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XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export (SV40-PuroR-BFP)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatgg20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIAmp180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaFspIAmp360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattAmp540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaScaIAmp720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataAmp8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaCspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggAloIPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcatt1,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagFspI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataaBII(R)5'TR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataatttttttt31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgccccore insul2,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctataMfeISfiISrfIcore insulPCRhomologho...g2,2802,3002,3202,3402,3602,3802,4002,4202,440
    acgcgtGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTtgcgcaCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCAAgeIMfeIMluIEF-1α promoterprime...F-1a2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGTATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCEF-1α promoter2,6402,6602,6802,7002,7202,7402,7602,7802,800
    GTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACAflIIEF-1α promoter2,8202,8402,8602,8802,9002,9202,9402,960
    CTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTBglIIEF-1α promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    AGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCFseISacIEco53kIEF-1α promoter3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCBbvCIEF-1α promoterfrag13,3403,3603,3803,4003,4203,4403,4603,4803,500
    AGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAATCTAGAAGCCACCATGGCGGCCCCTGCTCTGTCCTGGCGTTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGTACCGCCGGGGACGAGACAGGACCGCAXbaIMA2AP4AL6SW8R3649-5781EF-1α promoterfrag1RF from EF-1aIL-2Rbeta ...l Peptidehomolog3,5203,5403,5603,5803,6003,6203,6403,660
    CTGCCCCTCCTCATCCTCCTCCTGCCCCTGGCTACCTCTTGGGCATCTGCAgactacaaggatgacgatgataagGGTACCGCGGTGAATGGCACTTCCCAGTTCACATGCTTCTACAATTCGAGAGCCAACATCTCCTGTGTCTGGAGCCAAGATGGGGCTCTGCAGGACACTTGACGGGGAGGAGTAGGAGGAGGACGGGGACCGATGGAGAACCCGTAGACGTctgatgttcctactgctactattcCCATGGCGCCACTTACCGTGAAGGGTCAAGTGTACGAAGATGTTAAGCTCTCGGTTGTAGAGGACACAGACCTCGGTTCTACCCCGAGACGTCCTGTGAABaeIL10PL12LI14LL16LP18LA20TS22WA24SA26DY28KD30DD32DK34GT36AV38NG40TS42QF44TC46FY48NS50RA52NI54SC56VW58SQ60DG62AL64QD66T3649-5781hIL-2Rbeta ectodomainIL-2Rbeta Signal PeptideFLAGhomologhomolog3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CCTGCCAAGTCCATGCCTGGCCGGACAGACGGCGGTGGAACCAAACCTGTGAGCTGCTCCCCGTGAGTCAAGCATCCTGGGCCTGCAACCTGATCCTCGGAGCCCCAGATTCTCAGAAACTGACCACAGTTGACATCGTCACCCTGAGGGTGCTGTGCCGTGAGGGGGTGCGATGGGACGGTTCAGGTACGGACCGGCCTGTCTGCCGCCACCTTGGTTTGGACACTCGACGAGGGGCACTCAGTTCGTAGGACCCGGACGTTGGACTAGGAGCCTCGGGGTCTAAGAGTCTTTGACTGGTGTCAACTGTAGCAGTGGGACTCCCACGACACGGCACTCCCCCACGCTACBsu36IS68CQ70VH72AW74PD76RR78RW80NQ82TC84EL86LP88VS90QA92SW94AC96NL98IL100GA102PD104SQ106KL108TT110VD112IV114TL116RV118LC120RE122GV124RW1263649-5781hIL-2Rbeta ectodomain3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GAGGGTGATGGCCATCCAGGACTTCAAGCCCTTTGAGAACCTTCGCCTGATGGCCCCCATCTCCCTCCAAGTTGTCCACGTGGAGACCCACAGATGCAACATAAGCTGGGAAATCTCCCAAGCCTCCCACTACTTTGAAAGACACCTGGAGTTCGAGGCCCGGACGCTGTCCCCACTCCCACTACCGGTAGGTCCTGAAGTTCGGGAAACTCTTGGAAGCGGACTACCGGGGGTAGAGGGAGGTTCAACAGGTGCACCTCTGGGTGTCTACGTTGTATTCGACCCTTTAGAGGGTTCGGAGGGTGATGAAACTTTCTGTGGACCTCAAGCTCCGGGCCTGCGACAGGGGTRV128MA130IQ132DF134KP136FE138NL140RL142MA144PI146SL148QV150VH152VE154TH156RC158NI160SW162EI164SQ166AS168HY170FE172RH174LE176FE178AR180TL182SP1843649-5781hIL-2Rbeta ectodomain4,0404,0604,0804,1004,1204,1404,1604,1804,200
    GGCCACACCTGGGAGGAGGCCCCCCTGCTGACTCTCAAGCAGAAGCAGGAATGGATCTGCCTGGAGACGCTCACCCCAGACACCCAGTATGAGTTTCAGGTGCGGGTCAAGCCTCTGCAAGGCGAGTTCACGACCTGGAGCCCCTGGAGCCAGCCCCTGGCCTTCAGGACAAAGCCCGGTGTGGACCCTCCTCCGGGGGGACGACTGAGAGTTCGTCTTCGTCCTTACCTAGACGGACCTCTGCGAGTGGGGTCTGTGGGTCATACTCAAAGTCCACGCCCAGTTCGGAGACGTTCCGCTCAAGTGCTGGACCTCGGGGACCTCGGTCGGGGACCGGAAGTCCTGTTTCGXcmIGH186TW188EE190AP192LL194TL196KQ198KQ200EW202IC204LE206TL208TP210DT212QY214EF216QV218RV220KP222LQ224GE226FT228TW230SP232WS234QP236LA238FR240TK2423649-5781hIL-2Rbeta ectodomain4,2204,2404,2604,2804,3004,3204,3404,360
    CTGCAGCCCTTGGGAAGGACACCATTCCGTGGCTCGGCCACCTCCTCGTGGGCCTCAGCGGGGCTTTTGGCTTCATCATCTTAGTGTACTTGCTCATCGGTACCgcggccgccaagagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgTTGACGTCGGGAACCCTTCCTGTGGTAAGGCACCGAGCCGGTGGAGGAGCACCCGGAGTCGCCCCGAAAACCGAAGTAGTAGAATCACATGAACGAGTAGCCATGGcgccggcggttctcgtcctagtggtcgctcccgctcatgtagggggacctggtctagctgtagttgcacAABbvCIG1T2A1A2A3PA244AL246GK248DT250IP252WL254GH256LL258VG260LS262GA264FG266FI268IL270VY272LL274IG276TA278AA280KS282RI284TS286EG288EY290IP292LD294QI296DI298NV300L3649-5781hIL-2Rbeta TransmembraneTraffic signalhIL-2Rbe...odomainPCRhomolog4,3804,4004,4204,4404,4604,4804,5004,5204,540
    GCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGCGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACH302LD304QT306PS308RQ310PI312PS314EG316LQ318LH320LP322QV324LA326DA328VS330RL332VL334GK336FG338DL340TD342NF344SS346PH348AR350RK352VL354AG356VV358M3649-5781hADAR2 cat domain4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAATGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTT360TG362TD364VK366DA368KV370IS372VS374TG376TK378CI380NG382EY384MS386DR388GL390AL392ND394CH396AE398II400SR402RS404LL406RF408LY410TQ412LE414LY416L3649-5781hADAR2 cat domain4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCBsrGIN418NK420DD422QK424RS426IF428QK430SE432RG434GF436RL438KE440NV442QF444HL446YI448ST450SP452CG454DA456RI458FS460PH462EP464IL466EE468PA470DR472HP474NR4763649-5781hADAR2 cat domain4,9204,9404,9604,9805,0005,0205,0405,060
    TAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACKA478RG480QL482RT484KI486ES488GQ490GT492IP494VR496SN498AS500IQ502AW504DG506VL508QG510ER512LL514TM516SC518SD520KI522AR524WN526VV528GI530QG532SL5343649-5781hADAR2 cat domainhomologousDim...ce5,0805,1005,1205,1405,1605,1805,2005,2205,240
    CTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGBlpILS536IF538VE540PI542YF544SS546II548LG550SL552YH554GD556HL558SR560AM562YQ564RI566SN568IE570DL572PP574LY576TL578NK580PL582LS584GI586SN588AE590AR5923649-5781hADAR2 cat domainhomolog5,2605,2805,3005,3205,3405,3605,3805,4005,420
    AGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCMluIQP594GK596AP598NF600SV602NW604TV606GD608SA610IE612VI614NA616TT618GK620DE622LG624RA626SR628LC630KH632AL634YC636RW638MR640VH642GK644VP646SH648LL650R3649-5781hADAR2 cat domain5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCttctgctacgagaacgagGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGaagacgatgctcttgctcS652KI654TK656PN658VY660HE662SK664LA666AK668EY670QA672AK674AR676LF678TA680FI682KA684GL686GA688WV690EK692PT694EQ696DQ698FS700LT702PF704CY706EN708E3649-5781hADAR2 cat domainER export5,6205,6405,6605,6805,7005,7205,7405,760
    gtgtgaTAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCcacactATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGAvrIIV710*EMCV IRES Sitehomolog5,7805,8005,8205,8405,8605,8805,9005,9205,940
    CCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCPaqCIHindIIIEMCV IRES Site5,9605,9806,0006,0206,0406,0606,0806,1006,120
    CGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTEMCV IRES Site6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGTTGAAGCCATCATTACCATTCACATCCCTCTTATTCCTGCAGCTGCCCCTGCTGGGAGTGGGGGCGGCCGTTTACCCATACGATGTTCCTGACTATGCGGGTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACAACTTCGGTAGTAATGGTAAGTGTAGGGAGAATAAGGACGTCGACGGGGACGACCCTCACCCCCGCCGGCAAATGGGTATGCTACAAGGACTGATACGCCCBmgBIML2KP4SL6PF8TS10LL12FL14QL16PL18LG20VG22AA24VY26PY28DV30PD32YA34G6372-7334EMCV IRES SitehIL-2Rgamma Signal Peptide3xHAhomologhomolog6,3206,3406,3606,3806,4006,4206,4406,460
    CTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTCCGGCCGCCGGTACCCTGAACACGACAATTCTGACGCCCAATGGGAATGAAGACACCACAGCTGATTTCTTCCTGACCACTATGCCCACTGACTCCCTCAGTGTTTCCACTCTGGATAGGGATACTGCAGGGCCTGATACGTCCTAGGATAGGTATACTGCAAGGTCTAATGCGAGGCCGGCGGCCATGGGACTTGTGCTGTTAAGACTGCGGGTTACCCTTACTTCTGTGGTGTCGACTAAAGAAGGACTGGTGATACGGGTGACTGAGGGAGTCACAAAGGTGAGACNdeIY36PY38DV40PD42YA44GS46YP48YD50VP52DY54AP56AA58GT60LN62TT64IL66TP68NG70NE72DT74TA76DF78FL80TT82MP84TD86SL88SV90ST92L6372-7334hIL-2Rgamma Ectodomain3xHAhomoolog6,4806,5006,5206,5406,5606,5806,6006,6206,640
    CCCCTCCCAGAGGTTCAGTGTTTTGTGTTCAATGTCGAGTACATGAATTGCACTTGGAACAGCAGCTCTGAGCCCCAGCCTACCAACCTCACTCTGCATTATTGGTACAAGAACTCGGATAATGATAAAGTCCAGAAGTGCAGCCACTATCTATTCTCTGAAGAAATCACTTCTGGGGGAGGGTCTCCAAGTCACAAAACACAAGTTACAGCTCATGTACTTAACGTGAACCTTGTCGTCGAGACTCGGGGTCGGATGGTTGGAGTGAGACGTAATAACCATGTTCTTGAGCCTATTACTATTTCAGGTCTTCACGTCGGTGATAGATAAGAGACTTCTTTAGTGAAGACP94LP96EV98QC100FV102FN104VE106YM108NC110TW112NS114SS116EP118QP120TN122LT124LH126YW128YK130NS132DN134DK136VQ138KC140SH142YL144FS146EE148IT150S6372-7334hIL-2Rgamma Ectodomain6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GCTGTCAGTTGCAAAAAAAGGAGATCCACCTCTACCAAACATTTGTTGTTCAGCTCCAGGACCCACGGGAACCCAGGAGACAGGCCACACAGATGCTAAAACTGCAGAATCTGGTGATCCCCTGGGCTCCAGAGAACCTAACACTTCACAAACTGAGTGAATCCCAGCTAGAACTCGACAGTCAACGTTTTTTTCCTCTAGGTGGAGATGGTTTGTAAACAACAAGTCGAGGTCCTGGGTGCCCTTGGGTCCTCTGTCCGGTGTGTCTACGATTTTGACGTCTTAGACCACTAGGGGACCCGAGGTCTCTTGGATTGTGAAGTGTTTGACTCACTTAGGGTCGATCTTGAG152CQ154LQ156KK158EI160HL162YQ164TF166VV168QL170QD172PR174EP176RR178QA180TQ182ML184KL186QN188LV190IP192WA194PE196NL198TL200HK202LS204ES206QL208EL2106372-7334hIL-2Rgamma Ectodomain6,8406,8606,8806,9006,9206,9406,9606,9807,000
    GAACTGGAACAACAGATTCTTGAACCACTGTTTGGAGCACTTGGTGCAGTACCGGACTGACTGGGACCACAGCTGGACTGAACAATCAGTGGATTATAGACATAAGTTCTCCTTGCCTAGTGTGGATGGGCAGAAACGCTACACGTTTCGTGTTCGGAGCCGCTTTAACCCACTCCTTGACCTTGTTGTCTAAGAACTTGGTGACAAACCTCGTGAACCACGTCATGGCCTGACTGACCCTGGTGTCGACCTGACTTGTTAGTCACCTAATATCTGTATTCAAGAGGAACGGATCACACCTACCCGTCTTTGCGATGTGCAAAGCACAAGCCTCGGCGAAATTGGGTGAGAjuINW212NN214RF216LN218HC220LE222HL224VQ226YR228TD230WD232HS234WT236EQ238SV240DY242RH244KF246SL248PS250VD252GQ254KR256YT258FR260VR262SR264FN266PL2686372-7334hIL-2Rgamma Ectodomain7,0207,0407,0607,0807,1007,1207,1407,160
    TGTGGAAGTGCTCAGCATTGGAGTGAATGGAGCCACCCAATCCACTGGGGGAGCAATACTTCAAAAGAGAATCCTTTCCTGTTTGCATTGGAAGCCGTGGTTATCTCTGTTGGCTCCATGGGATTGATTATCAGCCTTCTCTGTGTGTATTTCTGGCTGGGTACCgcggccgccaACACCTTCACGAGTCGTAACCTCACTTACCTCGGTGGGTTAGGTGACCCCCTCGTTATGAAGTTTTCTCTTAGGAAAGGACAAACGTAACCTTCGGCACCAATAGAGACAACCGAGGTACCCTAACTAATAGTCGGAAGAGACACACATAAAGACCGACCCATGGcgccggcggtBlpICG270SA272QH274WS276EW278SH280PI282HW284GS286NT288SK290EN292PF294LF296AL298EA300VV302IS304VG306SM308GL310II312SL314LC316VY318FW320L6372-7334A1A2A3G1T2hIL-2Rgamma EctodomainhIL-2Rgamma Transmembrane7,1807,2007,2207,2407,2607,2807,3007,3207,340
    agagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgATGGCGAGCAACTTTACGCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCAAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGtctcgtcctagtggtcgctcccgctcatgtagggggacctggtctagctgtagttgcacTACCGCTCGTTGAAATGCGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGTTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCKS2RI4TS6EG8EY10IP12LD14QI16DI18NV20MA22SN24FT26QF28VL30VD32NG34GT36GD38VT40VA42PS44NF46AN48GI50AE52WI54SS56NS58R7350-8159MCPTraffic signalPCR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    GAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAAGTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAACTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTXcmIS60QA62YK64VT66CS68VR70QS72SA74QN76RK78YT80IK82VE84VP86KG88AW90RS92YL94NM96EL98TI100PI102FA104TN106SD108CE110LI112VK114AM116Q7350-8159MCP7,5407,5607,5807,6007,6207,6407,6607,6807,700
    GGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGGCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACCGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCAgeIAsiSIG118LL120KD122GN124PI126PS128AI130AA132NS134GI136YG138GG140TG142GG144SM146AS148NF150TQ152FV154LV156DN158GG160TG162DV164TV166AP168SN170FA172NG174I7350-8159MCPMCP7,7207,7407,7607,7807,8007,8207,8407,860
    CCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACBstZ17IA176EW178IS180SN182SR184SQ186AY188KV190TC192SV194RQ196SS198AQ200NR202KY204TI206KV208EV210PK212GA214WR216SY218LN220ME222LT224IP226IF228AT230NS232DC2347350-8159MCPhomolog7,8807,9007,9207,9407,9607,9808,0008,0208,040
    CGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGAATTTACttctgctacgagaacgaggtgtgatgatagccgGCTAGCtcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgcGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGCCTTAAATGaagacgatgctcttgctccacactactatcggcCGATCGagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcgBmtINheIPspXIEL236IV238KA240MQ242GL244LK246DG248NP250IP252SA254IA256AN258SG260IY262FC264YE266NE268V*2707350-8159MCPc-myc epitopeER exporthomolog8,0608,0808,1008,1208,1408,1608,1808,2008,220
    cgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagacPmeISalIBGH polyadenylation sequencePolyhi...e tag8,2408,2608,2808,3008,3208,3408,3608,3808,400
    agtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatBGH polyadenylation sequencepSV405, 299 Fhomolog8,4208,4408,4608,4808,5008,5208,5408,560
    tgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccpSV408,5808,6008,6208,6408,6608,6808,7008,7208,740
    ctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgATGACCGAGTACAAGCCCACGGTGgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacTACTGGCTCATGTTCGGGTGCCACAvrIIMT2EY4KP6TV8puroRpSV40PuroRho...g8,7608,7808,8008,8208,8408,8608,8808,9008,920
    CGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCRL10AT12RD14DV16PR18AV20RT22LA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL52FL54TR56VG58LD60IG62KV64WV66puroRPuroRPR_S030PR_S0298,9408,9608,9809,0009,0209,0409,0609,0809,100
    CGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGAAD68DG70AA72VA74VW76TT78PE80SV82EA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME110GL112LA114PH116RP118KE120PA122WF124LpuroRPuroR9,1209,1409,1609,1809,2009,2209,2409,260
    GGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGCCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCA126TV128GV130SP132DH134QG136KG138LG140SA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL168PF170YE172RL174GF176TV178TA180DV182EpuroRPuroR9,2809,3009,3209,3409,3609,3809,4009,4209,440
    GTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtSapIBmgBIBspQIBsrGIPaqCIV184PE186GP188RT190WC192MT194RK196PG198AA200TN202FS204LL206KQ208AG210DV212EE214NP216GP218GS220MV222SK224GE226EL228IK230EN232MH234MK236LY238ME240GpuroRmtagBFP2P2APuroRhomologous9,4609,4809,5009,5209,5409,5609,5809,6009,620
    ccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaT242VD244NH246HF248KC250TS252EG254EG256KP258YE260GT262QT264MR266IK268VV270EG272GP274LP276FA278FD280IL282AT284SF286LY288GS290KT292FI294NH296TQ298GI300puroRmtagBFP29,6409,6609,6809,7009,7209,7409,7609,7809,800
    ccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcAloIBsu36IPD302FF304KQ306SF308PE310GF312TW314ER316VT318TY320ED322GG324VL326TA328TQ330DT332SL334QD336GC338LI340YN342VK344IR346GV348NF350TS352NG354PV356MQ358puroRmtagBFP29,8209,8409,8609,8809,9009,9209,9409,960
    aagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagaKK360TL362GW364EA366FT368ET370LY372PA374DG376GL378EG380RN382DM384AL386KL388VG390GS392HL394IA396NA398KT400TY402RS404KK406PA408KN410LK412MP414GV416puroRmtagBFP29,98010,00010,02010,04010,06010,08010,10010,12010,140
    actatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAgtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTcagctgttagttggagacctaatgttttaaacactttctaactgaccataPacISalIYY418VD420YR422LE424RI426KE428AN430NE432TY434VE436QH438EV440AV442AR444YC446DL448PS450KL452GH454KL456N*458puroRmtagBFP2WPREhomolog10,16010,18010,20010,22010,24010,26010,28010,30010,320
    tcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggttHpaIPsiISV40 pASV40 pA(1)WPREhomolog10,34010,36010,38010,40010,42010,44010,46010,48010,500
    actcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcagggttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtcccaSV40 pASV40 pA(1)10,52010,54010,56010,58010,60010,62010,64010,660
    ttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatcccgtgcaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggcacgcore insul(1) Geneious name: core insul(1)10,68010,70010,72010,74010,76010,78010,80010,82010,840
    caccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctgatatctataacaagaaaagtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggactatagatattgttcttttSrfIEcoRVcore insul(1) Geneious name: core insul(1)10,86010,88010,90010,92010,94010,96010,98011,00011,020
    tatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaEco53kI11,04011,06011,08011,10011,12011,14011,16011,18011,200
    ccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggtSacI3'TR11,22011,24011,26011,28011,30011,32011,34011,360
    agctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttg11,38011,40011,42011,44011,46011,48011,50011,52011,540
    tgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggaacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcct11,56011,58011,60011,62011,64011,66011,68011,70011,720
    cgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattapLac(F)11,74011,76011,78011,80011,82011,84011,86011,88011,900
    gagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgctcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgcSapIBspQI11,92011,94011,96011,98012,00012,02012,04012,060
    gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagct12,08012,10012,12012,14012,16012,18012,20012,22012,240
    cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagt12,26012,28012,30012,32012,34012,36012,38012,40012,420
    gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgcc12,44012,46012,48012,50012,52012,54012,56012,58012,600
    tgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgc12,62012,64012,66012,68012,70012,72012,74012,760
    cgcagaaaaaaaggatctcaagcgtctttttttcctagagtt12,780
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12796 bp9000100001100012000100020003000EF-1α promoterprimer from EF-1aSV40 pASV40 pA(1)Gibson overlapMluI-PGK-GFAmppuroREWo102.30 (DNA)XZp336.1 (DNA)XZp075.1 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),AhdI,FspIScaICspCI,PsiI,AloIFrtF (DNA)FlpInR (DNA),FspIBII(R),M13-40FOR Genewiz (DNA),M13F Genewiz (DNA)5'TR31bp spacer,5'TR(1),5'TR,5'TRcore insul,ES003-1 C->A,YX021-seq-3 (DNA),SrfIhomolog,Added MluI site,PCR,+6EKo279.1 (DNA)homologMluI,MfeI,AgeIAflIIBglIIFseIXZp044.3 (DNA),XZp044.4 (DNA)BsrGImTagBFP2.1 (DNA),Bsu36ImtagBFP2AloIEKo386.1 (DNA),EKo386.2 (DNA)homolog,SalI,WPRE,XZp075.4 (DNA),XZp075.5 (DNA),+3XZp075.6 (DNA)HpaISV40 pA(1),homolog,SV40 pA(1),SV40 pA(1),SV40 pA(1),+3core insul(1) Geneious name: core insul(1),+2EcoRVEco53kI,SacI3'TRES003-3 C->ApLac(F),M13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapIpUCoriR (DNA)

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