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LSM0251 (PB)EF1a-FKBP-tm-ADAR2dd-IRESv0-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggW286LSM250xz066220406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIHK284IL282SA280GI278EA276I174-1031: Region 1Q274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228174-1031: Region 2AmpLSM250xz0662180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaFspIWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170174-1031: Region 2AmpLSM250xz0662360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L174-1031: Region 2AmpLSM250xz0662540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaScaIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K174-1031: Region 2AmpLSM250xz0662720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M174-1031: Region 2AmpLSM250xz06628809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaCspCILSM250xz06621,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggAloIPsiILSM250xz06621,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattLSM250xz06621,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagFspILSM250xz06621,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataa5' PB ITR Longjt1009overlapLSM250xz06621,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttBsiWI5' PB ITR Long5' PB ITR shortLSM250xz06621,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccRsrIIcore insul5' PB ITR LongLSM250xz06622,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTASfiISpeISrfIcore insulattD Cp36Forward Targettarget ...primerLSM250xz06622,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAGGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAMfeIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1aLSM250xz06622,4602,4802,5002,5202,5402,5602,5802,6002,620
    GAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGAgeIEcoNIEF-1α promoterLSM250xz06622,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGAflIISacIIEF-1α promoterLSM250xz06622,8202,8402,8602,8802,9002,9202,9402,960
    GCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGBglIIEF-1α promoterLSM250xz06622,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTApaIFseISacIIPspOMIEF-1α promoterLSM250xz06623,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGBbvCIEcoNIBpu10IBstAPIEF-1α promoterLSM250xz06623,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAEF-1α promoterfrag1LSM250xz06623,5203,5403,5603,5803,6003,6203,6403,660
    CAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAATCTAGAAGCCACCatggcgctccctgtcaccgcactgcttcttccgctggcactgctgctgcacgctgcacggcctggatccATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGtaccgcgagggacagtggcgtgacgaagaaggcgaccgtgacgacgacgtgcgacgtgccggacctaggTACCCTCACGTCCACCTTTGGTAGAGGGGTCCTCTGCCCGXbaIBsmBIEsp3IMA2LP4VT6AL8LL10PL12AL14LL16HA18AR20PG22SM24GV26QV28ET30IS32PG34DG363742-5640G1S23xz123CD8α signal peptideEF-1α promoterfrag1FKBPfrom AV132RF from EF-1ahomologLSM250xz06623,6803,7003,7203,7403,7603,7803,8003,8203,840
    GCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAATTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGCGTGGAAGGGGTTCGCGCCGGTCTGGACGCACCACGTGATGTGGCCCTACGAACTTCTACCTTTCTTTAAACTAAGGAGGGCCCTGTCTTTGTTCGGGAAATTCAAATACGATCCGTTCGTCCTCCACTAGGCTCCGACCCTTCTTCCCCAACGGGTCTACTCACACCCAGTCTCRT38FP40KR42GQ44TC46VV48HY50TG52ML54ED56GK58KF60DS62SR64DR66NK68PF70KF72ML74GK76QE78VI80RG82WE84EG86VA88QM90SV92GQ94R3742-56403xz123FKBPfrom AV1323,8603,8803,9003,9203,9403,9603,9804,0004,020
    AGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTGGAAggcgccgggacaggagggtctgggggtggcactggcggatctggaggagactacaaggacgatgacTCGGTTTGACTGATATAGAGGTCTAATACGGATACCACGGTGACCCGTGGGTCCGTAGTAGGGTGGTGTACGGTGAGAGCAGAAGCTACACCTCGAAGATTTTGACCTTccgcggccctgtcctcccagacccccaccgtgaccgcctagacctcctctgatgttcctgctactgA96KL98TI100SP102DY104AY106GA108TG110HP112GI114IP116PH118AT120LV122FD124VE126LL128KL130EG132AG134TG136GS138GG140GT142GG144SG146GD148YK150DD152D3742-56403xz123FKBPfrom AV13245-ECLfrom XG 2054,0404,0604,0804,1004,1204,1404,1604,1804,200
    gacaacggcggatcgggggggtctggtggtagtggcggttcggggggaggtaccggtctggtcgtggttgctggagtcctgttttgttatggcttgctagtgacagtggctctttgtgttgaaaattcCGGGTCAGGCGGTTCCGGCAGCGGGGCGGGTAGCGGGTCCAGTGGGTctgttgccgcctagcccccccagaccaccatcaccgccaagcccccctccatggccagaccagcaccaacgacctcaggacaaaacaataccgaacgatcactgtcaccgagaaacacaacttttaagGCCCAGTCCGCCAAGGCCGTCGCCCCGCCCATCGCCCAGGTCACCCAAgeIKpnIPsrIAcc65ID154NG156GS158GG160SG162GS164GG166SG168GG170TG172LV174VV176AG178VL180FC182YG184LL186VT188VA190LC192VE194NS196GS198GG200SG202SG204AG206SG208SS210G3742-56403xz123from XG 205CD28-TMD45-ECLGS+XTEN linker4,2204,2404,2604,2804,3004,3204,3404,360
    CAGAAACACCCGGCACGAGTGAGTCAGCAACGCCAGAGAGTgctagcGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACGTCTTTGTGGGCCGTGCTCACTCAGTCGTTGCGGTCTCTCAcgatcgCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGBmtINheIBstEIIS212ET214PG216TS218ES220AT222PE224SA226SG228AL230HL232DQ234TP236SR238QP240IP242SE244GL246QL248HL250PQ252VL254AD256AV258SR260LV262LG264KF266GD268LT2703742-56404hADAR2 cat domain3xz123GS+XTEN linkerLSM250xz0664,3804,4004,4204,4404,4604,4804,5004,5204,540
    CGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATDN272FS274SP276HA278RR280KV282LA284GV286VM288TT290GT292DV294KD296AK298VI300SV302ST304GT306KC308IN310GE312YM314SD316RG318LA320LN322DC324HA326EI3283742-56404hADAR2 cat domainLSM250xz0664,5604,5804,6004,6204,6404,6604,6804,7004,720
    ATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTBclIBsrGIIS330RR332SL334LR336FL338YT340QL342EL344YL346NN348KD350DQ352KR354SI356FQ358KS360ER362GG364FR366LK368EN370VQ372FH374LY376IS378TS380PC382GD384AR3863742-56404hADAR2 cat domainLSM250xz0664,7404,7604,7804,8004,8204,8404,8604,8804,900
    TCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTAIF388SP390HE392PI394LE396EP398AD400RH402PN404RK406AR408GQ410LR412TK414IE416SG418QG420TI422PV424RS426NA428SI430QA432WD434GV436LQ438GE440RL442LT444M3742-56404hADAR2 cat domainLSM250xz0664,9204,9404,9604,9805,0005,0205,0405,060
    GTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGABlpIPpuMIS446CS448DK450IA452RW454NV456VG458IQ460GS462LL464SI466FV468EP470IY472FS474SI476IL478GS480LY482HG484DH486LS488RA490MY492QR494IS496NI498ED500LP502P3742-56404hADAR2 cat domainhomologLSM250xz0665,0805,1005,1205,1405,1605,1805,2005,2205,240
    CTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCMluIHincIIL504YT506LN508KP510LL512SG514IS516NA518EA520RQ522PG524KA526PN528FS530VN532WT534VG536DS538AI540EV542IN544AT546TG548KD550EL552GR554AS556RL558CK560H3742-56404hADAR2 cat domainLSM250xz0665,2605,2805,3005,3205,3405,3605,3805,4005,420
    CGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTA562LY564CR566WM568RV570HG572KV574PS576HL578LR580SK582IT584KP586NV588YH590ES592KL594AA596KE598YQ600AA602KA604RL606FT608AF610IK612AG614LG616AW618VE6203742-56404hADAR2 cat domainLSM250xz0665,4405,4605,4805,5005,5205,5405,5605,5805,600
    GAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGApaIPspOMIKP622TE624QD626QF628SL630TP632*3742-56404EMCV IRES SitehADAR2 cat domainPCRhomologLSM250xz0665,6205,6405,6605,6805,7005,7205,7405,760
    TGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGAvrIIHindIII4EMCV IRES SiteLSM250xz0665,7805,8005,8205,8405,8605,8805,9005,9205,940
    CAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCAGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTKpnIPmlIPflMIAcc65I4EMCV IRES SiteLSM250xz0665,9605,9806,0006,0206,0406,0606,0806,1006,120
    CATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCBmgBIBsiWIMT2EY4KP6TV8RL10AT12RD14DV16PR18AV20RT22L6231-76044EMCV IRES Site1PuroRoverlapLSM250xz0666,1406,1606,1806,2006,2206,2406,2606,2806,300
    CCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCARsrIIBstEIIA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL52FL54TR56VG58LD60IG62KV64WV66AD68DG70AA72VA74VW76TT78PE80SV826231-76041PuroRPR_S030PR_S029LSM250xz0666,3206,3406,3606,3806,4006,4206,4406,460
    CGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGEA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME110GL112LA114PH116RP118KE120PA122WF124LA126TV128GV130SP132DH134QG136KG138LG1406231-76041PuroRLSM250xz0666,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACBsaIEagISexAIBstAPISA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL168PF170YE172RL174GF176TV178TA180DV182EV184PE186GP188RT190WC192MT194RK196PG1986231-76041PuroRLSM250xz0666,6606,6806,7006,7206,7406,7606,7806,8006,820
    CCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaaGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttSapIBmgBIBspQIBsrGIAA200TN202FS204LL206KQ208AG210DV212EE214NP216GP218GS220MV222SK224GE226EL228IK230EN232MH234MK236LY238ME240GT242VD244NH246HF248KC250TS252EG254EG256K6231-76041mtagBFP2P2AhomologoushomologLSM250xz0666,8406,8606,8806,9006,9206,9406,9606,9807,000
    gccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctcBsu36IP258YE260GT262QT264MR266IK268VV270EG272GP274LP276FA278FD280IL282AT284SF286LY288GS290KT292FI294NH296TQ298GI300PD302FF304KQ306SF308PE310GF312TW314E6231-76041mtagBFP2LSM250xz0667,0207,0407,0607,0807,1007,1207,1407,160
    agagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgtctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcAloIR316VT318TY320ED322GG324VL326TA328TQ330DT332SL334QD336GC338LI340YN342VK344IR346GV348NF350TS352NG354PV356MQ358KK360TL362GW364EA366FT368ET370LY372P6231-76041mtagBFP2LSM250xz0667,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgagactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctAccIA374DG376GL378EG380RN382DM384AL386KL388VG390GS392HL394IA396NA398KT400TY402RS404KK406PA408KN410LK412MP414GV416YY418VD420YR422LE424RI426KE428AN430NE4326231-76041mtagBFP2LSM250xz0667,3607,3807,4007,4207,4407,4607,4807,5007,520
    aacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCCTCGAGCTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGGAGCTCGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGBclIPacIAlwNIEcoRIPspXITY434VE436QH438EV440AV442AR444YC446DL448PS450KL452GH454KL456N*4586231-76041mtagBFP2bGH poly(A) signalhomologLSM250xz0667,5407,5607,5807,6007,6207,6407,6607,6807,700
    TGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatcACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagSphIEcoRV1bGH poly(A) signalLSM250xz0667,7207,7407,7607,7807,8007,8207,8407,860
    tataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgc13' PB ITR longLSM250xz0667,8807,9007,9207,9407,9607,9808,0008,0208,040
    cctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgaNsiISphI13' PB ITR long3' PB ITR shortjt1010LSM250xz0668,0608,0808,1008,1208,1408,1608,1808,2008,220
    cagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgccgtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcgg1LSM250xz0668,2408,2608,2808,3008,3208,3408,3608,3808,400
    tttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataa1LSM250xz0668,4208,4408,4608,4808,5008,5208,5408,560
    acgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcgg1LSM250xz0668,5808,6008,6208,6408,6608,6808,7008,7208,740
    tggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccSapIBspQI1LSM250xz0668,7608,7808,8008,8208,8408,8608,8808,9008,920
    tcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcaagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgt1LSM250xz06628,9408,9608,9809,0009,0209,0409,0609,0809,100
    tcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgcagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgLSM250xz06629,1209,1409,1609,1809,2009,2209,2409,260
    tgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctAlwNILSM250xz06629,2809,3009,3209,3409,3609,3809,4009,4209,440
    ggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaaccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttLSM250xz06629,4609,4809,5009,5209,5409,5609,5809,6009,620
    gcagcagattacgcgcagaaaaaaaggatctcaacgtcgtctaatgcgcgtctttttttcctagagttLSM250xz06629,640
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9659 bp70007500800085009000500100015002000LSM250xz0661Forward Targettarget forward primer3' PB ITR shortjt1010Amp2174-1031: Region 1174-1031: Region 26231-7604XZp112.2 (DNA)EK-ECLF (DNA)XZp018.4 (DNA)XZp044.4 (DNA)oLSM0085 (DNA)pUC_oriR_Rv2 (DNA)oLSM-pUC_ori_F (DNA)oLSM-pUC_ori_R (DNA)pUC_oriR_Fv2 (DNA)AhdI,BsaIFspIScaICspCIPsiIAloIFspIoverlapjt10095' PB ITR short,BsiWI5' PB ITR Longcore insul,RsrIISrfISfiISpeImtagBFP2AloIAccIXZp044.5 (DNA),AlwNI,PacIhomolog,EcoRI,PspXI,BclIbGH poly(A) signaloLSM0086 (DNA)SphI,EcoRV3' PB ITR longNsiISphIBspQISapIAlwNI

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    9659

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