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pdf0432-huDisCas7-11-R978-GGGS-R1294-U6-pro-Gluc
  • Sequence Map
    Editing disabled on read-only sequence.
    cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaPciISapIBspQIL4440source20406080100120140160
    ggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagDrdIBciVIoripBR322ori-Fsource180200220240260280300320340
    ccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccacggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgApaLIorisource360380400420440460480500520
    tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtAlwNIorisource540560580600620640660680700
    aaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctagorisource720740760780800820840860
    cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttacgaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatgAmpRsource8809009209409609801,0001,0201,040
    catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtgtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaBpmIBsaINmeAIIIAmpRsource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcFspIAmpRsource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcgBcgIAmpRAmp-Rsource1,4201,4401,4601,4801,5001,5201,5401,560
    cacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcagtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgtApaLIAmpRsource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgtttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacSspIBciVIAmpR promoterAmpRsource1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaaggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttAloIPsiISspICspCIf1 oriF1ori-Fsource1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagagcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctDrdIDraIIIf1 orisource2,1202,1402,1602,1802,2002,2202,2402,260
    aaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctatttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataFspIf1 oriF1ori-Rsource2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tacgccagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctacatgcggtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggaacatcaattactaattgggcggtacgatgaatagatgAAV2 ITRsource2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtagccatgctctaggaagagtaccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcaGTGGTTTGTCTGGTCAACtaggtcttgaaaggacatcggtacgagatccttctcatggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtCACCAAACAGACCAGTTGatccagaactttcctSnaBIsourceAttP seq Bxb1source2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgatccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccacccttaaccgaggccacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaactaggccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttgEcoNIsourceEF-1α core promotersource2,8202,8402,8602,8802,9002,9202,9402,960
    cgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggGCCACATGACGACTACTATGAAGATTTCAATTGAATTCCTCGAGCCGTTTCGGATGACCAAATGGCAGGAAAGCACTAGGCGAAACAAGAACAACAAGGAATTTGTTCGgcatatattcacgtcatcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtccCGGTGTACTGCTGATGATACTTCTAAAGTTAACTTAAGGAGCTCGGCAAAGCCTACTGGTTTACCGTCCTTTCGTGATCCGCTTTGTTCTTGTTGTTCCTTAAACAAGCBsgIsourcepiece 1DsiGRAMPTranslation 918-4787sourceEF-1α core promotersource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CGGCCAAGCTTTTGCAAGATGGCATAGAAATAAGAAGGATAACACAAAAGGGCGGCCTTACATAACTGGGACATTGCTGCGGTCCGCAGTAATTAGAAGCGCGGAAAACCTGCTGACATTGAGTGATGGGAAAATTAGCGAAAAGACATGTTGTCCTGGGAAATTTGATACGGAAGCCGGTTCGAAAACGTTCTACCGTATCTTTATTCTTCCTATTGTGTTTTCCCGCCGGAATGTATTGACCCTGTAACGACGCCAGGCGTCATTAATCTTCGCGCCTTTTGGACGACTGTAACTCACTACCCTTTTAATCGCTTTTCTGTACAACAGGACCCTTTAAACTATGCCTTPciIRsrIIHindIIIsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GATAAAGACAGGTTGCTGCAATTGAGGCAGCGGTCCACCCTTCGTTGGACCGATAAGAACCCCTGTCCAGATAACGCTGAAACTTACTGCCCGTTTTGCGAACTTCTCGGGAGATCCGGTAACGATGGAAAGAAGGCTGAGAAGAAGGATTGGCGCTTTCGTATTCATTTTGGCACTATTTCTGTCCAACGACGTTAACTCCGTCGCCAGGTGGGAAGCAACCTGGCTATTCTTGGGGACAGGTCTATTGCGACTTTGAATGACGGGCAAAACGCTTGAAGAGCCCTCTAGGCCATTGCTACCTTTCTTCCGACTCTTCTTCCTAACCGCGAAAGCATAAGTAAAACCGTsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ATCTTTCCCTTCCAGGGAAGCCCGATTTCGACGGGCCAAAAGCTATAGGCAGCCAACGGGTACTTAACAGGGTCGATTTCAAATCCGGTAAGGCCCATGACTTCTTTAAGGCTTACGAAGTCGACCATACTAGGTTTCCCCGCTTCGAAGGGGAGATTACCATAGATAATAAAGTTAGAAAGGGAAGGTCCCTTCGGGCTAAAGCTGCCCGGTTTTCGATATCCGTCGGTTGCCCATGAATTGTCCCAGCTAAAGTTTAGGCCATTCCGGGTACTGAAGAAATTCCGAATGCTTCAGCTGGTATGATCCAAAGGGGCGAAGCTTCCCCTCTAATGGTATCTATTATTTCASalIsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource3,5203,5403,5603,5803,6003,6203,6403,660
    CAGCGCTGAAGCCAGGAAACTGCTCTGTGATTCTCTCAAGTTTACGGATCGGCTGTGTGGAGCTCTGTGCGTAATCAGGTTCGATGAATATACACCAGCAGCCGATAGTGGGAAACAAACCGAAAATGTCCAAGCAGAACCGAATGCTAATCTCGCTGAAAAGACCGCAGAGCAAGTCGCGACTTCGGTCCTTTGACGAGACACTAAGAGAGTTCAAATGCCTAGCCGACACACCTCGAGACACGCATTAGTCCAAGCTACTTATATGTGGTCGTCGGCTATCACCCTTTGTTTGGCTTTTACAGGTTCGTCTTGGCTTACGATTAGAGCGACTTTTCTGGCGTCTCGTTAfeISacIEco53kIsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATTATTAGCATTCTGGACGATAATAAGAAAACCGAATATACCCGCCTGCTTGCTGATGCTATTCGTTCACTCCGCCGCTCTAGTAAACTTGTCGCTGGCTTGCCAAAGGATCATGACGGGAAGGATGACCATTACCTCTGGGATATCGGCAAGAAGAAGAAAGACGAAAACTCTGTAATAATCGTAAGACCTGCTATTATTCTTTTGGCTTATATGGGCGGACGAACGACTACGATAAGCAAGTGAGGCGGCGAGATCATTTGAACAGCGACCGAACGGTTTCCTAGTACTGCCCTTCCTACTGGTAATGGAGACCCTATAGCCGTTCTTCTTCTTTCTGCTTTTGAGACEcoRVsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource3,8603,8803,9003,9203,9403,9603,9804,0004,020
    TCACTATTAGGCAAATCCTTACGACCTCAGCAGACACCAAGGAACTCAAGAATGCCGGAAAATGGAGAGAATTCTGCGAGAAGCTGGGTGAAGCGCTGTACCTCAAGAGTAAAGACATGAGTGGCGGCCTGAAAATTACTCGGAGAATACTGGGCGATGCTGAATTCCATGGAAAAGTGATAATCCGTTTAGGAATGCTGGAGTCGTCTGTGGTTCCTTGAGTTCTTACGGCCTTTTACCTCTCTTAAGACGCTCTTCGACCCACTTCGCGACATGGAGTTCTCATTTCTGTACTCACCGCCGGACTTTTAATGAGCCTCTTATGACCCGCTACGACTTAAGGTACCTTTAfeINcoIBbvCIBpu10Isourcepiece 1DsiGRAMPTranslation 918-4787sourcesource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCCCGATCGGCTTGAAAAGAGCCGGTCTGTGTCAATCGGATCTGTGTTGAAAGAAACTGTGGTATGCGGCGAACTGGTCGCTAAAACGCCCTTCTTCTTCGGAGCGATAGACGAAGATGCAAAACAAACCgacCTGCAAGTACTCCTCACTCCCGATAACAAGTATAGACTGCCACGGGCTAGCCGAACTTTTCTCGGCCAGACACAGTTAGCCTAGACACAACTTTCTTTGACACCATACGCCGCTTGACCAGCGATTTTGCGGGAAGAAGAAGCCTCGCTATCTGCTTCTACGTTTTGTTTGGctgGACGTTCATGAGGAGTGAGGGCTATTGTTCATATCTGACGGTsourcepiece 1DsiGRAMPTranslation 918-4787sourcesource4,2204,2404,2604,2804,3004,3204,3404,360
    AGAAGCGCCGTGCGAGGTATACTCCGTCGAGATCTTCAAACCTATTTTGATAGCCCATGCAATGCTGAGTTGGGTGGACGGCCATGCATGTGTAAGACGTGTAGGATTATGAGAGGGATCACGGTGATGGATGCGCGCAGTGAGTACAATGCCCCGCCAGAAATAAGGCATCGTATCTTCGCGGCACGCTCCATATGAGGCAGCTCTAGAAGTTTGGATAAAACTATCGGGTACGTTACGACTCAACCCACCTGCCGGTACGTACACATTCTGCACATCCTAATACTCTCCCTAGTGCCACTACCTACGCGCGTCACTCATGTTACGGGGCGGTCTTTATTCCGTAGCATNsiIBglIIBmgBIBstZ17Isourcepiece 1DsiGRAMPTranslation 918-4787sourcesource4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CCCGCATTAATCCCTTCACAGGCACGGTTGCGGAAGGTGCCCTGTTTAATATGGAAGTAGCCCCCGAGGGGATTGTCTTTCCATTTCAACTCCGGTACCGGGGCTCTGAAGATGGGCTGCCCGATGCACTGAAAACGGTGTTGAAATGGTGGGCTGAGGGGCAGGCATTCATGAGGGGCGTAATTAGGGAAGTGTCCGTGCCAACGCCTTCCACGGGACAAATTATACCTTCATCGGGGGCTCCCCTAACAGAAAGGTAAAGTTGAGGCCATGGCCCCGAGACTTCTACCCGACGGGCTACGTGACTTTTGCCACAACTTTACCACCCGACTCCCCGTCCGTAAGTACTCBaeIKpnIBbvCIAcc65IBpu10Isourcepiece 1DsiGRAMPTranslation 918-4787sourcesource4,5604,5804,6004,6204,6404,6604,6804,7004,720
    TGGCGCTGCCTCAACCGGGAAGGGCCGATTCCGGATGGAAAATGCTAAATATGAAACGCTGGATCTGAGCGACGAGAATCAAAGGAATGACTATCTTAAGAATTGGGGATGGCGTGACGAGAAGGGGCTCGAGGAACTGAAGAAACGACTGAACTCAGGTCTGCCAGAGCCCGGTACCGCGACGGAGTTGGCCCTTCCCGGCTAAGGCCTACCTTTTACGATTTATACTTTGCGACCTAGACTCGCTGCTCTTAGTTTCCTTACTGATAGAATTCTTAACCCCTACCGCACTGCTCTTCCCCGAGCTCCTTGACTTCTTTGCTGACTTGAGTCCAGACGGTCTCGGGCCAAflIIBspEIsourceDsiGRAMPTranslation 918-4787sourcepiece 1piece 2source4,7404,7604,7804,8004,8204,8404,8604,8804,900
    AATTATAGGGATCCAAAATGGCACGAGATTAACGTTTCCATTGAGATGGCAAGCCCTTTTATTAATGGCGACCCAATCCGCGCAGCCGTGGACAAACGTGGTACAgatGTGGTTACCTTCGTTAAGTATAAAGCTGAAGGGGAAGAGGCGAAACCCGTATGTGCATACAAGGCCGTTAATATCCCTAGGTTTTACCGTGCTCTAATTGCAAAGGTAACTCTACCGTTCGGGAAAATAATTACCGCTGGGTTAGGCGCGTCGGCACCTGTTTGCACCATGTctaCACCAATGGAAGCAATTCATATTTCGACTTCCCCTTCTCCGCTTTGGGCATACACGTATGTTCCGGCBaeIBstEIIsourceDsiGRAMPTranslation 918-4787sourcepiece 2source4,9204,9404,9604,9805,0005,0205,0405,060
    AATCTTTTAGAGGGGTGATCAGAAGTGCCGTGGCACGCATTCATATGGAAGATGGCGTCCCTTTGACTGAGTTGACTCACAGTGACTGTGAATGTCTCCTGTGCCAAATCTTTGGAAGTGAGTATGAAGCCGGCAAAATAAGGTTTGAAGATCTCGTATTCGAAAGTGACCCGGATTAGAAAATCTCCCCACTAGTCTTCACGGCACCGTGCGTAAGTATACCTTCTACCGCAGGGAAACTGACTCAACTGAGTGTCACTGACACTTACAGAGGACACGGTTTAGAAACCTTCACTCATACTTCGGCCGTTTTATTCCAAACTTCTAGAGCATAAGCTTTCACTGGGCCTBclIAlwNIBglIIsourceDsiGRAMPTranslation 918-4787sourcepiece 2source5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ACCTGTGACCTTCGATCATGTGGCCATCGATAGATTCACTGGCGGTGCAGCTGATAAGAAGAAATTCGATGATTCCCCTCTGCCCGGTAGCCCTGCAAGACCGCTCATGTTGAAAGGCTCCTTCTGGATCCGCAGGGACGTTCTCGAGGACGAAGAGTACTGTAAGGCACTCGGTTGGACACTGGAAGCTAGTACACCGGTAGCTATCTAAGTGACCGCCACGTCGACTATTCTTCTTTAAGCTACTAAGGGGAGACGGGCCATCGGGACGTTCTGGCGAGTACAACTTTCCGAGGAAGACCTAGGCGTCCCTGCAAGAGCTCCTGCTTCTCATGACATTCCGTGAGCCABsgIClaIBspDIsourceDsiGRAMPTranslation 918-4787sourcepiece 2source5,2605,2805,3005,3205,3405,3605,3805,4005,420
    AAGGCTCTTGCAGATGTGAATAATGGCCTTTATCCCCTCGGTGGAAAGAGCGCCATCGGCTACGGACAGGTCAAGAGTCTGGGTATAAAGGGAGATGATAAGAGGATTTCTCGCCTCATGAATCCTGCCTTTGATGAGACAGATGTAGCCGTTCCAGAAAAGCCCAAAACTGATGTTCCGAGAACGTCTACACTTATTACCGGAAATAGGGGAGCCACCTTTCTCGCGGTAGCCGATGCCTGTCCAGTTCTCAGACCCATATTTCCCTCTACTATTCTCCTAAAGAGCGGAGTACTTAGGACGGAAACTACTCTGTCTACATCGGCAAGGTCTTTTCGGGTTTTGACTACsourceDsiGRAMPTranslation 918-4787sourcepiece 2source5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CCGAGGTTCGCATCGAGGCAGAGAAAGTATATTACCCACACTATTTCGTCGAACCCCATAAGAAGGTGGAACGCGAGGAGAAACCCTGTGGTCATCAAAAGTTCCACGAGGGGCGACTGACAGGTAAAATTCGGTGTAAGCTCATTACCAAGACACCCCTCATCGTCCCAGATACGGCTCCAAGCGTAGCTCCGTCTCTTTCATATAATGGGTGTGATAAAGCAGCTTGGGGTATTCTTCCACCTTGCGCTCCTCTTTGGGACACCAGTAGTTTTCAAGGTGCTCCCCGCTGACTGTCCATTTTAAGCCACATTCGAGTAATGGTTCTGTGGGGAGTAGCAGGGTCTATGSpeINmeAIIIsourceDsiGRAMPTranslation 918-4787sourcepiece 2source5,6205,6405,6605,6805,7005,7205,7405,760
    TAGTAATGACGATTTCTTCAGACCTGCGGATAAAGAAGCTCGGAAGGAAAAGGACGAATATCATAAATCATATGCTTTCTTCAGACTTCATAAACAAATCATGATTCCCGGGAGCGAATTGAGAGGAATGGTGAGTAGTGTCTACGAAACTGTGACAAATTCTTGCTTCAGGATAATCATTACTGCTAAAGAAGTCTGGACGCCTATTTCTTCGAGCCTTCCTTTTCCTGCTTATAGTATTTAGTATACGAAAGAAGTCTGAAGTATTTGTTTAGTACTAAGGGCCCTCGCTTAACTCTCCTTACCACTCATCACAGATGCTTTGACACTGTTTAAGAACGAAGTCCTATsourceDsiGRAMPTranslation 918-4787sourcepiece 2source5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TTTGATGAGACTAAACGGTTGTCATGGCGGgggggtgggtcaCGAACTGTAGACGATAGGATGATCGGCAAACGTATGAGCGCCGATCTGCGGCCGTGCCATGGAGATTGGGTGGAAGATGGTGATCTCAGTGCCTTGAATGCATATCCCGAGAAAAGACTCCTCTTGCGCCACCAAACTACTCTGATTTGCCAACAGTACCGCCcccccacccagtGCTTGACATCTGCTATCCTACTAGCCGTTTGCATACTCGCGGCTAGACGCCGGCACGGTACCTCTAACCCACCTTCTACCACTAGAGTCACGGAACTTACGTATAGGGCTCTTTTCTGAGGAGAACGCGGTGGEagINcoINsiIBsaBIsourceDsiGRAMPTranslation 918-4787sourcepiece 3piece 2source5,9605,9806,0006,0206,0406,0606,0806,1006,120
    CCAAAGGACTCTGCCCTGCTTGCCGGCTCTTTGGAACCGGATCTTACAAGGGCAGAGTCAGGTTTGGATTCGCGTCACTCGAAAACGATCCGGAGTGGCTGATCCCAGGCAAGAATCCCGGCGATCCGTTTCACGGCGGGCCGGTGATGCTCTCATTGTTGGAACGGCCTCGCCCGGTTTCCTGAGACGGGACGAACGGCCGAGAAACCTTGGCCTAGAATGTTCCCGTCTCAGTCCAAACCTAAGCGCAGTGAGCTTTTGCTAGGCCTCACCGACTAGGGTCCGTTCTTAGGGCCGCTAGGCAAAGTGCCGCCCGGCCACTACGAGAGTAACAACCTTGCCGGAGCGGGBspEIsourceDsiGRAMPTranslation 918-4787sourcepiece 3source6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GACTTGGAGTATACCGGGATCCGACAATAAGTTTAAAGTGCCTGGCAGAAAGTTTTACGTCCACCACCACGCCTGGAAAACCATTAAGGACGGGAACCATCCCACAACAGGCAAAGCTATTGAACAAAGCCCTAATAACCGCACTGTAGAAGCTCTCGCCGGCGGGAATTCCTTTCTGAACCTCATATGGCCCTAGGCTGTTATTCAAATTTCACGGACCGTCTTTCAAAATGCAGGTGGTGGTGCGGACCTTTTGGTAATTCCTGCCCTTGGTAGGGTGTTGTCCGTTTCGATAACTTGTTTCGGGATTATTGGCGTGACATCTTCGAGAGCGGCCGCCCTTAAGGAAAMreISgrAIBstZ17IsourceDsiGRAMPTranslation 918-4787sourcepiece 3source6,3206,3406,3606,3806,4006,4206,4406,460
    AGCTTCGAAATTGCCTTTGAGAACCTGAAAGAATGGGAGCTGGGTTTGCTCATCCACAGCCTGCAACTCGAAAAGGGTCTGGCGCATAAACTTGGAATGGCAAAGTCTATGGGATTTGGTTCAGTTGAAATTGACGTCGAATCAGTGCGCCTGAGAAAAGATTGGAAGCAATGGCTCGAAGCTTTAACGGAAACTCTTGGACTTTCTTACCCTCGACCCAAACGAGTAGGTGTCGGACGTTGAGCTTTTCCCAGACCGCGTATTTGAACCTTACCGTTTCAGATACCCTAAACCAAGTCAACTTTAACTGCAGCTTAGTCACGCGGACTCTTTTCTAACCTTCGTTACCGsourceDsiGRAMPTranslation 918-4787sourcepiece 3source6,4806,5006,5206,5406,5606,5806,6006,6206,640
    GGAATGGCAATTCCGAAATTCCCAACTGGTTGGGAAAAGGATTTGCTAAACTGAAGGAATGGTTCCGGGACGAGCTCGATTTTATAGAAAATCTTAAGAAACTTCTTTGGTTTCCTGAGGGCGACCAAGCACCCCGGGTTTGCTACCCCATGCTGCGAAAGAAGGACGATCCTAACCTTACCGTTAAGGCTTTAAGGGTTGACCAACCCTTTTCCTAAACGATTTGACTTCCTTACCAAGGCCCTGCTCGAGCTAAAATATCTTTTAGAATTCTTTGAAGAAACCAAAGGACTCCCGCTGGTTCGTGGGGCCCAAACGATGGGGTACGACGCTTTCTTCCTGCTAGGATTPfoISacIAflIIBsu36IEco53kIsourceDsiGRAMPTranslation 918-4787sourcepiece 3source6,6606,6806,7006,7206,7406,7606,7806,8006,820
    TGGGAATAGCGGTTACGAAGAACTCAAAGACGGGGAATTCAAGAAAGAAGATCGGCAGAAGAAGCTGACCACGCCGTGGACACCGTGGGCAtaaaataaaatatctttattttcattacaTCTGTGTGTTGGTTTTTTGTGTGgagggcctatttcccatgattccttcatatttACCCTTATCGCCAATGCTTCTTGAGTTTCTGCCCCTTAAGTTCTTTCTTCTAGCCGTCTTCTTCGACTGGTGCGGCACCTGTGGCACCCGTattttattttatagaaataaaagtaatgtAGACACACAACCAAAAAACACACctcccggataaagggtactaaggaagtataaaPflMIDraIIIEcoO109IsourcesourceDsiGRAMPTranslation 918-4787piece 3poly(A) signalU6 promotersource6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttccgtatatgctatgttccgacaatctctctattaatcttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagsourceU6 promotersource7,0207,0407,0607,0807,1007,1207,1407,160
    gatttcttggctttatatatcttgtggaaaggacgaaacaccgTTGatgtcacggaacTGCAGCCAGCTTTCCGGGCATTGGCTTCCATtttttttaagcttgggccgctcgaggggcccgggatcttcctagagcatggctacgtagataagtagcatggcgggttaatcattactaaagaaccgaaatatatagaacacctttcctgctttgtggcAACtacagtgccttgACGTCGGTCGAAAGGCCCGTAACCGAAGGTAaaaaaaattcgaacccggcgagctccccgggccctagaaggatctcgtaccgatgcatctattcatcgtaccgcccaattagtaatApaIPstISnaBIPspOMIHindIIIEcoO109Isourcemature DR crRNAU6 promotersourcesource7,1807,2007,2207,2407,2607,2807,3007,3207,340
    actacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcattaatgaatcggccaacgcgcggggagaggcggttgatgttccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacgtaattacttagccggttgcgcgcccctctccgccaAAV2 ITRsource7,3607,3807,4007,4207,4407,4607,4807,5007,520
    ttgcgtattgggcgctaacgcataacccgcgasource7,540
    • Plasmid
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    Editing disabled on read-only sequence.
    7541 bp550060006500700050010001500sourcesourceDsiGRAMPTranslation 918-4787AmpRAmp-Rpiece 3orisourcesource

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    7541