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Sinker Node Archetype v5
  • Sequence Map
    Editing disabled on read-only sequence.
    taccgagctcgaattcactgCACCTGCCAGTCAGTTCTACACTCTTTCCCTACACGACGCTCTTCCGATCTTGACAACCTCGTTTGTCGAATGTAAAATACAATGCDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDNNNNNNNATTTTANNNNNNNATCTACAACatggctcgagcttaagtgacGTGGACGGTCAGTCAAGATGTGAGAAAGGGATGTGCTGCGAGAAGGCTAGAACTGTTGGAGCAAACAGCTTACATTTTATGTTACGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHNNNNNNNTAAAATNNNNNNNTAGATGTTGAluIApoIBsgIBsrIBtsIDpnIEarIHgaIMboISacISapIBanIIBfuAIBfuCIBsmFIBspMIBspQIBtsαIDpnIIEcoRIMboIIMluCIPaqCITscAITspRIHpy99ISau3AIBsiHKAIEco53kIHpy188IHpyCH4VTsp509IBsp1286IBtsIMutISinkerStrong Promoter5prime primerAnnealpUC19 OverlapRC CRIS...inding20406080100120140160
    cgtcccgcaaggatgcgggtctgtttacccctatttcaaccggccgcctcgcggccggtttttttttttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaGACGAACAATAAGGCCTCCCTAACGGGGGGCCTTTTTTATTGATAACAAAAGCAGTCgcagggcgttcctacgcccagacaaatggggataaagttggccggcggagcgccggccaaaaaaaaaaacaagtcttgcgagccaacggcggcccgcaaaaaataaccactcttaggtCTGCTTGTTATTCCGGAGGGATTGCCCCCCGGAAAAAATAACTATTGTTTTCGTCAGBsaIEagIEagIFauIFauIFokIHphINciIStuITfiIBcoDIBsiEIBsiEIBsmAIBssKIBstUIBtsCIHinfINlaIVScrFISfaNISau96IStyD4IHpy188IEcoO109IHpy166IIHpyCH4IIImicF M7.4T0 Terminator (from O'Connor et al.)L3S1P13180200220240260280300320340
    GAGACCGGTCTCGTGACCAGTGCAGGTGgccgtcgttttacaacgtcCTCTGGCCAGAGCACTGGTCACGTCCACcggcagcaaaatgttgcagAgeIBsaIBsgIBsrIBceAIBcoDIBfuAIBsaWIBsmAIBspMIBssSIPaqCITscAITspRIBssSαIHpy99ITsp45IHpyCH4VBtsIMutIHpyCH4IVpUC19 Overlap360380
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    • Description
    Editing disabled on read-only sequence.
    Sinker Node Archetype v5 (397 bp)AgeIAluIApoIBsaIBsaIBsgIBsgIBsrIBsrIBtsIDpnIEagIEagIEarIFauIFauIFokIHgaIHphIMboINciISacISapIStuITfiIBanIIBceAIBcoDIBcoDIBfuAIBfuAIBfuCIBsaWIBsiEIBsiEIBsmAIBsmAIBsmFIBspMIBspMIBspQIBssKIBssSIBstUIBtsCIBtsαIDpnIIEcoRIHinfIMboIIMluCINlaIVPaqCIPaqCIScrFISfaNITscAITscAITspRITspRIBssSαIHpy99IHpy99ISau3AISau96IStyD4ITsp45IBsiHKAIEco53kIHpy188IHpy188IHpyCH4VHpyCH4VTsp509IBsp1286IBtsIMutIBtsIMutIEcoO109IHpy166IIHpyCH4IVHpyCH4III50100150200250300350SinkermicF M7.4T0 Termi...et al.)L3S1P13Stron...oter5prim...imerAnnealS5Rev_v4 (DNA)S1Rev_v4 (DNA)S3Rev_v4 (DNA)S2Rev_v4 (DNA)S4Rev_v4 (DNA)S4For_v4 (DNA)S2For_v4 (DNA)S3For_v4 (DNA)S1For_v4 (DNA)S5For_v4 (DNA)Sinker Node Archetype v5 (397 bp)

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    397