Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pT2Apuro-Stargazin-mEGFP-iLID
  • Sequence Map
    Editing disabled on read-only sequence.
    CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAABspHI20406080100120140160
    TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACAmpR180200220240260280300320340
    GCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTAmpR360380400420440460480500520
    CTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATFspIPvuIAmpR540560580600620640660680700
    ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACAmpR720740760780800820840860
    GTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCAhdIAmpR8809009209409609801,0001,0201,040
    GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAABspHIColE1 origin1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTAlwNIColE1 origin1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCColE1 origin1,4201,4401,4601,4801,5001,5201,5401,560
    GAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGDrdIColE1 origin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCSapIBspQICol...in1,7601,7801,8001,8201,8401,8601,8801,9001,920
    AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACAClac promoter1,9401,9601,9802,0002,0202,0402,0602,0802,100
    TGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGgagctcGGATCTGCTGGGCTTGCTGAAGGTAGGGGGtcaagaacCAGAGGTGTAAAGTACTTGAGTAATTTTACTTGAACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGCGGTTCGCGCGTTAATTGGGAGTGATTTCCCTTGTTTTCGACctcgagCCTAGACGACCCGAACGACTTCCATCCCCCagttcttgGTCTCCACATTTCATGAACTCATTAAAATGAACTTol2L200LacOM13-revT3T3 promoter2,1202,1402,1602,1802,2002,2202,2402,260
    TTACTGTACTTAAGTATTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACTTTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTCCTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTTgggccctctAATGACATGAATTCATAATAAAAACCCCTAAAAATGAAATGAACTCATGTTAATTTTTAGTTATGAAAATGAAAATGAATTAATGTAAAAAAATCTTTTTTTTCATGAAAAATGAGGAATGTTAAAATAAATGTCAGTTTTTCATGAATAAAAAACCTCTAGTGAAcccgggagaAflIITol2L2002,2802,3002,3202,3402,3602,3802,4002,4202,440
    agatgcatgctcgaCATTGATTATTGactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccattctacgtacgagctGTAACTAATAACtgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtaNsiISpeISphICMV enhancer2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttccttcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggaNdeICMV enhancer2,6402,6602,6802,7002,7202,7402,7602,7802,800
    acttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccagtgaaccgtcatgtagatgcataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtcNcoISnaBIChicken beta-actin promoterCMV enhancer2,8202,8402,8602,8802,9002,9202,9402,960
    gcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcggggagtcgctgcgacgctgccttcgcccccgccccgccccgccccgctccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgcccctcagcgacgctgcgacggaagcggggChicken beta-actin promoterchicken bet...tin intron2,9803,0003,0203,0403,0603,0803,1003,1203,140
    gtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggaggcacggggcgaggcggcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgaacaaagaaaagacaccgacgcactttcggaactccccgaggccctccAfeIchicken beta-actin intron3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggaaacacgcccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgaggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgtcacacgcgctcccctcgcgccggcccccgccacggggcgccacAfeIchicken beta-actin intron3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccccccccgacgctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgcagccagcccgacgttgggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcatgccccgcaccgcgccccgagBsiWIchicken beta-actin intron3,5203,5403,5603,5803,6003,6203,6403,660
    gccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggaccggcacggcccgccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgMreISgrAIBstAPIchicken beta-actin intron3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcgaaggaaacagggtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcchicken beta-actin intron3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAgaattGGGATCCccccctgccgacggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcGAGGACCCGTTGCACGACCAATAACACGACAGAGTAGTAAAACCGTTTcttaaCCCTAGGAlwNIBamHICAG16404,0404,0604,0804,1004,1204,1404,1604,1804,200
    GAATTCaccATGGGGCTGTTTGATCGAGGTGTTCAAATGCTTTTAACCACCGTTGGTGCTTTCGCTGCCTTCAGCTTGATGACCATCGCTGTGGGAACCGACTATTGGCTGTACTCCAGAGGGGTTTGCAAGACCAAAAGTGTCAGTGAGAATGAAACCAGCAAGAAGAACGAGGCTTAAGtggTACCCCGACAAACTAGCTCCACAAGTTTACGAAAATTGGTGGCAACCACGAAAGCGACGGAAGTCGAACTACTGGTAGCGACACCCTTGGCTGATAACCGACATGAGGTCTCCCCAAACGTTCTGGTTTTCACAGTCACTCTTACTTTGGTCGTTCTTCTTGCTCCAjuINcoIBstXIMG2LF4DR6GV8QM10LL12TT14VG16AF18AA20FS22LM24TI26AV28GT30DY32WL34YS36RG38VC40KT42KS44VS46EN48ET50SK52KN54E4210-5220Stargazin4,2204,2404,2604,2804,3004,3204,3404,360
    AAGTTATGACCCATTCCGGATTATGGAGAACCTGCTGCCTCGAAGGGAACTTCAAAGGTCTGTGCAAGCAAATCGACCACTTTCCGGAAGACGCGGACTACGAAGCTGACACCGCAGAGTATTTCCTCCGGGCCGTGAGGGCCTCGAGTATCTTCCCGATCCTGAGTGTGATCCTTTCAATACTGGGTAAGGCCTAATACCTCTTGGACGACGGAGCTTCCCTTGAAGTTTCCAGACACGTTCGTTTAGCTGGTGAAAGGCCTTCTGCGCCTGATGCTTCGACTGTGGCGTCTCATAAAGGAGGCCCGGCACTCCCGGAGCTCATAGAAGGGCTAGGACTCACACTAGGASfiITliIXhoIPspXIPaeR7IE56VM58TH60SG62LW64RT66CC68LE70GN72FK74GL76CK78QI80DH82FP84ED86AD88YE90AD92TA94EY96FL98RA100VR102AS104SI106FP108IL110SV112IL1144210-5220Stargazin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GCTTTTCATGGGTGGCCTCTGCATCGCAGCCAGCGAGTTCTACAAACCGGTGAACTGCAGGAAGAGCTCGAAGAATTCGAAAAGCTTGACACGCCACAACATCATCCTGAGTGCTGGCATCTTCTTCGTGTCTGCAGGTCTTAGTAATATCATCGGGATCATCGTGTATATATCACGAAAAGTACCCACCGGAGACGTAGCGTCGGTCGCTCAAGATGTTTGGCCACTTGACGTCCTTCTCGAGCTTCTTAAGCTTTTCGAACTGTGCGGTGTTGTAGTAGGACTCACGACCGTAGAAGAAGCACAGACGTCCAGAATCATTATAGTAGCCCTAGTAGCACATATATAGTAgeISapIBspQIBstBILF116MG118GL120CI122AA124SE126FY128KP130VN132CR134KS136SK138NS140KS142LT144RH146NI148IL150SA152GI154FF156VS158AG160LS162NI164IG166II168VY170IS1724210-5220StargazinStargazin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    GCCAATGCTGGAGACCCCTCCAAGAGTGACTCCAAAAAGAACAGCTACTCCTACGGCTGGTCCTTCTACTTCGGGGCCCTGTCCTTCATCATCGCCGAGATGGTCGGGGTGCTGGCCGTGCACATGTTTATCGACCGCCACAAACAGCTGCGGGCCACGGCCCGCGCCACCGACTCGGTTACGACCTCTGGGGAGGTTCTCACTGAGGTTTTTCTTGTCGATGAGGATGCCGACCAGGAAGATGAAGCCCCGGGACAGGAAGTAGTAGCGGCTCTACCAGCCCCACGACCGGCACGTGTACAAATAGCTGGCGGTGTTTGTCGACGCCCGGTGCCGGGCGCGGTGGCTGAAloIAN174AG176DP178SK180SD182SK184KN186SY188SY190GW192SF194YF196GA198LS200FI202IA204EM206VG208VL210AV212HM214FI216DR218HK220QL222RA224TA226RA228TD2304210-5220Stargazin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ACCTCCAGGCCTCCGCCATCACCCGCATCCCCAGCTACCGCTACCGCTACCAGCGCCGCAGCCGCTCCAGCTCGCGCTCCACCGAGCCCTCTCACTCCAGAGACGCCTCGCCCGTGGGCGTGAAGGGCTTCAACACCCTGCCGTCCACGGAGATCTCCATGTACACCCTCAGTAGTGGAGGTCCGGAGGCGGTAGTGGGCGTAGGGGTCGATGGCGATGGCGATGGTCGCGGCGTCGGCGAGGTCGAGCGCGAGGTGGCTCGGGAGAGTGAGGTCTCTGCGGAGCGGGCACCCGCACTTCCCGAAGTTGTGGGACGGCAGGTGCCTCTAGAGGTACATGTGGGAGTCATCStuIBglIIBsmBIBsrGIEsp3IYL232QA234SA236IT238RI240PS242YR244YR246YQ248RR250SR252SS254SR256ST258EP260SH262SR264DA266SP268VG270VK272GF274NT276LP278ST280EI282SM284YT286LS288R4210-5220Stargazin4,9204,9404,9604,9805,0005,0205,0405,060
    GGACCCCCTGAAGGCTGCCACCACGCCCACCGCCACCTACAACTCGGACAGGGATAACAGCTTTCTCCAGGTCCACAACTGTATCCAGAAGGACAGCAAGGACTCTcTCCACGCCAACACAGCCAACCGCCGGACCACGCCCGTAgaattcggcATGGTGAGCAAGGGCGAGGAGCCTGGGGGACTTCCGACGGTGGTGCGGGTGGCGGTGGATGTTGAGCCTGTCCCTATTGTCGAAAGAGGTCCAGGTGTTGACATAGGTCTTCCTGTCGTTCCTGAGAgAGGTGCGGTTGTGTCGGTTGGCGGCCTGGTGCGGGCATcttaagccgTACCACTCGTTCCCGCTCCTCBaeIKflIPpuMID290PL292KA294AT296TP298TA300TY302NS304DR306DN308SF310LQ312VH314NC316IQ318KD320SK322DS324LH326AN328TA330NR332RT334TP336V4210-5220EF2GM4VS6KG8EE105221-6648StargazinmEGFP5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGAGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACTLF12TG14VV16PI18LV20EL22DG24DV26NG28HK30FS32VS34GE36GE38GD40AT42YG44KL46TL48KF50IC52TT54GK56LP58VP60WP62TL64VT66TL685221-6648mEGFP5,2605,2805,3005,3205,3405,3605,3805,4005,420
    CCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTATY70GV72QC74FS76RY78PD80HM82KQ84HD86FF88KS90AM92PE94GY96VQ98ER100TI102FF104KD106DG108NY110KT112RA114EV116KF118EG120DT122LV124NR126I5221-6648mEGFP5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGE128LK130GI132DF134KE136DG138NI140LG142HK144LE146YN148YN150SH152NV154YI156MA158DK160QK162NG164IK166VN168FK170IR172HN174IE176DG178SV180QL182AD184H5221-6648mEGFP5,6205,6405,6605,6805,7005,7205,7405,760
    TACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTTAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACctcgagGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGTTCGAATCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGgagctcCTliIXhoIPspXIBpu10IPaeR7IY186QQ188NT190PI192GD194GP196VL198LP200DN202HY204LS206TQ208SK210LS212KD214PN216EK218RD220HM222VL224LE226FV228TA230AG232IT234LG236MD238EL240YL242E5221-6648mEGFP5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GTGGATCTGgaggttcaggtggaagtCTGGCAACCACACTGGAACGGATCGAGAAAAATTTCGTGATTACTGATCCGAGACTGCCTGACAACCCAATCATTTTTGCGAGCGATTCCTTCCTGCAGCTGACAGAATATTCTCGGGAAGAGATCCTGGGGCGCAATTGCCGTTTTCTCACCTAGACctccaagtccaccttcaGACCGTTGGTGTGACCTTGCCTAGCTCTTTTTAAAGCACTAATGACTAGGCTCTGACGGACTGTTGGGTTAGTAAAAACGCTCGCTAAGGAAGGACGTCGACTGTCTTATAAGAGCCCTTCTCTAGGACCCCGCGTTAACGGCAAAAGAMfeIG244GS246GG248SG250GS252LA254TT256LE258RI260EK262NF264VI266TD268PR270LP272DN274PI276IF278AS280DS282FL284QL286TE288YS290RE292EI294LG296RN298CR300FL3025221-6648iLidlinkerlinker5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GCAGGGACCCGAGACAGACCGTGCCACTGTTCGGAAAATCAGAGATGCTATTGACAACCAGACTGAAGTGACCGTTCAGCTGATCAATTATACCAAGAGCGGCAAGAAGTTCTGGAACGTGTTCCACCTGCAGCCGATGCGCGATTATAAGGGCGACGTCCAGTACTTCATTGGCCGTCCCTGGGCTCTGTCTGGCACGGTGACAAGCCTTTTAGTCTCTACGATAACTGTTGGTCTGACTTCACTGGCAAGTCGACTAGTTAATATGGTTCTCGCCGTTCTTCAAGACCTTGCACAAGGTGGACGTCGGCTACGCGCTAATATTCCCGCTGCAGGTCATGAAGTAACCGBclIKflIXcmIPpuMIBstAPIQG304PE306TD308RA310TV312RK314IR316DA318ID320NQ322TE324VT326VQ328LI330NY332TK334SG336KK338FW340NV342FH344LQ346PM348RD350YK352GD354VQ356YF358IG3605221-6648iLid6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GTGCAGCTGGATGGCACCGAACGTCTTCATGGCGCCGCTGAGCGTGAGGCGGTCTGCCTGATCAAAAAGACAGCCTTTCAGATTGCTGAGGCAGCGAACGACGAAAATTACTTTtaaGCGGCCGCTCTAGAgtcgacGTTAACTAACTTAAGCTAGCAACGGTTTCCCTCTAGCGCACGTCGACCTACCGTGGCTTGCAGAAGTACCGCGGCGACTCGCACTCCGCCAGACGGACTAGTTTTTCTGTCGGAAAGTCTAACGACTCCGTCGCTTGCTGCTTTTAATGAAAattCGCCGGCGAGATCTcagctgCAATTGATTGAATTCGATCGTTGCCAAAGGGAGATCGCAccIBclIBmtIHpaINheINotISalIAflIIBbvCIBpu10IVQ362LD364GT366ER368LH370GA372AE374RE376AV378CL380IK382KT384AF386QI388AE390AA392ND394EN396YF398*A400AA402LE404ST406LT408NL410S*412QR414FP416SS4185221-6648iLidIRES6,3206,3406,3606,3806,4006,4206,4406,460
    GGATCAATTCCGCCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCTAGTTAAGGCGGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCAvrIIGI420NS422AP424PP426*R428YW430PK432PL434GI436RP438VC440VC442LY444VI446FH448HI450AV452FW454QC456EG458PE460TW462PC464LL466DE468HS470*G472SF474PS4765221-6648IRES6,4806,5006,5206,5406,5606,5806,6006,6206,640
    CCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTPmlIIRES6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCACGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTGCAGATCKpnIPflMIAcc65IDraIIIIRES6,8406,8606,8806,9006,9206,9406,9606,9807,000
    GCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCaGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGAtCCGGACCGCCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTATTATGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGtCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTaGGCCTGGCGGPasIBmgBIBsiWIPflFIRsrIITth111IPuro_RIRES7,0207,0407,0607,0807,1007,1207,1407,160
    ACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCBstEIIPuro_R7,1807,2007,2207,2407,2607,2807,3007,3207,340
    TTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGMscIStuIBsmBIEsp3IPuro_R7,3607,3807,4007,4207,4407,4607,4807,5007,520
    TTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGTGCCCGAAGGACCGCGCGACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAggatccAATggaggccgcCACCGCGGTGGAGCTCGAATTAATTCATCGAAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCACGGACTcctaggTTAcctccggcgGTGGCGCCACCTCGAGCTTAATTAAGTAGCTClaIBamHIBspDIBstXISexAIBsu36IPuro_R7,5407,5607,5807,6007,6207,6407,6607,6807,700
    TGATGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTACTACTAGGTCTGTACTATTCTATGTAACTACTCAAACCTGTTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAACGAAATAAACATTGGTAATATTCGACGTTATTTGTTCAATTGTTGTTGTTAACGTAAGTAAAATACAAAGTCCAAHpaIMfeISV40 late polyA7,7207,7407,7607,7807,8007,8207,8407,860
    CAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCagatctTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACAATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGATGTCCCCCTCCACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGTTTACACCATACCGACTAATACTAGGAGATCTAGtctagaAAGAACGAAAATGAAAATGAAGGAATTATGAGTTCATGTTAAAATTACCTCATGAAAAAATGAAAATGAGTTCATTCTAAGATCGGTCTABglIITol2R175SV40 late polyA7,8807,9007,9207,9407,9607,9808,0008,0208,040
    ACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCACTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTGtcaagaaccatatgCCggtaccCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTGAAAATGAAAATTAACTCATTTTAAAAGGGATTCATGAACATGAAAGTGAACTCATTTTAAAAACTCATGAAAAATGTGGAGACagttcttggtatacGGccatggGTTAAGCGGGATATCACTCAGCATAATGCGCGCGAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCKpnINdeIAcc65ITol2R175T7M13-fwd8,0608,0808,1008,1208,1408,1608,1808,2008,220
    AAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCFspIPvuILacZ alphaF1 ori8,2408,2608,2808,3008,3208,3408,3608,3808,400
    CAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTAF1 ori8,4208,4408,4608,4808,5008,5208,5408,560
    GGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGCCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACAloIDrdIDraIIIF1 ori8,5808,6008,6208,6408,6608,6808,7008,7208,740
    AGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAATCCACCCspCI8,7608,7808,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8813 bp65007000750080008500500100015002000T3T3 promoter5221-6648IRES-hyg-F (DNA)IRES-hyg-R (DNA)

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    8813