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XZ030 CMV-TO-AVPR2-ADAR2(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcBsaBIBsaBIBsmBIEsp3ISgrDICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatatgctcatggcctctaccacaagcgcCgtgcccggttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctatacgagtaccggagatggtgttcgcgGcacgggccPmeIAflIIHindIIIMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28LM30AS32TT34SA36VP38G760-3270AVPR2 (Tango)Signal/FlagCMV promot...th TetOs)homologhomolog720740760780800820840860
    acatccatctcttccaagtcttccctccaacagctcacaggagaggcctctggatacccgggacccacttcttgcaagagcggagctcgccttgctctccatcgttttcgttgccgtcgcgttgagcaatggtctggtgctggccgccctggcgcgacgaggccgccgaggccactgtaggtagagaaggttcagaagggaggttgtcgagtgtcctctccggagacctatgggccctgggtgaagaacgttctcgcctcgagcggaacgagaggtagcaaaagcaacggcagcgcaactcgttaccagaccacgaccggcgggaccgcgctgctccggcggctccggtgBplIBplISfiISmaIStuIXmaITspMIH40PS42LP44SL46PS48NS50SQ52ER54PL56DT58RD60PL62LA64RA66EL68AL70LS72IV74FV76AV78AL80SN82GL84VL86AA88LA90RR92GR94RG96H760-3270AVPR2 (Tango)8809009209409609801,0001,0201,040
    tgggcgcctattcatgtctttatcggccatctctgtcttgcagacctggccgtggcgctgtttcaggtgctccctcaactggcttggaaagccaccgatagattccgggggccagacgcactgtgtcgggccgtgaaatacctgcagatggtgggaatgtatgccagttcatataacccgcggataagtacagaaatagccggtagagacagaacgtctggaccggcaccgcgacaaagtccacgagggagttgaccgaacctttcggtggctatctaaggcccccggtctgcgtgacacagcccggcactttatggacgtctaccacccttacatacggtcaagtatatKasINarISfoIPluTIW98AP100IH102VF104IG106HL108CL110AD112LA114VA116LF118QV120LP122QL124AW126KA128TD130RF132RG134PD136AL138CR140AV142KY144LQ146MV148GM150YA152SS154Y760-3270AVPR2 (Tango)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgatacttgctatgaccttggacagacacagggctatctgtcgccccatgctggcgtataggcacggttccggagcccattggaacaggccagttctggttgcgtgggctttttccctgcttctgagcttgccccagttgtttattttcgcacagagaaatgtggagggcggatcactatgaacgatactggaacctgtctgtgtcccgatagacagcggggtacgaccgcatatccgtgccaaggcctcgggtaaccttgtccggtcaagaccaacgcacccgaaaaagggacgaagactcgaacggggtcaacaaataaaagcgtgtctctttacacctcccgcctagM156IL158AM160TL162DR164HR166AI168CR170PM172LA174YR176HG178SG180AH182WN184RP186VL188VA190WA192FS194LL196LS198LP200QL202FI204FA206QR208NV210EG212GS214760-3270AVPR2 (Tango)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tggcgtgaccgattgctgggcttgcttcgctgagccatggggcagaaggacctacgtgacgtggattgctctcatggtgttcgttgctcccaccctggggatcgccgcatgccaggtcctgatattcagagaaatacacgcctccttggttcctgggccaagcgagcgccctggtaccgcactggctaacgacccgaacgaagcgactcggtaccccgtcttcctggatgcactgcacctaacgagagtaccacaagcaacgagggtgggacccctagcggcgtacggtccaggactataagtctctttatgtgcggaggaaccaaggacccggttcgctcgcgggaccaBlpIPasISphIBmgBIGV216TD218CW220AC222FA224EP226WG228RR230TY232VT234WI236AL238MV240FV242AP244TL246GI248AA250CQ252VL254IF256RE258IH260AS262LV264PG266PS268ER270PG272760-3270AVPR2 (Tango)1,4201,4401,4601,4801,5001,5201,5401,560
    ggaagaagacggggtcgaaggactggctctccaggcgaaggcgcccacgtatcagctgcggtagctaagaccgtacgcatgacactggtcatcgtcgtcgtttacgttctttgctgggccccgttttttttggtgcagctctgggcggcctgggaccccgaagcacccctggaagccttcttctgccccagcttcctgaccgagaggtccgcttccgcgggtgcatagtcgacgccatcgattctggcatgcgtactgtgaccagtagcagcagcaaatgcaagaaacgacccggggcaaaaaaaaccacgtcgagacccgccggaccctggggcttcgtggggaccttcApaIKasINarISfoIBsiWIPluTIPshAIPspOMIGR274RR276GR278RT280GS282PG284EG286AH288VS290AA292VA294KT296VR298MT300LV302IV304VV306YV308LC310WA312PF314FL316VQ318LW320AA322WD324PE326AP328LE330760-3270AVPR2 (Tango)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gtgctccattcgttctgctcatgcttctcgcttcactgaatagctgtaccaatccttggatttacgcctctttctcctccagtgttagctccgagctgcggagtcttctctgttgcgcccggggcaggacccccccaagcctgggtccacaggacgaatcctgcactaccgcctccacgaggtaagcaagacgagtacgaagagcgaagtgacttatcgacatggttaggaacctaaatgcggagaaagaggaggtcacaatcgaggctcgacgcctcagaagagacaacgcgggccccgtcctgggggggttcggacccaggtgtcctgcttaggacgtgatggcggagSmaIXmaIPflMITspMIGA332PF334VL336LM338LL340AS342LN344SC346TN348PW350IY352AS354FS356SS358VS360SE362LR364SL366LC368CA370RG372RT374PP376SL378GP380QD382ES384CT386TA388S760-3270AVPR2 (Tango)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cagttccctggccaaagacacctcttctatcgataccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTgtcaagggaccggtttctgtggagaagatagctatggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCAAgeIAgeIKpnIXcmIBstXIEcoNIAcc65IS390SL392AK394DT396SS398ID400TG402GR404TP406PS408LG410PQ412DE414SC416TT418AS420SS422LA424KD426TS428SG430TG432AL434HL436DQ438TP440SR442QP444IP446S760-3270AVPR2 (Tango)V2 tailhADAR2 cat domainhomologous1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGCTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACBstEIIE448GL450QL452HL454PQ456VL458AD460AV462SR464LV466LG468KF470GD472LT474DN476FS478SP480HA482RR484KV486LA488GV490VM492TT494GT496DV498KD500AK502VI504S760-3270hADAR2 cat domain2,1202,1402,1602,1802,2002,2202,2402,260
    TTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGBclIV506ST508GT510KC512IN514GE516YM518SD520RG522LA524LN526DC528HA530EI532IS534RR536SL538LR540FL542YT544QL546EL548YL550NN552KD554DQ556KR558SI560FQ562KS564760-3270hADAR2 cat domain2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCABcgIPfoIBsrGIER566GG568FR570LK572EN574VQ576FH578LY580IS582TS584PC586GD588AR590IF592SP594HE596PI598LE600EP602AD604RH606PN608RK610AR612GQ614LR616TK618IE620SG622760-3270hADAR2 cat domain2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTBlpITliIXhoIBamHIPaeR7IQG624TI626PV628RS630NA632SI634QA636WD638GV640LQ642GE644RL646LT648MS650CS652DK654IA656RW658NV660VG662IQ664GS666LL668SI670FV672EP674IY676FS678SI680760-3270hADAR2 cat domain2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCAIL682GS684LY686HG688DH690LS692RA694MY696QR698IS700NI702ED704LP706PL708YT710LN712KP714LL716SG718IS720NA722EA724RQ726PG728KA730PN732FS734VN736WT738V760-3270hADAR2 cat domain2,8202,8402,8602,8802,9002,9202,9402,960
    AGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGTCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCMluIG740DS742AI744EV746IN748AT750TG752KD754EL756GR758AS760RL762CK764HA766LY768CR770WM772RV774HG776KV778PS780HL782LR784SK786IT788KP790NV792YH794ES796K760-3270hADAR2 cat domain2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcApaITliIXhoIPspXIPaeR7IPspOMI*1L798AA800KE802YQ804AA806KA808RL810FT812AF814IK816AG818LG820AW822VE824KP826TE828QD830QF832SL834TP836*760-3270hADAR2 cat domainc-myc epitope3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagaBclIPmeIBGH polyadenylation sequencePolyhi...e tag3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctctcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagaSphIBGH polyadenylation sequenceBGH pA3,5203,5403,5603,5803,6003,6203,6403,660
    agggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggNaeINgoMIV3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagBarIXbaIPshAIFRTHygromycin3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaagBsmBIEsp3IHygromycin4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttgccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaaPvuIAsiSIEcoRIBstAPIDraIIIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    cggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtcgccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcagNdeIRsrIIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagcAjuIBstAPIDraIIIHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    agcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacatcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtSacIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgPfoIScaIHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    gccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacataBsmINaeIPsiINgoMIVSV40 pA5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaaBstZ17ISV40 pA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcgcgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgcSapIBspQI5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctPciIpUC ori5,6205,6405,6605,6805,7005,7205,7405,760
    caggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgapUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctcccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggapUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    aactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcttgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctagpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttgaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaapUC oriAmp...a)6,3206,3406,3606,3806,4006,4206,4406,460
    aatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtAhdIBsaIAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcacggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtAclIFspIAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaPvuIAmp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacAclIBcgIScaIXmnIAmp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    ttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaaaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttSspIAmp (bla)bla promoter7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcacgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtSgrDIbla promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    tagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWI7,5407,5607,5807,6007,6207,6407,6607,680
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7686 bp550060006500700050010001500AVPR2 (Tango)homologundesired cut site*760-3270EKbF (DNA)XZp025.2 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA)CMV,EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),EK_CMVFd (DNA),EKo272.1 (DNA),+2CMV promoter (with TetOs),A,EK_CMVFe (DNA),SnaBI,CspCITATA box,TetO,TetR binding sites,TetO,CMV-Forward Genewiz (DNA),+5homolog,Kozak,startt,Signal/Flag,CMVF1 (DNA),ToFlpF (DNA),+5CCp134 SignalSeq-R (DNA),XZp025.3 (DNA),StuI,TspMI,XmaISmaI,BplI,BplISfiI,KasI,NarI,SfoI,PluTIAVPR2-seq-F1 (DNA)BlpI,BmgBIPasI,SphIAVPR2-seq-F2 (DNA),KasI,NarI,SfoI,PluTIBsiWIPshAI,PspOMI,ApaIPflMITspMI,XmaI,SmaIXZ029-test (DNA),XZp025.4 (DNA)pUCoriR (DNA)pUC oriAhdIBsaIFspI,AclIAmp (bla),PvuIScaI,BcgI,AclI,XmnISspIbla promoter,FlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),+5

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    7686

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