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SEP4_Ozempic Plasmid
  • Sequence Map
    Editing disabled on read-only sequence.
    ctagaaataattttgtttaactttaagaaggagatatacatatgcaccatcatcatcatcatATGGCAGGTGCTCCGGGCCCTCTACGTCTGGCGCTGTTGCTTTTGGGTATGGTGGGACGCGCCGGCCCAAGACCTCAGGGAGCCACTGTGTCACTTTGGGAAACGGTGCAGAAgatctttattaaaacaaattgaaattcttcctctatatgtatacgtggtagtagtagtagtaTACCGTCCACGAGGCCCGGGAGATGCAGACCGCGACAACGAAAACCCATACCACCCTGCGCGGCCGGGTTCTGGAGTCCCTCGGTGACACAGTGAAACCCTTTGCCACGTCTTApaINdeINdeIBfuAIBspMIPaqCIBsu36IPspOMIMA2GA4PG6PL8RL10AL12LL14LG16MV18GR20AG22PR24PQ26GA28TV30SL32WE34TV36QK3863-1451H1H2H3H4H5H66xHisGLP-1 (Ozempic)efficien..., 1989)source20406080100120140160
    ATGGCGCGAATATCGTCGTCAGTGCCAACGGAGCCTTACCGAAGACCCGCCCCCGGCCACTGACCTGTTCTGCAACCGGACCTTCGATGAGTATGCCTGCTGGCCCGATGGTGAGCCTGGTTCCTTTGTTAACGTGAGTTGCCCGTGGTACCTGCCGTGGGCGTCGTCGGTCCCGTACCGCGCTTATAGCAGCAGTCACGGTTGCCTCGGAATGGCTTCTGGGCGGGGGCCGGTGACTGGACAAGACGTTGGCCTGGAAGCTACTCATACGGACGACCGGGCTACCACTCGGACCAAGGAAACAATTGCACTCAACGGGCACCATGGACGGCACCCGCAGCAGCCAGGGCHpaIKpnIBfuAIBspMIAcc65IWR40EY42RR44QC46QR48SL50TE52DP54PP56AT58DL60FC62NR64TF66DE68YA70CW72PD74GE76PG78SF80VN82VS84CP86WY88LP90WA92SS94VP9663-1451GLP-1 (Ozempic)source180200220240260280300320340
    CAGGGCCACGTTTATCGTTTTTGCACGGCTGAAGGTTTGTGGCTGCAGAAAGATAATAGCTCCTTACCCTGGCGTGATCTGTCGGAATGTGAGGAATCCAAACGCGGAGAACGATCCTCGCCGGAAGAGCAGCTACTGTTCCTGTATATTATTTATACTGTTGGCTATGCGCTCAGTCCCGGTGCAAATAGCAAAAACGTGCCGACTTCCAAACACCGACGTCTTTCTATTATCGAGGAATGGGACCGCACTAGACAGCCTTACACTCCTTAGGTTTGCGCCTCTTGCTAGGAGCGGCCTTCTCGTCGATGACAAGGACATATAATAAATATGACAACCGATACGCGAGTBlpIPstISapIAlwNIBspQIQG98HV100YR102FC104TA106EG108LW110LQ112KD114NS116SL118PW120RD122LS124EC126EE128SK130RG132ER134SS136PE138EQ140LL142FL144YI146IY148TV150GY152AL15463-1451GLP-1 (Ozempic)source360380400420440460480500520
    GCTTTTCAGCACTGGTCATAGCGAGCGCCATTCTTTTAGGTTTTCGTCATCTTCACTGCACACGCAATTATATCCACCTGAATCTGTTCGCTAGCTTTATTCTGCGCGCCCTGTCGGTGTTCATCAAGGACGCGGCGCTGAAGTGGATGTACTCGACCGCGGCACAGCAGCATCACGAAAAGTCGTGACCAGTATCGCTCGCGGTAAGAAAATCCAAAAGCAGTAGAAGTGACGTGTGCGTTAATATAGGTGGACTTAGACAAGCGATCGAAATAAGACGCGCGGGACAGCCACAAGTAGTTCCTGCGCCGCGACTTCACCTACATGAGCTGGCGCCGTGTCGTCGTAGTBmtINheISacIIBssHIISF156SA158LV160IA162SA164IL166LG168FR170HL172HC174TR176NY178IH180LN182LF184AS186FI188LR190AL192SV194FI196KD198AA200LK202WM204YS206TA208AQ210QH212Q63-1451GLP-1 (Ozempic)source540560580600620640660680700
    ATGGGATGGCCTTCTGTCATATCAGGATTCGTTAAGTTGCCGCCTTGTGTTCCTCCTGATGCAATATTGTGTGGCAGCGAATTACTATTGGCTCCTGGTGGAAGGCGTCTACTTGTACACCCTCTTGGCATTCAGCGTTTTAAGCGAACAGTGGATTTTCCGTCTCTACGTTAGTTACCCTACCGGAAGACAGTATAGTCCTAAGCAATTCAACGGCGGAACACAAGGAGGACTACGTTATAACACACCGTCGCTTAATGATAACCGAGGACCACCTTCCGCAGATGAACATGTGGGAGAACCGTAAGTCGCAAAATTCGCTTGTCACCTAAAAGGCAGAGATGCAATCABsrGIW214DG216LL218SY220QD222SL224SC226RL228VF230LL232MQ234YC236VA238AN240YY242WL244LV246EG248VY250LY252TL254LA256FS258VL260SE262QW264IF266RL268YV270S63-1451GLP-1 (Ozempic)source720740760780800820840860
    ATTGGGTGGGGCGTCCCGCTTTTATTTGTAGTGCCATGGGGAATTGTCAAATACCTGTACGAAGATGAAGGCTGCTGGACTCGCAATAGCAACATGAACTACTGGTTAATTATTCGCTTACCTATTCTGTTTGCCATCGGGGTGAACTTTCTGATCTTTGTGCGTGTTATCTGTATAACCCACCCCGCAGGGCGAAAATAAACATCACGGTACCCCTTAACAGTTTATGGACATGCTTCTACTTCCGACGACCTGAGCGTTATCGTTGTACTTGATGACCAATTAATAAGCGAATGGATAAGACAAACGGTAGCCCCACTTGAAAGACTAGAAACACGCACAATAGACATNcoII272GW274GV276PL278LF280VV282PW284GI286VK288YL290YE292DE294GC296WT298RN300SN302MN304YW306LI308IR310LP312IL314FA316IG318VN320FL322IF324VR326VI328C63-1451GLP-1 (Ozempic)source8809009209409609801,0001,0201,040
    TTGTAGTATCCAAACTGAAAGCGAACTTAATGTGTAAAACCGATATAAAATGCCGCCTGGCCAAAAGCACCCTGACGTTGATTCCGCTGTTAGGGACACATGAGGTTATCTTCGCGTTCGTAATGGATGAGCACGCACGCGGCACCTTGCGCTTTATCAAACTTTTTACCGAACTAACATCATAGGTTTGACTTTCGCTTGAATTACACATTTTGGCTATATTTTACGGCGGACCGGTTTTCGTGGGACTGCAACTAAGGCGACAATCCCTGTGTACTCCAATAGAAGCGCAAGCATTACCTACTCGTGCGTGCGCCGTGGAACGCGAAATAGTTTGAAAAATGGCTTGAMscII330VV332SK334LK336AN338LM340CK342TD344IK346CR348LA350KS352TL354TL356IP358LL360GT362HE364VI366FA368FV370MD372EH374AR376GT378LR380FI382KL384FT386EL38863-1451GLP-1 (Ozempic)source1,0601,0801,1001,1201,1401,1601,1801,2001,220
    GAGCTTTACGTCTTTTCAAGGTCTCATGGTAGCTATCCTGTACTGTTTTGTGAACAATGAAGTCCAACTAGAATTCCGCAAGAGCTGGGAGCGTTGGCGACTGGAACATTTGCATATTCAGCGTGACTCAAGTATGAAGCCGCTGAAATGCCCAACCTCATCTCTGTCTTCTGGCCTCGAAATGCAGAAAAGTTCCAGAGTACCATCGATAGGACATGACAAAACACTTGTTACTTCAGGTTGATCTTAAGGCGTTCTCGACCCTCGCAACCGCTGACCTTGTAAACGTATAAGTCGCACTGAGTTCATACTTCGGCGACTTTACGGGTTGGAGTAGAGACAGAAGACCGEcoRISF390TS392FQ394GL396MV398AI400LY402CF404VN406NE408VQ410LE412FR414KS416WE418RW420RL422EH424LH426IQ428RD430SS432MK434PL436KC438PT440SS442LS444SG44663-1451GLP-1 (Ozempic)source1,2401,2601,2801,3001,3201,3401,3601,3801,400
    GCAACAGCCGGCAGTTCAATGTATACCGCGACGTGTCAGGCGAGCTGTAGCTAATAGTAAgtaccatggagaccgacgtcaaacgcaaaagaaaatgccgatatcctattggcattgacggtctccagtaaaggtggatacggatccgaattcgagctccgtcgacaagcttgcgCGTTGTCGGCCGTCAAGTTACATATGGCGCTGCACAGTCCGCTCGACATCGATTATCATTcatggtacctctggctgcagtttgcgttttcttttacggctataggataaccgtaactgccagaggtcatttccacctatgcctaggcttaagctcgaggcagctgttcgaacgcEagINcoINotISacISalIZraIAatIIBamHIBmgBIEcoRIEcoRVPshAIBstZ17IEco53kIHindIIIAT448AG450SS452MY454TA456TC458QA460SC462S63-1451*1*2*3GLP-1 (Ozempic)source1,4201,4401,4601,4801,5001,5201,5401,560
    gccgcactcgaggagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagccggcgtgagctcctctaggccgacgattgtttcgggctttccttcgactcaaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctaaccgcttaccctgcgcgggacatcgAvaIBlpITliIXhoIBseRIBsoBIBspEIPspXIPaeR7IT7 terminatorT7 Termf1 orisource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgf1 oriF1ori-Rsource1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataaggccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccAloIPsiIBsaAIDraIIIf1 oriF1ori-Fsource1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgactaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactCspCIf1 orisource2,1202,1402,1602,1802,2002,2202,2402,260
    attaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaataattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggttPflMI*272FF270ED268LM266LH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228QY226RD224AI222GV220RG218VKanRsource2,2802,3002,3202,3402,3602,3802,4002,4202,440
    catcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggNsiID216IC214GI212LK210GE208DF206IL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED168DF166DS164AD162VL160GKanRsource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatccaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagAjuIAvaIPvuISmaIXmaIAsiSIBsoBIEcoNITspMIBpu10IN158NM156RS154QA152QA150LR148FV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP102IKanRsource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatccgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagClaINsiIBspDIA100TT98LL96WA94DD92PT90RI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY44LR42KanRsource2,8202,8402,8602,8802,9002,9202,9402,960
    gatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgactatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactNruIYI40TA38GS36QG34VN32DR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2MKanRKan-RpENTR-Rsource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggorisource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcacttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagtAlwNIorisource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    agacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccorisource3,5203,5403,5603,5803,6003,6203,6403,660
    tcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacBssSIBssSαIoripBR322ori-Fsource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctggaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacPciISapIBspQIbomL4440source3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tgcggtatttcacaccgcaatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacacgccataaagtgtggcgttaccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgBsaAIPflFIBstZ17ITth111IbompRS-markersource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtcttctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaPfoI*64L63N62E61G60D59D58G57F56R55A54L53C52S51R50Y49L48E47D46A45H44D43H42L41S40E39C38I37D36A35Q34E33D32A31D30L29E28N27L26K25E24L23L22T21L20T19Q18roppGEX 3'source4,2204,2404,2604,2804,3004,3204,3404,360
    ggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccS17R16I15F14R13A12M11N10L9A8T7K6E5Q4K3T2V1ropsource4,3804,4004,4204,4404,4604,4804,5004,5204,540
    taaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccagttacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgcttBspEIsource4,5604,5804,6004,6204,6404,6604,6804,7004,720
    acacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgtgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccccggcFspIFspAIPpuMIBpu10Isource4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacBglIsource4,9204,9404,9604,9805,0005,0205,0405,060
    tcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttcaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaagPshAI*Q360GS358ElacIsource5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggttgtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaL356RS354VQ352RA350LQ348ML346SD344AL342AR340PS338AT336QT334NP332AL330TT328KR326KV324LS322VP320LL318QN316GK314VA312QG310QS308LQ306LL304RD302VS300TlacIsource5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaagccatagcagcatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcHpaIEcoRVBssHIIQ298GL296LR294FD292QK290IT288TL286PP284IY282CS280SD278ET276DD274YG272VV270SI268DA266GV264RL262GS260ET258IA256RM254AG252LA250MQ248DN246AV244LM242AT240lacIsource5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaApaIPspOMINmeAIIIPV238IG236EN234LM232QM230TQ228QF226GS224MA222SW220DG218ER216EA214IP212QI210QN208RT206LY204KH202WG200AL198RL196RA194SV192SS190LP188GA186LL184AI182lacIsource5,6205,6405,6605,6805,7005,7205,7405,760
    ttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattacBclIBstEIIQQ180HG178LA176VL174HE172VG170LR168TG166DE164HS162FI160IS158NI156PT154QD152SV150DL148FL146AP144VN142TC140AA138EV136AI134AD132QD130DL128PY126NI124lacIsource5,7805,8005,8205,8405,8605,8805,9005,9205,940
    atcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgctagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcgMluIIL122GS120VR118QA116LL114NH112VA110AK108CA106EV104GS102RE100VM98SV96VV94SA92GL90QD88AR86SK84IA82AV80IQ78SP76AH74LA72LS70ST68AV66GlacIsource5,9605,9806,0006,0206,0406,0606,0806,1006,120
    caatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtagttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatBstAPII64LL62SQ60KG58AL56QQ54AV52RN50PI48YN46LE44AM42AA40EV38KE36RT34KA32SV30HS28AQ26NV24VR22SV20TQ18YS16VG14AY12EA10VD8YlacILacI-Rsource6,1406,1606,1806,2006,2206,2406,2606,2806,300
    taacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgcattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcgPfoIEcoNIPflMIL6TV4PK2VlacIlacI promotersource6,3206,3406,3606,3806,4006,4206,4406,460
    cgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccacSphISgrAIpBRrevBamsource6,4806,5006,5206,5406,5606,5806,6006,6206,640
    atgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactatagggtacggccggtgctacgcaggccgcatctcctagctctagagctagggcgctttaattatgctgagtgatatcccBglIIT7 promotersourceT76,6606,6806,7006,720
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6724 bp50005500600050010001500T7 Termsource6xHislacI63-14511452-1460+14PstI,BspQI,SapI,AlwNI,BlpIGLP-1 (Ozempic),NheI,BmtI,BssHII,SacIIBsrGINcoIMscIEcoRIBstZ17I,BmgBI,NcoI,PshAI,ZraIAatII,EcoRV,BamHI,EcoRI,Eco53kI,SacI,SalI,HindIII,EagI,NotIAvaIT7 terminator,BsoBI,PaeR7I,PspXI,TliI,XhoI,BseRI,BlpIPshAIHpaIEcoRV,BssHIIPspOMI,ApaI,NmeAIII,BstEIIBclIMluILacI-R,lacI promoter,BstAPIpBRrevBam,T7 promoter,T7,PflMI,PfoI,EcoNI,SphI,SgrAI,BglII

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    6724