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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggW28620406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIHK284IL282SA280GI278EA276I174-1031: Region 1Q274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228174-1031: Region 2Amp180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170174-1031: Region 2Amp360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L174-1031: Region 2Amp540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaScaIXmnIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K174-1031: Region 2Amp720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M174-1031: Region 2Amp8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaCspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcatt1,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcag1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataa5' PB ITR Longjt10091,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttBsiWI5' PB ITR Long5' PB ITR short1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccRsrIIcore insul5' PB ITR Long2,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTASfiISpeISrfIcore insulattD Cp36Forward Targettarget ...primer2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAGGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAABsmIMfeIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1a2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGAgeIEF-1α promoter2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGXmnIAflIISacIIEF-1α promoter2,8202,8402,8602,8802,9002,9202,9402,960
    GCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGBglIIEF-1α promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTFseIPciISacIIEF-1α promoter3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGBbvCIBpu10IBstAPIEF-1α promoter3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAEF-1α promoterfrag13,5203,5403,5603,5803,6003,6203,6403,660
    CAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAATCTAGAAGCCACCatgggtCTAGTAGATATAGAAGATGAAGAAAACATGAGTTCCAGCAGCACTGATGTGAAGGAAAACCGCAATCTGGACAACGTGTCCCCCAAGGATGGCAGCACACCTGGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGtacccaGATCATCTATATCTTCTACTTCTTTTGTACTCAAGGTCGTCGTGACTACACTTCCTTTTGGCGTTAGACCTGTTGCACAGGGGGTTCCTACCGTCGTGTGGACAhdIAleIXbaIM1G1LV2DI4ED6EE8NM10SS12SS14TD16VK18EN20RN22LD24NV26SP28KD30GS32TP343748-5859ADAR2 cdsEF-1α promoterfrag1RF from EF-1ahomolog3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GGCCTGGCGAGGGCTCTCAGCTCTCCAATGGGGGTGGTGGTGGCCCCGGCAGAAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAAAGGAGGAAAACACCAGGGCCCGTCCTCCCCAAGAACGCCCTGATGCAGCTGAATGAGATCAAGCCTGGCCGGACCGCTCCCGAGAGTCGAGAGGTTACCCCCACCACCACCGGGGCCGTCTTTCGCCGGGGACCTCCTCCCGTCGTTACCGGTGAGGTTCATGGCGGACTTCTTTTCCTCCTTTTGTGGTCCCGGGCAGGAGGGGTTCTTGCGGGACTACGTCGACTTACTCTAGTTCGGACCCspCIGP36GE38GS40QL42SN44GG46GG48GP50GR52KR54PL56EE58GS60NG62HS64KY66RL68KK70RR72KT74PG76PV78LP80KN82AL84MQ86LN88EI90KP92G3748-5859ADAR2 cds3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TTTGCAGTACACACTCCTGTCCCAGACTGGGCCCGTGCACGCGCCTTTGTTTGTCATGTCTGTGGAGGTGAATGGCCAGGTTTTTGAGGGCTCTGGTCCCACAAAGAAAAAGGCAAAACTCCATGCTGCTGAGAAGGCCTTGAGGTCTTTCGTTCAGTTTCCTAATGCCTCTGAGAAACGTCATGTGTGAGGACAGGGTCTGACCCGGGCACGTGCGCGGAAACAAACAGTACAGACACCTCCACTTACCGGTCCAAAAACTCCCGAGACCAGGGTGTTTCTTTTTCCGTTTTGAGGTACGACGACTCTTCCGGAACTCCAGAAAGCAAGTCAAAGGATTACGGAGACTCL94QY96TL98LS100QT102GP104VH106AP108LF110VM112SV114EV116NG118QV120FE122GS124GP126TK128KK130AK132LH134AA136EK138AL140RS142FV144QF146PN148AS150E3748-5859ADAR2 cds4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCCCACCTGGCCATGGGGAGGACCCTGTCTGTCAACACGGACTTCACATCTGACCAGGCCGACTTCCCTGACACGCTCTTCAATGGTTTTGAAACTCCTGACAAGGCGGAGCCTCCCTTTTACGTGGGCTCCAATGGGGATGACTCCTTCAGTTCCAGCGGGGACCTCAGCTTGTCGGGTGGACCGGTACCCCTCCTGGGACAGACAGTTGTGCCTGAAGTGTAGACTGGTCCGGCTGAAGGGACTGTGCGAGAAGTTACCAAAACTTTGAGGACTGTTCCGCCTCGGAGGGAAAATGCACCCGAGGTTACCCCTACTGAGGAAGTCAAGGTCGCCCCTGGAGTCGAACASfiIBbvCIBpu10IHincIIA152HL154AM156GR158TL160SV162NT164DF166TS168DQ170AD172FP174DT176LF178NG180FE182TP184DK186AE188PP190FY192VG194SN196GD198DS200FS202SS204GD206LS208L3748-5859ADAR2 cds4,2204,2404,2604,2804,3004,3204,3404,360
    CTGCTTCCCCGGTGCCTGCCAGCCTAGCCCAGCCTCCTCTCCCTGTCTTACCACCATTCCCACCCCCGAGTGGGAAGAATCCCGTGATGATCTTGAACGAACTGCGCCCAGGACTCAAGTATGACTTCCTCTCCGAGAGCGGGGAGAGCCATGCCAAGAGCTTCGTCATGTCTGTGACGAAGGGGCCACGGACGGTCGGATCGGGTCGGAGGAGAGGGACAGAATGGTGGTAAGGGTGGGGGCTCACCCTTCTTAGGGCACTACTAGAACTTGCTTGACGCGGGTCCTGAGTTCATACTGAAGGAGAGGCTCTCGCCCCTCTCGGTACGGTTCTCGAAGCAGTACAGACAS210AS212PV214PA216SL218AQ220PP222LP224VL226PP228FP230PP232SG234KN236PV238MI240LN242EL244RP246GL248KY250DF252LS254ES256GE258SH260AK262SF264VM266SV2683748-5859ADAR2 cds4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GGTCGTGGATGGTCAGTTCTTTGAAGGCTCGGGGAGAAACAAGAAGCTTGCCAAGGCCCGGGCTGCGCAGTCTGCCCTGGCCGCCATTTTTAACTTGCACTTGGATCAGACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACCCAGCACCTACCAGTCAAGAAACTTCCGAGCCCCTCTTTGTTCTTCGAACGGTTCCGGGCCCGACGCGTCAGACGGGACCGGCGGTAAAAATTGAACGTGAACCTAGTCTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGBbsISrfIHindIIIVV270DG272QF274FE276GS278GR280NK282KL284AK286AR288AA290QS292AL294AA296IF298NL300HL302DQ304TP306SR308QP310IP312SE314GL316QL318HL320PQ322VL324AD3263748-5859ADAR2 cdsADAR2 DD4,5604,5804,6004,6204,6404,6604,6804,7004,720
    GCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGACGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTBstEIIAV328SR330LV332LG334KF336GD338LT340DN342FS344SP346HA348RR350KV352LA354GV356VM358TT360GT362DV364KD366AK368VI370SV372ST374GT376KC378IN380GE382YM3843748-5859ADAR2 cdsADAR2 DD4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAABclISD386RG388LA390LN392DC394HA396EI398IS400RR402SL404LR406FL408YT410QL412EL414YL416NN418KD420DQ422KR424SI426FQ428KS430ER432GG434FR436LK438EN440VQ442F3748-5859ADAR2 cdsADAR2 DD4,9204,9404,9604,9805,0005,0205,0405,060
    TCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTGAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCACTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTBsrGIH444LY446IS448TS450PC452GD454AR456IF458SP460HE462PI464LE466EP468AD470RH472PN474RK476AR478GQ480LR482TK484IE486SG488EG490TI492PV494RS496NA498SI500Q3748-5859ADAR2 cdsADAR2 DD5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ACGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGTGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCBlpIBamHIT502WD504GV506LQ508GE510RL512LT514MS516CS518DK520IA522RW524NV526VG528IQ530GS532LL534SI536FV538EP540IY542FS544SI546IL548GS550LY552HG554DH556LS558R3748-5859ADAR2 cdsADAR2 DD5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCHincIIA560MY562QR564IS566NI568ED570LP572PL574YT576LN578KP580LL582SG584IS586NA588EA590RQ592PG594KA596PN598FS600VN602WT604VG606DS608AI610EV612IN614AT616TG6183748-5859ADAR2 cdsADAR2 DD5,4405,4605,4805,5005,5205,5405,5605,5805,600
    GAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACMluIKD620EL622GR624AS626RL628CK630HA632LY634CR636WM638RV640HG642KV644PS646HL648LR650SK652IT654KP656NV658YH660ES662KL664AA666KE668YQ670AA672KA674RL6763748-5859ADAR2 cdsADAR2 DD5,6205,6405,6605,6805,7005,7205,7405,760
    TTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCACTAGTCGCTAGCGAAGGTCGGGGGAGTTTGCTTACCTGTGGCGATGTCGAGGAGAATCCAGGGCCCAAGCTTATGACCGAGTACAAGCCCACGGTGCAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGTGATCAGCGATCGCTTCCAGCCCCCTCAAACGAATGGACACCGCTACAGCTCCTCTTAGGTCCCGGGTTCGAATACTGGCTCATGTTCGGGTGCCACGBmtINheISpeIHindIIIAS2EG4RG6SL8LT10CG12DV14EE16NP18GP20KL22MT24EY26KP28TV305860-7299FT678AF680IK682AG684LG686AW688VE690KP692TE694QD696QF698SL700TP702LV7043748-5859ADAR2 cdsADAR2 DDT2APuroRfrag35,7805,8005,8205,8405,8605,8805,9005,9205,940
    GCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGBsiWIRsrIIBstEIIRL32AT34RD36DV38PR40AV42RT44LA46AA48FA50DY52PA54TR56HT58VD60PD62RH64IE66RV68TE70LQ72EL74FL76TR78VG80LD82IG84KV86WV88A5860-7299PuroRfrag3PR_S030PR_S0295,9605,9806,0006,0206,0406,0606,0806,1006,120
    GGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACD90DG92AA94VA96VW98TT100PE102SV104EA106GA108VF110AE112IG114PR116MA118EL120SG122SR124LA126AQ128QQ130ME132GL134LA136PH138RP140KE142PA144WF146L5860-7299PuroRfrag36,1406,1606,1806,2006,2206,2406,2606,2806,300
    GCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCBsaIEagIA148TV150GV152SP154DH156QG158KG160LG162SA164VV166LP168GV170EA172AE174RA176GV178PA180FL182ET184SA186PR188NL190PF192YE194RL196GF198TV200TA202DV204E5860-7299PuroRfrag36,3206,3406,3606,3806,4006,4206,4406,460
    TGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcacACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtgBamHIBmgBIBsrGIPaqCISexAIBstAPIV206PE208GP210RT212WC214MT216RK218PG220AA222TN224FS226LL228KQ230AG232DV234EE236NP238GP240GS242MV244SK246GE248EL250IK252EN254MH256MK258LY260ME262GT2645860-7299frag3frag4mtagBFP2P2APuroRhomologoushomolog6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatcgcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtagVD266NH268HF270KC272TS274EG276EG278KP280YE282GT284QT286MR288IK290VV292EG294GP296LP298FA300FD302IL304AT306SF308LY310GS312KT314FI316NH318TQ320GI3225860-7299frag4mtagBFP26,6606,6806,7006,7206,7406,7606,7806,8006,820
    cccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagagggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtctBsu36IPD324FF326KQ328SF330PE332GF334TW336ER338VT340TY342ED344GG346VL348TA350TQ352DT354SL356QD358GC360LI362YN364VK366IR368GV370NF372TS374NG376PV378MQ3805860-7299frag4mtagBFP26,8406,8606,8806,9006,9206,9406,9606,9807,000
    agaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctatcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatAccIKK382TL384GW386EA388FT390ET392LY394PA396DG398GL400EG402RN404DM406AL408KL410VG412GS414HL416IA418NA420KT422TY424RS426KK428PA430KN432LK434MP436GV438Y5860-7299frag4mtagBFP27,0207,0407,0607,0807,1007,1207,1407,160
    ctatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCCTCGAGCTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGGAGCTCGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAABclIPacIAlwNIEcoRIPspXIY440VD442YR444LE446RI448KE450AN452NE454TY456VE458QH460EV462AV464AR466YC468DL470PS472KL474GH476KL478N*4805860-7299frag4mtagBFP2homologbGH ...nal7,1807,2007,2207,2407,2607,2807,3007,3207,340
    GCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTbGH poly(A) signal7,3607,3807,4007,4207,4407,4607,4807,5007,520
    TAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccaBbsISphIEcoRV3' PB ITR longbGH po...ignal7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttgcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaaBsmINsiISphI3' PB ITR long3' PB ITR short7,7207,7407,7607,7807,8007,8207,8407,860
    aatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttjt10107,8807,9007,9207,9407,9607,9808,0008,0208,040
    cgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacgg8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctccacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgag8,2408,2608,2808,3008,3208,3408,3608,3808,400
    acaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcat8,4208,4408,4608,4808,5008,5208,5408,560
    ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggcaacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccgPciI8,5808,6008,6208,6408,6608,6808,7008,7208,740
    cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacagg8,7608,7808,8008,8208,8408,8608,8808,9008,920
    gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgactcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctga8,9408,9608,9809,0009,0209,0409,0609,0809,100
    tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatcatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactagAlwNI9,1209,1409,1609,1809,2009,2209,2409,260
    cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagtt9,2809,3009,3209,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    9354 bp70007500800085009000500100015002000core insul5' PB ITR shortjt1010Amp174-1031: Region 1174-1031: Region 25860-7299oLSM0084 (DNA)YH071-testF (DNA)XZp340.1 (DNA)oLSM0270 (DNA)oLSM0667 (DNA)XZp044.4 (DNA)XZp044.5 (DNA)AhdIBsaIScaIXmnICspCIPsiIjt1009BsiWI5' PB ITR LongRsrIISrfIAccIAlwNIhomolog,PacI,EcoRI,PspXI,BclIbGH poly(A) signalBbsISphIEcoRV3' PB ITR longBsmINsiISphI3' PB ITR shortPciIAlwNI

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    9354

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