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pX330A_hCas9_long_chimeric_gRNA_G2P
  • Sequence Map
    Editing disabled on read-only sequence.
    CCGGCCAGGCAAAAAAGAAAAAGtaagaattcCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTGGCCGGTCCGTTTTTTCTTTTTCattcttaagGATCTCGAGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAFseISacIEcoRIEco53kIpApX330A_screen_RhCas9(2)hCas9(3)20406080100120140160
    CTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGAgAATAGCAGGCATGCTGGGGAgcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTcTTATCGTCCGTACGACCCCTcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcNotISphIpA180200220240260280300320340
    cccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgCCTGCAGGaaagagcccacaacccctcacttggtgggccagtcctctgatagactgtgtcccctggatacccgtattctagagctagcgaattcgaatttaaatcggatccgcggccgcggggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacGGACGTCCtttctcgggtgttggggagtgaaccacccggtcaggagactatctgacacaggggacctatgggcataagatctcgatcgcttaagcttaaatttagcctaggcgccggcgcBmtINheINotISbfISmaISmaISrfISrfISwaIXbaIXmaIXmaIBamHIBstBIEcoRIPflFITspMITspMITth111IMSCVmu promoterMCSG2P_SbfI_F360380400420440460480500520
    aaggatctgcgatcgctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggattcctagacgctagcgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctMfeIPvuIAsiSIEF1 promoter540560580600620640660680700
    gaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcttggcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagagaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacCspCIBsu36IHindIIIEF1 promoterG2P_s1_F720740760780800820840860
    cctcctgaactgcgtccgccgtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatcggaggacttgacgcaggcggcagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctagEF1 promoter8809009209409609801,0001,0201,040
    caagctgtgaccggcgcctacgctagacgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcgttcgacactggccgcggatgcgatctgcggtggtacctctcgctgctctcgccggacgggcggtacctctagctcacggcgtagtggccgtgggacttgccgcacctcaagctcgaccacccgccgcctctcccgtgggggttcgtcccggcgtactggttgttctacttctcgSapIBspQIcopGFPEF1 promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    accaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtggtttccgcgggactggaagtcggggatggacgactcggtgcactacccgatgccgaagatggtgaagccgtggatggggtcgccgatgctcttggggaaggacgtgcggtagttgttgccgccgatgtggttgtgggcgtagctcttcatgctcctgccgccgcacgacgtgccopGFP1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcacactcgaagtcgatggcgatgctccggccggcgcactagccgctgaagttccaccacccgtggccgaaggggctcctgtcgcactagaagtggctgttctagtaggcgtcgttgcggtggcacctcgtggacgtggggtacccgctattgcacgaccacccgtcgaagcgggcgtgFseIPaqCIcopGFP1,4201,4401,4601,4801,5001,5201,5401,560
    cttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcgaagtcggacgcgctgccgccgatgatgtcgaagcaccacctgtcggtgtacgtgaagttctcgcggtaggtggggtcgtaggacgtcttgcccccggggtacaagcggaaggcggcgcacctcctcgacgtgtcgttgtggctcgacccgtagcacctcatggtcgtgcggaagApaIPspOMIcopGFP1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTatgaccgagtacaagcccacggtgcgcctcgttctgggggtagcggaagcggtctagggcgcgagtcagcaggttaagacggcacctgccgtggcggcctgggccgaggtggcctagagcgCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGAtactggctcatgttcgggtgccacgcggagcNruIcopGFPT2APuro1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgaggtgggcgctgctgcaggggtcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctPasIBsiWIPflFIRsrIIBstEIITth111IPuro1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggStuIPuro2,1202,1402,1602,1802,2002,2202,2402,260
    gtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgcagccgcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcPuro2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcGTTaactaAACTTGTTTATTGCAGttcctggcgcgtggaccacgtactgggcgttcgggccacggactttagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgCAAttgatTTGAACAAATAACGTCHpaISexAIHincIIPuroSV40 pA2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGAATTGACTCAAATGATGTCAATTAGTCTATCAGAAGCTATCTGGTCTCCCTTCCGGGCCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCTTAACTGAGTTTACTACAGTTAATCAGATAGTCTTCGATAGACCAGAGGGAAGGCCCGGBsmIPsiISV40 pAG2P_SbfI_R2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TGCAGGggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcACGTCCccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagSbfIM13 originF1 ori2,8202,8402,8602,8802,9002,9202,9402,960
    gctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgcccttcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaM13 originF1 ori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgPsiISspIM13 originF1 ori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcacaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtM1...n3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtact3,5203,5403,5603,5803,6003,6203,6403,660
    gacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccSspIAmp3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaaAmp3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccctcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggPvuIAmp4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccactaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaFspIAmp4,2204,2404,2604,2804,3004,3204,3404,360
    tctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcaccttcggcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgaAmp4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagctctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtcColE1 originAmp4,5604,5804,6004,6204,6404,6604,6804,7004,720
    accccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaColE1 origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgcColE1 origin4,9204,9404,9604,9805,0005,0205,0405,060
    gggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcColE1 origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtgagggcctatttcccatgattcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacactcccggataaagggtactaagPciIAflIIIColE1 origin335B_FhU65,2605,2805,3005,3205,3405,3605,3805,4005,420
    cttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaacNdeIhU6gRNA_seqhU6hU6(1)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    aaagtatttcgatttcttggctttatatatcttGTGGAAAGGACGAAACACCggGTCTTCgaGAAGACctgttttagagctaGAAAtagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcTTTTTTgttttagagctagaaatagcaagtttcataaagctaaagaaccgaaatatatagaaCACCTTTCCTGCTTTGTGGccCAGAAGctCTTCTGgacaaaatctcgatCTTTatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgAAAAAAcaaaatctcgatctttatcgttchU6hU6 p...oter5,6205,6405,6605,6805,7005,7205,7405,760
    ttaaaataaggctagtccgtTTTTagcgcgtgcgccaattctgcagacaaatggctctagaggtacccgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaatagtaacgccaatagggactttccattgacgtcaatgggtaattttattccgatcaggcaAAAAtcgcgcacgcggttaagacgtctgtttaccgagatctccatgggcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttatcattgcggttatccctgaaaggtaactgcagttacccaKpnIXbaIAcc65ICAG_enhancerpX335B_Rseq_pX330_R5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattGtgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacccctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaaCacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggNdeISnaBICAG_enhancer5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggctaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtccgccccgccccgccccgctccccgccccgccccgctccg6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggccctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgctgcgacggaagcggggcacggggcgaggcggcggcggagcgcggcgggcggggccg6,3206,3406,3606,3806,4006,4206,4406,460
    tctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcactttttttcaggttGGaccggtgccagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgactcgttctccattcccaaattccctaccaaccaaccaccccataattacaaattaatggacctcgtggacggactttagtgaaaaaaagtccaaCCtggccacggAgeIPaqCI6,4806,5006,5206,5406,5606,5806,6006,6206,640
    accATGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGtggTACCTGATATTCCTGGTGCTGCCTCTGATGTTCCTAGTACTATAACTAATGTTTCTGCTACTGCTATTCTACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGCTGTTCTTCATGTCGTAGCCGGACCTGTAGCCGTGGTTGAGACACCCGACCCBstXIhCas9(2)hCas9(3)3xFLAGhCas9hCas9(1)hCas9(2)hCas9(3)SV40 NLShCas9(2)hCas9(3)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    CCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAAGGCACTAGTGGCTGCTCATGTTCCACGGGTCGTTCTTTAAGTTCCACGACCCGTTGTGGCTGGCCGTGTCGTAGTTCTTCTTGGACTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTCCGGTGGGCCGACTTCTCTTGGCGGTCTTCTTCTATGTGGTCTGCCTTCTThCas9(2)hCas9(3)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCGGCCTAGACGATAGACGTTCTCTAGAAGTCGTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCTGACCTTCTCAGGAAGGACCACCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCGTTGTAGCACCTGCTCCACCGGATGGTGCTCTTCATGGGGTGGTAGBglIIhCas9(2)hCas9(3)7,0207,0407,0607,0807,1007,1207,1407,160
    TACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCATGGTGGACTCTTTCTTTGACCACCTGTCGTGGCTGTTCCGGCTGGACGCCGACTAGATAGACCGGGACCGGGTGTACTAGTTCAAGGCCCCGGTGAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCGCTGCACCTGTTCGACAAGTAGGTCGACCACGTCTGGATGTTGGhCas9(2)hCas9(3)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    AGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTTCGACAAGCTCCTTTTGGGGTAGTTGCGGTCGCCGCACCTGCGGTTCCGGTAGGACAGACGGTCTGACTCGTTCTCGTCTGCCGACCTTTTAGACTAGCGGGTCGACGGGCCGCTCTTCTTCTTACCGGACAAGCCTTTGGACTAACGGGACTCGGACCCGGACTGGGGGTTGAABstXIhCas9(2)hCas9(3)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    CAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGTTCTCGTTGAAGCTGGACCGGCTCCTACGGTTTGACGTCGACTCGTTCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGGGTCTAGCCGCTGGTCATGCGGCTGGACAAAGACCGGCGGTTCTTGGACAGGCTGCGGTAGGACGACTCGCTGTAGGACTCTCACTTGTGGhCas9(2)hCas9(3)7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCCTCTAGTGGTTCCGGGGGGACTCGCGGAGATACTAGTTCTCTATGCTGCTCGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCCGTCGTCGACGGACTCTTCATGTTTCTCTAAAAGAAGCTGGTCTCGTTCTTGCCGATGCGGCCGATGTAACTGCCGCCTCGGTCGGhCas9(2)hCas9(3)7,7207,7407,7607,7807,8007,8207,8407,860
    AGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGATCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTTTTCTACCTGCCGTGGCTCCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCCTTCGTCGCCTGGAAGCTGTTGCCGTCGTAGGGGGTGGTCTAGGTGGACCCTCTCGACGTGCGGTAAGACGCCGCCGTCCThCas9(2)hCas9(3)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    AGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCTCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAGCTCTTCTAGGACTGGAAGGCGTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCGTCTAAGCGGACCTACTGGTCTTTCTCGCTCCTTTGGTAGTGGGGGACCTTGAAGCTCCTTCACCACCTGTTCCCGApaIPspOMIhCas9(2)hCas9(3)8,0608,0808,1008,1208,1408,1608,1808,2008,220
    GCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCCGAAGGCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTCTTGGACGGGTTGCTCTTCCACGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCACATATTGCTCGACTGGTTTCACTTTATGCACTGGCTCCCTTACTCTTTCGGGCGGAAGGACTCGCCGCTCGBarIhCas9(2)hCas9(3)8,2408,2608,2808,3008,3208,3408,3608,3808,400
    AGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAATCTTTTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTGGCACTTCGTCGACTTTCTCCTGATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAGAGGCCGCACCTTCTAGCCAAGTTGCGGAGGGACCCGTGTATGGTGCTAGACGACTTTTAATAGTTPasIhCas9(2)hCas9(3)8,4208,4408,4608,4808,5008,5208,5408,560
    GGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCCCTGTTCCTGAAGGACCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTATAGCACGACTGGGACTGTGACAAACTCCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGGGTGGACAAGCTGCTGTTTCACTACTTCGTCGACTTCGCCGCCTCTATGTGGCCGACCCCGEcoRVhCas9(2)hCas9(3)8,5808,6008,6208,6408,6608,6808,7008,7208,740
    AGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATATCCGACTCGGCCTTCGACTAGTTGCCGTAGGCCCTGTTCGTCAGGCCGTTCTGTTAGGACCTAAAGGACTTCAGGCTGCCGAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTGTCGGACTGGAAATTTCTCCTGTAGGTCTTTCGGGTCCACAGGCCGGTCCCGCTATAjuIhCas9(2)hCas9(3)8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCGGACGTGCTCGTGTAACGGTTAGACCGGCCGTCGGGGCGGTAATTCTTCCCGTAGGACGTCTGTCACTTCCACCACCTGCTCGAGCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCACTAGCTTTACCGGTCTCTCTTGGTCTGGTGGGTCTTCCCTGTCTTCTTGTCSacIEco53kIhCas9(2)hCas9(3)8,9408,9608,9809,0009,0209,0409,0609,0809,100
    CCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACGGCGCTCTCTTACTTCGCCTAGCTTCTCCCGTAGTTTCTCGACCCGTCGGTCTAGGACTTTCTTGTGGGGCACCTTTTGTGGGTCGACGTCTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCCCTATACATGCACCTGGTCCTTGACCTGTAGTTGGCCGACAGGCTGhCas9(2)hCas9(3)9,1209,1409,1609,1809,2009,2209,2409,260
    TACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCATGCTACACCTGGTATAGCACGGAGTCTCGAAAGACTTCCTGCTGAGGTAGCTGTTGTTCCACGACTGGTCTTCGCTGTTCTTGGCCCCGTTCTCGCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTACTTCTTGATGACCGCCGTCGACGACTTGCGGTTCGACTAATGGGhCas9(2)hCas9(3)9,2809,3009,3209,3409,3609,3809,4009,4209,440
    AGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGATCTCTTTCAAGCTGTTAGACTGGTTCCGGCTCTCTCCGCCGGACTCGCTTGACCTATTCCGGCCGAAGTAGTTCTCTGTCGACCACCTTTGGGCCGTCTAGTGTTTCGTGCACCGTGTCTAGGACCTGAGGGCCTACTTGTGATTCATGCTGCTCTTACTGTTCGACTAGGCCCTCspCIhCas9(2)hCas9(3)9,4609,4809,5009,5209,5409,5609,5809,6009,620
    AGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACTCACTTTCACTAGTGGGACTTCAGGTTCGACCACAGGCTAAAGGCCTTCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTGATGGTGGTGCGGGTGCTGCGGATGGACTTGCGGCAGCACCCTTGGCGGGACTAGTTTTTCATGGGATTCGACCTTTCGCTCAAGCACATGhCas9(2)hCas9(3)9,6409,6609,6809,7009,7209,7409,7609,7809,800
    GGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGCTGATGTTCCACATGCTGCACGCCTTCTACTAGCGGTTCTCGCTCGTCCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCGTTGTAGTACTTGAAAAAGTTCTGGCTCTAATGGGACCGGTTGCCGCTCTAGGCCTTCGCCGGAGACTAGCTCTGTTTGCCGCTTTAjuIhCas9(2)hCas9(3)9,8209,8409,8609,8809,9009,9209,9409,960
    CCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGAGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAACGGTGGCACGCCTTTCACGACTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCACGTCTGTCCGCCGAAGTCGTTTCTCAGATAGGACGGGTTCTCCTTGTCGCTATTCGACTAGCGGTCTTTCTTCCTGACCCTKflISphIhCas9(2)hCas9(3)9,98010,00010,02010,04010,06010,08010,10010,12010,140
    CCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGGATAAGACACGACCACCACCGGTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACACTTTCTCGACGACCCCTAGTGGTAGTACCTTTCTTCGTCGAAGCTCTTCTTAGGGTAGCTGAAAGACCTTCGGTTCBarIBsaBIhCas9(2)hCas9(3)10,16010,18010,20010,22010,24010,26010,28010,30010,320
    GGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGACCGATGTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTCATGAGGGACAAGCTCGACCTTTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGACGTCTTCCCTTTGCTTGACCGGGACGGGAGGTTTATACACTTGAAGGACATGGACCGGTCGGTGATACTCTBsmIhCas9(2)hCas9(3)10,34010,36010,38010,40010,42010,44010,46010,48010,500
    AGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATTCGACTTCCCGAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCTTGTCGTGTTCGTGATGGACCTGCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCACTAGGACCGGCTGCGATTAGACCTGTTTCACGACAGGCGGATGTTGTTCGTGGCCCTATTCGGGTAqPCR_FhCas9(2)hCas9(3)10,52010,54010,56010,58010,60010,62010,64010,660
    CAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGTCTCTCGTCCGGCTCTTATAGTAGGTGGACAAATGGGACTGGTTAGACCCTCGGGGACGGCGGAAGTTCATGAAACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTTCTCCACGACCTGCGGTGGGACTAGGTGGTCTCGTAGTGGCCGGACATGCTCTGTGCCTAGqPCR_RhCas9(2)hCas9(3)10,68010,70010,72010,74010,76010,78010,80010,82010,840
    GACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCTGGACAGAGTCGACCCTCCGCTGTTTTCCGGCCGCCGGTGCTTTTTCChCas9(2)hCas9(3)10,86010,880
    • Plasmid
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    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10899 bp800085009000950010000105005001000150020002500SV40 pAG2P_s1_FqPCR_RhCas9(2)hCas9(3)seq_plas_sgRNAs_r (DNA)seq_plas_ori__sgRNA_F (DNA)pX330A_screen_R,pA,SbfI,G2P_SbfI_F,MSCVmu promoter,MCS,BGH-rev (DNA),+25EF1 promoter,seq_CMV-PB R (DNA),NotI,AsiSI,PvuI,MfeI,HindIII,Bsu36I[oTP] seq PB-CRE Fwd (DNA),seq_PB-CRE Fwd (DNA),GP_screen F1 (DNA),+3GFP_P3_Rev (DNA),TurboGFP_int R (DNA),BspQI,SapIcopGFP,GP_screen R1 (DNA),FseIPaqCIGFP_P4_Int Fwd (DNA),PspOMI,ApaIT2A,NruIPflFI,Tth111I,PasI,BsiWI,RsrII,BstEIIPuroStuISexAIWHV-5R (DNA)HincIIEGFP-C-Re (DNA),pCI-rev (DNA),seq_plas_SV40_R (DNA),+4BarIPasIEcoRVAjuIEco53kISacICspCIAjuI,SphIKflI,BarI,BsaBIqPCR_F,BsmI

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    10899