Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
BCL6-GFP in Artichoke MGH sequencing
  • Sequence Map
    Editing disabled on read-only sequence.
    GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGC20406080100120140160
    CCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACT180200220240260280300320340
    TTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTG360380400420440460480500520
    TGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGTTCGCGCGTTAATTGGGAGlac promoterLacOM13-revT3...rT3540560580600620640660680700
    ACTAAAGGGAACAAAAGCTGGAGCTGCAAGCTTAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAATGATTTCCCTTGTTTTCGACCTCGACGTTCGAATTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGCACGGAATAATCCTTCCGTTBaeISphI5'LTRT3720740760780800820840860
    CAGACGGGTCTGACATGGATTGGACGAACCACTGAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAA5'LTRHIV R8809009209409609801,0001,0201,040
    GAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCCTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCGCG5'LTRHIV U5psiPSI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATNruIPSIpsiDelta GAGgagMaj...or1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTADelta GAGgag1,4201,4401,4601,4801,5001,5201,5401,560
    AGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACAGCAAGCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTTCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGCGTGTCGTTCGCCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAMfeINotIgagDelta GAG1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTCGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTARRE1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAAACGACTCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATRRE1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAACAACCTCATTATTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTT2,1202,1402,1602,1802,2002,2202,2402,260
    TTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCTATTCCAACCAAATTGTATTGTTTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGATAAGGKflIPpuMI2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AGCACATATGAGCTAGCTGCAGTAACGCCATTTTGCAAGGCATGGAAAAATACCAAACCAAGAATAGAGAAGTTCAGATCAAGGGCGGGTACATGAAAATAGCTAACGTTGGGCCAAACAGGATATCTGCGGTGAGCAGTTTCGGCCCCGGCCCGGGGCCAAGAACAGATGGTCATCGTGTATACTCGATCGACGTCATTGCGGTAAAACGTTCCGTACCTTTTTATGGTTTGGTTCTTATCTCTTCAAGTCTAGTTCCCGCCCATGTACTTTTATCGATTGCAACCCGGTTTGTCCTATAGACGCCACTCGTCAAAGCCGGGGCCGGGCCCCGGTTCTTGTCTACCAGTBmtINdeINheISmaIXmaIEcoRVTspMISFFV2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CCGCAGTTTCGGCCCCGGCCCGAGGCCAAGAACAGATGGTCCCCAGATATGGCCCAACCCTCAGCAGTTTCTTAAGACCCATCAGATGTTTCCAGGCTCCCCCAAGGACCTGAAATGACCCTGCGCCTTATTTGAATTAACCAATCAGCCTGCTTCTCGCTTCTGTTCGCGCGCTGGCGTCAAAGCCGGGGCCGGGCTCCGGTTCTTGTCTACCAGGGGTCTATACCGGGTTGGGAGTCGTCAAAGAATTCTGGGTAGTCTACAAAGGTCCGAGGGGGTTCCTGGACTTTACTGGGACGCGGAATAAACTTAATTGGTTAGTCGGACGAAGAGCGAAGACAAGCGCGCGAPpuMISFFV2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TCTGCTTCCCGAGCTCTATAAAAGAGCTCACAACCCCTCACTCGGCGCGCCAGTCCTCCGACAGACTGAGTCGGCCGGTCGAATCAAGCTTATCGATACCGTCGACTCCGGAATAGCCACCATGGAGACGGATCAAACAAGTTTGTACAAAAAAGTTGGCACCATGGCCTCGCCGAGACGAAGGGCTCGAGATATTTTCTCGAGTGTTGGGGAGTGAGCCGCGCGGTCAGGAGGCTGTCTGACTCAGCCGGCCAGCTTAGTTCGAATAGCTATGGCAGCTGAGGCCTTATCGGTGGTACCTCTGCCTAGTTTGTTCAAACATGTTTTTTCAACCGTGGTACCGGAGCGGCAscIBaeIClaIBspDIMA2SP42964-5085SFFVGateway recomb1attB1BCL6Insert2,8202,8402,8602,8802,9002,9202,9402,960
    GCTGACAGCTGTATCCAGTTCACCCGCCATGCCAGTGATGTTCTTCTCAACCTTAATCGTCTCCGGAGTCGAGACATCTTGACTGATGTTGTCATTGTTGTGAGCCGTGAGCAGTTTAGAGCCCATAAAACGGTCCTCATGGCCTGCAGTGGCCTGTTCTATAGCATCTTTACAGCGACTGTCGACATAGGTCAAGTGGGCGGTACGGTCACTACAAGAAGAGTTGGAATTAGCAGAGGCCTCAGCTCTGTAGAACTGACTACAACAGTAACAACACTCGGCACTCGTCAAATCTCGGGTATTTTGCCAGGAGTACCGGACGTCACCGGACAAGATATCGTAGAAATGTCAD6SC8IQ10FT12RH14AS16DV18LL20NL22NR24LR26SR28DI30LT32DV34VI36VV38SR40EQ42FR44AH46KT48VL50MA52CS54GL56FY58SI60FT622964-5085BCL6Insert2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACCAGTTGAAATGCAACCTTAGTGTGATCAATCTAGATCCTGAGATCAACCCTGAGGGATTCTGCATCCTCCTGGACTTCATGTACACATCTCGGCTCAATTTGCGGGAGGGCAACATCATGGCTGTGATGGCCACGGCTATGTACCTGCAGATGGAGCATGTTGTGGACACTTGTGGTCAACTTTACGTTGGAATCACACTAGTTAGATCTAGGACTCTAGTTGGGACTCCCTAAGACGTAGGAGGACCTGAAGTACATGTGTAGAGCCGAGTTAAACGCCCTCCCGTTGTAGTACCGACACTACCGGTGCCGATACATGGACGTCTACCTCGTACAACACCTGTGAACBclIXbaIBsu36IDQ64LK66CN68LS70VI72NL74DP76EI78NP80EG82FC84IL86LD88FM90YT92SR94LN96LR98EG100NI102MA104VM106AT108AM110YL112QM114EH116VV118DT120C2964-5085BCL6Insert3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCGGAAGTTTATTAAGGCCAGTGAAGCAGAGATGGTTTCTGCCATCAAGCCTCCTCGTGAAGAGTTCCTCAACAGCCGGATGCTGATGCCCCAAGACATCATGGCCTATCGGGGTTGTGAGGTGGTGGAGAACAACCTGCCACTGAGGAGCGCCCCTGGGTGTGAGAGCAGAGCCGGCCTTCAAATAATTCCGGTCACTTCGTCTCTACCAAAGACGGTAGTTCGGAGGAGCACTTCTCAAGGAGTTGTCGGCCTACGACTACGGGGTTCTGTAGTACCGGATAGCCCCAACACTCCACCACCTCTTGTTGGACGGTGACTCCTCGCGGGGACCCACACTCTCGTCTCGGR122KF124IK126AS128EA130EM132VS134AI136KP138PR140EE142FL144NS146RM148LM150PQ152DI154MA156YR158GC160EV162VE164NN166LP168LR170SA172PG174CE176SR178A2964-5085BCL6Insert3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TTTGCCCCCAGCCTGTACAGTGGCCTGTCCACACCGCCAGCCTCTTATTCCATGTACAGCCACCTCCCTGTCAGCAGCCTCCTCTTCTCCGATGAGGAGTTTCGGGATGTCCGGATGCCTGTGGCCAACCCCTTCCCCAAGGAGCGGGCACTCCCATGTGATAGTGCCAGGCCAGAAACGGGGGTCGGACATGTCACCGGACAGGTGTGGCGGTCGGAGAATAAGGTACATGTCGGTGGAGGGACAGTCGTCGGAGGAGAAGAGGCTACTCCTCAAAGCCCTACAGGCCTACGGACACCGGTTGGGGAAGGGGTTCCTCGCCCGTGAGGGTACACTATCACGGTCCGGTCF180AP182SL184YS186GL188ST190PP192AS194YS196MY198SH200LP202VS204SL206LF208SD210EE212FR214DV216RM218PV220AN222PF224PK226ER228AL230PC232DS234AR236P2964-5085BCL6Insert3,5203,5403,5603,5803,6003,6203,6403,660
    TCCCTGGTGAGTACAGCCGGCCGACTTTGGAGGTGTCCCCCAATGTGTGCCACAGCAATATCTATTCACCCAAGGAAACAATCCCAGAAGAGGCACGAAGTGATATGCACTACAGTGTGGCTGAGGGCCTCAAACCTGCTGCCCCCTCAGCCCGAAATGCCCCCTACTTCCCTTGAGGGACCACTCATGTCGGCCGGCTGAAACCTCCACAGGGGGTTACACACGGTGTCGTTATAGATAAGTGGGTTCCTTTGTTAGGGTCTTCTCCGTGCTTCACTATACGTGATGTCACACCGACTCCCGGAGTTTGGACGACGGGGGAGTCGGGCTTTACGGGGGATGAAGGGAACV238PG240EY242SR244PT246LE248VS250PN252VC254HS256NI258YS260PK262ET264IP266EE268AR270SD272MH274YS276VA278EG280LK282PA284AP286SA288RN290AP292YF294PC2962964-5085BCL6Insert3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGACAAGGCCAGCAAAGAAGAAGAGAGACCCTCCTCGGAAGATGAGATTGCCCTGCATTTCGAGCCCCCCAATGCACCCCTGAACCGGAAGGGTCTGGTTAGTCCACAGAGCCCCCAGAAATCTGACTGCCAGCCCAACTCGCCCACAGAGTCCTGCAGCAGTAAGAATGCCTGCACTGTTCCGGTCGTTTCTTCTTCTCTCTGGGAGGAGCCTTCTACTCTAACGGGACGTAAAGCTCGGGGGGTTACGTGGGGACTTGGCCTTCCCAGACCAATCAGGTGTCTCGGGGGTCTTTAGACTGACGGTCGGGTTGAGCGGGTGTCTCAGGACGTCGTCATTCTTACGGACGDK298AS300KE302EE304RP306SS308ED310EI312AL314HF316EP318PN320AP322LN324RK326GL328VS330PQ332SP334QK336SD338CQ340PN342SP344TE346SC348SS350KN352AC3542964-5085BCL6Insert3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCCCACTGACCCCAAAGCCTGCAACTGGAAGAAATACAAGTTCATCGTGCTCAACAGCCTCAATCAGAATGCCAAACCAGAGGGGCCTGAGCAGGCTGAGCTGGGCCGCCTTTCCCCACGAGCCTACACGGCCCCACCTGTAGGAGGTCCGAAGACCGAGGGGAGGTCGGTTCTCGGGGTGACTGGGGTTTCGGACGTTGACCTTCTTTATGTTCAAGTAGCACGAGTTGTCGGAGTTAGTCTTACGGTTTGGTCTCCCCGGACTCGTCCGACTCGACCCGGCGGAAAGGGGTGCTCGGATGTGCCGGGGTGGACBlpIIL356QA358SG360SP362PA364KS366PT368DP370KA372CN374WK376KY378KF380IV382LN384SL386NQ388NA390KP392EG394PE396QA398EL400GR402LS404PR406AY408TA410PP4122964-5085BCL6Insert4,0404,0604,0804,1004,1204,1404,1604,1804,200
    CCTGCCAGCCACCCATGGAGCCTGAGAACCTTGACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCATCCCACAAGCCAGCCGGCTCAATAACATCGTTAACAGGTCCATGACGGGCTCTCCCCGCAGCAGCAGCGAGAGCCACTCACCACTCTACATGCAGGACGGTCGGTGGGTACCTCGGACTCTTGGAACTGGAGGTCAGGGGTTGGTTCGACTCACGGTCGCCCCTCCTGAGGTGGTAGGGTGTTCGGTCGGCCGAGTTATTGTAGCAATTGTCCAGGTACTGCCCGAGAGGGGCGTCGTCGTCGCTCTCGGTGAGTGGTGAGATGTACGTHpaIAC414QP416PM418EP420EN422LD424LQ426SP428TK430LS432AS434GE436DS438TI440PQ442AS444RL446NN448IV450NR452SM454TG456SP458RS460SS462ES464HS466PL468YM470H2964-5085BCL6Insert4,2204,2404,2604,2804,3004,3204,3404,360
    CCCCCCGAAGTGCACGTCCTGCGGCTCTCAGTCCCCACAGCATGCAGAGATGTGCCTCCACACCGCTGGCCCCACGTTCCCTGAGGAGATGGGAGAGACCCAGTCTGAGTACTCAGATTCTAGCTGTGAGAACGGGGCCTTCTTCTGCAATGAGTGTGACTGCCGCTTCTCTGAGGGGGGGCTTCACGTGCAGGACGCCGAGAGTCAGGGGTGTCGTACGTCTCTACACGGAGGTGTGGCGACCGGGGTGCAAGGGACTCCTCTACCCTCTCTGGGTCAGACTCATGAGTCTAAGATCGACACTCTTGCCCCGGAAGAAGACGTTACTCACACTGACGGCGAAGAGACTCScaISphIBmgBIBsu36IP472PK474CT476SC478GS480QS482PQ484HA486EM488CL490HT492AG494PT496FP498EE500MG502ET504QS506EY508SD510SS512CE514NG516AF518FC520NE522CD524CR526FS528E2964-5085BCL6Insert4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GAGGCCTCACTCAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACCGCTGCCAGGCCTCCTTCCGCTACAAGGGCAACCTCGCCAGCCACAAGACCGTCCATACCGGTGAGAAACCCTATCGTTGCAACATCTGTGGGGCCCAGTTCAACCGGCCAGCCACTCCGGAGTGAGTTCTCCGTGTGCGACGTCTGGGTGTCACTGTTTGGGATGTTCACACTGGCGACGGTCCGGAGGAAGGCGATGTTCCCGTTGGAGCGGTCGGTGTTCTGGCAGGTATGGCCACTCTTTGGGATAGCAACGTTGTAGACACCCCGGGTCAAGTTGGCCGGTCGGTAgeICspCIE530AS532LK534RH536TL538QT540HS542DK544PY546KC548DR550CQ552AS554FR556YK558GN560LA562SH564KT566VH568TG570EK572PY574RC576NI578CG580AQ582FN584RP586A2964-5085BCL6Insert4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ACCTGAAAACCCACACTCGAATTCACTCTGGAGAGAAGCCCTACAAATGCGAAACCTGCGGAGCCAGATTTGTACAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGAAGCCCTATCCCTGTGAAATCTGTGGCACCCGTTTCCGGCACCTTCAGACTCTGAATGGACTTTTGGGTGTGAGCTTAAGTGAGACCTCTCTTCGGGATGTTTACGCTTTGGACGCCTCGGTCTAAACATGTCCACCGGGTGGAGGCACGGGTACACGAATAGGTGTGACCACTCTTCGGGATAGGGACACTTTAGACACCGTGGGCAAAGGCCGTGGAAGTCTGAGACTTEcoRIN588LK590TH592TR594IH596SG598EK600PY602KC604ET606CG608AR610FV612QV614AH616LR618AH620VL622IH624TG626EK628PY630PC632EI634CG636TR638FR640HL642QT644LK6462964-5085BCL6Insert4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GAGCCACCTGCGAATCCACACAGGAGAGAAACCTTACCATTGTGAGAAGTGTAACCTGCATTTCCGTCACAAAAGCCAGCTGCGACTTCACTTGCGCCAGAAGCATGGCGCCATCACCAACACCAAGGTGCAATACCGCGTGTCAGCCACTGACCTGCCTCCGGAGCTCCCCAAACTCGGTGGACGCTTAGGTGTGTCCTCTCTTTGGAATGGTAACACTCTTCACATTGGACGTAAAGGCAGTGTTTTCGGTCGACGCTGAAGTGAACGCGGTCTTCGTACCGCGGTAGTGGTTGTGGTTCCACGTTATGGCGCACAGTCGGTGACTGGACGGAGGCCTCGAGGGGTTTSH648LR650IH652TG654EK656PY658HC660EK662CN664LH666FR668HK670SQ672LR674LH676LR678QK680HG682AI684TN686TK688VQ690YR692VS694AT696DL698PP700EL702PK7042964-5085BCL6Insert4,9204,9404,9604,9805,0005,0205,0405,060
    GCCTGCTTGCCAACTTTCTTGTACAAAGTGGTTCGATACGTCTCAGCTGAAGCTGCTGCAAAGGAAGCTGCAGCTAAGGAGGCTGCAGCTAAGGCTGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTCGGACGAACGGTTGAAAGAACATGTTTCACCAAGCTATGCAGAGTCGACTTCGACGACGTTTCCTTCGACGTCGATTCCTCCGACGTCGATTCCGACACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAP1T2F3L4Y5K6V7V8R9Y10V11S12A13E14A15A16A17K18E19A20A21A22K23E24A25A26A27K28A295085-5171AC706LVS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24HK265172-5888EGFPRigid LinkerInsertGateway Recomb2attB2BCL65,0805,1005,1205,1405,1605,1805,2005,2205,240
    TCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTFS28VS30GE32GE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF84K5172-5888EGFP5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGS86AM88PE90GY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE142Y5172-5888EGFP5,4405,4605,4805,5005,5205,5405,5605,5805,600
    AACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGATTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTN144YN146SH148NV150YI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY200L5172-5888EGFP5,6205,6405,6605,6805,7005,7205,7405,760
    GCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAATGCATGAGTAACTGAGGATCCGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTTACGTACTCATTGACTCCTAGGCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCNsiIBamHIS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK238*5172-5888EGFPIRES5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTAvrIIIRES5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCPmlIIRES6,1406,1606,1806,2006,2206,2406,2606,2806,300
    TCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTACACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCATGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGKpnIBmgBIAcc65IIRES6,3206,3406,3606,3806,4006,4206,4406,460
    ACGATGATAATATGGCCACAACCCTGGAATTCGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACTGCTACTATTATACCGGTGTTGGGACCTTAAGCGGTGGTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGEcoRImCherryIRES6,4806,5006,5206,5406,5606,5806,6006,6206,640
    CCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAGGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGmCherry6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGACTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTSbfImCherry6,8406,8606,8806,9006,9206,9406,9606,9807,000
    AGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCmCherry7,0207,0407,0607,0807,1007,1207,1407,160
    CGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAAACTAGTAAGCTTGGCGTAACTAGATCTTGAGACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACAGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCATTTGATCATTCGAACCGCATTGATCTAGAACTCTGTTTACCGTCATAAGTAGGTGTTAAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCATCTGTATTATCGTTGTCTGTSpeIXcmISgrAIcpptmCherry7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TACAAACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAGCAGAGATCCACTTTGGGCTCGAGGGGGCCCGGGTGCAAAGATGGATAAAGTTTTAAACAGAGAGGAATCTTTGCAGCTAATGGACCTTCTAGGTCTTGAAAGGAGTGGATGTTTGATTTCTTAATGTTTTTGTTTAATGTTTTTAAGTTTTAAAAGCCCAAATAATGTCCCTGTCGTCTCTAGGTGAAACCCGAGCTCCCCCGGGCCCACGTTTCTACCTATTTCAAAATTTGTCTCTCCTTAGAAACGTCGATTACCTGGAAGATCCAGAACTTTCCTCACCSmaITliIXhoIXmaIPspXITspMIPaeR7IEF1acppt7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GAATTGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGATCCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTACTTAACCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTAGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATMfeIEF1a7,5407,5607,5807,6007,6207,6407,6607,6807,700
    TATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCEF1a5'UTR exon17,7207,7407,7607,7807,8007,8207,8407,860
    GTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACEF1a7,8807,9007,9207,9407,9607,9808,0008,0208,040
    CTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTEF1a8,0608,0808,1008,1208,1408,1608,1808,2008,220
    AGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCFseIEF1a8,2408,2608,2808,3008,3208,3408,3608,3808,400
    TCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTliIXhoIPaeR7IEF1a8,4208,4408,4608,4808,5008,5208,5408,560
    AGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGACGTACGGCCACCATGACCGAGTACAAGCCCACGGTGCGCCTTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTGCATGCCGGTGGTACTGGCTCATGTTCGGGTGCCACGCGGABsiWIEF1aPuro8,5808,6008,6208,6408,6608,6808,7008,7208,740
    CGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGBsiWIRsrIIPuro8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTPuro8,9408,9608,9809,0009,0209,0409,0609,0809,100
    CCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGPuro9,1209,1409,1609,1809,2009,2209,2409,260
    CGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTAAGTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAATTCAGCTGTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAMluISexAIWPREPuro9,2809,3009,3209,3409,3609,3809,4009,4209,440
    TCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAWPRE9,4609,4809,5009,5209,5409,5609,5809,6009,620
    TCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTTAGTAGCAGGAAAGGAACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATWPRE9,6409,6609,6809,7009,7209,7409,7609,7809,800
    CGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCGTCGACTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGCAGCTGAAATTCTGGTTACTGAATGTTCCGTCGACATCTAGAATCGGTGAWPRE9,8209,8409,8609,8809,9009,9209,9409,960
    TTTTAAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAAAATTTTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTTCTGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCA3'\LTR\SINHIV R9,98010,00010,02010,04010,06010,08010,10010,12010,140
    AGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTACGTATAGTAGTTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATTGCAGCTTCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATGCATATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGASnaBI3'\LTR\SINHIV U5SV40 p...ignalSV40 p...ignal10,16010,18010,20010,22010,24010,26010,28010,30010,320
    TATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCTCTAGCTATCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGAGATCGATAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTSV40 polyadenylation signalSV40 poly(A) signal10,34010,36010,38010,40010,42010,44010,46010,48010,500
    TTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGGACGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCCTGCATGGGTTAAGCGGGATATCACTCAGCATAATGCGCGCGAGTGACCGGCAGCAAAATGTTGCAGSfiIAvrIISV40 origin of replicationT7T7M13-fwd10,52010,54010,56010,58010,60010,62010,64010,660
    GTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCAFspILacZ alphaF1\ORIF1 oriF1 ori10,68010,70010,72010,74010,76010,78010,80010,82010,840
    GGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATCCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAF1\ORIF1 oriF1 ori10,86010,88010,90010,92010,94010,96010,98011,00011,020
    TAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAF1\ORIF1 oriF1 ori11,04011,06011,08011,10011,12011,14011,16011,18011,200
    TAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAATCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTSspIBspHICspCIF1 oriF1\ORI11,22011,24011,26011,28011,30011,32011,34011,360
    TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCSspIAmpR11,38011,40011,42011,44011,46011,48011,50011,52011,540
    GTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCACATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTScaIAmpRAmpR11,56011,58011,60011,62011,64011,66011,68011,70011,720
    TCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTAAmpRAmpR11,74011,76011,78011,80011,82011,84011,86011,88011,900
    GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTCTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGAFspIAmpRAmpR11,92011,94011,96011,98012,00012,02012,04012,060
    GGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAmpRAmpR12,08012,10012,12012,14012,16012,18012,20012,22012,240
    TTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGABspHI12,26012,28012,30012,32012,34012,36012,38012,40012,420
    TCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGG12,44012,46012,48012,50012,52012,54012,56012,58012,600
    ACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCC12,62012,64012,66012,680
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12697 bp9000100001100012000100020003000InsertBCL6Old BsmBI SiteF1 origagDelta GAGpsiAmpRAmpR2964-5085lac promoterLacO,M13-rev,T3 promoter,T3,5'LTR,HIV R,SphI,BaeIHIV U5,PSI,Major splice donorNruINotI,MfeIRREKflI,PpuMI,NdeI,NheI,BmtISFFV,EcoRV,TspMI,XmaI,SmaIPpuMI,AscIOld BsmBI SiteOld BsmBI Site,Gateway recomb1,attB1,BspDI,ClaI,BaeIWPREOld BsmBI Site3'\LTR\SIN,HIV RHIV U5,SnaBISV40 poly(A) signal,SV40 polyadenylation signalSV40 origin of replication,T7,T7,Old BsmBI Site,M13-fwd,SfiI,+1LacZ alpha,FspIF1\ORI,F1 oriCspCI,SspI,BspHISspIScaI,FspIBspHI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    12697