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XZ076 (PB)CMV-mCCL20 (PuroR-BFP)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHI20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIAmp180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIAmp360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataAmp8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaBspHICspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggAloI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcatt1,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagPvuI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataaBII(R)5'TR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataatttttttt31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgccccore insul2,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctataMfeISfiISrfIBpu10Icore insulPCRhomolog2,2802,3002,3202,3402,3602,3802,4002,4202,440
    acgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattMluISpeIAflIIICMVCMV promoter (with TetOs)enhancer2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacNdeICMVCMV promoter (with TetOs)enhancer2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgCspCISnaBICMVCMV promoter (with TetOs)core promoterenhancerEKo209.IVT-32,8202,8402,8602,8802,9002,9202,9402,960
    aactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggBglIIBsaBIBsaBIBsmBIEsp3ISgrDICMV promoter (with TetOs)CMVcore promoterTetR binding sitesTetOTetOEKo209.IVT-12,9803,0003,0203,0403,0603,0803,1003,1203,140
    atagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccATGTGCTGTACCAAGAGTTTGCTCCTGGCTGCTTTGATGTCAGTGCTGCTACTCCACCTCTGCGGCGAATCAGAAGCAGCAAGCAACTTTGACTGCTGTCTTGGATACACAGtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggTACACGACATGGTTCTCAAACGAGGACCGACGAAACTACAGTCACGACGATGAGGTGGAGACGCCGCTTAGTCTTCGTCGTTCGTTGAAACTGACGACAGAACCTATGTGTCPmeIAflIIPshAIHindIIIMC2CT4KS6LL8LA10AL12MS14VL16LL18HL20CG22ES24EA26AS28NF30DC32CL34GY36T3214-4527Ccl20CMV promoter (with TetOs)homolog3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ACCGTATTCTTCATCCTAAATTTATTGTGGGCTTCACACGGCAGCTGGCCAATGAAGGCTGTGACATCAATGCTATCATCTTTCACACAAAGAAAAAGTTGTCTGTGTGCGCAAATCCAAAACAGACTTGGGTGAAATATATTGTGCGTCTCCTCAGTAAAAAAGTCAAGAACATTGGCATAAGAAGTAGGATTTAAATAACACCCGAAGTGTGCCGTCGACCGGTTACTTCCGACACTGTAGTTACGATAGTAGAAAGTGTGTTTCTTTTTCAACAGACACACGCGTTTAGGTTTTGTCTGAACCCACTTTATATAACACGCAGAGGAGTCATTTTTTCAGTTCTTGTABaeIXcmIBsmBIEsp3ID38RI40LH42PK44FI46VG48FT50RQ52LA54NE56GC58DI60NA62II64FH66TK68KK70LS72VC74AN76PK78QT80WV82KY84IV86RL88LS90KK92VK94NM963214-4527Ccl203,3403,3603,3803,4003,4203,4403,4603,4803,500
    GGGTACCGGGAACGGAAATGGCAATGGTAACGGGAATGGAAACGGCAATGGTAATGGGAATACTAGTGTTAACACCGGTGCGGCCGTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACCCCATGGCCCTTGCCTTTACCGTTACCATTGCCCTTACCTTTGCCGTTACCATTACCCTTATGATCACAATTGTGGCCACGCCGGCAAATGGGTATGCTACAAGGACTGATACGCCCGATAGGGATACTGCAGGGCCTGATACGTCCTAGGATAGGTATACTGCAAGGTCTAATGAgeIHpaIKpnINdeISpeIBamHISgrAIAcc65IGT98GN100GN102GN104GN106GN108GN110GN112GN114GN116TS118VN120TG122AA124VY126PY128DV130PD132YA134GY136PY138DV140PD142YA144GS146YP148YD150VP152DY1543214-45273xHAflexible linker3,5203,5403,5603,5803,6003,6203,6403,660
    GCTCCGGCCGCCACCGGTCTTAAGGTCGACTTTCAGAAAGCTAGCTCTATTGTTTATAAGAAGGAGGGGGAACAGGTGGAGTTCAGTTTCCCATTGGCCTTCACAGTCGAGAAGCTCACAGGATCAGGAGAGTTGTGGTGGCAGGCCGAAAGAGCCTCCTCCAGCAAGAGTTGGACGAGGCCGGCGGTGGCCAGAATTCCAGCTGAAAGTCTTTCGATCGAGATAACAAATATTCTTCCTCCCCCTTGTCCACCTCAAGTCAAAGGGTAACCGGAAGTGTCAGCTCTTCGAGTGTCCTAGTCCTCTCAACACCACCGTCCGGCTTTCTCGGAGGAGGTCGTTCTCAACCTAgeIBmtINheIAflIIAP156AA158TG160LK162VD164FQ166KA168SS170IV172YK174KE176GE178QV180EF182SF184PL186AF188TV190EK192LT194GS196GE198LW200WQ202AE204RA206SS208SK210SW2123214-4527CD4pre mgrasp3xHA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TCACTTTTGATCTGAAAAACAAAGAAGTGAGCGTGAAGAGAGTCACACAGGACCCTAAGCTGCAGATGGGCAAGAAGTTGCCTTTGCACTTGACACTCCCCCAAGCCCTGCCCCAGTATGCTGGCTCCGGGAACCTCACCCTCGCCTTGGAGGCTAAGACCGGAAAGTTGCATCAAGTGAAAACTAGACTTTTTGTTTCTTCACTCGCACTTCTCTCAGTGTGTCCTGGGATTCGACGTCTACCCGTTCTTCAACGGAAACGTGAACTGTGAGGGGGTTCGGGACGGGGTCATACGACCGAGGCCCTTGGAGTGGGAGCGGAACCTCCGATTCTGGCCTTTCAACGTAGTBstXIBpu10IIT214FD216LK218NK220EV222SV224KR226VT228QD230PK232LQ234MG236KK238LP240LH242LT244LP246QA248LP250QY252AG254SG256NL258TL260AL262EA264KT266GK268LH270Q3214-4527CD4pre mgrasp3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GGAGGTCAACCTCGTGGTGATGAGGGCAACACAGCTGCAGAAGAACCTGACCTGCGAAGTGTGGGGTCCCACCTCACCTAAACTGATGCTGTCTCTGAAGCTGGAGAATAAGGAGGCTAAAGTCAGTAAAAGAGAGAAGGCTGTGTGGGTCCTTAACCCGGAGGCAGGGATGTGGCCTCCAGTTGGAGCACCACTACTCCCGTTGTGTCGACGTCTTCTTGGACTGGACGCTTCACACCCCAGGGTGGAGTGGATTTGACTACGACAGAGACTTCGACCTCTTATTCCTCCGATTTCAGTCATTTTCTCTCTTCCGACACACCCAGGAATTGGGCCTCCGTCCCTACACCKflIBfuAIBspMIE272VN274LV276VM278RA280TQ282LQ284KN286LT288CE290VW292GP294TS296PK298LM300LS302LK304LE306NK308EA310KV312SK314RE316KA318VW320VL322NP324EA326GM328W3214-4527CD4pre mgrasp4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CAGTGTCTTCTTAGCGACTCTGGACAGGTGCTGCTGGAGAGCAACATCAAGGTGCTTCCTACTTGGTCAACACCAGTACAGCCGATGGCCCTGATCGTACTCGGGGGAGTCGCCGGCCTGCTTCTGTTTATCGGCCTGGGCATCTTCTTCTGCGTACGCTACAAGTATCGCAACAGTCACAGAAGAATCGCTGAGACCTGTCCACGACGACCTCTCGTTGTAGTTCCACGAAGGATGAACCAGTTGTGGTCATGTCGGCTACCGGGACTAGCATGAGCCCCCTCAGCGGCCGGACGAAGACAAATAGCCGGACCCGTAGAAGAAGACGCATGCGATGTTCATAGCGTTGTQ330CL332LS334DS336GQ338VL340LE342SN344IK346VL348PT350WS352TP354VQ356PM358AL360IV362LG364GV366AG368LL370LF372IG374LG376IF378FC380VR382YK384YR386N3214-4527pre mgraspCD4NXN c...gion4,2204,2404,2604,2804,3004,3204,3404,360
    GGGATGAGGGATCTTACCACGTTGACGAATCTAGGAACTATATCAGTAACAGCGCTCAGAGTAATGGCGCCGTGGTTAAAGAGAAACAGCCCTCCAGCGCCAAATCAGCAAACAAAAACAAGAAAAACAAGGATAAAGAGTACTACGTAtaggccgctcgaggagggcccgaacaCCCTACTCCCTAGAATGGTGCAACTGCTTAGATCCTTGATATAGTCATTGTCGCGAGTCTCATTACCGCGGCACCAATTTCTCTTTGTCGGGAGGTCGCGGTTTAGTCGTTTGTTTTTGTTCTTTTTGTTCCTATTTCTCATGATGCATatccggcgagctcctcccgggcttgtAfeIApaIScaITliIXhoIPspXISnaBIPaeR7IPspOMIR388DE390GS392YH394VD396ES398RN400YI402SN404SA406QS408NG410AV412VK414EK416QP418SS420AK422SA424NK426NK428KN430KD432KE434YY436V*4383214-4527pre mgraspNXN cytoplasmic regionhomolog4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttccttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggBclIPmeIBGH polyadenylation sequencec-myc epitopePolyhi...e tag4,5604,5804,6004,6204,6404,6604,6804,7004,720
    taataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtgattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacNotIXmnIBGH polyadenylation sequence5, 299 Fhomolog4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgpSV404,9204,9404,9604,9805,0005,0205,0405,060
    tccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgataggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactaBclIAvrIIpSV405,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagcacgtgATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCgtcgtgcacTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGPmlIPflFIBstEIITth111IMT2EY4KP6TV8RL10AT12RD14DV16PR18AV20RT22LA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL52FL54TpuroRPuroRPR_S030PR_S0295,2605,2805,3005,3205,3405,3605,3805,4005,420
    GCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGR56VG58LD60IG62KV64WV66AD68DG70AA72VA74VW76TT78PE80SV82EA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME110GL112LA114puroRPuroR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCBcgIPH116RP118KE120PA122WF124LA126TV128GV130SP132DH134QG136KG138LG140SA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL168PF170YE172puroRPuroR5,6205,6405,6605,6805,7005,7205,7405,760
    CGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattccSapIBamHIBfuAIBmgBIBspMIBspQIPaqCIRL174GF176TV178TA180DV182EV184PE186GP188RT190WC192MT194RK196PG198AA200TN202FS204LL206KQ208AG210DV212EE214NP216GP218GS220MV222SK224GE226EL228IK230puroRPuroRP2AmtagBFP2homologous5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggtcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccBsrGIEN232MH234MK236LY238ME240GT242VD244NH246HF248KC250TS252EG254EG256KP258YE260GT262QT264MR266IK268VV270EG272GP274LP276FA278FD280IL282AT284SF286LY288GpuroRmtagBFP25,9605,9806,0006,0206,0406,0606,0806,1006,120
    cagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctcccAloIBsu36IS290KT292FI294NH296TQ298GI300PD302FF304KQ306SF308PE310GF312TW314ER316VT318TY320ED322GG324VL326TA328TQ330DT332SL334QD336GC338LI340YN342VK344IR346GpuroRmtagBFP26,1406,1606,1806,2006,2206,2406,2606,2806,300
    gtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagacacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctV348NF350TS352NG354PV356MQ358KK360TL362GW364EA366FT368ET370LY372PA374DG376GL378EG380RN382DM384AL386KL388VG390GS392HL394IA396NA398KT400TY402RS404KpuroRmtagBFP26,3206,3406,3606,3806,4006,4206,4406,460
    aacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAgtcgacaatcaacctctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTcagctgttagttggagaPacIK406PA408KN410LK412MP414GV416YY418VD420YR422LE424RI426KE428AN430NE432TY434VE436QH438EV440AV442AR444YC446DL448PS450KL452GH454KL456N*458puroRmtagBFP2homologWPRE6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattcctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaHpaIWPRESV40 pASV40 pA(1)homolog6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatBsmISV40 pASV40 pA(1)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tatcccttgctctggtcaaccaggttgcagggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcaatagggaacgagaccagttggtccaacgtcccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtApaIBstXIEcoNIPspOMIcore insul(1) Gene...me: core insul(1)7,0207,0407,0607,0807,1007,1207,1407,160
    gcgagaagcgttcagaggaaagcgatcccgtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggcgctcttcgcaagtctcctttcgctagggcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccSrfIcore insul(1) Geneious name: core insul(1)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ggggggctgtccctgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacccccccgacagggactatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgEcoRVcore...(1)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagBsmI3'TR7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagccc7,7207,7407,7607,7807,8007,8207,8407,860
    aaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtc7,8807,9007,9207,9407,9607,9808,0008,0208,040
    cggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggcBsgIpLac(F)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgaSapIBspQI8,2408,2608,2808,3008,3208,3408,3608,3808,400
    cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagggtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccPciIAflIII8,4208,4408,4608,4808,5008,5208,5408,560
    ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtgggaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcacc8,5808,6008,6208,6408,6608,6808,7008,7208,740
    cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgggcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgacc8,7608,7808,8008,8208,8408,8608,8808,9008,920
    taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcg8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtt9,1209,140
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9154 bp65007000750080008500500100015002000core insulSV40 pA(1)ES003-1 C->AAmppLac(F)EKo152.2 (DNA)pAAV-F (DNA)EKo272.2 (DNA)EKbF (DNA)pNK018 (DNA)XZp075.1 (DNA)M13-48REV Genewiz (DNA)BspHI,AhdIAclI,PvuIScaI,BcgIAclI,XmnIBspHICspCIAloIFrtF (DNA)FlpInR (DNA)M13-40FOR Genewiz (DNA),PvuIM13F Genewiz (DNA)BII(R),5'TR,31bp spacer,5'TR(1)5'TR5'TRYX021-seq-3 (DNA)SrfIEWXglobF NONSPECIFIC DONT USE (DNA),+1EcoNI,PspOMI,ApaI,BstXIEWXglobF NONSPECIFIC DONT USE (DNA)core insul(1) Geneious name: core insul(1),SrfIEcoRV3'TR,BsmIES003-3 C->ABsgIM13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapI,AflIII,PciIpUCoriR (DNA)

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    9154