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PEA1-Nuc Puro HEK3 CTT ins
  • Sequence Map
    Editing disabled on read-only sequence.
    aacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgattgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacactcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactPciIAflIIIU6 promoterhU6-F20406080100120140160
    cgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccGGCCCAGACTGAGCACGTGAgttttagagctagaaatagcgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaacacctttcctgctttgtggCCGGGTCTGACTCGTGCACTcaaaatctcgatctttatcgPmlIU6 promoterLKO.1 5'HEK3 gRNAgRNA scaffold180200220240260280300320340
    aagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcTCTGCCATCAAAGCGTGCTCAGTCTGtttttttgttctagtccgtttttagcgcgtgcgccaattctgcagacaaatggctctagaggtacccgttacataacttacggtaaatggcccttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgAGACGGTAGTTTCGCACGAGTCAGACaaaaaaacaagatcaggcaaaaatcgcgcacgcggttaagacgtctgtttaccgagatctccatgggcaatgtattgaatgccatttaccgggKpnIXbaIAcc65IgRNA scaffoldCMV enhancerPE template360380400420440460480500520
    gcctggctgaccgcccaacgacccccgcccattgacgtcaatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggccccggaccgactggcgggttgctgggggcgggtaactgcagttatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggCMV enhancer540560580600620640660680700
    gcctggcattgtgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttcggaccgtaacacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaaSnaBIChicken B-actin promoterCMV enhancer720740760780800820840860
    gtgcagcgatgggggcggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcacgtcgctacccccgccccccccccccgcgcgcggtccgccccgccccgccccgctccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcChicken B-actin promoter8809009209409609801,0001,0201,040
    cggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggtgccgcccgccctcagcgacgcgcgacggaagcggggcacggggcgaggcggcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgactcgttctccattcccaaattccctaccahybrid intron1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcactttttttcaggttggaccggtgccaccatggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaaggtcaccaaccaccccataattacaaattaatggacctcgtggacggactttagtgaaaaaaagtccaacctggccacggtggtacctgatattcctggtgctgcctctgatgttcctagtactataactaatgtttctgctactgctattctaccggggtttcttcttcgccttccagAgeIPaqCIMD2YK4DH6DG8DY10KD12HD14ID16YK18DD20DD22KM24AP26KK28KR30KV321305-84143xFLAGhybrid intronSV40 NLSve...)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgcccataggtgcctcagggtcgtcggctgttcttcatgtcgtagccggacctgtagccgtggttgagacacccgacccggcactagtggctgctcatgttccacgggtcgttctttaagttccacgacccgttgtggctggccgtgtcgtagttcttcttggactagcctcgggacgGI34HG36VP38AA40DK42KY44SI46GL48DI50GT52NS54VG56WA58VI60TD62EY64KV66PS68KK70FK72VL74GN76TD78RH80SI82KK84NL86IG88AL901305-8414Cas91,4201,4401,4601,4801,5001,5201,5401,560
    tgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggaacaagctgtcgccgctttgtcggctccggtgggccgacttctcttggcggtcttcttctatgtggtctgccttcttggcctagacgatagacgttctctagaagtcgttgctctaccggttccacctgctgtcgaagaaggtgtctgaccttctcaggaaggaccaccttctcctBglIIPflMILF92DS94GE96TA98EA100TR102LK104RT106AR108RR110YT112RR114KN116RI118CY120LQ122EI124FS126NE128MA130KV132DD134SF136FH138RL140EE142SF144LV146EE148D1305-8414Cas91,5801,6001,6201,6401,6601,6801,7001,7201,740
    taagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcattcttcgtgctcgccgtggggtagaagccgttgtagcacctgctccaccggatggtgctcttcatggggtggtagatggtggactctttctttgaccacctgtcgtggctgttccggctggacgccgactagatagaccgggaccgggtgtactagttcaaggccccggtgaagK150KH152ER154HP156IF158GN160IV162DE164VA166YH168EK170YP172TI174YH176LR178KK180LV182DS184TD186KA188DL190RL192IY194LA196LA198HM200IK202FR204GH206F1305-8414Cas91,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcggactagctcccgctggacttggggctgttgtcgctgcacctgttcgacaagtaggtcgaccacgtctggatgttggtcgacaagctccttttggggtagttgcggtcgccgcacctgcggttccggtaggacagacggtctgactcgttctcgtctgccgaccttttagactagcBmgBIL208IE210GD212LN214PD216NS218DV220DK222LF224IQ226LV228QT230YN232QL234FE236EN238PI240NA242SG244VD246AK248AI250LS252AR254LS256KS258RR260LE262NL264I1305-8414Cas91,9401,9601,9802,0002,0202,0402,0602,0802,100
    cccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagggtcgacgggccgctcttcttcttaccggacaagcctttggactaacgggactcggacccggactgggggttgaagttctcgttgaagctggaccggctcctacggtttgacgtcgactcgttcctgtggatgctgctgctggacctgttggacgaccgggtctagccgctggtA266QL268PG270EK272KN274GL276FG278NL280IA282LS284LG286LT288PN290FK292SN294FD296LA298ED300AK302LQ304LS306KD308TY310DD312DL314DN316LL318AQ320IG322DQ3241305-8414Cas92,1202,1402,1602,1802,2002,2202,2402,260
    gtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcatgcggctggacaaagaccggcggttcttggacaggctgcggtaggacgactcgctgtaggactctcacttgtggctctagtggttccggggggactcgcggagatactagttctctatgctgctcgtggtggtcctggactgggacgactttcgagagcacgccgtcgtcgacYA326DL328FL330AA332KN334LS336DA338IL340LS342DI344LR346VN348TE350IT352KA354PL356SA358SM360IK362RY364DE366HH368QD370LT372LL374KA376LV378RQ380QL3821305-8414Cas92,2802,3002,3202,3402,3602,3802,4002,4202,440
    cctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcggactcttcatgtttctctaaaagaagctggtctcgttcttgccgatgcggccgatgtaactgccgcctcggtcggtccttctcaagatgttcaagtagttcgggtaggaccttttctacctgccgtggctccttgacgagcacttcgacttgtctctcctggacgacgccttcgPE384KY386KE388IF390FD392QS394KN396GY398AG400YI402DG404GA406SQ408EE410FY412KF414IK416PI418LE420KM422DG424TE426EL428LV430KL432NR434ED436LL438RK4401305-8414Cas92,4602,4802,5002,5202,5402,5602,5802,6002,620
    agcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagtcgcctggaagctgttgccgtcgtagggggtggtctaggtggaccctctcgacgtgcggtaagacgccgccgtccttctaaaaatgggtaaggacttcctgttggcccttttctagctcttctaggactggaaggcgtaggggatgatgcacccgggagaccggtcccctttgtcQR442TF444DN446GS448IP450HQ452IH454LG456EL458HA460IL462RR464QE466DF468YP470FL472KD474NR476EK478IE480KI482LT484FR486IP488YY490VG492PL494AR496GN498S1305-8414Cas92,6402,6602,6802,7002,7202,7402,7602,7802,800
    cagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcgtctaagcggacctactggtctttctcgctcctttggtagtgggggaccttgaagctccttcaccacctgttcccgcgaaggcgggtctcgaagtagctcgcctactggttgaagctattcttggacgggttgctcttccacgacgggttcgtgtcggacgacatgctcatgaagR500FA502WM504TR506KS508EE510TI512TP514WN516FE518EV520VD522KG524AS526AQ528SF530IE532RM534TN536FD538KN540LP542NE544KV546LP548KH550SL552LY554EY556F1305-8414Cas92,8202,8402,8602,8802,9002,9202,9402,960
    accgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgacttggcacatattgctcgactggtttcactttatgcactggctcccttactctttcgggcggaaggactcgccgctcgtctttttccggtagcacctggacgacaagttctggttggcctttcactggcacttcgtcgactttctcctgatgaagttcttttagctcacgaagctgaT558VY560NE562LT564KV566KY568VT570EG572MR574KP576AF578LS580GE582QK584KA586IV588DL590LF592KT594NR596KV598TV600KQ602LK604ED606YF608KK610IE612CF614D1305-8414Cas92,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacgggcacctttagaggccgcaccttctagccaagttgcggagggacccgtgtatggtgctagacgacttttaatagttcctgttcctgaaggacctgttactccttttgctcctgtaagaccttctatagcacgactgggactgtgacaaactcctgtctctctactagctccttgcEcoRVS616VE618IS620GV622ED624RF626NA628SL630GT632YH634DL636LK638II640KD642KD644FL646DN648EE650NE652DI654LE656DI658VL660TL662TL664FE666DR668EM670IE672ER6741305-8414Cas93,1603,1803,2003,2203,2403,2603,2803,3003,320
    gctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttccgacttttggatacgggtggacaagctgctgtttcactacttcgtcgacttcgccgcctctatgtggccgaccccgtccgactcggccttcgactagttgccgtaggccctgttcgtcaggccgttctgttaggacctaaaggacttcaggctgccgaagcggttgtctttgaagLK676TY678AH680LF682DD684KV686MK688QL690KR692RR694YT696GW698GR700LS702RK704LI706NG708IR710DK712QS714GK716TI718LD720FL722KS724DG726FA728NR730NF7321305-8414Cas93,3403,3603,3803,4003,4203,4403,4603,4803,500
    atgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatggtacgtcgactaggtgctgctgtcggactggaaatttctcctgtaggtctttcgggtccacaggccggtcccgctatcggacgtgctcgtgtaacggttagaccggccgtcggggcggtaattcttcccgtaggacgtctgtcacttccaccacctgctcgagcactttcactaccAjuISacIEco53kIMQ734LI736HD738DS740LT742FK744ED746IQ748KA750QV752SG754QG756DS758LH760EH762IA764NL766AG768SP770AI772KK774GI776LQ778TV780KV782VD784EL786VK788VM7901305-8414Cas93,5203,5403,5603,5803,6003,6203,6403,660
    gccggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaacggccgtgttcgggctcttgtagcactagctttaccggtctctcttggtctggtgggtcttccctgtcttcttgtcggcgctctcttacttcgcctagcttctcccgtagtttctcgacccgtcggtctaggactttcttgtggggcaccttttgtgggtcgacgtcttgctcttGR792HK794PE796NI798VI800EM802AR804EN806QT808TQ810KG812QK814NS816RE818RM820KR822IE824EG826IK828EL830GS832QI834LK836EH838PV840EN842TQ844LQ846NE848K1305-8414Cas93,6803,7003,7203,7403,7603,7803,8003,8203,840
    gctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgaccgacatggacatgatggacgtcttacccgccctatacatgcacctggtccttgacctgtagttggccgacaggctgatgctacacctggtatagcacggagtctcgaaagacttcctgctgaggtagctgttgttccacgactggtcttcgctgttcttggccccgttctcgctgPflMIL850YL852YY854LQ856NG858RD860MY862VD864QE866LD868IN870RL872SD874YD876VD878HI880VP882QS884FL886KD888DS890ID892NK894VL896TR898SD900KN902RG904KS906D1305-8414Cas93,8603,8803,9003,9203,9403,9603,9804,0004,020
    aacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagattgcacgggaggcttctccagcacttcttctacttcttgatgaccgccgtcgacgacttgcggttcgactaatgggtctctttcaagctgttagactggttccggctctctccgccggactcgcttgacctattccggccgaagtagttctctgtcgaccacctttgggccgtctCspCIN908VP910SE912EV914VK916KM918KN920YW922RQ924LL926NA928KL930IT932QR934KF936DN938LT940KA942ER944GG946LS948EL950DK952AG954FI956KR958QL960VE962TR964Q1305-8414Cas94,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccaagtgtttcgtgcaccgtgtctaggacctgagggcctacttgtgattcatgctgctcttactgttcgactaggcccttcactttcactagtgggacttcaggttcgaccacaggctaaaggccttcctaaaggtcaaaatgtttcacgcgctctagttgttgatggtggtgcgggtPmlII966TK968HV970AQ972IL974DS976RM978NT980KY982DE984ND986KL988IR990EV992KV994IT996LK998SK1000LV1002SD1004FR1006KD1008FQ1010FY1012KV1014RE1016IN1018NY1020HH1022AH10241305-8414Cas94,2204,2404,2604,2804,3004,3204,3404,360
    cgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatggctgcggatggacttgcggcagcacccttggcgggactagtttttcatgggattcgacctttcgctcaagcacatgccgctgatgttccacatgctgcacgccttctactagcggttctcgctcgtcctttagccgttccgatggcggttcatgaagaagatgtcgttgtagtacAjuIBmgBIDA1026YL1028NA1030VV1032GT1034AL1036IK1038KY1040PK1042LE1044SE1046FV1048YG1050DY1052KV1054YD1056VR1058KM1060IA1062KS1064EQ1066EI1068GK1070AT1072AK1074YF1076FY1078SN1080IM10821305-8414Cas94,3804,4004,4204,4404,4604,4804,5004,5204,540
    aactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagattgaaaaagttctggctctaatgggaccggttgccgctctaggccttcgccggagactagctctgtttgccgctttggcccctctagcacaccctattcccggccctaaaacggtggcacgcctttcacgactcgtacggggttcacttatagcactttttctggctccacgtctSphINF1084FK1086TE1088IT1090LA1092NG1094EI1096RK1098RP1100LI1102ET1104NG1106ET1108GE1110IV1112WD1114KG1116RD1118FA1120TV1122RK1124VL1126SM1128PQ1130VN1132IV1134KK1136TE1138VQ11401305-8414Cas94,5604,5804,6004,6204,6404,6604,6804,7004,720
    caggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgtccgccgaagtcgtttctcagataggacgggttctccttgtcgctattcgactagcggtctttcttcctgaccctgggattcttcatgccgccgaagctgtcggggtggcaccggataagacacgaccaccaccggtttcaccttttcccgttcaggttctttgacttctcacaKflITG1142GF1144SK1146ES1148IL1150PK1152RN1154SD1156KL1158IA1160RK1162KD1164WD1166PK1168KY1170GG1172FD1174SP1176TV1178AY1180SV1182LV1184VA1186KV1188EK1190GK1192SK1194KL1196KS1198V1305-8414Cas94,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcctttctcgacgacccctagtggtagtacctttcttcgtcgaagctcttcttagggtagctgaaagaccttcggttcccgatgtttcttcactttttcctggactagtagttcgacggattcatgagggacaagctcgaccttttgccggccttctcttacgaccggagacggccgBsmIXcmIK1200EL1202LG1204IT1206IM1208ER1210SS1212FE1214KN1216PI1218DF1220LE1222AK1224GY1226KE1228VK1230KD1232LI1234IK1236LP1238KY1240SL1242FE1244LE1246NG1248RK1250RM1252LA1254SA1256G1305-8414Cas94,9204,9404,9604,9805,0005,0205,0405,060
    gaactgcagaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctcttgacgtcttccctttgcttgaccgggacgggaggtttatacacttgaaggacatggaccggtcggtgatactcttcgacttcccgagggggctcctattactcgtctttgtcgacaaacaccttgtcgtgttcgtgatggacctgctctagtagctcgtctagtcgctcaagaE1258LQ1260KG1262NE1264LA1266LP1268SK1270YV1272NF1274LY1276LA1278SH1280YE1282KL1284KG1286SP1288ED1290NE1292QK1294QL1296FV1298EQ1300HK1302HY1304LD1306EI1308IE1310QI1312SE1314F1305-8414Cas95,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagagggttctctcactaggaccggctgcgattagacctgtttcacgacaggcggatgttgttcgtggccctattcgggtagtctctcgtccggctcttatagtaggtggacaaatgggactggttagaccctcggggacggcggaagttcatgaaactgtggtggtagctggccttctcS1316KR1318VI1320LA1322DA1324NL1326DK1328VL1330SA1332YN1334KH1336RD1338KP1340IR1342EQ1344AE1346NI1348IH1350LF1352TL1354TN1356LG1358AP1360AA1362FK1364YF1366DT1368TI1370DR1372KR13741305-8414Cas95,2605,2805,3005,3205,3405,3605,3805,4005,420
    gtacaccagcacgaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggtgactctggaggatctagcggaggatcctctggcagcgagacaccaggaacaagcgagtcagcaacaccagagagcagtcatgtggtcgtgctttctccacgacctgcggtgggactaggtggtctcgtagtggccggacatgctctgtgcctagctggacagagtcgaccctccactgagacctcctagatcgcctcctaggagaccgtcgctctgtggtccttgttcgctcagtcgttgtggtctctcgtcaYT1376ST1378KE1380VL1382DA1384TL1386IH1388QS1390IT1392GL1394YE1396TR1398ID1400LS1402QL1404GG1406DS1408GG1410SS1412GG1414SS1416GS1418ET1420PG1422TS1424ES1426AT1428PE1430SS14321305-8414Cas9reverse transcriptase5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggcggcagcagcggcggcagcagcaccctaaatatagaagatgagtatcggctacatgagacctcaaaagagccagatgtttctctagggtccacatggctgtctgattttcctcaggcctgggcggaaaccgggggcatgggactggcagttcgccaagctcctctgatcatacccgccgtcgtcgccgccgtcgtcgtgggatttatatcttctactcatagccgatgtactctggagttttctcggtctacaaagagatcccaggtgtaccgacagactaaaaggagtccggacccgcctttggcccccgtaccctgaccgtcaagcggttcgaggagactagtatgBsu36IGG1434SS1436GG1438SS1440TL1442NI1444ED1446EY1448RL1450HE1452TS1454KE1456PD1458VS1460LG1462ST1464WL1466SD1468FP1470QA1472WA1474ET1476GG1478MG1480LA1482VR1484QA1486PL1488II14901305-8414reverse transcriptase5,6205,6405,6605,6805,7005,7205,7405,760
    ctctgaaagcaacctctacccccgtgtccataaaacaataccccatgtcacaagaagccagactggggatcaagccccacatacagagactgttggaccagggaatactggtaccctgccagtccccctggaacacgcccctgctacccgttaagaaaccagggactaatgattagagactttcgttggagatgggggcacaggtattttgttatggggtacagtgttcttcggtctgacccctagttcggggtgtatgtctctgacaacctggtcccttatgaccatgggacggtcagggggaccttgtgcggggacgatgggcaattctttggtccctgattactaatKpnIAcc65IPL1492KA1494TS1496TP1498VS1500IK1502QY1504PM1506SQ1508EA1510RL1512GI1514KP1516HI1518QR1520LL1522DQ1524GI1526LV1528PC1530QS1532PW1534NT1536PL1538LP1540VK1542KP1544GT1546ND1548Y1305-8414reverse transcriptase5,7805,8005,8205,8405,8605,8805,9005,9205,940
    taggcctgtccaggatctgagagaagtcaacaagcgggtggaggacatccaccccaccgtgcccaacccttacaacctcttgagcgggctcccaccgtcccaccagtggtacactgtgcttgatttaaaggatgcctttttctgcctgagactccaccccaccagtcagcctctcatccggacaggtcctagactctcttcagttgttcgcccacctcctgtaggtggggtggcacgggttgggaatgttggagaactcgcccgagggtggcagggtggtcaccatgtgacacgaactaaatttcctacggaaaaagacggactctgaggtggggtggtcagtcggagagHincIIR1550PV1552QD1554LR1556EV1558NK1560RV1562ED1564IH1566PT1568VP1570NP1572YN1574LL1576SG1578LP1580PS1582HQ1584WY1586TV1588LD1590LK1592DA1594FF1596CL1598RL1600HP1602TS1604QP1606L1305-8414reverse transcriptase5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttcgcctttgagtggagagatccagagatgggaatctcaggacaattgacctggaccagactcccacagggtttcaaaaacagtcccaccctgtttaatgaggcactgcacagagacctagcagacttccggatccagcacccagacttgatcctgctacagtacgtggatgactaagcggaaactcacctctctaggtctctacccttagagtcctgttaactggacctggtctgagggtgtcccaaagtttttgtcagggtgggacaaattactccgtgacgtgtctctggatcgtctgaaggcctaggtcgtgggtctgaactaggacgatgtcatgcacctactgaMfeIF1608AF1610EW1612RD1614PE1616MG1618IS1620GQ1622LT1624WT1626RL1628PQ1630GF1632KN1634SP1636TL1638FN1640EA1642LH1644RD1646LA1648DF1650RI1652QH1654PD1656LI1658LL1660QY1662VD1664D1305-8414reverse transcriptase6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tactgctggccgccacttctgagctagactgccaacaaggtactcgggccctgttacaaaccctagggaacctcgggtatcgggcctcggccaagaaagcccaaatttgccagaaacaggtcaagtatctggggtatcttctaaaagagggtcagagatggctgactgaggccagatgacgaccggcggtgaagactcgatctgacggttgttccatgagcccgggacaatgtttgggatcccttggagcccatagcccggagccggttctttcgggtttaaacggtctttgtccagttcatagaccccatagaagattttctcccagtctctaccgactgactccggtcAvrIIL1666LL1668AA1670TS1672EL1674DC1676QQ1678GT1680RA1682LL1684QT1686LG1688NL1690GY1692RA1694SA1696KK1698AQ1700IC1702QK1704QV1706KY1708LG1710YL1712LK1714EG1716QR1718WL1720TE1722AR17241305-8414reverse transcriptase6,3206,3406,3606,3806,4006,4206,4406,460
    aaaagagactgtgatggggcagcctactccaaagacccctcgacaactaagggagttcctagggaaggcaggcttctgtcgcctcttcatccctgggtttgcagaaatggcagcccccctgtaccctctcaccaaaccggggactctgtttaattggggcccagaccaacaaaagttttctctgacactaccccgtcggatgaggtttctggggagctgttgattccctcaaggatcccttccgtccgaagacagcggagaagtagggacccaaacgtctttaccgtcggggggacatgggagagtggtttggcccctgagacaaattaaccccgggtctggttgttttcAvrIIKE1726TV1728MG1730QP1732TP1734KT1736PR1738QL1740RE1742FL1744GK1746AG1748FC1750RL1752FI1754PG1756FA1758EM1760AA1762PL1764YP1766LT1768KP1770GT1772LF1774NW1776GP1778DQ1780QK17821305-8414reverse transcriptase6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcctatcaagaaatcaagcaagcccttctaactgccccagccctggggttgccagatttgactaagccctttgaactctttgtcgacgagaagcagggctacgccaaaggtgtcctaacgcaaaaactgggaccttggcgtcggccggtggcctacctgtccaaaaagctagacccggatagttctttagttcgttcgggaagattgacggggtcgggaccccaacggtctaaactgattcgggaaacttgagaaacagctgctcttcgtcccgatgcggtttccacaggattgcgtttttgaccctggaaccgcagccggccaccggatggacaggtttttcgatctggAccISalIHincIIAY1784QE1786IK1788QA1790LL1792TA1794PA1796LG1798LP1800DL1802TK1804PF1806EL1808FV1810DE1812KQ1814GY1816AK1818GV1820LT1822QK1824LG1826PW1828RR1830PV1832AY1834LS1836KK1838LD18401305-8414reverse transcriptase6,6606,6806,7006,7206,7406,7606,7806,8006,820
    cagtagcagctgggtggcccccttgcctacggatggtagcagccattgccgtactgacaaaggatgcaggcaagctaaccatgggacagccactagtcattctggccccccatgcagtagaggcactagtcaaacaaccccccgaccgctggctttccaacgcccggatgactcagtcatcgtcgacccaccgggggaacggatgcctaccatcgtcggtaacggcatgactgtttcctacgtccgttcgattggtaccctgtcggtgatcagtaagaccggggggtacgtcatctccgtgatcagtttgttggggggctggcgaccgaaaggttgcgggcctactgagtPV1842AA1844GW1846PP1848CL1850RM1852VA1854AI1856AV1858LT1860KD1862AG1864KL1866TM1868GQ1870PL1872VI1874LA1876PH1878AV1880EA1882LV1884KQ1886PP1888DR1890WL1892SN1894AR1896MT1898H1305-8414reverse transcriptase6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctatcaggccttgcttttggacacggaccgggtccagttcggaccggtggtagccctgaacccggctacgctgctcccactgcctgaggaagggctgcaacacaactgccttgatatcctggccgaagcccacggaacccgacccgacctaacggaccagccgctcccagacgccgatagtccggaacgaaaacctgtgcctggcccaggtcaagcctggccaccatcgggacttgggccgatgcgacgagggtgacggactccttcccgacgttgtgttgacggaactataggaccggcttcgggtgccttgggctgggctggattgcctggtcggcgagggtctgcggAgeIEcoRVPflFIBsu36ITth111IY1900QA1902LL1904LD1906TD1908RV1910QF1912GP1914VV1916AL1918NP1920AT1922LL1924PL1926PE1928EG1930LQ1932HN1934CL1936DI1938LA1940EA1942HG1944TR1946PD1948LT1950DQ1952PL1954PD1956A1305-8414reverse transcriptase7,0207,0407,0607,0807,1007,1207,1407,160
    gaccacacctggtacacggatggaagcagtctcttacaagagggacagcgtaaggcgggagctgcggtgaccaccgagaccgaggtaatctgggctaaagccctgccagccgggacatccgctcagcgggctgaactgatagcactcacccaggccctaaagatggcagaaggtactggtgtggaccatgtgcctaccttcgtcagagaatgttctccctgtcgcattccgccctcgacgccactggtggctctggctccattagacccgatttcgggacggtcggccctgtaggcgagtcgcccgacttgactatcgtgagtgggtccgggatttctaccgtcttccatSexAIBstEIID1958HT1960WY1962TD1964GS1966SL1968LQ1970EG1972QR1974KA1976GA1978AV1980TT1982ET1984EV1986IW1988AK1990AL1992PA1994GT1996SA1998QR2000AE2002LI2004AL2006TQ2008AL2010KM2012AE2014G1305-8414reverse transcriptase7,1807,2007,2207,2407,2607,2807,3007,3207,340
    agaagctaaatgtttatactgatagccgttatgcttttgctactgcccatatccatggagaaatatacagaaggcgtgggtggctcacatcagaaggcaaagagatcaaaaataaagacgagatcttggccctactaaaagccctctttctgcccaaaagacttagcataatccatcttcgatttacaaatatgactatcggcaatacgaaaacgatgacgggtataggtacctctttatatgtcttccgcacccaccgagtgtagtcttccgtttctctagtttttatttctgctctagaaccgggatgattttcgggagaaagacgggttttctgaatcgtattaggtBglIIK2016KL2018NV2020YT2022DS2024RY2026AF2028AT2030AH2032IH2034GE2036IY2038RR2040RG2042WL2044TS2046EG2048KE2050IK2052NK2054DE2056IL2058AL2060LK2062AL2064FL2066PK2068RL2070SI2072IH20741305-8414reverse transcriptase7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttgtccaggacatcaaaagggacacagcgccgaggctagaggcaaccggatggctgaccaagcggcccgaaaggcagccatcacagagactccagacacctctaccctcctcatagaaaattcatcaccctctggcggctcaaaaagaaccgccgacggcagcgaattcgagaaaaacaggtcctgtagttttccctgtgtcgcggctccgatctccgttggcctaccgactggttcgccgggctttccgtcggtagtgtctctgaggtctgtggagatgggaggagtatcttttaagtagtgggagaccgccgagtttttcttggcggctgccgtcgcttaagctctttCP2076GH2078QK2080GH2082SA2084EA2086RG2088NR2090MA2092DQ2094AA2096RK2098AA2100IT2102ET2104PD2106TS2108TL2110LI2112EN2114SS2116PS2118GG2120SK2122RT2124AD2126GS2128EF2130EK21321305-8414reverse transcriptase7,5407,5607,5807,6007,6207,6407,6607,6807,700
    aggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaaggaattcggcagtggagagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtactccggccgccggtgctttttccggccggtccgttttttctttttccttaagccgtcacctctcccgtctccttcagacgattgtacgccactgcagctcctcttaggaccgggttactggctcatgttcgggtgccacgcggagcggtgggcgctgctgcaggggtcccggcatgFseIBsiWIPflFITth111IRP2134AA2136TK2138KA2140GQ2142AK2144KK2146KE2148FG2150SG2152EG2154RG2156SL2158LT2160CG2162DV2164EE2166NP2168GP2170MT2172EY2174KP2176TV2178RL2180AT2182RD2184DV2186PR2188AV21901305-8414PuroRT2Anucleoplasmin NLSPuro-R7,7207,7407,7607,7807,8007,8207,8407,860
    gcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgcccgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccgttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggSacIIBstEIIRT2192LA2194AA2196FA2198DY2200PA2202TR2204HT2206VD2208PD2210RH2212IE2214RV2216TE2218LQ2220EL2222FL2224TR2226VG2228LD2230IG2232KV2234WV2236AD2238DG2240AA2242VA2244VW2246TT2248P1305-8414PuroR7,8807,9007,9207,9407,9607,9808,0008,0208,040
    ggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggagtctcgcccgaccaccagggcaagcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcgggcgcaccaaggaccggtggcagcctcagagcgggctggtggtcccgttcE2250SV2252EA2254GA2256VF2258AE2260IG2262PR2264MA2266EL2268SG2270SR2272LA2274AQ2276QQ2278ME2280GL2282LA2284PH2286RP2288KE2290PA2292WF2294LA2296TV2298GV2300SP2302DH2304QG2306K1305-8414PuroR8,0608,0808,1008,1208,1408,1608,1808,2008,220
    ggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtggcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgtSexAIG2308LG2310SA2312VV2314LP2316GV2318EA2320AE2322RA2324GV2326PA2328FL2330ET2332SA2334PR2336NL2338PF2340YE2342RL2344GF2346TV2348TA2350DV2352EV2354PE2356GP2358RT2360WC2362MT2364R1305-8414PuroRPuro-F8,2408,2608,2808,3008,3208,3408,3608,3808,400
    agcccggtgcctgagaattctaactagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtcgggccacggactcttaagattgatctcgagcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccSacIEco53kIK2366PG2368A*23701305-8414bGH poly(A) signalBGH-revPuroR8,4208,4408,4608,4808,5008,5208,5408,560
    tgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggagcggccgctctagaactagtggatcccccgggctgcaggaattcgatacgaatcctgcaggaaaaaagcaccgactcggtgccactttttcaagtacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctcttatcgtccgtacgacccctcgccggcgagatcttgatcacctagggggcccgacgtccttaagctatgcttaggacgtccttttttcgtggctgagccacggtgaaaaagttcaNotISbfISphIXbaIbGH poly(A) signalgRNA scaffoldSK primerpBlue...ptSK8,5808,6008,6208,6408,6608,6808,7008,7208,740
    tgataacggactagccttattttaacttgctatttctagctctaaaacAATCTTGCAGTGTTGCCTGACggtgtttcgtcctttccacaagatatataaagccaagaaatcgaaatactttcaagttacggtaagcatatgatagtccattttaaaacataattttaaaactgcaactattgcctgatcggaataaaattgaacgataaagatcgagattttgTTAGAACGTCACAACGGACTGccacaaagcaggaaaggtgttctatatatttcggttctttagctttatgaaagttcaatgccattcgtatactatcaggtaaaattttgtattaaaattttgacgtU6 promotergRNA scaffoldSham gRNA8,7608,7808,8008,8208,8408,8608,8808,9008,920
    aactacccaagaaattattactttctacgtcacgtattttgtactaatatctttgtgtttacagtcaaattaattccaattatctctctaacagccttgtatcgtatatgcaaatatgaaggaatcatgggaaataggccctcgcggccgcaggaacccctagtgatggagttggttgatgggttctttaataatgaaagatgcagtgcataaaacatgattatagaaacacaaatgtcagtttaattaaggttaatagagagattgtcggaacatagcatatacgtttatacttccttagtaccctttatccgggagcgccggcgtccttggggatcactacctcaaccNotISfiIU6 promoterAAV2 ITRAAV2 ITR8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcSbfISrfISrfIAAV2 ITRAAV2 ITR9,1209,1409,1609,1809,2009,2209,2409,260
    tcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcggaatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgf1 oriF1ori-R9,2809,3009,3209,3409,3609,3809,4009,4209,440
    tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactcagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgagf1 oriF1ori-F9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcccatagagcccgataagaaaactaaatattccctaaaacggctaaagccagataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcgggPsiISspIf1 oripRS-marker9,6409,6609,6809,7009,7209,7409,7609,7809,800
    cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacBsmBIBsmBIEsp3IEsp3IpGEX 3'pBRforEco9,8209,8409,8609,8809,9009,9209,9409,960
    tcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaSspIAmpR promoterAmpR9,98010,00010,02010,04010,06010,08010,10010,12010,140
    ccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaAmpR10,16010,18010,20010,22010,24010,26010,28010,30010,320
    ccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacAmpRAmp-R10,34010,36010,38010,40010,42010,44010,46010,48010,500
    ctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgccgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggPvuIAmpR10,52010,54010,56010,58010,60010,62010,64010,660
    tgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacctFspIAmpR10,68010,70010,72010,74010,76010,78010,80010,82010,840
    agccgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatacttttcggcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaaSacIIAmpR10,86010,88010,90010,92010,94010,96010,98011,00011,020
    agattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgctttctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaaori11,04011,06011,08011,10011,12011,14011,16011,18011,200
    gcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctccgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagori11,22011,24011,26011,28011,30011,32011,34011,360
    gctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagccgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgori11,38011,40011,42011,44011,46011,48011,50011,52011,540
    gtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacpBR322ori-Fori11,56011,58011,60011,62011,64011,66011,68011,70011,720
    ctcgtcaggggggcggagcctatggaaagagcagtccccccgcctcggatacctttori11,73011,74011,750
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    11753 bp850090009500100001050011000115005001000150020002500Cas93xFLAG1305-8414hU6-F,U6 promoter,LKO.1 5',HEK3 gRNA,gRNA scaffold,PE template,CMV enhancer,+6Chicken B-actin promoter,SnaBIhybrid intron,+4BglIIPflMIBmgBIU6 promoterAAV2 ITR,AAV2 ITR,SfiI,NotI,SrfI,SrfIF1ori-R,SbfIf1 ori,F1ori-FpRS-marker,PsiI,SspIpGEX 3',pBRforEco,BsmBI,Esp3I,BsmBI,Esp3IAmpR promoter,SspIAmpR,Amp-R,PvuIFspI,SacIIori,pBR322ori-F

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    11753