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LSM105 CMV-TO-CX3CR1-(noV2)-ADAR2dd(E488Q, T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggLSM044pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIAmp (bla)LSM044180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspINmeAIIIAmp (bla)LSM044360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)LSM044540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIXmnIAmp (bla)LSM044720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoterLSM0448809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoterLSM0441,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIBsiWILSM0441,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)LSM0441,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaNdeICMVCMV promoter (with TetOs)LSM0441,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggCspCISnaBICMVCMV promoter (with TetOs)LSM0441,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacIBsmBIEsp3ISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOLSM0441,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggaCTCTAGCGTTTAAACTTAAGCTTCGCCACCATGGATCAATTTCCAGAAAGTGTGACTGAAAATTTTGAGTATGACGACCTGGCAGAGGCCTGCTACATCGGCGATATTGTTGTCTTCGGCACTGTTTTCCTGAGCATTTTCTATTCCGTTATAgccctggctaggtcggaggcctGAGATCGCAAATTTGAATTCGAAGCGGTGGTACCTAGTTAAAGGTCTTTCACACTGACTTTTAAAACTCATACTGCTGGACCGTCTCCGGACGATGTAGCCGCTATAACAACAGAAGCCGTGACAAAAGGACTCGTAAAAGATAAGGCAATATPmeIStuIAflIIEcoNIHindIIIMD2QF4PE6SV8TE10NF12EY14DD16LA18EA20CY22IG24DI26VV28FG30TV32FL34SI36FY38SV40I2153-3242CX3CR1CMV pro...TetOs)LSM0442,1202,1402,1602,1802,2002,2202,2402,260
    TTCGCCATTGGCCTCGTTGGAAATCTTCTGGTGGTGTTCGCGCTCACCAATAGTAAGAAGCCTAAAAGCGTGACAGACATTTATCTTCTTAACCTCGCATTGTCAGACTTGTTATTTGTCGCCACCCTGCCATTTTGGACCCACTATTTAATTAATGAGAAGGGACTTCACAACGAAGCGGTAACCGGAGCAACCTTTAGAAGACCACCACAAGCGCGAGTGGTTATCATTCTTCGGATTTTCGCACTGTCTGTAAATAGAAGAATTGGAGCGTAACAGTCTGAACAATAAACAGCGGTGGGACGGTAAAACCTGGGTGATAAATTAATTACTCTTCCCTGAAGTGTTGCPacIF42AI44GL46VG48NL50LV52VF54AL56TN58SK60KP62KS64VT66DI68YL70LN72LA74LS76DL78LF80VA82TL84PF86WT88HY90LI92NE94KG96LH98N2153-3242CX3CR12,2802,3002,3202,3402,3602,3802,4002,4202,440
    CGATGTGTAAATTTACTACAGCCTTCTTCTTCATAGGGTTCTTTGGGTCAATCTTCTTTATAACAGTCATATCAATTGATAGATATCTGGCCATCGTTCTGGCTGCCAACAGCATGAATAATCGGACAGTGCAACATGGTGTAACTATTAGTCTGGGCGTATGGGCCGCCGCTATGCTACACATTTAAATGATGTCGGAAGAAGAAGTATCCCAAGAAACCCAGTTAGAAGAAATATTGTCAGTATAGTTAACTATCTATAGACCGGTAGCAAGACCGACGGTTGTCGTACTTATTAGCCTGTCACGTTGTACCACATTGATAATCAGACCCGCATACCCGGCGGCGATAMfeIMscIBstXIEcoRVA100MC102KF104TT106AF108FF110IG112FF114GS116IF118FI120TV122IS124ID126RY128LA130IV132LA134AN136SM138NN140RT142VQ144HG146VT148IS150LG152VW154AA156AI1582153-3242CX3CR12,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTTAGTGGCCGCTCCCCAATTCATGTTTACCAAACAAAAGGAAAACGAATGCTTGGGAGATTATCCAGAAGTACTGCAGGAAATTTGGCCCGTACTCAGAAACGTGGAGACAAACTTTCTTGGGTTTCTGTTGCCACTTCTGATTATGTCCTATTGCTACTTTAGGATAATCCAAAAATCACCGGCGAGGGGTTAAGTACAAATGGTTTGTTTTCCTTTTGCTTACGAACCCTCTAATAGGTCTTCATGACGTCCTTTAAACCGGGCATGAGTCTTTGCACCTCTGTTTGAAAGAACCCAAAGACAACGGTGAAGACTAATACAGGATAACGATGAAATCCTATTAGGTTBsmILV160AA162PQ164FM166FT168KQ170KE172NE174CL176GD178YP180EV182LQ184EI186WP188VL190RN192VE194TN196FL198GF200LL202PL204LI206MS208YC210YF212RI214IQ2162153-3242CX3CR12,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACCCTCTTTAGCTGTAAGAATCACAAGAAAGCCAAAGCTATCAAGCTCATATTACTGGTAGTAATTGTCTTCTTTCTCTTCTGGACGCCTTATAATGTCATGATTTTCTTGGAGACACTCAAGCTCTATGATTTCTTTCCATCTTGCGATATGCGCAAGGATTTGAGGCTTGCTCTGGGAGAAATCGACATTCTTAGTGTTCTTTCGGTTTCGATAGTTCGAGTATAATGACCATCATTAACAGAAGAAAGAGAAGACCTGCGGAATATTACAGTACTAAAAGAACCTCTGTGAGTTCGAGATACTAAAGAAAGGTAGAACGCTATACGCGTTCCTAAACTCCGAACGAGBplIFspITL218FS220CK222NH224KK226AK228AI230KL232IL234LV236VI238VF240FL242FW244TP246YN248VM250IF252LE254TL256KL258YD260FF262PS264CD266MR268KD270LR272LA2742153-3242CX3CR12,8202,8402,8602,8802,9002,9202,9402,960
    TGTCTGTAACAGAAACAGTTGCTTTCAGTCACTGCTGCTTAAACCCGTTGATCTATGCATTTGCCGGAGAAAAGTTCAGGCGTTATCTCTACCATCTTTATGGCAAGTGCCTTGCGGTGCTCTGCGGTCGGTCCGTCCATGTCGATTTCAGCTCCAGTGAAAGTCAAAGATCCAGACAGACATTGTCTTTGTCAACGAAAGTCAGTGACGACGAATTTGGGCAACTAGATACGTAAACGGCCTCTTTTCAAGTCCGCAATAGAGATGGTAGAAATACCGTTCACGGAACGCCACGAGACGCCAGCCAGGCAGGTACAGCTAAAGTCGAGGTCACTTTCAGTTTCTAGGTCBplINsiIBstXIRsrIILS276VT278ET280VA282FS284HC286CL288NP290LI292YA294FA296GE298KF300RR302YL304YH306LY308GK310CL312AV314LC316GR318SV320HV322DF324SS326SE328SQ330RS332R2153-3242CX3CR12,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACACGGGAGCGTTTTGAGTTCCAACTTCACTTACCACACCTCTGATGGTGATGCTCTGCTCCTGCTGGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGTGTGCCCTCGCAAAACTCAAGGTTGAAGTGAATGGTGTGGAGACTACCACTACGAGACGAGGACGACCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACAgeIKpnIXcmIAcc65IDQ2TP4SR6QP8IP10SE12GL14QL16HL18PQ20VL22AD24AV26SR28L3239-4524H334GS336VL338SS340NF342TY344HT346SD348GD350AL352LL354LG356TG358AL360HL362D2153-3242hADAR2 cat domainCX3CR1LSM0443,1603,1803,2003,2203,2403,2603,2803,3003,320
    GTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACBseRIBstEIIV30LG32KF34GD36LT38DN40FS42SP44HA46RR48KV50LA52GV54VM56TT58GT60DV62KD64AK66VI68SV70ST72GT74KC76IN78GE80YM82SD84RG86L3239-4524hADAR2 cat domainLSM0443,3403,3603,3803,4003,4203,4403,4603,4803,500
    CATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCBclIBsrGIA88LN90DC92HA94EI96IS98RR100SL102LR104FL106YT108QL110EL112YL114NN116KD118DQ120KR122SI124FQ126KS128ER130GG132FR134LK136EN138VQ140FH142LY144IS1463239-4524hADAR2 cat domainLSM0443,5203,5403,5603,5803,6003,6203,6403,660
    CACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACBcgIPfoITS148PC150GD152AR154IF156SP158HE160PI162LE164EP166AD168RH170PN172RK174AR176GQ178LR180TK182IE184SG186QG188TI190PV192RS194NA196SI198QA200WD202GV2043239-4524hADAR2 cat domainLSM0443,6803,7003,7203,7403,7603,7803,8003,8203,840
    CTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGAGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAvaIBlpITliIXhoIBamHIBsoBIPaeR7ILQ206GE208RL210LT212MS214CS216DK218IA220RW222NV224VG226IQ228GS230LL232SI234FV236EP238IY240FS242SI244IL246GS248LY250HG252DH254LS256RA258MY260QR2623239-4524hADAR2 cat domainLSM0443,8603,8803,9003,9203,9403,9603,9804,0004,020
    TCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCPpuMIIS264NI266ED268LP270PL272YT274LN276KP278LL280SG282IS284NA286EA288RQ290PG292KA294PN296FS298VN300WT302VG304DS306AI308EV310IN312AT314TG316KD318EL320G3239-4524hADAR2 cat domainLSM0444,0404,0604,0804,1004,1204,1404,1604,1804,200
    CCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGMluIR322AS324RL326CK328HA330LY332CR334WM336RV338HG340KV342PS344HL346LR348SK350IT352KP354NV356YH358ES360KL362AA364KE366YQ368AA370KA372RL374FT376AF378I3239-4524hADAR2 cat domainLSM0444,2204,2404,2604,2804,3004,3204,3404,360
    AAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgaTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPspXIPaeR7IPspOMIK380AG382LG384AW386VE388KP390TE392QD394QF396SL398TP400*A402AR404GG406PE408QK410LI412SE414ED416LN418SA420VD422HH424HH426HH4283239-4524*1hADAR2 cat domainc-myc epitopePolyhi...e tagLSM0444,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggagacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctBGH polyadenylation sequenceLSM0444,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtgcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccacClaINspISphIBspDIBGH polyadenylation sequenceBGH pALSM0444,7404,7604,7804,8004,8204,8404,8604,8804,900
    gttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattacaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatKflINaeIPpuMINgoMIVLSM0444,9204,9404,9604,9805,0005,0205,0405,060
    gggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagaBarIMscIXbaIBsmBIEsp3IPshAIHygromycinFRTLSM0445,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggEcoRIHygromycinLSM0445,2605,2805,3005,3205,3405,3605,3805,4005,420
    gccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtaNdeIPvuIAsiSIRsrIIBstAPIDraIIIHygromycinLSM0445,4405,4605,4805,5005,5205,5405,5605,5805,600
    atgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggctacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccgBstAPIDraIIINmeAIIIHygromycinLSM0445,6205,6405,6605,6805,7005,7205,7405,760
    cgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggAjuISacIIHygromycinLSM0445,7805,8005,8205,8405,8605,8805,9005,9205,940
    gcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcgHygromycinLSM0445,9605,9806,0006,0206,0406,0606,0806,1006,120
    cgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccacgctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtgNaeIPfoINgoMIVHygromycinLSM0446,1406,1606,1806,2006,2206,2406,2606,2806,300
    cccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtgggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccaBsmIBstZ17ISV40 pALSM0446,3206,3406,3606,3806,4006,4206,4406,460
    catagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatggtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacLSM0446,4806,5006,5206,5406,5606,5806,6006,6206,640
    aatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcattagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgtNspIPciISapIBspQILSM0446,6606,6806,7006,7206,7406,7606,7806,8006,820
    aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctctttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagagLSM044pUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaagacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattLSM044pUC ori7,0207,0407,0607,0807,1007,1207,1407,160
    ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtLSM044pUC ori7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttLSM044pUC ori7,3607,3807,4007,4207,4407,460
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7464 bp550060006500700050010001500LSM044HygromycinCMV promoter (with TetOs)CMVSV40 pAbla promoterAmp (bla)pUC oriEKo152.2 (DNA)FlpInF2 (DNA)EKo152.4 (DNA)NK164-RBPMS-A-for (DNA)XZp025.1 (DNA)oLSM0275 (DNA)YH029.1 (DNA)oLSM0299 (DNA)oLSM0287 (DNA)pNSK174 (DNA)oLSM0302 (DNA)XZp032.3 (DNA)oLSM0269 (DNA)XZp095.6 (DNA)EKo330.4 (DNA)oLSM0261 (DNA)pNSK053 (DNA)YXo033.4 (DNA)pNK018 (DNA)bghF (DNA)EKo326.3 (DNA)oLSM0086 (DNA)EKo152.1 (DNA)oLSM-pUC_ori_F (DNA)pUCoriR (DNA)AhdIBsaI,NmeAIIIFspI,AclIPvuIBcgIAclIXmnIoLSM0054 (DNA),oLSM0053 (DNA)SspISgrDIFlpInF1 (DNA)FlpInF (DNA),oLSM039 (DNA),MfeIBsiWIEKo279.1 (DNA),EKo272.1 (DNA),MluI,SpeIEK_CMVFa (DNA)EK_CMVFb (DNA)EK_CMVFc (DNA)NdeIEK_CMVFd (DNA),SnaBIEK_CMVFe (DNA)CspCINdeINmeAIIIBstAPI,DraIIIAjuISacIIHyg_F (DNA)PfoI,NgoMIV,NaeISV40PA_R (DNA),SV40pA-R Genewiz (DNA)BsmIBstZ17IM13R Genewiz (DNA)M13-48REV Genewiz (DNA)BspQI,SapIpUCori5F (DNA)PciINspIoLSM-pUC_ori_R (DNA)

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