Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
Advanced Settings
pGEX-KG-D4H*-mCherry
  • Sequence Map
Editing disabled on read-only sequence.
AATTACGTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTTTAATGCAATAGCTGACGTGCCACGTGGTTACGAAGACCGCAGTCCGTCGGTAGCCTTCGACACCATACCGACACGTCCAGCATTTAGTGACGTATTAAGCACAGCGAGTTCCGCGTGAGGGCAAGACCTATTACAAAAAACGCGGCTGTAGTATTGCCAAGACCGTTTATAAGASspIBfuAIBspMI20406080100120140160
GAAATGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACGCAGGAAACAGTATTCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGACTTTACTCGACAACTGTTAATTAGTAGCCGAGCATATTACACACCTTAACACTCGCCTATTGTTAAAGTGCGTCCTTTGTCATAAGTACAGGGGATATGATCCAATAACCTTTTAATTCCCGGAACACGTTGGGTGAGCTGAAGAAAACCTTATAGAACTTCTTTTTATACTTCTSapIBspQIEcoNIEcoO109IL1T2I3N4H5R6L7V8*9C10V11E12L13*14A15D16N17N18F19T20Q21E22T23V24F25187-261MS2PI4LG6YW8KI10KG12LV14QP16TR18LL20LE22YL24EE26KY28EE30262-2211GSTtacLacO180200220240260280300320340
GCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTAAACATACTCGCGCTACTTCCACTATTTACCGCTTTGTTTTTCAAACTTAACCCAAACCTCAAAGGGTTAGAAGGAATAATATAACTACCACTACAATTTAATTGTGTCAGATACCGGTAGTATGCAATATATCGACTGTTCGTGTTGTACAACCCACCAACAGGTTTTCTCMscIPciIHL32YE34RD36EG38DK40WR42NK44KF46EL48GL50EF52PN54LP56YY58ID60GD62VK64LT66QS68MA70II72RY74IA76DK78HN80ML82GG84CP86KE88262-2211GST360380400420440460480500520
CGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCGCACGTCTCTAAAGTTACGAACTTCCTCGCCAAAACCTATAATCTATGCCACAAAGCTCTTAACGTATATCATTTCTGAAACTTTGAGAGTTTCAACTAAAAGAATCGTTCGATGGACTTTACGACTTTTACAAGCTTCTAGCAAATACAGTATTTTGTATAAATTTACCACTAGBclISwaIXmnIBstBIRA90EI92SM94LE96GA98VL100DI102RY104GV106SR108IA110YS112KD114FE116TL118KV120DF122LS124KL126PE128ML130KM132FE134DR136LC138HK140TY142LN144GD146262-2211GST540560580600620640660680700
ATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGTACATTGGGTAGGACTGAAGTACAACATACTGCGAGAACTACAACAAAATATGTACCTGGGTTACACGGACCTACGCAAGGGTTTTAATCAAACAAAATTTTTTGCATAACTTCGATAGGGTGTTTAACTATTCATGAACTTTAGGTCGTTCATATATCGTACCGGAAACGTCCCScaIHV148TH150PD152FM154LY156DA158LD160VV162LY164MD166PM168CL170DA172FP174KL176VC178FK180KR182IE184AI186PQ188ID190KY192LK194SS196KY198IA200WP202LQ204G262-2211GST720740760780800820840860
CTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGGAATTTCCGGTGGTGGTGGTGGAATTCTAGACTCCATGGGTCGACTCGAGATGAAGGGAAAAATAAACTTAGATCATAGTGGAGCCTATGTTGCACAGTTTGAAGTAGACCGTTCGGTGCAAACCACCACCGCTGGTAGGAGGTTTTAGCCTAGACCAAGGCGCACCTAGGGGCCCTTAAAGGCCACCACCACCACCTTAAGATCTGAGGTACCCAGCTGAGCTCTACTTCCCTTTTTATTTGAATCTAGTATCACCTCGGATACAACGTGTCAAACTTCATAccINcoISalISmaITliIXbaIXhoIXmaIBamHIEcoRITspMIPaeR7IW206QA208TF210GG212GD214HP216PK218SD220LV222PR224GS226PG228IS230GG232GG234GI236LD238SM240GR242LE244MK246GK248IN250LD252HS254GA256YV258AQ260FE262V262-2211D4H-YDAGSTThrombin site8809009209409609801,0001,0201,040
GCCTGGGATGAAGTTTCAGCCGACAAAGAAGGAAATGAAGTTTTAACTCATAAAACATGGGATGGAAATTATCAATCTAAAACAGCTCACTATTCAACAGTAATACCTCTTGAAGCTAATGCAAGAAATATAAGAATAAAAGCAAGAGAGTGTACAGGCCTTTGGTGGGAATGGTCGGACCCTACTTCAAAGTCGGCTGTTTCTTCCTTTACTTCAAAATTGAGTATTTTGTACCCTACCTTTAATAGTTAGATTTTGTCGAGTGATAAGTTGTCATTATGGAGAACTTCGATTACGTTCTTTATATTCTTATTTTCGTTCTCTCACATGTCCGGAAACCACCCTTACCABsrGIA264WD266EV268SA270DK272EG274NE276VL278TH280KT282WD284GN286YQ288SK290TA292HY294ST296VI298PL300EA302NA304RN306IR308IK310AR312EC314TG316LW318WE320W262-2211D4H-YDA1,0601,0801,1001,1201,1401,1601,1801,2001,220
GGAGAGATGTTATAAGTGAATATGATGTTCCATTAACAAATAATATAAATGTTTCAATATGGGGAACAACTTTATACCCTGGATCTAGTATTACTTACAATAGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCAATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCCTCTCTACAATATTCACTTATACTACAAGGTAATTGTTTATTATATTTACAAAGTTATACCCCTTGTTGAAATATGGGACCTAGATCATAATGAATGTTATCTTAAGACGTCAGCTGCCATGGCGCCCGGGCCCTAGGTTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAAccIApaIKpnIMscIPsiISalISmaIXmaIBamHIEcoRISacIITspMIAcc65IPspOMIW322RD324VI326SE328YD330VP332LT334NN336IN338VS340IW342GT344TL346YP348GS350SI352TY354NR356IL358QS360TV362PR364AR366DP368MV370SK372GE374ED376NM378AI380262-2211D4H-YDAmCherry1,2401,2601,2801,3001,3201,3401,3601,3801,400
CATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGAhdIIK382EF384MR386FK388VH390ME392GS394VN396GH398EF400EI402EG404EG406EG408RP410YE412GT414QT416AK418LK420VT422KG424GP426LP428FA430WD432IL434SP436QF438262-2211mCherry1,4201,4401,4601,4801,5001,5201,5401,560
ATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAGGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGAloISbfIBsaAIMY440GS442KA444YV446KH448PA450DI452PD454YL456KL458SF460PE462GF464KW466ER468VM470NF472ED474GG476VV478TV480TQ482DS484SL486QD488GE490FI492YK494VK496262-2211mCherry1,5801,6001,6201,6401,6601,6801,7001,7201,740
TGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTBplIBplINcoIAlwNIBbvCIPflMIBpu10ILR498GT500NF502PS504DG506PV508MQ510KK512TM514GW516EA518SS520ER522MY524PE526DG528AL530KG532EI534KQ536RL538KL540KD542GG544HY546DA548EV550KT552TY554K262-2211mCherry1,7601,7801,8001,8201,8401,8601,8801,9001,920
GGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGGCGGCCGCGACTCTAGATCATAATCAGCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCCGCCGGCGCTGAGATCTAGTATTAGTCAleIEagINotIXbaIBsaBIBsrGISgrAIA556KK558PV560QL562PG564AY566NV568NI570KL572DI574TS576HN578ED580YT582IV584EQ586YE588RA590EG592RH594ST596GG598MD600EL602YK604AA606AT608LD610HN612Q262-2211mCherry1,9401,9601,9802,0002,0202,0402,0602,0802,100
CCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAGCTTAATTCATCGTGACTGACTGACGATCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCGGTATGGTGTAAACATCTCCAAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGTATTTTACTTACGTTAACAACAACAATCGAATTAAGTAGCACTGACTGACTGCTAGACGGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGBsmIMfeIPfoIBsmBIEsp3IP614YH616IC618RG620FT622CF624KK626PP628TP630PP632EP634ET636*N638EC640NC642CC644*L646NS648S*650262-22112,1202,1402,1602,1802,2002,2202,2402,260
CCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGTCGGTACTGGGTCAGTGCATCGCTATCGCCTCACATATTAAGAACTTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCBsaAIPflFITth111IEcoO109I2,2802,3002,3202,3402,3602,3802,4002,4202,440
TTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAAATTACAGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTSspIZraIAatIIAmp prom2,4602,4802,5002,5202,5402,5602,5802,6002,620
ACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAATGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTT2,6402,6602,6802,7002,7202,7402,7602,7802,800
CGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCACAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATAScaIXmnIAmpR2,8202,8402,8602,8802,9002,9202,9402,960
GCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACCGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGPvuIAmpR2,9803,0003,0203,0403,0603,0803,1003,1203,140
GATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCTACGGACGTCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCBglIFspIAmpR3,1603,1803,2003,2203,2403,2603,2803,3003,320
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATAGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATAhdIBsaIAmpR3,3403,3603,3803,4003,4203,4403,4603,4803,500
TACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACColE1 origin3,5203,5403,5603,5803,6003,6203,6403,660
CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTColE1 origin3,6803,7003,7203,7403,7603,7803,8003,8203,840
TACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGAlwNIColE1 origin3,8603,8803,9003,9203,9403,9603,9804,0004,020
TACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAAColE1 origin4,0404,0604,0804,1004,1204,1404,1604,1804,200
GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTPciIColE1 origin4,2204,2404,2604,2804,3004,3204,3404,360
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAAATTCCGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATTTAAGGCTGTGGTAGCTTACCACGTTTTGGAAAGCGCCATACCGTACTATCGCGGGCCTTCTCTCAGTTAAGTCCCACCSapIBspQIPflMI4,3804,4004,4204,4404,4604,4804,5004,5204,540
TGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCACTTACACTTTGGTCATTGCAATATGCTACAGCGTCTCATACGGCCACAGAGAATAGTCTGGCAAAGGGCGCACCACTTGGTCCGGTCGGTGCAAAGACGCTTTTGCGCCCTTTTTCACCTTCGCCGCTACCGCCTCGACTTAATGTAAGGGTTGGCGCACCGTGTTGTTGACCGBstAPI4,5604,5804,6004,6204,6404,6604,6804,7004,720
GGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCCCCGTTTGTCAGCAACGACTAACCGCAACGGTGGAGGTCAGACCGGGACGTGCGCGGCAGCGTTTAACAGCGCCGCTAATTTAGAGCGCGGCTAGTTGACCCACGGTCGCACCACCACAGCTACCATCTTGCTTCGCCGCAGCTTCGGACATTTCGCCGCCACGTGTTAGAAGAG4,7404,7604,7804,8004,8204,8404,8604,8804,900
GCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCCGCGTTGCGCAGTCACCCGACTAGTAATTGATAGGCGACCTACTGGTCCTACGGTAACGACACCTTCGACGGACGTGATTACAAGGCCGCAATAAAGAACTACAGAGACTGGTCTGTGGGTAGTTGTCATAATAAAAGAGGGTACTTCTGCCATGCGCTGACCCGCACCTCGTAGBclIMluI4,9204,9404,9604,9805,0005,0205,0405,060
TGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATACCAGCGTAACCCAGTGGTCGTTTAGCGCGACAATCGCCCGGGTAATTCAAGACAGAGCCGCGCAGACGCAGACCGACCGACCGTATTTATAGAGTGAGCGTTAGTTTAAGTCGGCTATCGCCTTGCCCTTCCGCTGACCTCACGGTACAGGCCAAAAGTTGTTTGGTACGTTTAApaIPspOMI5,0805,1005,1205,1405,1605,1805,2005,2205,240
GCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACGACTTACTCCCGTAGCAAGGGTGACGCTACGACCAACGGTTGCTAGTCTACCGCGACCCGCGTTACGCGCGGTAATGGCTCAGGCCCGACGCGCAACCACGCCTATAGAGCCATCACCCTATGCTGCTATGGCTTCTGTCGAGTACAATATAGGGCGGCAGTTGGTGGTAGTTTEcoRVBssHII5,2605,2805,3005,3205,3405,3605,3805,4005,420
CAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCGTCCTAAAAGCGGACGACCCCGTTTGGTCGCACCTGGCGAACGACGTTGAGAGAGTCCCGGTCCGCCACTTCCCGTTAGTCGACAACGGGCAGAGTGACCACTTTTCTTTTTGGTGGGACCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGBsmBIEsp3I5,4405,4605,4805,5005,5205,5405,5605,5805,600
TGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAATGClacLacOM13-rev5,6205,6405,6605,6805,7005,7205,7405,760
GATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTCTAAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGAAACGGACCABglIFspIPvuILacZ alphaM13-fwd5,7805,8005,8205,8405,8605,8805,9005,9205,940
TTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTAACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGAAGGCCGTGGTCTTCGCCACGGCCTTTCGACCGACCTCACGCTAGAAGGACTCCGGCTATGACAGCAGCAGGGGAGTTTGACCGTCTACGTGCCAATGCTACGCGGGTAGATGTGGTTGCATTGGATAGGGTAATGCCAGTTAGGCGGCAAACAAGGGTGCCTCTTAGGCTGCCCBaeIBsu36I5,9605,9806,0006,0206,0406,0606,0806,1006,120
TTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTGGAACAATGAGCGAGTGTAAATTACAACTACTTTCGACCGATGTCCTTCCGGTCTGCGCTTAATAAAAACTACCGCAACC6,1406,1606,1806,200
  • Plasmid
Editing disabled on read-only sequence.
6203 bp45005000550050010001500mCherryA463W187-261262-2211

Welcome to Benchling!

Sign in to view data.

New digest

Saved digests

Enzyme AatII
Enzyme lists
Cut sites visible on maps
Find enzyme
Name Cuts
Selected Color
Show enzymes that cut

Primers

History

Lineage

 Show Full History
Change User Timestamp

Sequence Alignments

Saved Alignments

Sequence Info

Tags

Name
Parent Version
Folder
Topology
Start Index
Locked
Created
--
Length
--
Export Data
Split Workspace
Bases
6203