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LSM102 CMV-TO-CX3CR1-10aa-PCP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggfrom LSM040pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttBmrIBsaIAmp (bla)from LSM040180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspINmeAIIIAmp (bla)from LSM040360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIAmp (bla)from LSM040540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIXmnIAmp (bla)from LSM040720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataAcuISspIAmp (bla)bla promoterfrom LSM0408809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactSgrDIbla promoterfrom LSM0401,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIBsiWIAflIIIBpu10Ifrom LSM0401,2401,2601,2801,3001,3201,3401,3601,3801,400
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatcccSpeICMVCMV promoter (with TetOs)from LSM0401,4201,4401,4601,4801,5001,5201,5401,560
    actttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatcttgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagaBmrINdeICMVCMV promoter (with TetOs)from LSM0401,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgcccctgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggCspCISnaBICMVCMV promoter (with TetOs)from LSM0401,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacactaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtgSacISacISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetOfrom LSM0401,9401,9601,9802,0002,0202,0402,0602,0802,100
    cgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccATGGATCAATTTCCAGAAAGTGTGACTGAAAATTTTGAGTATGACGACCTGGCAGAGGCCTGCTACATCGGCGATATTGTTGTCTTCGGCACTGTTTTCCTGAGCATTTTCTATTCCGTTATAgccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggTACCTAGTTAAAGGTCTTTCACACTGACTTTTAAAACTCATACTGCTGGACCGTCTCCGGACGATGTAGCCGCTATAACAACAGAAGCCGTGACAAAAGGACTCGTAAAAGATAAGGCAATATPmeIStuIAflIIEcoNIBpu10IHindIIIMD2QF4PE6SV8TE10NF12EY14DD16LA18EA20CY22IG24DI26VV28FG30TV32FL34SI36FY38SV40I2153-4099from LSM068Original CX3CX3CR1CMV pro...TetOs)homologfrom LSM0402,1202,1402,1602,1802,2002,2202,2402,260
    TTCGCCATTGGCCTCGTTGGAAATCTTCTGGTGGTGTTCGCGCTCACCAATAGTAAGAAGCCTAAAAGCGTGACAGACATTTATCTTCTTAACCTCGCATTGTCAGACTTGTTATTTGTCGCCACCCTGCCATTTTGGACCCACTATTTAATTAATGAGAAGGGACTTCACAACGAAGCGGTAACCGGAGCAACCTTTAGAAGACCACCACAAGCGCGAGTGGTTATCATTCTTCGGATTTTCGCACTGTCTGTAAATAGAAGAATTGGAGCGTAACAGTCTGAACAATAAACAGCGGTGGGACGGTAAAACCTGGGTGATAAATTAATTACTCTTCCCTGAAGTGTTGCPacIF42AI44GL46VG48NL50LV52VF54AL56TN58SK60KP62KS64VT66DI68YL70LN72LA74LS76DL78LF80VA82TL84PF86WT88HY90LI92NE94KG96LH98N2153-4099from LSM068Original CX3CX3CR12,2802,3002,3202,3402,3602,3802,4002,4202,440
    CGATGTGTAAATTTACTACAGCCTTCTTCTTCATAGGGTTCTTTGGGTCAATCTTCTTTATAACAGTCATATCAATTGATAGATATCTGGCCATCGTTCTGGCTGCCAACAGCATGAATAATCGGACAGTGCAACATGGTGTAACTATTAGTCTGGGCGTATGGGCCGCCGCTATGCTACACATTTAAATGATGTCGGAAGAAGAAGTATCCCAAGAAACCCAGTTAGAAGAAATATTGTCAGTATAGTTAACTATCTATAGACCGGTAGCAAGACCGACGGTTGTCGTACTTATTAGCCTGTCACGTTGTACCACATTGATAATCAGACCCGCATACCCGGCGGCGATAMfeIMscIBstXIEcoRVA100MC102KF104TT106AF108FF110IG112FF114GS116IF118FI120TV122IS124ID126RY128LA130IV132LA134AN136SM138NN140RT142VQ144HG146VT148IS150LG152VW154AA156AI1582153-4099from LSM068Original CX3CX3CR12,4602,4802,5002,5202,5402,5602,5802,6002,620
    TTTAGTGGCCGCTCCCCAATTCATGTTTACCAAACAAAAGGAAAACGAATGCTTGGGAGATTATCCAGAAGTACTGCAGGAAATTTGGCCCGTACTCAGAAACGTGGAGACAAACTTTCTTGGGTTTCTGTTGCCACTTCTGATTATGTCCTATTGCTACTTTAGGATAATCCAAAAATCACCGGCGAGGGGTTAAGTACAAATGGTTTGTTTTCCTTTTGCTTACGAACCCTCTAATAGGTCTTCATGACGTCCTTTAAACCGGGCATGAGTCTTTGCACCTCTGTTTGAAAGAACCCAAAGACAACGGTGAAGACTAATACAGGATAACGATGAAATCCTATTAGGTTBsmIPstILV160AA162PQ164FM166FT168KQ170KE172NE174CL176GD178YP180EV182LQ184EI186WP188VL190RN192VE194TN196FL198GF200LL202PL204LI206MS208YC210YF212RI214IQ2162153-4099from LSM068Original CX3CX3CR12,6402,6602,6802,7002,7202,7402,7602,7802,800
    ACCCTCTTTAGCTGTAAGAATCACAAGAAAGCCAAAGCTATCAAGCTCATATTACTGGTAGTAATTGTCTTCTTTCTCTTCTGGACGCCTTATAATGTCATGATTTTCTTGGAGACACTCAAGCTCTATGATTTCTTTCCATCTTGCGATATGCGCAAGGATTTGAGGCTTGCTCTGGGAGAAATCGACATTCTTAGTGTTCTTTCGGTTTCGATAGTTCGAGTATAATGACCATCATTAACAGAAGAAAGAGAAGACCTGCGGAATATTACAGTACTAAAAGAACCTCTGTGAGTTCGAGATACTAAAGAAAGGTAGAACGCTATACGCGTTCCTAAACTCCGAACGAGBplIFspITL218FS220CK222NH224KK226AK228AI230KL232IL234LV236VI238VF240FL242FW244TP246YN248VM250IF252LE254TL256KL258YD260FF262PS264CD266MR268KD270LR272LA2742153-4099from LSM068Original CX3CX3CR12,8202,8402,8602,8802,9002,9202,9402,960
    TGTCTGTAACAGAAACAGTTGCTTTCAGTCACTGCTGCTTAAACCCGTTGATCTATGCATTTGCCGGAGAAAAGTTCAGGCGTTATCTCTACCATCTTTATGGCAAGTGCCTTGCGGTGCTCTGCGGTCGGTCCGTCCATGTCGATTTCAGCTCCAGTGAAAGTCAAAGATCCAGACAGACATTGTCTTTGTCAACGAAAGTCAGTGACGACGAATTTGGGCAACTAGATACGTAAACGGCCTCTTTTCAAGTCCGCAATAGAGATGGTAGAAATACCGTTCACGGAACGCCACGAGACGCCAGCCAGGCAGGTACAGCTAAAGTCGAGGTCACTTTCAGTTTCTAGGTCBplINsiIBstXIRsrIILS276VT278ET280VA282FS284HC286CL288NP290LI292YA294FA296GE298KF300RR302YL304YH306LY308GK310CL312AV314LC316GR318SV320HV322DF324SS326SE328SQ330RS332R2153-4099from LSM068Original CX3CX3CR12,9803,0003,0203,0403,0603,0803,1003,1203,140
    ACACGGGAGCGTTTTGAGTTCCAACTTCACTTACCACACCTCTGATGGTGATGCTCTGCTCCTGCTGACAGGCGGTCGCACTCCCCCTAGCCTCGGTCCACAGGATGAGTCATGCACGACCGCGAGTAGCTCCCTCGCCAAAGACACTTCTTCCGGTACCGGTGGCGGATCCGGTTGTGCCCTCGCAAAACTCAAGGTTGAAGTGAATGGTGTGGAGACTACCACTACGAGACGAGGACGACTGTCCGCCAGCGTGAGGGGGATCGGAGCCAGGTGTCCTACTCAGTACGTGCTGGCGCTCATCGAGGGAGCGGTTTCTGTGAAGAAGGCCATGGCCACCGCCTAGGCCAAgeIKpnIXcmIBamHIAcc65IH334GS336VL338SS340NF342TY344HT346SD348GD350AL352LL354LT356GG358RT360PP362SL364GP366QD368ES370CT372TA374SS376SL378AK380DT382SS384GT386GG388GS390G2153-4099G1T2G3G4G5S6G73305-3334from LSM068CX3CR1v2Original CX3short linkerfrom LSM0403,1603,1803,2003,2203,2403,2603,2803,3003,320
    GGCGGGGCTCTAGCTTCTAAAACAATAGTGTTGTCTGTAGGAGAAGCCACCAGAACCCTCACGGAGATCCAAAGCACAGCGGATAGACAAATTTTCGAAGAGAAAGTGGGACCGCTTGTCGGAAGACTCAGGTTGACAGCTTCCCTGCGGCAGAATGGCGCTAAGACCGCTTATACCGCCCCGAGATCGAAGATTTTGTTATCACAACAGACATCCTCTTCGGTGGTCTTGGGAGTGCCTCTAGGTTTCGTGTCGCCTATCTGTTTAAAAGCTTCTCTTTCACCCTGGCGAACAGCCTTCTGAGTCCAACTGTCGAAGGGACGCCGTCTTACCGCGATTCTGGCGAATATBstBIG392GA394LA396SK398TI400VL402SV404GE406AT408RT410LT412EI414QS416TA418DR420QI422FE424EK426VG428PL430VG432RL434RL436TA438SL440RQ442NG444AK446TA448Y2153-4099G8G9A10PCPfrom LSM0403,3403,3603,3803,4003,4203,4403,4603,4803,500
    GGGTGAATCTCAAACTTGATCAAGCAGATGTTGTTGATAGCGGCTTGCCCAAAGTACGATATACTCAAGTTTGGTCACATGATGTGACAATCGTTGCAAACTCAACGGAAGCGAGTAGGAAAAGCCTTTATGATCTCACTAAGTCACTCGTCGCCACCTCTCAAGTTGAGGACCTCCCACTTAGAGTTTGAACTAGTTCGTCTACAACAACTATCGCCGAACGGGTTTCATGCTATATGAGTTCAAACCAGTGTACTACACTGTTAGCAACGTTTGAGTTGCCTTCGCTCATCCTTTTCGGAAATACTAGAGTGATTCAGTGAGCAGCGGTGGAGAGTTCAACTCCTGGABclIPpuMIR450VN452LK454LD456QA458DV460VD462SG464LP466KV468RY470TQ472VW474SH476DV478TI480VA482NS484TE486AS488RK490SL492YD494LT496KS498LV500AT502SQ504VE506DL5082153-4099PCPfrom LSM0403,5203,5403,5603,5803,6003,6203,6403,660
    TGTCGTCAATTTGGTGCCTCTTGGTCGGGGAGGCGGTTCCGGCGGGGGTACCCTTGCCAGTAAGACCATCGTGTTGAGCGTGGGAGAGGCAACCCGCACACTGACAGAAATCCAGTCAACAGCAGACAGACAGATTTTTGAGGAGAAAGTGGGTCCGCTTGTCGGGCGGCTGCGGACAGCAGTTAAACCACGGAGAACCAGCCCCTCCGCCAAGGCCGCCCCCATGGGAACGGTCATTCTGGTAGCACAACTCGCACCCTCTCCGTTGGGCGTGTGACTGTCTTTAGGTCAGTTGTCGTCTGTCTGTCTAAAAACTCCTCTTTCACCCAGGCGAACAGCCCGCCGACGCCKpnIBseRIAcc65IVV510NL512VP514LG516RG518GG520SG522GG524TL526AS528KT530IV532LS534VG536EA538TR540TL542TE544IQ546ST548AD550RQ552IF554EE556KV558GP560LV562GR564LR5662153-4099PCPPCPfrom LSM0403,6803,7003,7203,7403,7603,7803,8003,8203,840
    CTGACGGCTTCATTGCGACAGAACGGAGCCAAAACTGCCTATCGAGTCAACCTTAAACTCGATCAAGCGGATGTTGTGGATTCCGGTCTGCCAAAAGTACGGTACACGCAGGTGTGGTCCCATGACGTGACCATTGTGGCGAACAGTACCGAGGCGTCTCGCAAAAGCCTTTACGGACTGCCGAAGTAACGCTGTCTTGCCTCGGTTTTGACGGATAGCTCAGTTGGAATTTGAGCTAGTTCGCCTACAACACCTAAGGCCAGACGGTTTTCATGCCATGTGCGTCCACACCAGGGTACTGCACTGGTAACACCGCTTGTCATGGCTCCGCAGAGCGTTTTCGGAAATGCAleIBmgBIPaqCILT568AS570LR572QN574GA576KT578AY580RV582NL584KL586DQ588AD590VV592DS594GL596PK598VR600YT602QV604WS606HD608VT610IV612AN614ST616EA618SR620KS622LY6242153-4099PCPfrom LSM0403,8603,8803,9003,9203,9403,9603,9804,0004,020
    ATTTGACCAAATCACTCGTTGCAACTTCACAGGTGGAAGACCTGGTTGTCAATCTTGTACCTTTGGGCAGGtgagccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcTAAACTGGTTTAGTGAGCAACGTTGAAGTGTCCACCTTCTGGACCAACAGTTAGAACATGGAAACCCGTCCactcggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagApaIAvaIBclIPmeITliIXhoIBseRIBsoBIPaqCIPspXISexAIPaeR7IPspOMIDL626TK628SL630VA632TS634QV636ED638LV640VN642LV644PL646GR648*2153-4099PCPc-myc epitopePolyhi...e tagfrom LSM0404,0404,0604,0804,1004,1204,1404,1604,1804,200
    agcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagctcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgBGH polyadenylation sequencefrom LSM0404,2204,2404,2604,2804,3004,3204,3404,360
    aagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcggttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgccClaINspISphIBspDIPvuIIBGH polyadenylation sequenceBGH pAfrom LSM0404,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttKflINaeIPpuMINgoMIVfrom LSM0404,5604,5804,6004,6204,6404,6604,6804,7004,720
    cttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcggaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcBarIMscIXbaIBsaXIPshAIHygromycinFRTfrom LSM0404,7404,7604,7804,8004,8204,8404,8604,8804,900
    aagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacEcoRIHygromycinfrom LSM0404,9204,9404,9604,9805,0005,0205,0405,060
    catctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcaPstIPvuIAsiSIRsrIIBstAPIDraIIIHygromycinfrom LSM0405,0805,1005,1205,1405,1605,1805,2005,2205,240
    gatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggctaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgccNdeIBstAPIDraIIINmeAIIIHygromycinfrom LSM0405,2605,2805,3005,3205,3405,3605,3805,4005,420
    acaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatAjuISacIIHygromycinfrom LSM0405,4405,4605,4805,5005,5205,5405,5605,5805,600
    tatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaaatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttHygromycinfrom LSM0405,6205,6405,6605,6805,7005,7205,7405,760
    gtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttctcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaNaeIPfoINgoMIVHygromycinfrom LSM0405,7805,8005,8205,8405,8605,8805,9005,9205,940
    tcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcatBsmIBstZ17ISV40 pAfrom LSM0405,9605,9806,0006,0206,0406,0606,0806,1006,120
    atcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgtagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacPvuIIfrom LSM0406,1406,1606,1806,2006,2206,2406,2606,2806,300
    cattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacagtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtPciISapIBsaXIBspQIAflIIIfrom LSM0406,3206,3406,3606,3806,4006,4206,4406,460
    tgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcaNspIfrom LSM040pUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    gcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaatfrom LSM040pUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgcaggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgfrom LSM040pUC ori6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttAcuIfrom LSM040pUC ori7,0207,0407,0607,0807,1007,120
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7122 bp500055006000650050010001500from LSM040HygromycinCMV promoter (with TetOs)CMVSV40 pAbla promoterAmp (bla)pUC oriEKo152.2 (DNA)oLSM0283 (DNA)XZp025.1 (DNA)EKbF (DNA)pNK018 (DNA)EKo326.3 (DNA)oLSM-pUC_ori_R (DNA)pUCoriR (DNA)BmrI,BsaINmeAIII,FspI,AclIPvuIBcgIAclI,XmnIAcuISspISgrDIFlpInF1 (DNA)FlpInF (DNA),FlpInF2 (DNA),EKo152.4 (DNA),MfeI,Bpu10IEKo279.1 (DNA),EKo272.1 (DNA),BsiWI,AflIII,MluI,SpeIEK_CMVFa (DNA)EK_CMVFb (DNA)EK_CMVFc (DNA)EK_CMVFd (DNA),NdeI,BmrISnaBIEK_CMVFe (DNA)NmeAIIIBstAPI,DraIIIAjuISacIIHyg_F (DNA)PfoI,NgoMIV,NaeISV40PA_R (DNA)SV40pA-R Genewiz (DNA),BsmIBstZ17IM13R Genewiz (DNA),M13-48REV Genewiz (DNA)PvuII,BsaXI,BspQI,SapIpUCori5F (DNA),AflIII,PciI,NspIoLSM-pUC_ori_F (DNA)AcuI

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    7122