Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pGS21a_GGA_430
  • Sequence Map
    Editing disabled on read-only sequence.
    ACCCAACACGGAGGTTTACGAGCTGCCGGTAGAAAAATTCTTGGAAGTCGCGACCGTGCGTGTAGAGAAAGACGAAGATCAGGAGGAACAGACTGAAGCCCCAGAAGAACAGGTCGCTGAATAACTACAGACCTTAAGGAGGAACTACGATGGAGAACACCAATATCGTCAAAGCTGGGTTGTGCCTCCAAATGCTCGACGGCCATCTTTTTAAGAACCTTCAGCGCTGGCACGCACATCTCTTTCTGCTTCTAGTCCTCCTTGTCTGACTTCGGGGTCTTCTTGTCCAGCGACTTATTGATGTCTGGAATTCCTCCTTGATGCTACCTCTTGTGGTTATAGCAGTTTCGNruIAflIISSBPRBSTPBPDSBP620406080100120140160
    AACGTTTGACACGGAAACCCTGGAAGGACAGATCAAAATTTTTAATGCTCAGACAGGCGGCGGACAGTCGTTCAAAAATTTGCCGGATGGCACCATTATAGAAGCCAACGCGATTGCGCAATATAAACAGGTGAGCGATACATACGGGGACGCGAAGGAGGAAACGGTTACCACTTTGCAAACTGTGCCTTTGGGACCTTCCTGTCTAGTTTTAAAAATTACGAGTCTGTCCGCCGCCTGTCAGCAAGTTTTTAAACGGCCTACCGTGGTAATATCTTCGGTTGCGCTAACGCGTTATATTTGTCCACTCGCTATGTATGCCCCTGCGCTTCCTCCTTTGCCAATGGTGABstEIISSBPTPBP180200220240260280300320340
    ATTTTTGCGGCGGATGGTTCGCTGTATAGCGCTATCAGTAAAACTGTCGCAGAAGCCGCATCTGATCTGATTGACCTTGTGACCCGCCATAAACTCGAGACGTTTAAGGTTAAAGTGGTACAGGGTACGTCAAGTAAAGGTAACGTATTCTTCAGCTTACAGCTGTCCCTGTAAcTAAAAACGCCGCCTACCAAGCGACATATCGCGATAGTCATTTTGACAGCGTCTTCGGCGTAGACTAGACTAACTGGAACACTGGGCGGTATTTGAGCTCTGCAAATTCCAATTTCACCATGTCCCATGCAGTTCATTTCCATTGCATAAGAAGTCGAATGTCGACAGGGACATTgCspCISSBPTPBP360380400420440460480500520
    tgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgacgattgtttcgggctttccttcgactcaaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctaaccgcttaccctgcgcgggacatcgccgcgtaattcgcgccgcccacBlpIStyIT7 terminatorf1 oriT7 Term540560580600620640660680700
    tggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaactaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaf1 oriF1ori-R720740760780800820840860
    tgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctatactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaatattccctaaaacggctaaagccggataAloIPsiIDraIIIf1 oriF1ori-F8809009209409609801,0001,0201,040
    tggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgctaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaSspICspCIAmpR promoterf1 ori1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgSspIBssSIBssSαIAmpRAmpR promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    agcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaatcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtctttScaIAmpRAmp-R1,4201,4401,4601,4801,5001,5201,5401,560
    agcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagcttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgaPvuIAmpR1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctcttacttcggtatggtttgctgctcgcactgtggtgctacggacgtcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaPstINmeAIIIAmpR1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaatAhdIBsaIAmpR1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggtcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttccAmpR2,1202,1402,1602,1802,2002,2202,2402,260
    atcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttatagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaggaagatcacatcggcatcaatori2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccaccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtAlwNIori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacaBssSIBssSαIoripBR322ori-F2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggPciIori2,8202,8402,8602,8802,9002,9202,9402,960
    gcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcaatggtgcactctcagtacaatctgctctgatgccgcatagttaagcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgttaccacgtgagagtcatgttagacgagactacggcgtatcaattcSapIBspQIbompRS-markerL44402,9803,0003,0203,0403,0603,0803,1003,1203,140
    ccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgPflFIBstZ17ITth111IbomroppGEX 3'3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaaggacaaaccagtgactacggaggcarop3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgccattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctctttttagtgagtcccagttacggtcgcg3,5203,5403,5603,5803,6003,6203,6403,660
    ttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcgtctgcaaaacgtcgtcgtcagcg3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccccggcggtacggccgctattaccggacgaagagcggctttgcaaaccaccgccctggtcactgcttccgFspAIPpuMI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgccaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgccgctgctatcagtacggggcgcggPshAI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    caccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggttgtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaalacI4,2204,2404,2604,2804,3004,3204,3404,360
    tgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgccgccctatattgtactcgacagaaHpaIHincIIlacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cggtatcgtcgtatcccactaccgagatgtccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccatagcagcatagggtgatggctctacaggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacttttggcctgtaccgtgaggtcaglacI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaacggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcagcgcatggcagaagtaccctctttApaIBstEIIPspOMINmeAIIIlacI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgctattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcggcgaaatgtccgaagctgcggcgBclIMluIlacI4,9204,9404,9604,9805,0005,0205,0405,060
    ttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctcaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtgcgccaacccttacattaagtcgaglacI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccatagcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagagaaggcccgcgatagtacggtatBstAPIlacIlacI promoterLacI-R5,2605,2805,3005,3205,3405,3605,3805,4005,420
    ccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgtcagggggccggtgccccggacggSphIPflMIlacI promoter5,4405,4605,4805,5005,5205,5405,5605,5805,600
    accatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatcgatctcgatcccgcgaaattaatactggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagctagagctagggcgctttaattatgClaIBspDISgrAIpBRrevBam5,6205,6405,6605,6805,7005,7205,7405,760
    gactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatctgagtgatatccccttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatgtatacaggggatatgatccaataaccttttaattcccggaacacgttgggtgagctgaagaaaaccttatagaacttctttttatacttctcgtaBarINdeISapIXbaIBspQIMS2PI4LG6YW8KI10KG12LV14QP16TR18LL20LE22YL24EE26KY28EE30H5858-8341GSTGST-DNAPol-6xHislac operatorefficien..., 1989)T7T7 ...er5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgaacatactcgcgctacttccactatttaccgctttgtttttcaaacttaacccaaacctcaaagggttagaaggaataatataactaccactacaatttaattgtgtcagataccggtagtatgcaatatatcgactgttcgtgttgtacaacccaccaacaggttttctcgcacMscIPciIL32YE34RD36EG38DK40WR42NK44KF46EL48GL50EF52PN54LP56YY58ID60GD62VK64LT66QS68MA70II72RY74IA76DK78HN80ML82GG84CP86KE88R5858-8341GSTGST-DNAPol-6xHis5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtgtctctaaagttacgaacttcctcgccaaaacctataatctatgccacaaagctcttaacgtatatcatttctgaaactttgagagtttcaactaaaagaatcgttcgatggactttacgacttttacaagcttctagcaaatacagtattttgtataaatttaccactagtacaBclISwaIA90EI92SM94LE96GA98VL100DI102RY104GV106SR108IA110YS112KD114FE116TL118KV120DF122LS124KL126PE128ML130KM132FE134DR136LC138HK140TY142LN144GD146HV1485858-8341GSTGST-DNAPol-6xHis6,1406,1606,1806,2006,2206,2406,2606,2806,300
    aacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggttgggtaggactgaagtacaacatactgcgagaactacaacaaaatatgtacctgggttacacggacctacgcaagggttttaatcaaacaaaattttttgcataacttcgatagggtgtttaactattcatgaactttaggtcgttcatatatcgtaccggaaacgtcccgaccScaITH150PD152FM154LY156DA158LD160VV162LY164MD166PM168CL170DA172FP174KL176VC178FK180KR182IE184AI186PQ188ID190KY192LK194SS196KY198IA200WP202LQ204GW2065858-8341GSTGST-DNAPol-6xHis6,3206,3406,3606,3806,4006,4206,4406,460
    caagccacgtttggtggtggcgaccatcctccaaaatcggatctgggccacacaggccatagatctggtaccgacgacgacgacaaggccatggctgatatcggatccatgccgcgtaaaatgtattcctgtgattttgaaacgacgacgaaagttgaagattgccgtgtgtggggttcggtgcaaaccaccaccgctggtaggaggttttagcctagacccggtgtgtccggtatctagaccatggctgctgctgctgttccggtaccgactatagcctaggtacggcgcattttacataaggacactaaaactttgctgctgctttcaacttctaacggcacacacccKpnINcoISfiIStyIBamHIBglIIAcc65IQA208TF210GG212GD214HP216PK218SD220LG222HT224GH226RS228GT230DD232DD234KA236MA238DI240GS242MP244RK246MY248SC250DF252ET254TT256KV258ED260CR262VW2645858-8341GST-DNAPol-6xHisGSTpGEX 5'ente...ite6,4806,5006,5206,5406,5606,5806,6006,6206,640
    cgtatggttatatgaacatcgaagaccattcagaatataaaattggcaactcgctggatgaatttatggcgtgggtgctgaaagttcaggccgacctgtacttccacaatctgaaatttgatggtgcgttcattatcaactggctggaacgtaatggctttaaatggagcgccgagcataccaatatacttgtagcttctggtaagtcttatattttaaccgttgagcgacctacttaaataccgcacccacgactttcaagtccggctggacatgaaggtgttagactttaaactaccacgcaagtaatagttgaccgaccttgcattaccgaaatttacctcgcggctAY266GY268MN270IE272DH274SE276YK278IG280NS282LD284EF286MA288WV290LK292VQ294AD296LY298FH300NL302KF304DG306AF308II310NW312LE314RN316GF318KW320SA322D5858-8341GST-DNAPol-6xHis6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tggtctgccgaacacctataatacgattatctctcgtatgggccaatggtatatgattgatatctgcctgggctacaaaggtaaacgcaaaattcataccgtgatctatgacagcctgaaaaaactgccgtttccggtgaagaaaattgcgaaagatttcaaactgaccgtcctgaccagacggcttgtggatattatgctaatagagagcatacccggttaccatatactaactatagacggacccgatgtttccatttgcgttttaagtatggcactagatactgtcggacttttttgacggcaaaggccacttcttttaacgctttctaaagtttgactggcaggacG324LP326NT328YN330TI332IS334RM336GQ338WY340MI342DI344CL346GY348KG350KR352KI354HT356VI358YD360SL362KK364LP366FP368VK370KI372AK374DF376KL378TV380L5858-8341GST-DNAPol-6xHis6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aaaggcgatattgactatcacaaagaacgtccggttggttacaaaatcacgccggaagaatatgcgtacattaaaaacgatatccagattatcgcagaagctctgctgattcagtttaaacaaggcctggatcgcatgaccgccggcagtgactccctgaaaggtttcaaagatatttccgctataactgatagtgtttcttgcaggccaaccaatgttttagtgcggccttcttatacgcatgtaatttttgctataggtctaatagcgtcttcgagacgactaagtcaaatttgttccggacctagcgtactggcggccgtcactgagggactttccaaagtttctatPmeIStuIK382GD384ID386YH388KE390RP392VG394YK396IT398PE400EY402AY404IK406ND408IQ410II412AE414AL416LI418QF420KQ422GL424DR426MT428AG430SD432SL434KG436FK438D5858-8341GST-DNAPol-6xHis7,0207,0407,0607,0807,1007,1207,1407,160
    tcatcaccacgaaaaaattcaaaaaagtgttcccgaccctgagcctgggtctggataaagaagttcgttatgcataccgcggcggttttacgtggctgaacgaccgtttcaaagaaaaagaaattggcgagggtatggtctttgatgtgaatagtctgtatccggctcagatgtaagtagtggtgcttttttaagttttttcacaagggctgggactcggacccagacctatttcttcaagcaatacgtatggcgccgccaaaatgcaccgacttgctggcaaagtttctttttctttaaccgctcccataccagaaactacacttatcagacataggccgagtctacatNsiISacIII440IT442TK444KF446KK448VF450PT452LS454LG456LD458KE460VR462YA464YR466GG468FT470WL472ND474RF476KE478KE480IG482EG484MV486FD488VN490SL492YP494AQ496MY4985858-8341GST-DNAPol-6xHis7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctcccgcctgctgccgtatggcgaaccgatcgttttcgagggtaaatatgtctgggatgaagactacccgctgcatattcagcacatccgttgtgaatttgaactgaaagaaggctatattccgaccattcaaatcaaacgtagccgcttctataagggtaacgaatacctgaaagagggcggacgacggcataccgcttggctagcaaaagctcccatttatacagaccctacttctgatgggcgacgtataagtcgtgtaggcaacacttaaacttgactttcttccgatataaggctggtaagtttagtttgcatcggcgaagatattcccattgcttatggactttPvuISR500LL502PY504GE506PI508VF510EG512KY514VW516DE518DY520PL522HI524QH526IR528CE530FE532LK534EG536YI538PT540IQ542IK544RS546RF548YK550GN552EY554LK5565858-8341GST-DNAPol-6xHis7,3607,3807,4007,4207,4407,4607,4807,5007,520
    agctctggcggtgaaatcgcagacctgtggctgagtaacgtcgatctggaactgatgaaagaacattacgatctgtacaacgttgaatacatctccggcctgaaatttaaagccaccacgggtctgtttaaagatttcattgacaaatggacctacatcaaaaccacgtctgaagtcgagaccgccactttagcgtctggacaccgactcattgcagctagaccttgactactttcttgtaatgctagacatgttgcaacttatgtagaggccggactttaaatttcggtggtgcccagacaaatttctaaagtaactgtttacctggatgtagttttggtgcagacttcBmgBIBsrGISS558GG560EI562AD564LW566LS568NV570DL572EL574MK576EH578YD580LY582NV584EY586IS588GL590KF592KA594TT596GL598FK600DF602ID604KW606TY608IK610TT612SE6145858-8341GST-DNAPol-6xHis7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gtgcaatcaaacagctggctaaactgatgctgaacagcctgtatggcaaatttgcatctaatccggatgttaccggtaaagtcccgtacctgaaagaaaatggcgctctgggttttcgcctgggcgaagaagaaaccaaagatccggtgtatacgccgatgggtgttttcattaccacgttagtttgtcgaccgatttgactacgacttgtcggacataccgtttaaacgtagattaggcctacaatggccatttcagggcatggactttcttttaccgcgagacccaaaagcggacccgcttcttctttggtttctaggccacatatgcggctacccacaaaagtaatgAgeIBstZ17IGA616IK618QL620AK622LM624LN626SL628YG630KF632AS634NP636DV638TG640KV642PY644LK646EN648GA650LG652FR654LG656EE658ET660KD662PV664YT666PM668GV670FI672T5858-8341GST-DNAPol-6xHis7,7207,7407,7607,7807,8007,8207,8407,860
    cgcgtgggcacgttacaccacgatcacggcagcacaagcgtgctatgaccgcattatctactgtgataccgactcaattcatctgaccggcacggaaatcccggatgtgattaaagatatcgttgacccgaaaaaactgggttattgggcacacgaatcgacctttaaacgtgctgcgcacccgtgcaatgtggtgctagtgccgtcgtgttcgcacgatactggcgtaatagatgacactatggctgagttaagtagactggccgtgcctttagggcctacactaatttctatagcaactgggcttttttgacccaataacccgtgtgcttagctggaaatttgcacgaAleIHincIIA674WA676RY678TT680IT682AA684QA686CY688DR690II692YC694DT696DS698IH700LT702GT704EI706PD708VI710KD712IV714DP716KK718LG720YW722AH724ES726TF728KR730A5858-8341GST-DNAPol-6xHis7,8807,9007,9207,9407,9607,9808,0008,0208,040
    aaatacctgcgccagaaaacgtacatccaagacatctacatgaaagaagtcgatggcaaactggtggaaggttcaccggatgactataccgacattaaattttcggtgaaatgcgccggcatgaccgataaaatcaaaaaagaagtcacgttcgaaaatttcaaagtgggtttcatttatggacgcggtcttttgcatgtaggttctgtagatgtactttcttcagctaccgtttgaccaccttccaagtggcctactgatatggctgtaatttaaaagccactttacgcggccgtactggctattttagttttttcttcagtgcaagcttttaaagtttcacccaaagtBfuAIBspMIK732YL734RQ736KT738YI740QD742IY744MK746EV748DG750KL752VE754GS756PD758DY760TD762IK764FS766VK768CA770GM772TD774KI776KK778EV780TF782EN784FK786VG788F5858-8341GST-DNAPol-6xHis8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gtcgcaaaatgaaaccgaaaccggtgcaagttccgggcggcgttgtcctggtggatgataccttcacgattaaacagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggGCACGACTCATAAGGACGACTGCAAATGGCGCGGAGCCCAAGAATCCGCATTcagcgttttactttggctttggccacgttcaaggcccgccgcaacaggaccacctactatggaagtgctaatttgtcgaacgccggcgtgagctcgtggtggtggtggtggtgactctaggccCGTGCTGAGTATTCCTGCTGACGTTTACCGCGCCTCGGGTTCTTAGGCGTAAAgeIEagINotIPspXIS790RK792MK794PK796PV798QV800PG802GV804VL806VD808DT810FT812IK814QL816AA818AL820EH822HH824HH826H*8285858-8341GST-DNAPol-6xHisMISSING_SeqRBS6xHis8,2408,2608,2808,3008,3208,3408,3608,3808,400
    AAAGATAATGACAAAGCCGAATACGCTCGTTTGGTGAAAAATACCAAAGCCAAGATCGCGCGCACGAAGAAAAAATATGGTGTAGACCTTACCGCGGAAATTGATATTCCTGACCTTGATTCATTTGAAACACGCGCGCAGTTCAATAAGTGGAAGGAACAAGCGTCCTCTTTCATTTCTATTACTGTTTCGGCTTATGCGAGCAAACCACTTTTTATGGTTTCGGTTCTAGCGCGCGTGCTTCTTTTTTATACCACATCTGGAATGGCGCCTTTAACTATAAGGACTGGAACTAAGTAAACTTTGTGCGCGCGTCAAGTTATTCACCTTCCTTGTTCGCAGGAGAAAGTSacIIMI...qTPBP8,4208,4408,4608,4808,5008,5208,5408,560
    CTAACCGTGCCAATATGCGTTACCAGTTCGAGAAAAATGCATACGGGGTGGTGGCTTCGAAAGCCAAAATCGCGGAGATTGAACGTAACACGAAAGAGGTTCAGCGGCTGGTTGATGAGAAGATCAAAGCTATGAAAGACAAGGAATACTATGCAGGCGGTAAGCCGCAAGGTACGATTGGCACGGTTATACGCAATGGTCAAGCTCTTTTTACGTATGCCCCACCACCGAAGCTTTCGGTTTTAGCGCCTCTAACTTGCATTGTGCTTTCTCCAAGTCGCCGACCAACTACTCTTCTAGTTTCGATACTTTCTGTTCCTTATGATACGTCCGCCATTCGGCGTTCCATGNsiIAlwNITPBP8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GATTGAACAACGGATAGCCATGACCAGTCCGGCACACGTTACAGGTATTAACAGGCCCCATGATTTTGACTTTTCGAAAGTGCGAAGCTATAGCCGTTTGCGCACCTTAGAAGAAAGCATGGAAATGCGCACTGATCCGCAGTATTACGAAAAAAAAATGATCCAGTTACAGCTGCTAACTTGTTGCCTATCGGTACTGGTCAGGCCGTGTGCAATGTCCATAATTGTCCGGGGTACTAAAACTGAAAAGCTTTCACGCTTCGATATCGGCAAACGCGTGGAATCTTCTTTCGTACCTTTACGCGTGACTAGGCGTCATAATGCTTTTTTTTTACTAGGTCAATGTCGACFspAITPBP8,7608,7808,8008,8208,8408,8608,8808,9008,920
    AACTTTATTAAGAGCGTTGAGGGCAGTTTCAATTCGTTTGATGCGGCAGATGAACTGATAGAAGAATTGAAAAAAATCCCACCGGATGACTTCTATGAACTGTTTCTCCGCATATCAGAAATCTCCTTTGAGGAATTTGATAGTGAGGGAAACACCGTGGAGAACGTTGAAGGGATTGAAATAATTCTCGCAACTCCCGTCAAAGTTAAGCAAACTACGCCGTCTACTTGACTATCTTCTTAACTTTTTTTAGGGTGGCCTACTGAAGATACTTGACAAAGAGGCGTATAGTCTTTAGAGGAAACTCCTTAAACTATCACTCCCTTTGTGGCACCTCTTGCAACTTCCCTAloITPBP8,9408,9608,9809,0009,0209,0409,0609,0809,100
    ACGTATATAAAATCCTGAGCTACCTGGAACAGTACCGTCGCGGCGACTTTGATTTAAGCCTGAAAGGCTTCTAACTGACTCGAGACAGGGAGGAACCATATGGCAAAAATGATGCAGCGGGAAATTACCAAGACTACCGTGAACGTTGCCAAAATGGTGATGGTGGATGGGGAGGTGCATATATTTTAGGACTCGATGGACCTTGTCATGGCAGCGCCGCTGAAACTAAATTCGGACTTTCCGAAGATTGACTGAGCTCTGTCCCTCCTTGGTATACCGTTTTTACTACGTCGCCCTTTAATGGTTCTGATGGCACTTGCAACGGTTTTACCACTACCACCTACCCCTCCNdeIRBSTPBPDSBP69,1209,1409,1609,1809,2009,2209,2409,260
    TTCAGGTAGAGCAATTACCGAGTGAAACATTTGTGGGTAATCTGACGATGGAACAGGCGCAATGGCGCATGAAGCGCAAATATAAAGGCGAACCTGTTCAAGTGGTCAGCGTCGAAAGTCCATCTCGTTAATGGCTCACTTTGTAAACACCCATTAGACTGCTACCTTGTCCGCGTTACCGCGTACTTCGCGTTTATATTTCCGCTTGGACAAGTTCACCAGTCGCAGCTTPBPDSBP69,2809,3009,3209,3409,3609,380
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Assembly Parameters
    • Linear Map
    Editing disabled on read-only sequence.
    9390 bp70007500800085009000500100015002000GST-DNAPol-6xHisoriAmp-RRBSTPBP5858-8341pGS-21a his6-GST-WTPhi29DNAP FWD (DNA)F1ORI_2_REV (DNA)

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create Primers
    Attach Existing
    Detach All Primers
    Primer Position Product Size

    History

    Lineage

     Show Full History
    Change User Timestamp

    Sequence Alignments

    Create New Alignment
    Saved Alignments

    Sequence Info

    Tags

    Name
    Parent Version
    Folder
    Topology
    Start Index
    Locked
    Created
    --
    Length
    --
    Export Data
    Download
    Split Workspace
    Bases
    9390