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WTSp_2.0_lentiCRISPRv2
  • Sequence Map
    Editing disabled on read-only sequence.
    CATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAAWPRE20406080100120140160
    GCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCCGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTTAGTAGCAGGAAAGGAACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGWPRE180200220240260280300320340
    TCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCGTCGACTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGCAGCTGAAATTCTGGTTACTGAATGTTCCGTCGACATCTAGAATCGGTGAAAAATTTTCTTTTCSacIIWPRE360380400420440460480500520
    GGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTTCTGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGAflII3' LTR (ΔU3)540560580600620640660680700
    TGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCAACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCCCGGGCAAATTTGGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTApaIPmeIPspOMIbGH poly(A) signal3' LTR (ΔU3)720740760780800820840860
    CTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGACCCCbGH poly(A) signal8809009209409609801,0001,0201,040
    CTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGGAGATCCCCCATAGGGGTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCf1 ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGDrdIf1 ori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAATTAAGACACCTTACACACAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTANsiIPsiIf1 oriSV40 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    CTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAANsiISV40 promoterSV40 ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    TTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTAAATAAATACGTCTCCGGCTCCGGCGGAGACGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTTCGAGGGCCCTCGAACATATAGGTAAAAGCCTAGACTAGTCGTGCACAACTGTTAATTAGTAGCCGTATCATATAGCCGTATCATSmaIStuIXmaIAvrIITspMISV40 promoterSV40 oriEM7 promoter1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TAATACGACAAGGTGAGGAACTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCAATTATGCTGTTCCACTCCTTGATTTGGTACCGGTTCAACTGGTCACGGCAAGGCCACGAGTGGCGCGCGCTGCAGCGGCCTCGCCAGCTCAAGACCTGGCTGGCCGAGCCCAAGAGGGCCCTGAAGCACCTCCTGCTGAAGCGGCCACACCAGGCCCTGCTGCACTGGGACAAGTSmaIXmaIMauBISgrAITspMIBleoR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    TCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTAGTCGCGCCAGGTCCTGGTCCACCACGGCCTGTTGTGGGACCGGACCCACACCCACGCGCCGGACCTGCTCGACATGCGGCTCACCAGCCTCCAGCACAGGTGCTTGAAGGCCCTGCGGAGGCCCGGCCGGTACTGGCTCTAGCCGCTCGTCGGCACCCCCGCCCTCAAGCGGGAFseIBleoR2,1202,1402,1602,1802,2002,2202,2402,260
    GCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACACGTGCTACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCGCGCTGGGCCGGCCGTTGACGCACGTGAAGCACCGGCTCCTCGTCCTGACTGTGCACGATGCTCTAAAGCTAAGGTGGCGGCGGAAGATACTTTCCAACCCGAAGCCTTAGCAAAAGGCCCTGCGGCCGACCTACTAGGAGGTCGCGCCCCTAGAGTACGACCTCAAGAAGCGGBleoR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGTGGGGTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACATATGGCAGCTGGAGATCGATCTCGAACCGCATTAGTAPsiIBstZ17ISV40 poly(A) signal2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATCAP binding sitelac promoterM13 revlac operator2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGATACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTPciISapIBspQI2,8202,8402,8602,8802,9002,9202,9402,960
    GCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGADrdIori2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCori3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCori3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAAori3,5203,5403,5603,5803,6003,6203,6403,660
    AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAAmpR3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGAhdIAmpR3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTFspIAmpR4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCPvuIAmpR4,2204,2404,2604,2804,3004,3204,3404,360
    GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCACGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAmpR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAASspIAmpR promoterAmpRAmpR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    AGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGCTGCCTAGCCCTCTAGAGGGCTAGGGGATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCATCSgrDIAmpR promoter4,7404,7604,7804,8004,8204,8404,8604,8804,900
    TGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGTACTTCTTAGACGAATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAACTGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATMluINruISpeICMV enhancer4,9204,9404,9604,9805,0005,0205,0405,060
    GCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCCGGGTATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGCMV enhancer5,0805,1005,1205,1405,1605,1805,2005,2205,240
    AGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTANdeICspCISnaBICMV enhancerCMV promoter5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGCGCCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCGCCMV promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CGTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTGCAAAACGGACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAAflII5' LTR (truncated)5,6205,6405,6605,6805,7005,7205,7405,760
    CAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGTCACACCTTTTAGAGATCGTCACCGCGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATCGCCTCCGATCTTCCTCTCTHIV-1 Ψ5' LTR ...cated)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    GATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTACTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTGTAGTCTTCCGACATNruIHIV-1 Ψ5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACACTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTGGTGGCGTGTSapIBspQI6,1406,1606,1806,2006,2206,2406,2606,2806,300
    GCAAGCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTCGTTCGCCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACAAGGANotIRRE6,3206,3406,3606,3806,4006,4206,4406,460
    TGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTBbvCIRRE6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACATCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTAleIRRE6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAA6,8406,8606,8806,9006,9206,9406,9606,9807,000
    AAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCGGCATTCTTATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGCCGTKflI7,0207,0407,0607,0807,1007,1207,1407,160
    CTGCGTGCGCCAATTCTGCAGACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACGACGCACGCGGTTAAGACGTCTGTTTACCGTCATAAGTAGGTGTTAAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTAATGTTTTTGTTTAATGTTTTTAAGTTTTAAAAGCCCAAATAATGcPPT/CTS7,1807,2007,2207,2407,2607,2807,3007,3207,340
    AGGGACAGCAGAGATCCAGTTTGGTTAATTAAGGTACCGAGGGCCTATTTCCCATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGGTCCCTGTCGTCTCTAGGTCAAACCAATTAATTCCATGGCTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCKpnIPacIAcc65IU6 promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCGNNNNNNNNNNNNNNNNNNNGTTTAAGAGCTATGCTGGAAACAGCATAGCAAGTTTAAATAAGCATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGCTTTGTGGCNNNNNNNNNNNNNNNNNNNCAAATTCTCGATACGACCTTTGTCGTATCGTTCAAATTTATTCNdeIU6 promotermut gRNA scaffold7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTGAATTCGCTAGCTAGGTCTTGAAAGGAGTGGGAATTGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGATCCGGTGCCTAGCGATCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAAACTTAAGCGATCGATCCAGAACTTTCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTAGGCCACGGATCBmtINheIEcoRIEF-1α core promotermut gRNA scaffold7,7207,7407,7607,7807,8007,8207,8407,860
    AGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGACCGGTTCTAGAGCGCTGCCACCATGGACAAGATCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCTGGCCAAGATCTCGCGACGGTGGTACCTGTTCTAfeIAgeIXbaIEF-1α core promoterCas97,8807,9007,9207,9407,9607,9808,0008,0208,040
    AGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACTCATGTCGTAGCCGGACCTGTAGCCGTGGTTGAGACACCCGACCCGGCACTAGTGGCTGCTCATGTTCCACGGGTCGTTCTTTAAGTTCCACGACCCGTTGTGGCTGGCCGTGTCGTAGTTCTTCTTGGACTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTCCGGTGBstXICas98,0608,0808,1008,1208,1408,1608,1808,2008,220
    CCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCGGCCGACTTCTCTTGGCGGTCTTCTTCTATGTGGTCTGCCTTCTTGGCCTAGACGATAGACGTTCTCTAGAAGTCGTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCTGACCTTCTCAGGAAGGACCACCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCGCas98,2408,2608,2808,3008,3208,3408,3608,3808,400
    AACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACATTGTAGCACCTGCTCCACCGGATGGTGCTCTTCATGGGGTGGTAGATGGTGGACTCTTTCTTTGACCACCTGTCGTGGCTGTTCCGGCTGGACGCCGACTAGATAGACCGGGACCGGGTGTACTAGTTCAAGGCCCCGGTGAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCas98,4208,4408,4608,4808,5008,5208,5408,560
    GCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTCGCTGCACCTGTTCGACAAGTAGGTCGACCACGTCTGGATGTTGGTCGACAAGCTCCTTTTGGGGTAGTTGCGGTCGCCGCACCTGCGGTTCCGGTAGGACAGACGGTCTGACTCGTTCTCGTCTGCCGACCTTTTAGACTAGCGGGTCGACGGGCCGCTCTTCTTCTTACCGGABstXICas98,5808,6008,6208,6408,6608,6808,7008,7208,740
    GTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCAAGCCTTTGGACTAACGGGACTCGGACCCGGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGGCTCCTACGGTTTGACGTCGACTCGTTCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGGGTCTAGCCGCTGGTCATGCGGCTGGACAAAGACCGGCGGTTCTTGCas98,7608,7808,8008,8208,8408,8608,8808,9008,920
    CTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCGACAGGCTGCGGTAGGACGACTCGCTGTAGGACTCTCACTTGTGGCTCTAGTGGTTCCGGGGGGACTCGCGGAGATACTAGTTCTCTATGCTGCTCGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCCGTCGTCGACGGACTCTTCATGTTTCTCTAAAAGAAGCTGGCas98,9408,9608,9809,0009,0209,0409,0609,0809,100
    AGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCATCTCGTTCTTGCCGATGCGGCCGATGTAACTGCCGCCTCGGTCGGTCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGACCTTTTCTACCTGCCGTGGCTCCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCCTTCGTCGCCTGGAAGCTGTTGCCGTCGTAGGGGGTCas99,1209,1409,1609,1809,2009,2209,2409,260
    CCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGGTCTAGGTGGACCCTCTCGACGTGCGGTAAGACGCCGCCGTCCTTCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAGCTCTTCTAGGACTGGAAGGCGTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCGTCTAAGCGGACCTACTGGTCTTTCTCGCTCApaIPspOMICas99,2809,3009,3209,3409,3609,3809,4009,4209,440
    GAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATCTTTGGTAGTGGGGGACCTTGAAGCTCCTTCACCACCTGTTCCCGCGAAGGCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTCTTGGACGGGTTGCTCTTCCACGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCACATATTGCTCGACTGGTTTCACTTTACas99,4609,4809,5009,5209,5409,5609,5809,6009,620
    ACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTTGCACTGGCTCCCTTACTCTTTCGGGCGGAAGGACTCGCCGCTCGTCTTTTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTGGCACTTCGTCGACTTTCTCCTGATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAGAGGCCGCACCTTCTAGCCAACas99,6409,6609,6809,7009,7209,7409,7609,7809,800
    CAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACGTTGCGGAGGGACCCGTGTATGGTGCTAGACGACTTTTAATAGTTCCTGTTCCTGAAGGACCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTATAGCACGACTGGGACTGTGACAAACTCCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGGGTGGACAAGCTGCTGPasIEcoRVCas99,8209,8409,8609,8809,9009,9209,9409,960
    AAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTTCACTACTTCGTCGACTTCGCCGCCTCTATGTGGCCGACCCCGTCCGACTCGGCCTTCGACTAGTTGCCGTAGGCCCTGTTCGTCAGGCCGTTCTGTTAGGACCTAAAGGACTTCAGGCTGCCGAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTGTCGGACTGGAAhdIAjuICas99,98010,00010,02010,04010,06010,08010,10010,12010,140
    TTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATTTCTCCTGTAGGTCTTTCGGGTCCACAGGCCGGTCCCGCTATCGGACGTGCTCGTGTAACGGTTAGACCGGCCGTCGGGGCGGTAATTCTTCCCGTAGGACGTCTGTCACTTCCACCACCTGCTCGAGCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCACTAGCTCas910,16010,18010,20010,22010,24010,26010,28010,30010,320
    AATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGTTACCGGTCTCTCTTGGTCTGGTGGGTCTTCCCTGTCTTCTTGTCGGCGCTCTCTTACTTCGCCTAGCTTCTCCCGTAGTTTCTCGACCCGTCGGTCTAGGACTTTCTTGTGGGGCACCTTTTGTGGGTCGACGTCTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCCCas910,34010,36010,38010,40010,42010,44010,46010,48010,500
    GATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGACTATACATGCACCTGGTCCTTGACCTGTAGTTGGCCGACAGGCTGATGCTACACCTGGTATAGCACGGAGTCTCGAAAGACTTCCTGCTGAGGTAGCTGTTGTTCCACGACTGGTCTTCGCTGTTCTTGGCCCCGTTCTCGCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTCas910,52010,54010,56010,58010,60010,62010,64010,660
    TGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCACTTCTTGATGACCGCCGTCGACGACTTGCGGTTCGACTAATGGGTCTCTTTCAAGCTGTTAGACTGGTTCCGGCTCTCTCCGCCGGACTCGCTTGACCTATTCCGGCCGAAGTAGTTCTCTGTCGACCACCTTTGGGCCGTCTAGTGTTTCGTGCACCGTGTCTAGGACCTGAGCspCICas910,68010,70010,72010,74010,76010,78010,80010,82010,840
    CCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGGCCTACTTGTGATTCATGCTGCTCTTACTGTTCGACTAGGCCCTTCACTTTCACTAGTGGGACTTCAGGTTCGACCACAGGCTAAAGGCCTTCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTGATGGTGGTGCGGGTGCTGCGGATGGACTTGCGGCAGCACCCTTGGCas910,86010,88010,90010,92010,94010,96010,98011,00011,020
    GCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCACGGGACTAGTTTTTCATGGGATTCGACCTTTCGCTCAAGCACATGCCGCTGATGTTCCACATGCTGCACGCCTTCTACTAGCGGTTCTCGCTCGTCCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCGTTGTAGTACTTGAAAAAGTTCTGGCTCTAATGGGACCGGTAjuICas911,04011,06011,08011,10011,12011,14011,16011,18011,200
    ACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCTGCCGCTCTAGGCCTTCGCCGGAGACTAGCTCTGTTTGCCGCTTTGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAACGGTGGCACGCCTTTCACGACTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCACGTCTGTCCGCCGAAGTCGTTTCTCAGATAGGACGGCas911,22011,24011,26011,28011,30011,32011,34011,360
    CAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAGTTCTCCTTGTCGCTATTCGACTAGCGGTCTTTCTTCCTGACCCTGGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGGATAAGACACGACCACCACCGGTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACACTTTCTCGACGACCCCTAGTGGTAGTACCTTKflIBsaBICas911,38011,40011,42011,44011,46011,48011,50011,52011,540
    AGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCTCTTCGTCGAAGCTCTTCTTAGGGTAGCTGAAAGACCTTCGGTTCCCGATGTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTCATGAGGGACAAGCTCGACCTTTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGACGTCTTCCCTTTGCTTGACCGGGACGXcmICas911,56011,58011,60011,62011,64011,66011,68011,70011,720
    CCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGAGGTTTATACACTTGAAGGACATGGACCGGTCGGTGATACTCTTCGACTTCCCGAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCTTGTCGTGTTCGTGATGGACCTGCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCACTAGGACCGGCTGCGATTAGACas911,74011,76011,78011,80011,82011,84011,86011,88011,900
    GGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCCCTGTTTCACGACAGGCGGATGTTGTTCGTGGCCCTATTCGGGTAGTCTCTCGTCCGGCTCTTATAGTAGGTGGACAAATGGGACTGGTTAGACCCTCGGGGACGGCGGAAGTTCATGAAACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTTCTCCACGACCTGCGGXcmICas911,92011,94011,96011,98012,00012,02012,04012,060
    ACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAGCGACCTGCCGCCACAAAGAAGGCTGGACAGGCTAAGAAGAAGAAAGATTACAAAGACGATGACGATAAGGGATCCGGCGCAACAAACTTCTCTCTGCTGAAACTGGGACTAGGTGGTCTCGTAGTGGCCGGACATGCTCTGTGCCTAGCTGGACAGAGTCGACCCTCCGCTGTTCGCTGGACGGCGGTGTTTCTTCCGACCTGTCCGATTCTTCTTCTTTCTAATGTTTCTGCTACTGCTATTCCCTAGGCCGCGTTGTTTGAAGAGAGACGACTTTGBamHICas9nucleoplasmin NLSP2AFLAG12,08012,10012,12012,14012,16012,18012,20012,22012,240
    AAGCCGGAGATGTCGAAGAGAATCCTGGACCGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTTTCGGCCTCTACAGCTTCTCTTAGGACCTGGCTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGAPasIBsiWIPflFIRsrIIBstEIITth111IPuroRP2A12,26012,28012,30012,32012,34012,36012,38012,40012,420
    GCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTSacIIPuroR12,44012,46012,48012,50012,52012,54012,56012,58012,600
    CAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGStuIPuroR12,62012,64012,66012,68012,70012,72012,74012,760
    GCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTAAGTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAATTCAGCTGTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAMluIPuroRWPRE12,78012,80012,82012,84012,86012,88012,90012,92012,940
    TGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAWPRE12,96012,98013,00013,020
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    13022 bp9000100001100012000100020003000Cas9oriSV40 oriWPRE

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    13022