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pT2Apuro-Stargazin-mEGFP-iLID
  • Sequence Map
Editing disabled on read-only sequence.
CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGBspHI20406080100120140160
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCAmpR180200220240260280300320340
TATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGAAmpR360380400420440460480500520
GACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATFspIPvuIAmpR540560580600620640660680700
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAmpR720740760780800820840860880
TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAhdIAmpR9009209409609801,0001,0201,0401,060
TTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATBspHIColE1 origin1,0801,1001,1201,1401,1601,1801,2001,2201,240
CCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCAlwNIColE1 origin1,2601,2801,3001,3201,3401,3601,3801,4001,420
GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCColE1 origin1,4401,4601,4801,5001,5201,5401,5601,5801,600
CGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGDrdIColE1 origin1,6201,6401,6601,6801,7001,7201,7401,7601,780
TTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGSapIBspQI1,8001,8201,8401,8601,8801,9001,9201,940
GTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTlac promoterLacO1,9601,9802,0002,0202,0402,0602,0802,1002,120
CAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGgagctcGGATCTGCTGGGCTTGCTGAAGGTAGGGGGtcaagaacCAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTATTATTTTTGGGGATTTTTACTTTGTCGATACTGGTACTAATGCGGTTCGCGCGTTAATTGGGAGTGATTTCCCTTGTTTTCGACctcgagCCTAGACGACCCGAACGACTTCCATCCCCCagttcttgGTCTCCACATTTCATGAACTCATTAAAATGAACTAATGACATGAATTCATAATAAAAACCCCTAAAAATGAAAAflIITol2L200T3T3 promoterM13-rev2,1402,1602,1802,2002,2202,2402,2602,2802,300
ACTTGAGTACAATTAAAAATCAATACTTTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTCCTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTTgggccctctagatgcatgctcgaCATTGATTATTGactagttattaatagtTGAACTCATGTTAATTTTTAGTTATGAAAATGAAAATGAATTAATGTAAAAAAATCTTTTTTTTCATGAAAAATGAGGAATGTTAAAATAAATGTCAGTTTTTCATGAATAAAAAACCTCTAGTGAAcccgggagatctacgtacgagctGTAACTAATAACtgatcaataattatcaNsiISpeISphITol2L2002,3202,3402,3602,3802,4002,4202,4402,4602,480
aatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcatCMV enhancer2,5002,5202,5402,5602,5802,6002,6202,6402,660
tttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgagaaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccagctcNcoINdeISnaBICMV enhancer2,6802,7002,7202,7402,7602,7802,8002,8202,840
gtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtccgccccgccccgccccgctccccgccccgccccgctccgcctctccacgccChicken beta-actin promoter2,8602,8802,9002,9202,9402,9602,9803,0003,020
cggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcggggagtcgctgcgacgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcggccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgcccctcagcgacgctgcgacggaagcggggcacggggcgaggcggcggcggagcgcggcgggcggggccgagactgactggcgcChicken beta-actin promoterchicken beta-actin intron3,0403,0603,0803,1003,1203,1403,1603,1803,200
ttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggaaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgaacaaagaaaagacaccgacgcactttcggaactccccgaggccctcccgggaaacacgcccccctcgccgagccccccacgcacgcacacacacacgcacccctAfeIchicken beta-actin intron3,2203,2403,2603,2803,3003,3203,3403,3603,380
gcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagccgcggcgcacgccgaggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgtcacacgcgctcccctcgcgccggcccccgccacggggcgccacgccccccccgacgctccccttgtttccgacgcacgccccacacacgcacccccccactcgAfeIchicken beta-actin intron3,4003,4203,4403,4603,4803,5003,5203,5403,560
agggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggcctcccccacacccgcgcagccagcccgacgttgggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcatgccccgcaccgcgccccgagcggcacggcccgccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggBsiWIchicken beta-actin intron3,5803,6003,6203,6403,6603,6803,7003,720
ggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggccccctcccgagccccctccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgMreISgrAIBstAPIchicken beta-actin intron3,7403,7603,7803,8003,8203,8403,8603,8803,900
gcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctccgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcccccctgccgacggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagchicken beta-actin intron3,9203,9403,9603,9804,0004,0204,0404,0604,080
tagagcctctgctaaccatgttcatgccttcttctttttcctacagCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAgaattGGGATCCGAATTCaccATGGGGCTGTTTGATCGAGGTGTTCAAATGCTTTTAACCACCGTTGGTGCTTTCGCTGCCTTCatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcGAGGACCCGTTGCACGACCAATAACACGACAGAGTAGTAAAACCGTTTcttaaCCCTAGGCTTAAGtggTACCCCGACAAACTAGCTCCACAAGTTTACGAAAATTGGTGGCAACCACGAAAGCGACGGAAGNcoIAlwNIBamHIMG2LF4DR6GV8QM10LL12TT14VG16AF18AA20F4210-5220StargazinCAG16404,1004,1204,1404,1604,1804,2004,2204,2404,260
AGCTTGATGACCATCGCTGTGGGAACCGACTATTGGCTGTACTCCAGAGGGGTTTGCAAGACCAAAAGTGTCAGTGAGAATGAAACCAGCAAGAAGAACGAGGAAGTTATGACCCATTCCGGATTATGGAGAACCTGCTGCCTCGAAGGGAACTTCAAAGGTCTGTGCAAGCAAATCGTCGAACTACTGGTAGCGACACCCTTGGCTGATAACCGACATGAGGTCTCCCCAAACGTTCTGGTTTTCACAGTCACTCTTACTTTGGTCGTTCTTCTTGCTCCTTCAATACTGGGTAAGGCCTAATACCTCTTGGACGACGGAGCTTCCCTTGAAGTTTCCAGACACGTTCGTTTAGCAjuIBstXIS22LM24TI26AV28GT30DY32WL34YS36RG38VC40KT42KS44VS46EN48ET50SK52KN54EE56VM58TH60SG62LW64RT66CC68LE70GN72FK74GL76CK78QI804210-5220Stargazin4,2804,3004,3204,3404,3604,3804,4004,4204,440
ACCACTTTCCGGAAGACGCGGACTACGAAGCTGACACCGCAGAGTATTTCCTCCGGGCCGTGAGGGCCTCGAGTATCTTCCCGATCCTGAGTGTGATCCTGCTTTTCATGGGTGGCCTCTGCATCGCAGCCAGCGAGTTCTACAAACCGGTGAACTGCAGGAAGAGCTCGAAGAATTCTGGTGAAAGGCCTTCTGCGCCTGATGCTTCGACTGTGGCGTCTCATAAAGGAGGCCCGGCACTCCCGGAGCTCATAGAAGGGCTAGGACTCACACTAGGACGAAAAGTACCCACCGGAGACGTAGCGTCGGTCGCTCAAGATGTTTGGCCACTTGACGTCCTTCTCGAGCTTCTTAAGAgeISapISfiITliIXhoIBspQIBstBIPspXIPaeR7IDH82FP84ED86AD88YE90AD92TA94EY96FL98RA100VR102AS104SI106FP108IL110SV112IL114LF116MG118GL120CI122AA124SE126FY128KP130VN132CR134KS136SK138NS1404210-5220StargazinStargazin4,4604,4804,5004,5204,5404,5604,5804,6004,620
GAAAAGCTTGACACGCCACAACATCATCCTGAGTGCTGGCATCTTCTTCGTGTCTGCAGGTCTTAGTAATATCATCGGGATCATCGTGTATATATCAGCCAATGCTGGAGACCCCTCCAAGAGTGACTCCAAAAAGAACAGCTACTCCTACGGCTGGTCCTTCTACTTCGGGGCCCTGCTTTTCGAACTGTGCGGTGTTGTAGTAGGACTCACGACCGTAGAAGAAGCACAGACGTCCAGAATCATTATAGTAGCCCTAGTAGCACATATATAGTCGGTTACGACCTCTGGGGAGGTTCTCACTGAGGTTTTTCTTGTCGATGAGGATGCCGACCAGGAAGATGAAGCCCCGGGACAloIKS142LT144RH146NI148IL150SA152GI154FF156VS158AG160LS162NI164IG166II168VY170IS172AN174AG176DP178SK180SD182SK184KN186SY188SY190GW192SF194YF196GA198L4210-5220Stargazin4,6404,6604,6804,7004,7204,7404,7604,7804,800
TCCTTCATCATCGCCGAGATGGTCGGGGTGCTGGCCGTGCACATGTTTATCGACCGCCACAAACAGCTGCGGGCCACGGCCCGCGCCACCGACTACCTCCAGGCCTCCGCCATCACCCGCATCCCCAGCTACCGCTACCGCTACCAGCGCCGCAGCCGCTCCAGCTCGCGCTCCACCGAGGAAGTAGTAGCGGCTCTACCAGCCCCACGACCGGCACGTGTACAAATAGCTGGCGGTGTTTGTCGACGCCCGGTGCCGGGCGCGGTGGCTGATGGAGGTCCGGAGGCGGTAGTGGGCGTAGGGGTCGATGGCGATGGCGATGGTCGCGGCGTCGGCGAGGTCGAGCGCGAGGTGGCStuIS200FI202IA204EM206VG208VL210AV212HM214FI216DR218HK220QL222RA224TA226RA228TD230YL232QA234SA236IT238RI240PS242YR244YR246YQ248RR250SR252SS254SR256ST2584210-5220Stargazin4,8204,8404,8604,8804,9004,9204,9404,9604,980
AGCCCTCTCACTCCAGAGACGCCTCGCCCGTGGGCGTGAAGGGCTTCAACACCCTGCCGTCCACGGAGATCTCCATGTACACCCTCAGTAGGGACCCCCTGAAGGCTGCCACCACGCCCACCGCCACCTACAACTCGGACAGGGATAACAGCTTTCTCCAGGTCCACAACTGTATCCATCGGGAGAGTGAGGTCTCTGCGGAGCGGGCACCCGCACTTCCCGAAGTTGTGGGACGGCAGGTGCCTCTAGAGGTACATGTGGGAGTCATCCCTGGGGGACTTCCGACGGTGGTGCGGGTGGCGGTGGATGTTGAGCCTGTCCCTATTGTCGAAAGAGGTCCAGGTGTTGACATAGGTBaeIKflIBglIIBsmBIBsrGIEsp3IPpuMIEP260SH262SR264DA266SP268VG270VK272GF274NT276LP278ST280EI282SM284YT286LS288RD290PL292KA294AT296TP298TA300TY302NS304DR306DN308SF310LQ312VH314NC316IQ3184210-5220Stargazin5,0005,0205,0405,0605,0805,1005,1205,1405,160
GAAGGACAGCAAGGACTCTcTCCACGCCAACACAGCCAACCGCCGGACCACGCCCGTAgaattcggcATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATCTTCCTGTCGTTCCTGAGAgAGGTGCGGTTGTGTCGGTTGGCGGCCTGGTGCGGGCATcttaagccgTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTAKD320SK322DS324LH326AN328TA330NR332RT334TP336V4210-5220EF2GM4VS6KG8EE10LF12TG14VV16PI18LV20EL22DG24DV26NG28HK30FS32VS34GE36GE38GD405221-6648mEGFPStargazin5,1805,2005,2205,2405,2605,2805,3005,3205,340
GCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCCGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGAT42YG44KL46TL48KF50IC52TT54GK56LP58VP60WP62TL64VT66TL68TY70GV72QC74FS76RY78PD80HM82KQ84HD86FF88KS90AM92PE94GY96VQ98E5221-6648mEGFP5,3605,3805,4005,4205,4405,4605,4805,500
GCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTR100TI102FF104KD106DG108NY110KT112RA114EV116KF118EG120DT122LV124NR126IE128LK130GI132DF134KE136DG138NI140LG142HK144LE146YN148YN150SH152NV154YI156MA158D5221-6648mEGFP5,5205,5405,5605,5805,6005,6205,6405,6605,680
CAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTTAGCAAAGACCCCAACGAGAAGGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGTTCGAATCGTTTCTGGGGTTGCTCTTCBpu10IK160QK162NG164IK166VN168FK170IR172HN174IE176DG178SV180QL182AD184HY186QQ188NT190PI192GD194GP196VL198LP200DN202HY204LS206TQ208SK210LS212KD214PN216EK2185221-6648mEGFP5,7005,7205,7405,7605,7805,8005,8205,8405,860
CGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACctcgagGGTGGATCTGgaggttcaggtggaagtCTGGCAACCACACTGGAACGGATCGAGAAAAATTTCGTGATTACTGATCCGAGACTGCCTGACAACCCAATCATTTGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGgagctcCCACCTAGACctccaagtccaccttcaGACCGTTGGTGTGACCTTGCCTAGCTCTTTTTAAAGCACTAATGACTAGGCTCTGACGGACTGTTGGGTTAGTAAATliIXhoIPspXIPaeR7IRD220HM222VL224LE226FV228TA230AG232IT234LG236MD238EL240YL242EG244GS246GG248SG250GS252LA254TT256LE258RI260EK262NF264VI266TD268PR270LP272DN274PI276I5221-6648iLidmEGFPlinkerlinker5,8805,9005,9205,9405,9605,9806,0006,0206,040
TTGCGAGCGATTCCTTCCTGCAGCTGACAGAATATTCTCGGGAAGAGATCCTGGGGCGCAATTGCCGTTTTCTGCAGGGACCCGAGACAGACCGTGCCACTGTTCGGAAAATCAGAGATGCTATTGACAACCAGACTGAAGTGACCGTTCAGCTGATCAATTATACCAAGAGCGGCAAAACGCTCGCTAAGGAAGGACGTCGACTGTCTTATAAGAGCCCTTCTCTAGGACCCCGCGTTAACGGCAAAAGACGTCCCTGGGCTCTGTCTGGCACGGTGACAAGCCTTTTAGTCTCTACGATAACTGTTGGTCTGACTTCACTGGCAAGTCGACTAGTTAATATGGTTCTCGCCGTTBclIKflIMfeIPpuMIF278AS280DS282FL284QL286TE288YS290RE292EI294LG296RN298CR300FL302QG304PE306TD308RA310TV312RK314IR316DA318ID320NQ322TE324VT326VQ328LI330NY332TK334SG336K5221-6648iLid6,0606,0806,1006,1206,1406,1606,1806,2006,220
GAAGTTCTGGAACGTGTTCCACCTGCAGCCGATGCGCGATTATAAGGGCGACGTCCAGTACTTCATTGGCGTGCAGCTGGATGGCACCGAACGTCTTCATGGCGCCGCTGAGCGTGAGGCGGTCTGCCTGATCAAAAAGACAGCCTTTCAGATTGCTGAGGCAGCGAACGACGAAAATCTTCAAGACCTTGCACAAGGTGGACGTCGGCTACGCGCTAATATTCCCGCTGCAGGTCATGAAGTAACCGCACGTCGACCTACCGTGGCTTGCAGAAGTACCGCGGCGACTCGCACTCCGCCAGACGGACTAGTTTTTCTGTCGGAAAGTCTAACGACTCCGTCGCTTGCTGCTTTTABclIXcmIBbvCIBpu10IBstAPIK338FW340NV342FH344LQ346PM348RD350YK352GD354VQ356YF358IG360VQ362LD364GT366ER368LH370GA372AE374RE376AV378CL380IK382KT384AF386QI388AE390AA392ND394EN3965221-6648iLid6,2406,2606,2806,3006,3206,3406,3606,3806,400
TACTTTtaaGCGGCCGCTCTAGAgtcgacGTTAACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGGGATCAATTCCGCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATATGAAAattCGCCGGCGAGATCTcagctgCAATTGATTGAATTCGATCGTTGCCAAAGGGAGATCGCCCTAGTTAAGGCGGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTAAccIBmtIHpaINheINotISalIAflIIYF398*A400AA402LE404ST406LT408NL410S*412QR414FP416SS418GI420NS422AP424PP426*R428YW430PK432PL434GI436RP438VC440VC442LY444VI446FH448HI450AV452FW454Q5221-6648IRES6,4206,4406,4606,4806,5006,5206,5406,5606,580
GTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGCACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACAvrIIC456EG458PE460TW462PC464LL466DE468HS470*G472SF474PS4765221-6648IRES6,6006,6206,6406,6606,6806,7006,7206,7406,760
GCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGACGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTKpnIPmlIPflMIAcc65IDraIIIIRES6,7806,8006,8206,8406,8606,8806,9006,9206,940
TCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCaGGGCCGTACGCACCCTCAGACCCCGGAGCCACGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTATTATGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGtCCCGGCATGCGTGGGAGPasIBmgBIBsiWIPflFITth111IIRESPuro_R6,9606,9807,0007,0207,0407,0607,0807,1007,120
GCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGAtCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTaGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCRsrIIBstEIIPuro_R7,1407,1607,1807,2007,2207,2407,2607,280
AAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGMscIStuIBsmBIEsp3IPuro_R7,3007,3207,3407,3607,3807,4007,4207,4407,460
CGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAggaGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCACGGACTcctBamHISexAIBsu36IPuro_R7,4807,5007,5207,5407,5607,5807,6007,6207,640
tccAATggaggccgcCACCGCGGTGGAGCTCGAATTAATTCATCGATGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAaggTTAcctccggcgGTGGCGCCACCTCGAGCTTAATTAAGTAGCTACTACTAGGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTAATATTCGACGTTATTClaIBspDIBstXISV40 late polyA7,6607,6807,7007,7207,7407,7607,7807,8007,820
ACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCagatctTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACAATTTTAATGGATGTTCAATTGTTGTTGTTAACGTAAGTAAAATACAAAGTCCAAGTCCCCCTCCACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATACCGACTAATACTAGGAGATCTAGtctagaAAGAACGAAAATGAAAATGAAGGAATTATGAGTTCATGTTAAAATTACCTHpaIMfeIBglIISV40 late polyATol2R1757,8407,8607,8807,9007,9207,9407,9607,9808,000
GTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGATACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCACTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTGtcaagaaccatatgCCggtaccCAATTCGCCCTATAGTGAGTCGTATTACGCGCATGAAAAAATGAAAATGAGTTCATTCTAAGATCGGTCTATGAAAATGAAAATTAACTCATTTTAAAAGGGATTCATGAACATGAAAGTGAACTCATTTTAAAAACTCATGAAAAATGTGGAGACagttcttggtatacGGccatggGTTAAGCGGGATATCACTCAGCATAATGCGCKpnINdeIAcc65ITol2R175T78,0208,0408,0608,0808,1008,1208,1408,1608,180
CGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCCTGCGCGGGACATCGCFspIPvuILacZ alphaM13-fwd8,2008,2208,2408,2608,2808,3008,3208,3408,360
GCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTF1 ori8,3808,4008,4208,4408,4608,4808,5008,5208,540
CCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACAloIDrdIDraIIIF1 ori8,5608,5808,6008,6208,6408,6608,6808,7008,720
CCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAATCCACCCspCI8,7408,7608,7808,800
  • Plasmid
Editing disabled on read-only sequence.
8813 bp65007000750080008500500100015002000T3T3 promoter5221-6648IRES-hyg-F (DNA)IRES-hyg-R (DNA)BspHIAmpR,PvuI,FspIAhdIBspHIAlwNIColE1 originDrdIBspQISapIlac promoter,LacO,M13-revAvrIIIRES,PmlI,DraIIIAcc65I,KpnI,PflMIBmgBI,PflFI,Tth111I,PasIBsiWI,RsrII,BstEIIPuro_RStuI,MscI,BsmBI,Esp3ISexAI,Bsu36I,BamHI,BstXISV40 late polyA,BspDI,ClaI,HpaI,MfeITol2R175,BglIIT7,M13-fwd,LacZ alpha,NdeI,Acc65I,KpnI,PvuI,FspIF1 ori,DraIII,DrdI,AloI,CspCI

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Enzyme AatII
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8813