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pEevee-iAkt-NES
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattPvuISpeICAG promoter20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoter180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccNcoIBsaAISnaBICAG promoter360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPspOMICAG promoter720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagISacIICAG promoter8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccgBlpICAG promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggtMreINaeIBfuAIBspMIPaqCISgrAIBstAPINgoMIVCAG promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aatctggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagcctcggggctgccgcagggggacggctgccttttagaccgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgcccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagaggtagaggtcggagccccgacggcgtccccctgccgacggaaKasIKasINaeINarINarISfoISfoIPluTIPluTINgoMIVCAG promoter1,4201,4401,4601,4801,5001,5201,5401,560
    cgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttgttgtgctgtctcatcattttggcaagaattcggcATGGTGAGCAAGGGCGgccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatcgatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaacaacacgacagagtagtaaaaccgttcttaagccgTACCACTCGTTCCCGCEcoRICAG promoterCAG1640YPet1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTTCTATGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCGAAGATACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGHindIIIYPet1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CCTGGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACGGACCCGATGCCGGACGTCACGAAGCGGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGPasIYPet1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTGGCGGCTGTTCGTCTTCTTGCCGTAGTTCCGGTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGCCGCACGTCGAGCGGCYPet2,1202,1402,1602,1802,2002,2202,2402,260
    ACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGTTCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCCTGACCGCCGCCGGGATCACTGAGGGCATGAACGAGCTGTACCTTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGATGGTCAGGCGGGACAAGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGGACTGGCGGCGGCCCTAGTGACTCCCGTACTTGCTCGACATGGAPsrITliIXhoIBpu10IPaeR7IYPet2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CGAGAAGTTTTCTCAAGAACAGATCGGCGAAAACATTGTGTGCAGGGTCATTTGTACCACGGGTCAAATTCCCATCCGAGATTTGTCAGCTGATATTTCACAAGTGCTTAAGGAAAAACGATCCATAAAGAAAGTTTGGACATTTGGTAGAAACCCAGCCTGTGACTATCATTTAGCTCTTCAAAAGAGTTCTTGTCTAGCCGCTTTTGTAACACACGTCCCAGTAAACATGGTGCCCAGTTTAAGGGTAGGCTCTAAACAGTCGACTATAAAGTGTTCACGAATTCCTTTTTGCTAGGTATTTCTTTCAAACCTGTAAACCATCTTTGGGTCGGACACTGATAGTAAATAflIIBseYIFHA1 domain2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GGAAACATTTCAAGACTGTCAAATAAGCATTTCCAAATACTACTAGGAGAAGACGGTAACCTTTTATTGAATGACATTTCCACTAATGGGACCTGGTTAAATGGGCAAAAAGTCGAGAAGAACAGCAATCAGTTACTGTCCCAAGGTGATGAAATAACCGTTGGTGTAGGCGTGGCCTTTGTAAAGTTCTGACAGTTTATTCGTAAAGGTTTATGATGATCCTCTTCTGCCATTGGAAAATAACTTACTGTAAAGGTGATTACCCTGGACCAATTTACCCGTTTTTCAGCTCTTCTTGTCGTTAGTCAATGACAGGGTTCCACTACTTTATTGGCAACCACATCCGCACCAjuIBbsIXmnIPpuMISexAIBstEIIFHA1 domain2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AATCAGATATTTTATCTCTGGTCATTTTCATAAACGACAAATTTAAGCAGTGCCTGGAGCAGAACAAAGTTGATCGCggtaccAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTtccggcAGTGCTGGTGGTAGTGCTGGTGGTagTTAGTCTATAAAATAGAGACCAGTAAAAGTATTTGCTGTTTAAATTCGTCACGGACCTCGTCTTGTTTCAACTAGCGccatggTCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCAaggccgTCACGACCACCATCACGACCACCAtcKpnIAcc65ISA2GG4SA6GG8SA10GG12SA14GG16SA18GG20SG22SA24GG26SA28GG30S2884-3231EV linkerFHA1 domain2,8202,8402,8602,8802,9002,9202,9402,960
    taccAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTtccggcAGTGCTGGTGGTAGTGCTGGTGGTagtaccAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTtccggcAGTGCTGGTatggTCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCAaggccgTCACGACCACCATCACGACCACCAtcatggTCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCAaggccgTCACGACCAT32SA34GG36SA38GG40SA42GG44SA46GG48SA50GG52SG54SA56GG58SA60GG62ST64SA66GG68SA70GG72SA74GG76SA78GG80SA82GG84SG86SA88G2884-3231EV linker2,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGTAGTGCTGGTGGTagtaccAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTAGTGCTGGTGGTtccggaAGCGGCAGACCCAGAACCACCACCTTCGCCGACAGCTGCAAGCCCggcggccgcATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGCCATCACGACCACCAtcatggTCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCATCACGACCACCAaggcctTCGCCGTCTGGGTCTTGGTGGTGGAAGCGGCTGTCGACGTTCGGGccgccggcgTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCEagINotIBspEIG90SA92GG94ST96SA98GG100SA102GG104SA106GG108SA110GG112SA114GG1162884-3231EV linkerAkt substratenTurqupise-GL3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGTCCTGGGGCGTGCAACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGGACAGGACCCCGCACGTDrdInTurqupise-GL3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCCACGAAGCGGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGnTurqupise-GL3,5203,5403,5603,5803,6003,6203,6403,660
    ATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCGGGAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACATAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTAGTCGCCCTTGCAGATATAGTGGCGGCTGTTCGTCTTCTTGCCGTAGTTCCGGTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGCCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTnTurqupise-GL3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCTTAAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCTTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGtctagaCTGCAGCTGCCTCCTCTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGAATTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGAACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACagatctGACGTCGACGGAGGAGAAhdIXbaIAflIIBpu10InTurqupise-GLNES3,8603,8803,9003,9203,9403,9603,9804,0004,020
    GGAACGCCTGACTCTGGATTAAGTCGACggatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcCCTTGCGGACTGAGACCTAATTCAGCTGcctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacacagagagtgagccttcctgtataccctcccgAccISalIHincIIRabbit b-globin 3'-flanking sequenceCAGGS-R2NES4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgccatatgctggctgccatgaacaaaggtggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagatttttttttatttagtaaattttgtagtcttactcataaaccaaatctcaaaccgttgtatacggtatacgaccgacggtacttgtttccaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcttttcggaactgaactccaatctaaaaaaaaatAjuIPflMIBstAPIRabbit b-globin 3'-flanking sequence4,2204,2404,2604,2804,3004,3204,3404,360
    tattttgtttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatgaagatccctcgacctgcagcccaagcttggcgtaatcatggtcatagctgtttccataaaacaaacacaataaaaaaagaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacttctagggagctggacgtcgggttcgaaccgcattagtaccagtatcgacaaaggNspIPciIBfuAIBspMIAflIIIHindIIIRabbit b-globin 3'-flanking sequenceM13-rev4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgcctaggcgtagagttaatcagtcBamHIBsaBIlac promoterLacOSV40 Ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    caaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttggttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacBplIBplINcoISfiIStuIAvrIISV40 Ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    caaaaagctaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggtttttcgattgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctaggcgacgtaattacttagccggttgcgcgcccBsmIPsiIBamHISV40 Ori4,9204,9404,9604,9805,0005,0205,0405,060
    gagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggNspIPciISapIBsaXIBspQIAflIII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    aggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgDrdIColE1 origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaaaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttApaLIBciVIBseYIColE1 origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    cccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctColE1 origin5,6205,6405,6605,6805,7005,7205,7405,760
    gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagatcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaColE1 origin5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatAhdIAmpRAmp_R5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggccBsaIAmpRAmp_R6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcgcttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagcAclIFspIAmpRAmp_R6,3206,3406,3606,3806,4006,4206,4406,460
    gtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggPvuIScaIAmpRAmp_R6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaAclIXmnIApaLIAmp_RAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccSspIBciVIAmp_R6,8406,8606,8806,9006,9206,9406,9606,9807,000
    ttccgcgcacatttccccgaaaagtgccacctgaaggcgcgtgtaaaggggcttttcacggtggacSexAI7,020
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7033 bp500055006000650050010001500YPetCAG1640AmpRPvuISpeI,BsaAI,SnaBI,NcoIAfeICAG promoter,PspOMI,ApaI,AfeIEagI,SacIIBlpI,BfuAI,BspMI,PaqCIMreI,NgoMIV,SgrAI,NaeI,BstAPIKasINarI,SfoI,PluTI,KasI,NarI,SfoI,NgoMIV,PluTI,NaeIEcoRIDrdIBciVIBseYIApaLIColE1 originAhdI,BsaIFspI,AclIAmp_R,PvuI,ScaIAclI,XmnI,ApaLI,SspI,BciVI,SexAI

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    7033