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pCrU6.4-SaCas9_cloning/aphVIII
  • Sequence Map
    Editing disabled on read-only sequence.
    gatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccNaeIBsaAIBtgZINgoMIVF1 ori20406080100120140160
    caagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaaggttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcFspIPvuIILacZ alphaF1 ori180200220240260280300320340
    ttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccgggctgcTCTTTGACGAGCGCTATCACGCTGTGCCATTGGTGGTCaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcacttaacattatgctgagtgatatcccgcttaacctcgaggtggcgccaccgccggcgagatcttgatcacctagggggcccgacgAGAAACTGCTCGCGATAGTGCGACACGGTAACCACCAGAfeIAleIEagINotISacISmaISpeIXbaIXmaIBamHIBstXITspMIEco53kIPU6.4T7Fragme...fwd))M13-fwd360380400420440460480500520
    CTGTTCTGGAAATCCATGGAAATCCCCTCGACGCAGGCCACCCCGACACCACAGCCAAGCGCAGGTCTGCTGGGCTCTGCGAAATCGAATGCAAGTTTAACTGGTGCGCGCGTTCCCTGGACCCTGATTGGCCCATTTTAGAACATTCATGAAGTTGATATACTGCAGATATGCGGACAAGACCTTTAGGTACCTTTAGGGGAGCTGCGTCCGGTGGGGCTGTGGTGTCGGTTCGCGTCCAGACGACCCGAGACGCTTTAGCTTACGTTCAAATTGACCACGCGCGCAAGGGACCTGGGACTAACCGGGTAAAATCTTGTAAGTACTTCAACTATATGACGTCTATACGCBarIBsmINcoIPstIStyIPU6.4540560580600620640660680700
    AGAGATAATAAACTAAGCAGTCGGAGGTCGGCCATGCCTCGTGACAGCGGCGTCAACTCCGAGTTCCTGCGGTTCCTGTAGCGCATGAGCATGGGTCGCGTGTGGTGATGACCAGGAACGGCGGCGGCAGGAAGCGGAAGAGGAGGAGGTCAGGAGCAAGAGCtAAGCAGGTAGGTCTCTATTATTTGATTCGTCAGCCTCCAGCCGGTACGGAGCACTGTCGCCGCAGTTGAGGCTCAAGGACGCCAAGGACATCGCGTACTCGTACCCAGCGCACACCACTACTGGTCCTTGCCGCCGCCGTCCTTCGCCTTCTCCTCCTCCAGTCCTCGTTCTCGaTTCGTCCATCCPU6.4720740760780800820840860
    AGAACGAGGGAGCAGAGTACTTGTGGCTGGGAGGCCACCGGTGCCCTAGCAGGGGCACGCGCCGGTGACGTGAGCAGGAGGAGAAGGCAGTATACGCACTTGACACGGCTTCAGGAGGCGCACAGGAGGCGGAGGCCGCCACGAACCAGCAGAGGAAGCAATAAATTGTCTTACTTCTTGCTCCCTCGTCTCATGAACACCGACCCTCCGGTGGCCACGGGATCGTCCCCGTGCGCGGCCACTGCACTCGTCCTCCTCTTCCGTCATATGCGTGAACTGTGCCGAAGTCCTCCGCGTGTCCTCCGCCTCCGGCGGTGCTTGGTCGTCTCCTTCGTTATTTAACAGAATGAAgeIBarISgrAISgrAIBstZ17IPU6.48809009209409609801,0001,0201,040
    ATATATATATTTGGCCTATGAGAATTacttTGAGACGTACCATATGAACCGTCTCCgttttagtactctggaaacagaatctactaaaacaaggcaaaatgccgtgtttatctcgtcaacttgttggcgagattttttcccgggCTGCAGGAATTCGAtatcaagcttgcatgccTATATATATAAACCGGATACTCTTAAtgaaACTCTGCATGGTATACTTGGCAGAGGcaaaatcatgagacctttgtcttagatgattttgttccgttttacggcacaaatagagcagttgaacaaccgctctaaaaaagggcccGACGTCCTTAAGCTatagttcgaacgtacggNdeIPstISmaISphIXmaIBsmBIBsmBIEcoRIEcoRVEsp3IEsp3ITspMIHindIIISaCas9 ScaffoldPU6.4Cloning InsertRB...r1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttcccgcgcggtcttcctcgcgtcggtttggtcctactacaaactaccccataaactcgtgaacgttgggaataggccttcgggggaccgggtgtttccgatccgcggttacgttcgtcaagcgtacgtcggggacctcgccacgggaggactatttggccggtcccccggatacaaAscIKasINarISfoISphIBspEIPluTIRBCS2 Promotor1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ctttacttttttacaagagaagtcactcaacatcttaaaatggccaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgaaatgaaaaaatgttctcttcagtgagttgtagaattttaccggtccactcagctgctcgttcgggccgcctagtccgtcgcacgaacgtctaaactgaacgttgcgggcgtaacacagctgcttccgaaaaccgaggagacagcgacagagttcgtcgtagattgggacgcagMscIRbcS2 Intron15´UTR RBCS2RBCS2...otor1,4201,4401,4601,4801,5001,5201,5401,560
    gccgtttccatttgcaggatgctcgagattcgaagcatggacgatgcgttgcgtgcactgcggggtcggtatcccggttgtgagtgggttgttgtggaggatggggcctcgggggctggtgtttatcggcttcggggtggtgggcgggagttgtttgtcaaggtggcagctctggcggcaaaggtaaacgtcctacgagctctaagcttcgtacctgctacgcaacgcacgtgacgccccagccatagggccaacactcacccaacaacacctcctaccccggagcccccgaccacaaatagccgaagccccaccacccgccctcaacaaacagttccaccgtcgagaccTliIXhoIBstBIPaeR7IAPHVIIIRbcS2...ron11,5801,6001,6201,6401,6601,6801,7001,7201,740
    gggccggggtgggcttgttgggtgaggctgagcggctggtgtggttggcggaggtggggattcccgtacctcgtgttgtggagggtggtggggacgagagggtcgcctggttggtcaccgaagcggttccggggcgtccggccagtgcgcggtggccgcgggagcagcggctggacccggccccacccgaacaacccactccgactcgccgaccacaccaaccgcctccacccctaagggcatggagcacaacacctcccaccacccctgctctcccagcggaccaaccagtggcttcgccaaggccccgcaggccggtcacgcgccaccggcgccctcgtcgccgacctXmnIAPHVIII1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgtggcggtggcgctcgcggggctcgctcgttcgctgcacgcgctggactgggagcggtgtccgttcgatcgcagtctcgcggtgacggtgccgcaggcggcccgtgctgtcgctgaagggagcgtcgacttggaggatctggacgaggagcggaaggggtggtcgggggagcgggcaccgccaccgcgagcgccccgagcgagcaagcgacgtgcgcgacctgaccctcgccacaggcaagctagcgtcagagcgccactgccacggcgtccgccgggcacgacagcgacttccctcgcagctgaacctcctagacctgctcctcgccttccccaccagccccctcgccBsgIAPHVIII1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cttctcgccgagctggagcggactcggcctgcggacgaggatctggcggtttgccacggtgacctgtgcccggacaacgtgctgctcgaccctcgtacctgcgaggtgaccgggctgatcgacgtggggcgggtcggccgtgcggaccggcactccgatctcgcgctggtgctgcgaagagcggctcgacctcgcctgagccggacgcctgctcctagaccgccaaacggtgccactggacacgggcctgttgcacgacgagctgggagcatggacgctccactggcccgactagctgcaccccgcccagccggcacgcctggccgtgaggctagagcgcgaccacgacgBaeIEagIRsrIIAPHVIII2,1202,1402,1602,1802,2002,2202,2402,260
    gcgagctggcccacgaggaggacccgtggttcgggccggagtgttccgcggcgttcctgcgggagtacgggcgcgggtgggatggggcggtatcggaggaaaagctggcgttttaccggctgttggacgagttcttctgagggacctgatggtgttggtggctgggtagggttgccgctcgaccgggtgctcctcctgggcaccaagcccggcctcacaaggcgccgcaaggacgccctcatgcccgcgcccaccctaccccgccatagcctccttttcgaccgcaaaatggccgacaacctgctcaagaagactccctggactaccacaaccaccgacccatcccaacgAloIPpuMIPpuMIAPHVIII2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtcgcgtgggtgacagcacagtgtggacgttgggatccccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacaccagcgcacccactgtcgtgtcacacctgcaaccctaggggcgaggcacatttacctccgcgagcaactagactcggaacgggggactgcttgccgccacctaccttctatgacgagagttcacgacttcgccatcgaatcgaggggcaaagcacgactagtcagaaaaagttgtgBclIBamHIAflIIIRbcs2\Term.2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcgggtacccagcttttgttccctttagtgagggttaattGCGCgcttggcgtaatcatggtcatagctgtttcctgtgtgcatttttcgcctcctcaaaacgttaaaacaaccaacattgctaggaggcaactaaaaccggagaaagaggtacccgcccgacccgcataaacttcgcccatgggtcgaaaacaagggaaatcactcccaattaaCGCGcgaaccgcattagtaccagtatcgacaaaggacacacKpnINcoIStyIXcmIBsaAIAcc65IRbcs2\Term.Terminator HairpinM13-revT3T3 promoter2,6402,6602,6802,7002,7202,7402,7602,7802,800
    aaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggtttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgccccPvuIIlac promoterLacO2,8202,8402,8602,8802,9002,9202,9402,960
    agaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccatctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtPciISapIBspQIAflIII2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaColE1 origin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgColE1 origin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcColE1 origin3,5203,5403,5603,5803,6003,6203,6403,660
    ttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaaColE1 origin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagtagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatcAhdIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggtattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccBsaIBsrDIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccAclIFspIBsrDIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    tcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccgaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggcAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttcgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaaAclIXmnIAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtgacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaSspI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaPsiISspICspCIF1 ori4,9204,9404,9604,9805,0005,0205,0405,060
    ggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgAloIF1 ori5,0805,1005,1205,140
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    5143 bp37504000425045004750500025050075010001250RBCS2 Promotoraaac

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    5143