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pZCS23 (eft-3 CRE in pUC19)
  • Sequence Map
    Editing disabled on read-only sequence.
    gtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgacatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactAhdIBmrIBsrDIAmpR20406080100120140160
    tttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtori180200220240260280300320340
    aaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgcttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgAcuIori360380400420440460480500520
    taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgaAlwNIori540560580600620640660680700
    atgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtoripBR322ori-F720740760780800820840860
    ggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcaccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtPciIAflIIIori8809009209409609801,0001,0201,040
    gcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacgctcagtcactcgctccttcgccttctcgcgggttatgcgtttggcggagaggggcgcgcaaccggctaagtaattacgtcgaccgtgctgtccaaagggctgacctttcgcccgtcactcgcgttgcgttaattacactcaatcgagtgagtaatccgtggggtccgaaatgtSapIBsaXIBspQICAP binding siteL44401,0601,0801,1001,1201,1401,1601,1801,2001,220
    ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcgactctagaggatcccGCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTgaaatacgaaggccgagcatacaacacaccttaacactcgcctattgttaaagtgtgtcctttgtcgatactggtactaatgcggttcgaacgtacggacgtccagctgagatctcctagggCGTGGAAACCAGAAAATAACAGTTGAAGGTAACCAAGAAGGTAACAAAGACAAAccIPstISalISbfISphIXbaIBamHIBfuAIBspMIeft-3 promoterlac promoterM13/pUC Reverselac operatorM13 ReverseM13 rev1,2401,2601,2801,3001,3201,3401,3601,3801,400
    AAATTAATGAATTTTTCATAAAATAAAGACATTATACAATATAAAAATGAAGAATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGTTTAATTACTTAAAAAGTATTTTATTTCTGTAATATGTTATATTTTTACTTCTTAAATAACTTTTATTTGACGGTCTCTCTTTTTCATACGTTGTGAGGGCGGCTCTCACAAACTTTACCACATGCCATGTAAAAGCACGATCCTCAATCTACACGTCCGTCGTTGCTCTCCCCCBsgINmeAIIIeft-3 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    AGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTTTTCTGGTCATCTGACTAATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCTCTAAAAAAACCCGGAACACTTTAATTGCACTCAAAAGACCAGTAGACTGATTAGTACAACCAAAAAACAACCAAATAAAACAAAAATAGAAACAAAAATAGGTCTAATCCTTTAAATTTAAAATACTTAAATATTACTCCAGTTTGTAAGTCAGGGTCGCAAAAAGGACAAGPsiIBsmFIeft-3 promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAACAAATGTTCTAACTGTCTTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGAGTGACAAATCAGCTTAAAAATAAAATCCGAAAGTTGTTTACAAGATTGACAGAATAAACACTGGAGTGAAAAATATAAAAAAATTAAAAATTTTTATAATCTTCAAAGATCCTATTAAAAAAGCTGAAAATAAGAGAGATGGCAGGCGTGAGAAGAATGAAAATTTAATTTAACeft-3 promoter1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TTTTTTTTTCAGTTGGGAAACACTTTGCTCTAGAaaaaATGTCCCGTCGTCGTAAGGCCAACCCAACCAAGCTCTCCGAGAACGCCAAGAAGAAGAAGCCAGAGACCGGAATGTCTAACCTCCTTACTGTACATCAAAACCTTCCAGCTCTTCCAGTCGATGCTACCTCCGATGAAAAAAAAAAGTCAACCCTTTGTGAAACGAGATCTttttTACAGGGCAGCAGCATTCCGGTTGGGTTGGTTCGAGAGGCTCTTGCGGTTCTTCTTCTTCGGTCTCTGGCCTTACAGATTGGAGGAATGACATGTAGTTTTGGAAGGTCGAGAAGGTCAGCTACGATGGAGGCTACTSapIXbaIBsmFIBspQIBsrGIM1S2N3L4L5T6V7H8Q9N10L11P12A13L14P15V16D17A18T19S20D21E222036-2293M1S2R3R4R5K6A7N8P9T10K11L12S13E14N15A16K17K18K19K20P21E22T23G241964-2035NLSCre recombinaseeft-3 promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GGTTCGTAAGAACCTTATGGATATGTTCCGTGATCGTCAAGCTTTCTCTGAGCACACTTGGAAGATGCTTCTTTCCGTTTGCCGTTCCTGGGCTGCTTGGTGCAAGCTTAATAACCGCAAGTGGTTCCCAGCTGAACCAGAGGATGTCCGTGATTACCTTCTTTACCTTCAAGCTCCAAGCATTCTTGGAATACCTATACAAGGCACTAGCAGTTCGAAAGAGACTCGTGTGAACCTTCTACGAAGAAAGGCAAACGGCAAGGACCCGACGAACCACGTTCGAATTATTGGCGTTCACCAAGGGTCGACTTGGTCTCCTACAGGCACTAATGGAAGAAATGGAAGTTCGAV23R24K25N26L27M28D29M30F31R32D33R34Q35A36F37S38E39H40T41W42K43M44L45L46S47V48C49R50S51W52A53A54W55C56K57L58N59N60R61K62W63F64P65A66E67P68E69D70V71R72D73Y74L75L76Y77L78Q79A802036-2293Cre recombinase2,1202,1402,1602,1802,2002,2202,2402,260
    CGTGGACTTGCTGTTAAGgtaagtttaaacatatatatactaactaaccctgattatttaaattttcagACTATCCAACAACATCTTGGACAACTTAACATGCTTCACCGTCGTTCTGGACTTCCACGTCCATCTGATTCCAACGCTGTTTCCCTTGTTATGCGCCGCATCCGTAGCACCTGAACGACAATTCcattcaaatttgtatatatatgattgattgggactaataaatttaaaagtcTGATAGGTTGTTGTAGAACCTGTTGAATTGTACGAAGTGGCAGCAAGACCTGAAGGTGCAGGTAGACTAAGGTTGCGACAAAGGGAACAATACGCGGCGTAGGCATXcmIBmgBIT1I2Q3Q4H5L6G7Q8L9N10M11L12H13R14R15S16G17L18P19R20P21S22D23S24N25A26V27S28L29V30M31R32R33I34R352345-2635R81G82L83A84V85K862036-2293Cre recombinaseCre re...inase2,2802,3002,3202,3402,3602,3802,4002,4202,440
    AGGAGAACGTTGATGCTGGAGAGCGTGCTAAGCAAGCTCTTGCTTTCGAGCGTACCGATTTCGATCAAGTTCGTTCTCTTATGGAGAACTCTGATCGCTGCCAAGATATCCGTAACCTTGCTTTCCTTGGAATCGCTTACAACACCCTTCTTCGTATCGCTGAGATCGCTCGTATTCCTCTTGCAACTACGACCTCTCGCACGATTCGTTCGAGAACGAAAGCTCGCATGGCTAAAGCTAGTTCAAGCAAGAGAATACCTCTTGAGACTAGCGACGGTTCTATAGGCATTGGAACGAAAGGAACCTTAGCGAATGTTGTGGGAAGAAGCATAGCGACTCTAGCGAGCATABlpIStyIBsaBIEcoRVK36E37N38V39D40A41G42E43R44A45K46Q47A48L49A50F51E52R53T54D55F56D57Q58V59R60S61L62M63E64N65S66D67R68C69Q70D71I72R73N74L75A76F77L78G79I80A81Y82N83T84L85L86R87I88A89E90I91A92R93I942345-2635Cre recombinase2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CCGTGTTAAGgtaagtttaaacagttcggtactaactaaccatacatatttaaattttcagGATATCTCTCGCACCGATGGAGGACGCATGCTTATCCACATCGGACGCACTAAGACCCTTGTTTCCACCGCTGGAGTTGAGAAGGCCCTTTCCCTTGGAGTTACCAAGCTTGTTGGCACAATTCcattcaaatttgtcaagccatgattgattggtatgtataaatttaaaagtcCTATAGAGAGCGTGGCTACCTCCTGCGTACGAATAGGTGTAGCCTGCGTGATTCTGGGAACAAAGGTGGCGACCTCAACTCTTCCGGGAAAGGGAACCTCAATGGTTCGAACAASphIStyIEcoRVEcoO109IR95V96K97Cre recombinaseCr...e2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GAGCGTTGGATCTCTGTTTCCGGAGTCGCTGATGATCCAAACAACTACCTTTTCTGTCGTGTTCGTAAGgtaagtttaaacatgattttactaactaactaatctgatttaaattttcagAACGGAGTTGCTGCTCCATCCGCTACCTCTCAACTTTCCACCCGTGCTCTTGAGGCTCGCAACCTAGAGACAAAGGCCTCAGCGACTACTAGGTTTGTTGATGGAAAAGACAGCACAAGCATTCcattcaaatttgtactaaaatgattgattgattagactaaatttaaaagtcTTGCCTCAACGACGAGGTAGGCGATGGAGAGTTGAAAGGTGGGCACGAGAACTCCBspEICre recombinaseCre recombinase2,8202,8402,8602,8802,9002,9202,9402,960
    GAATCTTCGAGGCTACTCACCGTCTTATCTACGGAGCTAAGGATGATTCCGGACAACGTTACCTTGCTTGGTCTGGACACTCCGCTCGTGTTGGAGCTGCTCGTGACATGGCTCGTGCTGGAGTTTCTATCCCAGAAATCATGCAGGCTGGAGGATGGACTAACGTTAACATTGTCTTAGAAGCTCCGATGAGTGGCAGAATAGATGCCTCGATTCCTACTAAGGCCTGTTGCAATGGAACGAACCAGACCTGTGAGGCGAGCACAACCTCGACGAGCACTGTACCGAGCACGACCTCAAAGATAGGGTCTTTAGTACGTCCGACCTCCTACCTGATTGCAATTGTAACAHpaIBspEIBpu10ICre recombinase2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TATGAACTACATCCGCAACCTTGATTCCGAGACCGGAGCTATGGTTCGTCTTCTTGAGGACGGAGACTAAACTAGTgataaatgcaaaatcctttcaagcattcccttcttctctatcactcttctttctttttgtcaaaaaattctctcgctaatttatttgcttttttaatgtATACTTGATGTAGGCGTTGGAACTAAGGCTCTGGCCTCGATACCAAGCAGAAGAACTCCTGCCTCTGATTTGATCActatttacgttttaggaaagttcgtaagggaagaagagatagtgagaagaaagaaaaacagttttttaagagagcgattaaataaacgaaaaaattacaAloIBbsIBsmISpeIBsaXItbb-2 3'UTRCre recombinase3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tattattttatgactttttatagtcactgaaaagtttgcatctgagtgaagtgaatgctatcaaaatgtgattctgtctgatgtactttcacaatctctcttcaattccattttgaagtgctttaaacccgaaaggttgagaaaaatgcgagcgctcaaatatttgtattgtgttataataaaatactgaaaaatatcagtgacttttcaaacgtagactcacttcacttacgatagttttacactaagacagactacatgaaagtgttagagagaagttaaggtaaaacttcacgaaatttgggctttccaactctttttacgctcgcgagtttataaacataacacaaAfeIBsmItbb-2 3'UTR3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cgttgagtgacccaacaaaaagaggaaactttattgtgccgccaagaaaaaagtctcacgggtaccgagctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatgcaactcactgggttgtttttctcctttgaaataacacggcggttcttttttcagagtgcccatggctcgagcttaagtgaccggcagcaaaatgttgcagcactgacccttttgggaccgcaatgggttgaattagcggaacgtcgtgtagggggaaagcggtcgaccgcattaBmrIKpnISacIBanIIEcoRIAcc65IEco53kItbb-2 3'UTRM13/pUC ForwardM13 ForwardM13 fwd3,5203,5403,5603,5803,6003,6203,6403,660
    agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgctcgcttctccgggcgtggctagcgggaagggttgtcaacgcgtcggacttaccgcttaccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcgBglIFspIKasINarINdeIPvuISfoIPluTIBstAPIpRS-marker3,6803,7003,7203,7403,7603,7803,8003,8203,840
    caacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataatgttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattaPfoIBsmBIBsmBIEsp3IEsp3IEcoO109IpGEX 3'pBRforEco3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccttaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcggZraIAatIIAmpR promoterAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactAcuIXmnIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    gcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattgBcgIScaIAmp-RAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    catgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttacPvuIAvaIIAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcgcgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgcBglIFspIAvaIIBsrDIBsrFINmeAIIIAmpR4,7404,7604,7804,8004,8204,8404,8604,880
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    4897 bp3500375040004250450047502505007501000lac promoteroripBR322ori-FAmp-RAmpRZCS241 (DNA)ZCS249 (DNA)ZCS238 (DNA)ZCS242 (DNA)ZCS243 (DNA)ZCS157 (DNA)ZCS235 (DNA)ZCS150 (DNA)ZCS237 (DNA)MS120 (DNA)M13F (DNA)MS387 (DNA)

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    4897