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LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHI20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttBsaIBsrDIAmp180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIBsrDIAmp360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattAmp540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataAmp8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaBspHICspCI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggPsiI1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcatt1,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagFspI1,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataaBII(R)5'TR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttBsiWI31bp spacer5'TR(1)5'TR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgccccore insul2,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagacccctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctgggMfeISfiISrfIcore insulSFFV2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cacctgtaggttatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacgtggacatccaataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgBmtINheIEcoRVSFFV2,4602,4802,5002,5202,5402,5602,5802,6002,620
    agatggtcaccgcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttctaccagtggcgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaAflIIBbvCIPpuMIBpu10ISFFV2,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcgcgcgcttctgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGagcgcgcgaagacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCAleIAscIHindIIIVS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG242905-3612M1SFFVmCherryhomo...ch)2,8202,8402,8602,8802,9002,9202,9402,960
    CTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGSV26NG28HE30FE32IE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD822905-3612mCherry2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGSbfIIP84DY86LK88LS90FP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM1402905-3612mCherry3,1603,1803,2003,2203,2403,2603,2803,3003,320
    AGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCABbsIBsgIKasIKasINarINarISfoISfoIBbvCIPflMIPluTIPluTIBpu10IQK142KT144MG146WE148AS150SE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198V2905-3612mCherry3,3403,3603,3803,4003,4203,4403,4603,4803,500
    CAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAAAleIMluIXcmIBsrGISgrAIAflIIIE1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F183622-3720N200IK202LD204IT206SH208NE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS2362905-3612D1A2L3mCherry5' StopGo (2A; Loughran 2017)3,5203,5403,5603,5803,6003,6203,6403,660
    GACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtaCTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatAgeIBaeIKpnIZraIAatIIBamHIAcc65IG1S2G1T2S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T373733-3843T1G2D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333622-3720ADAR substrate3xMS2 SGR5' StopGo (2A; Loughran 2017)5' stem3' stem3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ctagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTgtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAcacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcaPfoISapISpeITliIXhoIBfuAIBspMIBspQIBsrGIPaqCIPpuMIPspXIPaeR7IS1S2E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P333856-3954T1S2VS2KG4EE6LI8KE10NM12HM14KL16YM18EG20TV223961-4668VS2KG4EE6LI8KE10NM12HM14KL16YM18EG20TV223961-46683' StopGo (2A; Loughran 2017)PCR from LSM053mtagBFP23,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatcccccctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggDN24HH26FK28CT30SE32GE34GK36PY38EG40TQ42TM44RI46KV48VE50GG52PL54PF56AF58DI60LA62TS64FL66YG68SK70TF72IN74HT76QG78IP803961-4668DN24HH26FK28CT30SE32GE34GK36PY38EG40TQ42TM44RI46KV48VE50GG52PL54PF56AF58DI60LA62TS64FL66YG68SK70TF72IN74HT76QG78IP803961-4668PCR from LSM053mtagBFP24,0404,0604,0804,1004,1204,1404,1604,1804,200
    gacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagactgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttctPfoIBsu36IDF82FK84QS86FP88EG90FT92WE94RV96TT98YE100DG102GV104LT106AT108QD110TS112LQ114DG116CL118IY120NV122KI124RG126VN128FT130SN132GP134VM136QK1383961-4668DF82FK84QS86FP88EG90FT92WE94RV96TT98YE100DG102GV104LT106AT108QD110TS112LQ114DG116CL118IY120NV122KI124RG126VN128FT130SN132GP134VM136QK1383961-4668PCR from LSM053mtagBFP24,2204,2404,2604,2804,3004,3204,3404,360
    aaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatKT140LG142WE144AF146TE148TL150YP152AD154GG156LE158GR160ND162MA164LK166LV168GG170SH172LI174AN176AK178TT180YR182SK184KP186AK188NL190KM192PG194VY196Y3961-4668KT140LG142WE144AF146TE148TL150YP152AD154GG156LE158GR160ND162MA164LK166LV168GG170SH172LI174AN176AK178TT180YR182SK184KP186AK188NL190KM192PG194VY196Y3961-4668PCR from LSM053mtagBFP24,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAgccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgApaIPacITliIXhoIPspXIPaeR7IPspOMI*1V198DY200RL202ER204IK206EA208NN210ET212YV214EQ216HE218VA220VA222RY224CD226LP228SK230LG232HK234LN2363961-4668V198DY200RL202ER204IK206EA208NN210ET212YV214EQ216HE218VA220VA222RY224CD226LP228SK230LG232HK234LN2363961-4668PCR from LSM053mtagBFP2rrrc-myc epitopehomolog4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtccggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagBclIPmeISalIBGH polyadenylation sequencePolyhi...e tag4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggacgcggccgcaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctgcgccggcgttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcBbsINotIXmnIBGH polyadenylation sequencepSV405, 299 F4,9204,9404,9604,9805,0005,0205,0405,060
    tatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatatacgtttcgtacgtagagttaatcagtcgttggtccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaSexAIpSV405,0805,1005,1205,1405,1605,1805,2005,2205,240
    ggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgatgaaaaagcctgaactcaccgccgactgattaaaaaaaataaatacgtctccggctccggcggagacggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcactactttttcggacttgagtggcBclIPmlIAvrIIMK2KP4EL6T5404-6429pSV40Hygro5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcagctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtZraIAatIIPshAIA8TS10VE12KF14LI16EK18FD20SV22SD24LM26QL28SE30GE32ES34RA36FS38FD40VG42GR44GY46VL48RV50NS52CA54DG56FY58KD60RY62VY64RH665404-6429Hygro5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ctttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgcAsiSIBfuAIBspMIEcoRIFA68SA70AL72PI74PE76VL78DI80GE82FS84ES86LT88YC90IS92RR94AQ96GV98TL100QD102LP104ET106EL108PA110VL112QP114VA116EA118MD120AI122AA1245404-6429Hygro2,...F5,6205,6405,6605,6805,7005,7205,7405,760
    gccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgNdeIAD126LS128QT130SG132FG134PF136GP138QG140IG142QY144TT146WR148DF150IC152AI154AD156PH158VY160HW162QT164VM166DD168TV170SA172SV174AQ176AL178DE180LM1825404-6429Hygro5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacAjuILW184AE186DC188PE190VR192HL194VH196AD198FG200SN202NV204LT206DN208GR210IT212AV214ID216WS218EA220MF222GD224SQ226YE228VA230NI232FF234WR236PW238LA240C5404-6429Hygro5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggSacIIM242EQ244QT246RY248FE250RR252HP254EL256AG258SP260RL262RA264YM266LR268IG270LD272QL274YQ276SL278VD280GN282FD284DA286AW288AQ290GR292CD294AI296VR298S5404-6429Hygro3, 135 F6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaataggtcgacaatcaacctctggattacaaaatttgtgaaagattgactgcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatccagctgttagttggagacctaatgttttaaacactttctaactgacSalIScaIG300AG302TV304GR306TQ308IA310RR312SA314AV316WT318DG320CV322EV324LA326DS328GN330RR332PS334TR336PR338AK340E*3425404-6429HygroWPRE6, 730 R6,3206,3406,3606,3806,4006,4206,4406,460
    gtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgcgttaactaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtcataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgcaattgatttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaBsmIHpaIPsiISV40 pASV40 pA(1)WPRE6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ccaaactcatcaatgtatcttatcatgtctggaattgactcaaatgatgtcaattagtctatcagaagctcatctggtctcccttccgggggacaagacatccctgtttaatatttaaacagcagtgttcccaaactgggttcttatatcccttgctctggtcaaccaggttgcaggtttgagtagttacatagaatagtacagaccttaactgagtttactacagttaatcagatagtcttcgagtagaccagagggaaggccccctgttctgtagggacaaattataaatttgtcgtcacaagggtttgacccaagaatatagggaacgagaccagttggtccaacgtBsaISexAISV40 pASV40 pA(1)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gggtttcctgtcctcacaggaacgaagtccctaaagaaacagtggcagccaggtttagccccggaattgactggattccttttttagggcccattggtatggctttttccccgtatccccccaggtgtctgcaggctcaaagagcagcgagaagcgttcagaggaaagcgatccccccaaaggacaggagtgtccttgcttcagggatttctttgtcaccgtcggtccaaatcggggccttaactgacctaaggaaaaaatcccgggtaaccataccgaaaaaggggcataggggggtccacagacgtccgagtttctcgtcgctcttcgcaagtctcctttcgctagggApaIBstXIEcoNIPspOMIcore insul(1) Geneious name: core insul(1)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gtgccaccttccccgtgcccgggctgtccccgcacgctgccggctcggggatgcggggggagcgccggaccggagcggagccccgggcggctcgctgctgccccctagcgggggagggacgtaattacatccctgggggctttgggggggggctgtccctgatatctataacaagcacggtggaaggggcacgggcccgacaggggcgtgcgacggccgagcccctacgcccccctcgcggcctggcctcgcctcggggcccgccgagcgacgacgggggatcgccccctccctgcattaatgtagggacccccgaaacccccccccgacagggactatagatattgttcSrfIEcoRVcore insul(1) Geneious name: core insul(1)7,0207,0407,0607,0807,1007,1207,1407,160
    aaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaaaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaattttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccc7,1807,2007,2207,2407,2607,2807,3007,3207,340
    agctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatatttcaagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgtcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcBsmI3'TR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    cccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttctcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgcca7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgcttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggcttcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgcBsgI7,7207,7407,7607,7807,8007,8207,8407,860
    aggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggpLac(F)7,8807,9007,9207,9407,9607,9808,0008,0208,040
    taatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggcattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgSapIBspQI8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaaacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttPciIAflIII8,2408,2608,2808,3008,3208,3408,3608,3808,400
    tcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccata8,4208,4408,4608,4808,5008,5208,5408,560
    ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatc8,5808,6008,6208,6408,6608,6808,7008,7208,740
    gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatcgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtcta8,7608,7808,8008,8208,8408,8608,8808,9008,920
    tacgcgcagaaaaaaaggatctcaaatgcgcgtctttttttcctagagtt8,9308,9408,950
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8950 bp65007000750080008500500100015002000core insul5'TR(1)AmppLac(F)EKo152.2 (DNA)oLSM0043 (DNA)EKbR (DNA)pNSK053 (DNA)pNK018 (DNA)bghF (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)BspHI,BsaI,BsrDIFspI,AclI,BsrDIScaI,BcgIAclIXmnIBspHICspCIPsiIFrtF (DNA)FlpInR (DNA)FspIM13-40FOR Genewiz (DNA)M13F Genewiz (DNA)BII(R),5'TRBsiWI31bp spacer5'TR5'TRBsaISexAIEcoNI,PspOMI,ApaI,BstXIEWXglobF NONSPECIFIC DONT USE (DNA),SrfIcore insul(1) Geneious name: core insul(1)EcoRVBsmI3'TRES003-3 C->ABsgIM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA),AflIII,PciIpUCoriR (DNA)

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    8950

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