YL156SL158PI160NE162LuxD4,5604,5804,6004,6204,6404,6604,6804,7004,720
    attgccgaataacctagattttgaaggccataaattgggtgctgaagtctttgcgagagattgtcttgattttggttgggaagatttagcttctacaattaataacatgatgtatcttgatataccgtttattgcttttactgcaaataacgataattgggtcaagcaagatgaataacggcttattggatctaaaacttccggtatttaacccacgacttcagaaacgctctctaacagaactaaaaccaacccttctaaatcgaagatgttaattattgtactacatagaactatatggcaaataacgaaaatgacgtttattgctattaacccagttcgttctacttLP164NN166LD168FE170GH172KL174GA176EV178FA180RD182CL184DF186GW188ED190LA192ST194IN196NM198MY200LD202IP204FI206AF208TA210NN212DN214WV216KQ218DE220LuxD4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gttatcacattgttatcaaatattcgtagtaatcgatgcaagatatattctttgttaggaagttcgcatgacttgagtgaaaatttagtggtcctgcgcaatttttatcaatcggttacgaaagccgctatcgcgatggataatgatcatctggatattgatgttgatattactgcaatagtgtaacaatagtttataagcatcattagctacgttctatataagaaacaatccttcaagcgtactgaactcacttttaaatcaccaggacgcgttaaaaatagttagccaatgctttcggcgatagcgctacctattactagtagacctataactacaactataatgacNruIBtgZIVI222TL224LS226NI228RS230NR232CK234IY236SL238LG240SS242HD244LS246EN248LV250VL252RN254FY256QS258VT260KA262AI264AM266DN268DH270LD272ID274VD276IT278LuxD4,9204,9404,9604,9805,0005,0205,0405,060
    aaccgtcatttgaacatttaactattgcgacagtcaatgaacgccgaatgagaattgagattgaaaatcaagcaatttctctgtcttaaaatctattgagatattctatcactcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccttggcagtaaacttgtaaattgataacgctgtcagttacttgcggcttactcttaactctaacttttagttcgttaaagagacagaattttagataactctataagatagtgagtttatcgttatattcctgagagatactttaaacctttgaaaaacgaatgtatggttggaggMK2FG4NF6LL8TY10QP12PLuxAEP280SF282EH284LT286IA288TV290NE292RR294MR296IE298IE300NQ302AI304SL306S*308LuxD5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggttaaaagagtttgtctccattactttgcaaaccaatttaatccagcgtagagactcctcacaccaaaactatggcataccaatgacctcgtagtaaagtgcctcaaaccaaacgaaccattgggaatacagcgacgacgtataaatgaaccgcgctgattttttaacttacatBstEIIQ14FS16QT18EV20MK22RL24VK26LG28RI30SE32EC34GF36DT38VW40LL42EH44HF46TE48FG50LL52GN54PY56VA58AA60YL62LG64AT66KK68LN70VLuxA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatccttgacggcgataacaagaagggtgtcgggtaggtcatgcggttgaacttctacacttaaataacctagtttacagttttcctgctaaagccaaaccataaacggctcccgaaatgttgttcctgaaagcgcataagccgtgtctatacttattgtcagcgcggaatcgccttaG72TA74AI76VL78PT80AH82PV84RQ86LE88DV90NL92LD94QM96SK98GR100FR102FG104IC106RG108LY110NK112DF114RV116FG118TD120MN122NS124RA126LA128ELuxA5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctcgaccatgcccgactatttcttaccgtactgtctccctatataccttcgactattacttgtatagttcaaggtattccattttcatttggggcgccgcatatcgtctccaccgcgtggccaaatacaccaccgacttagtcgaagctgctgactcacccgacgagttaaaccggaAgeIBsrFISacIIC130WY132GL134IK136NG138MT140EG142YM144EA146DN148EH150IK152FH154KV156KV158NP160AA162YS164RG166GA168PV170YV172VA174ES176AS178TT180EW182AA184QF186GL188LuxA5,6205,6405,6605,6805,7005,7205,7405,760
    accgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaattttggctactataattcaacctaatatttatgattgcttttctttcgtgttgaactcgaaatattacttcaccgagttcttatacccgtgctataagtattatagctggtaacgaatagtatatattgtagacatctagtactgagttaatttcgctttctctaaacggcctttaaaPM190IL192SW194II196NT198NE200KK202AQ204LE206LY208NE210VA212QE214YG216HD218IH220NI222DH224CL226SY228IT230SV232DH234DS236IK238AK240EI242CR244KF246LuxA5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcgaccccgtaaccatactaagaatacacttacgatgctgataaaaactactaagtctggtttgttctccaatactaaagttatttcccgtcaccgcactgaaacataattttcctgtatttctatgattagcggcataactaatgtcaatgctttagttagggcacccttgcggcgLG248HW250YD252SY254VN256AT258TI260FD262DS264DQ266TR268GY270DF272NK274GQ276WR278DF280VL282KG284HK286DT288NR290RI292DY294SY296EI298NP300VG302TP304LuxA5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaagtccttacataactgtattaagtttttctgtaactacgatgtccttatagtttataaacaacacctaaacttcgattaccttgtcatctgctttaataacgaaggtacttcgagaaggtcagactacagtacggtaaagaatttctttttgttgcaagcgataatataatcgattcAjuIBcgISapIBspQIQE306CI308DI310IQ312KD314ID316AT318GI320SN322IC324CG326FE328AN330GT332VD334EI336IA338SM340KL342FQ344SD346VM348PF350LK352EK354QR356SL358LY360*LuxA6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctcctctttctttactttaaacctaacaagaaggaattgaagtagttaagttgttgacaagttcttgtttcatatcaagcgtacgtcctttattgcctcatacaactattcaacttaaaacttgtctaaaatcacatacttttagtaaaaagtctattaccacaacagccgcgaggagSphIMK2FG4LF6FL8NF10IN12ST14TV16QE18QS20IV22RM24QE26IT28EY30VD32KL34NF36EQ38IL40VY42EN44HF46SD48NG50VV52GA54PLuxB6,3206,3406,3606,3806,4006,4206,4406,460
    tgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtccgcatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatactgacaaagaccaaaagacgagccaaattgtctcttttaattttaaccaagtaatttagtgtagtaatgttgagtagtaggacaggcgtatcgcctccttcgaacgaataacctagtcaattcacttccctctaaataaaatcccaaatcactaacgcttttttttctactttaL56TV58SG60FL62LG64LT66EK68IK70IG72SL74NH76II78TT80HH82PV84RI86AE88EA90CL92LD94QL96SE98GR100FI102LG104FS106DC108EK110KD112EM114LuxB6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtacgtaaaaaaattagcgggccaacttatagttgtcgttgataaacttctcacaatactttagtaattgctacgaaattgttgtccgataacattaggtctattgctaaaaatatcgaagggattttatagacatttaggggtacgaatatgcggtccgcctggagcctttatacatHF116FN118RP120VE122YQ124QQ126LF128EE130CY132EI134IN136DA138LT140TG142YC144NP146DN148DF150YS152FP154KI156SV158NP160HA162YT164PG166GP168RK170YV172LuxB6,6606,6806,7006,7206,7406,7606,7806,8006,820
    acagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttatgtcgttggtcagtagtataacaactcacccgccggttttttccataaggagagtagaaattcaccctactaagattactacaatctatacttatacgactttctatatttcggcaacgcctatttatactgcaactggatagtctctatctggtagtcaattactataatcaatTA174TS176HH178IV180EW182AA184KK186GI188PL190IF192KW194DD196SN198DV200RY202EY204AE206RY208KA210VA212DK214YD216VD218LS220EI222DH224QL226MI228LV230LuxB6,8406,8606,8806,9006,9206,9406,9606,9807,000
    actataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaatgatattgcttctatcattatttcgatttgttctctgcgcacgtaaataatcactaatacaagaactttacgtgggattacttttaaagcttttatttgaacttctttattaacgtcttttgcgacagcctttaatatgcctcacatattgacgccgattcaaccgttaacttttMluIBsmBIBstBIEsp3INY232NE234DS236NK238AK240QE242TR244AF246IS248DY250VL252EM254HP256NE258NF260EN262KL264EE266II268AE270NA272VG274NY276TE278CI280TA282AK284LA286IE288KLuxB7,0207,0407,0607,0807,1007,1207,1407,160
    gtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaatagatttcgagttgcagcgaggcggcaagtgaacgaatccccaggagcatagataaccacaccacgcttttcacataacgacaggaaacttggttacttactaaactactcggtttttttacattagttataacaactactattataattcttcatggtgtaccttatatggattatctaaagctcaacgtcgctccgccgttcacttgcttaggggtcctcgtatctattgC290GA292KS294VL296LS298FE300PM302ND304LM306SQ308KN310VI312NI314VD316DN318IK320KY322HM324EY326T*328LuxB7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tatgtgactggggtgagtgaaagcagccaacaaagcagcagcttgaaagatgaagggtataaaagagtatgacagcagtgctgccatactttctaatattatcttgaggagtaaaacaggtatgacttcatatgttgataaacaagaaattacagcaagctcagaaattgatgatatacactgaccccactcactttcgtcggttgtttcgtcgtcgaactttctacttcccatattttctcatactgtcgtcacgacggtatgaaagattataatagaactcctcattttgtccatactgaagtatacaactatttgttctttaatgtcgttcgagtctttaactactaNdeIMT2SY4VD6KQ8EI10TA12SS14EI16DD18LuxE7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttgattttttcgagcgatccattagtgtggtcttacgacgagcaggaaaaaatcagaaagaaacttgtgcttgatgcatttcgtaatcattataaacattgtcgagaatatcgtcactactgtcaggcacacaaagtagatgacaatattacggaaattgatgacatacctgtataactaaaaaagctcgctaggtaatcacaccagaatgctgctcgtccttttttagtctttctttgaacacgaactacgtaaagcattagtaatatttgtaacagctcttatagcagtgatgacagtccgtgtgtttcatctactgttataatgcctttaactactgtatggacataBstXILI20FS22SD24PL26VW28SY30DE32QE34KI36RK38KL40VL42DA44FR46NH48YK50HC52RE54YR56HY58CQ60AH62KV64DD66NI68TE70ID72DI74PV76LuxE7,5407,5607,5807,6007,6207,6407,6607,6807,700
    tcccaacatcggtttttaagtttactcgcttattaacttctcaggaaaacgagattgaaagttggtttaccagtagcggcacgaatggtttaaaaagtcaggtggcgcgtgacagattaagtattgagagactcttaggctctgtgagttatggcatgaaatatgttggtagttgagggttgtagccaaaaattcaaatgagcgaataattgaagagtccttttgctctaactttcaaccaaatggtcatcgccgtgcttaccaaatttttcagtccaccgcgcactgtctaattcataactctctgagaatccgagacactcaataccgtactttatacaaccatcaacFP78TS80VF82KF84TR86LL88TS90QE92NE94IE96SW98FT100SS102GT104NG106LK108SQ110VA112RD114RL116SI118ER120LL122GS124VS126YG128MK130YV132GS134WLuxE7,7207,7407,7607,7807,8007,8207,8407,860
    gtttgatcatcaaatagaattagtcaatttgggaccagatagatttaatgctcataatatttggtttaaatatgttatgagtttggtggaattgttatatcctacgacatttaccgtaacagaagaacgaatagattttgttaaaacattgaatagtcttgaacgaataaaaaatcaaactagtagtttatcttaatcagttaaaccctggtctatctaaattacgagtattataaaccaaatttatacaatactcaaaccaccttaacaatataggatgctgtaaatggcattgtcttcttgcttatctaaaacaattttgtaacttatcagaacttgcttattttttaF136DH138QI140EL142VN144LG146PD148RF150NA152HN154IW156FK158YV160MS162LV164EL166LY168PT170TF172TV174TE176ER178ID180FV182KT184LN186SL188ER190IK192NLuxE7,8807,9007,9207,9407,9607,9808,0008,0208,040
    caagggaaagatctttgtcttattggttcgccatactttatttatttactctgccattatatgaaagataaaaaaatctcattttctggagataaaagcctttatatcataaccggaggcggctggaaaagttacgaaaaagaatctctgaaacgtgatgatttcaatcatctttgttccctttctagaaacagaataaccaagcggtatgaaataaataaatgagacggtaatatactttctatttttttagagtaaaagacctctattttcggaaatatagtattggcctccgccgaccttttcaatgctttttcttagagactttgcactactaaagttagtagaaaBglIIQ194GK196DL198CL200IG202SP204YF206IY208LL210CH212YM214KD216KK218IS220FS222GD224KS226LY228II230TG232GG234WK236SY238EK240ES242LK244RD246DF248NH250LLuxE8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tatttgatactttcaatctcagtgatattagtcagatccgagatatatttaatcaagttgaactcaacacttgtttctttgaggatgaaatgcagcgtaaacatgttccgccgtgggtatatgcgcgagcgcttgatcctgaaacgttgaaacctgtacctgatggaacgccgggataaactatgaaagttagagtcactataatcagtctaggctctatataaattagttcaacttgagttgtgaacaaagaaactcctactttacgtcgcatttgtacaaggcggcacccatatacgcgctcgcgaactaggactttgcaactttggacatggactaccttgcggcccAfeIPciIL252FD254TF256NL258SD260IS262QI264RD266IF268NQ270VE272LN274TC276FF278ED280EM282QR284KH286VP288PW290VY292AR294AL296DP298ET300LK302PV304PD306GT308PG310LuxE8,2408,2608,2808,3008,3208,3408,3608,3808,400
    gttgatgagttatatggatgcgtcagcaaccagttatccagcatttattgttaccgatgatgtcgggataattagcagagaatatggtaagtatcccggcgtgctcgttgaaattttacgtcgcgtcaatacgaggacgcagaaagggtgtgctttaagcttaaccgaagcgtttcaactactcaatatacctacgcagtcgttggtcaataggtcgtaaataacaatggctactacagccctattaatcgtctcttataccattcatagggccgcacgagcaactttaaaatgcagcgcagttatgctcctgcgtctttcccacacgaaattcgaattggcttcgcaaaLM312SY314MD316AS318AT320SY322PA324FI326VT328DD330VG332II334SR336EY338GK340YP342GV344LV346EI348LR350RV352NT354RT356QK358GC360AL362SL364TE366AF368LuxE8,4208,4408,4608,4808,5008,5208,5408,560
    gatagttgaTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGctatcaactATGATCATCGCCGGCGACGTCAGGCCGTTTTTTCCCGTTCCACAGTGGTGGGACGGGAAAAAGAAATTTTGGCTTTTCTAATGAAGCGCAATACGTCCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCEagINotIPstISpeIBsrBIDS370*LuxEterminatorsuffixVR8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTGTCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGDrdIPciIori_pMB1VR8,7608,7808,8008,8208,8408,8608,8808,9008,920
    CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTori_pMB18,9408,9608,9809,0009,0209,0409,0609,0809,100
    GCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCAlwNIApaLIori_pMB19,1209,1409,1609,1809,2009,2209,2409,260
    CCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCori_pMB19,2809,3009,3209,3409,3609,3809,4009,4209,440
    ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTAC9,4609,4809,5009,5209,5409,5609,5809,6009,620
    CTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCAhdIBanIBsaIBsrFIampR9,6409,6609,6809,7009,7209,7409,7609,7809,800
    CCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAABglIampR9,8209,8409,860
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9863 bp700075008000850090009500500100015002000LuxEMutLux_Rev (DNA)MutLux_Fwd (DNA)Lux_Fwd (DNA)ampR,PvuI,TatI,ScaI,BcgI,ApaLIVF2,end of terminator,terminator stem loop,prefix,prefix,BsrBI,ZraI,AatII,+4BBa_J23100,scar 1,RBS J34801,scar 2,NheI,BmtI,AvrII,NheI,BmtI,BanII,+1PvuII,BstXI,Bsu36I,SalI,SgrDI,PmlIPflFI,Tth111IFleQ_Codon_Optimized,AloI,BanIBsiWI,EcoO109I,PpuMIMscIPmlI,AflIIBstAPI,StuIscar 3,BstZ17I,AhdIdouble terminator NdeIBstXIBglIIPciIAfeIsuffix,terminator,SpeI,EagI,NotI,PstI,BsrBIVR,PciI,DrdIori_pMB1,ApaLI,AlwNIBanI,AhdI,BsaI,BsrFI,BglI

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    9863