Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pAAV-nEF-ConFoff 2.0-hChR2(E123T-T159C)-EYFP-WPRE
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtaaggatctgcgatcgctccgggacgtccgtcgacgcgcgagcgagcgagtgactccggcgggcccgtttcgggcccgcagcccgctggaaaccagcgggccggagtcactcgctcgctcgcgcgtctctccctcaccggttgaggtagtgatccccaaggacgccggcgtgcgcattcctagacgctagcgaggcMluIPvuISbfIAsiSIAflIIIL-ITREF1 promoter20406080100120140160
    gtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaacgggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtcacgggcagtcacccgtctcgcgtgtagcgggtgtcaggggctcttcaacccccctccccagccgttaacttgcccacggatctcttccaccgcgccccatttgaccctttcactacagcacatgaccgaggcggaaaaagggctcccaccccctcttggcatatattcacgtcaMfeIEcoNIEF1 promoter180200220240260280300320340
    agtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacagctgaagcttcgaggggctcgcatctctccttcacgcgcccgccgccctacctgaggccgccatccacgccggttgagtcgcgttctgccgcctcccgcctgtggtgcctcctgaactgcgtccgcctcagcggcacttgcaagaaaaagcgttgcccaaacggcggtcttgtgtcgacttcgaagctccccgagcgtagagaggaagtgcgcgggcggcgggatggactccggcggtaggtgcggccaactcagcgcaagacggcggagggcggacaccacggaggacttgacgcaggcggCspCIEcoNIBsu36IHindIIIEF1 promoter360380400420440460480500520
    gtctaggtaagtttaaagctcaggtcgagaccgggcctttgtccggcgctcccttggagcctacctagactcagccggctctccacgctttgcctgaccctgcttgctcaactctacgtctttgtttcgttttctgttctgcgccgttacagatccaagctgtgaccggcgcctacagatccattcaaatttcgagtccagctctggcccggaaacaggccgcgagggaacctcggatggatctgagtcggccgagaggtgcgaaacggactgggacgaacgagttgagatgcagaaacaaagcaaaagacaagacgcggcaatgtctaggttcgacactggccgcggatKasINarISfoIPluTIEF1 promoter540560580600620640660680700
    cggatccgaagttcctattctctagaaagtataggaacttcgcagagtagtagctggattgtagctgctattagcaatctaaaacctcttagaagttcctattcttcaaaaggtataggaacttcggcgcgccgccaccatggactatggcggcgctttgtctgccgtcggacgcgcctaggcttcaaggataagagatctttcatatccttgaagcgtctcatcatcgacctaacatcgacgataatcgttagattttggagaatcttcaaggataagaagttttccatatccttgaagccgcgcggcggtggtacctgataccgccgcgaaacagacggcagcctgcgAscIXbaIBamHIChR2 ET/TC Exon 1FRTF5720740760780800820840860
    gaacttttgttcgttactaatcctgtggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccggatggattgaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagcaggattcagcattttgctgctgatgttctatgcttgaaaacaagcaatgattaggacaccaccacttgcccaggcaggaccagggactcctagttacaatgacacggcctacctaacttagagcgccgtgcttgccgcgagtctggcgcagtttacaggacgtcaccgaacgtcgtcctaagtcgtaaaacgacgactacaagatacPflFIBsu36ITth111IChR2 ET/TC Exon 18809009209409609801,0001,0201,040
    cctaccaaacctggaaatctacatgcggctgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttcttttttgagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgctatgcaacctggctgctcacttgtccggatggtttggacctttagatgtacgccgaccctcctctagatacacacgcggtaactttaccaattccactaagagctcaagaaaaaactcaaattcttagggagatacgagatggaacggtgtcctgtggcccacgtcaccgacgcgatacgttggaccgacgagtgaacaggTliIXhoIAlwNIBglIIBstAPIDraIIIPaeR7IChR2 ET/TC Exon 11,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgtcatccttatccacctgagcaacctcaccggtaagtgtcggggttgagctcataacttcgtatagtataccttatacgaagttattctttgcaccattctaaagaataacagtgataatttctgggttaaggcaaataacttcgtataggatactttatacgaagttatggtaacagtaggaataggtggactcgttggagtggccattcacagccccaactcgagtattgaagcatatcatatggaatatgcttcaataagaaacgtggtaagatttcttattgtcactattaaagacccaattccgtttattgaagcatatcctatgaaatatgcttcaataccatAgeISacIXmnIAcc65IBstZ17IEco53kIlox Nlox 2722ChR2 ET/TC Exon 1CMV ...nor1,2401,2601,2801,3001,3201,3401,3601,3801,400
    cccctcctgtactcaccttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcaccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggggaggacatgagtggaacggccaccacgtctacttgaagtcccagtcgaacggcatccaccgtagcgggagcgggagcggcctgtgcgacttgaacaccggcaaatgcagtggcaggtcgagctggtcctacccgtggtggggccacttgtcgaggagcgggaacgagtggtaKpnIEYFP Exon 2CE i...nor1,4201,4401,4601,4801,5001,5201,5401,560
    ggtggcggccgctggcacggctccggcctcggcttcgtcttcgacgagagtctcgacctcgatctccgttccgccgatgttcagtttggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttaccaccgccggcgaccgtgccgaggccggagccgaagcagaagctgctctcagagctggagctagaggcaaggcggctacaagtcaaaccaccaaaacgcctatagaggcacttagttatacacgagcacctagtcctgcgcgtccatcacagggttgttgggggttgttaaaaatBfuAIBspMIEcoRVPshAIChR2 ET/TC Exon 2spacer1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctcatcagatcaataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattgggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagtatgataaccctgagtagtctagttattagcacactggatgccacctcggtatctgcgagtcctgcggttttggaagcccggggttttacttctcttaacccttgtatggggtcgagtgctttttgtcggtacggtacggccagtgctggaccgccgtggctgggaaaccgtgtcatactattgggaApaIPspOMIChR2 ET/TC Exon 21,7601,7801,8001,8201,8401,8601,8801,9001,920
    cgatatatgctttggcggcgtgaaaaaatgtgttcgcgccatagcacaatccaagacaaaagaagatgactttaacatagccggttgccatggcgctggtagccccccacacgatacacccgatgtctgagacaaggagtcccatggttctcctgctgtagtcgttgctcaggccgctatatacgaaaccgccgcacttttttacacaagcgcggtatcgtgttaggttctgttttcttctactgaaattgtatcggccaacggtaccgcgaccatcggggggtgtgctatgtgggctacagactctgttcctcagggtaccaagaggacgacatcagcaacgagtccggStuIChR2 ET/TC Exon 21,9401,9601,9802,0002,0202,0402,0602,0802,100
    taaacacatgagaaatagagtcataagcacatgggtcacatacaggagatatgtatataacattaatacaattttataaaaaaaagggggggcacaactagtataacttcgtataaggtatactatacgaagttattaagaggtttcatattgctaatagcagctacaatccagcatttgtgtactctttatctcagtattcgtgtacccagtgtatgtcctctatacatatattgtaattatgttaaaatattttttttcccccccgtgttgatcatattgaagcatattccatatgatatgcttcaataattctccaaagtataacgattatcgtcgatgttaggtcgAseIPsiISpeIBstZ17ICMV bplox N RevCMV ...tor2,1202,1402,1602,1802,2002,2202,2402,260
    taccattctgcataacttcgtataaagtatcctatacgaagttatgtcgactggagagtggccagcccttctcatgttcagagaacatggttaactggttaagtcatgtcgtcccacagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttatggtaagacgtattgaagcatatttcataggatatgcttcaatacagctgacctctcaccggtcgggaagagtacaagtctcttgtaccaattgaccaattcagtacagcagggtgtcgacgggcacgggaccgggtgggagcactggtggaagccgatgccggacgtcacgaaHpaISalIHincIIHincIICE bpEYFP Exon 3lox 2722 Rev2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacgcgggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaagaagttcctgctgccgttgatgttctgggcgcggctccacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtagctgEYFP Exon 32,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccaaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgatgttgtcggtgttgcagatatagtaccggctgttcgtcttcttgccgtagttccacttgaagttctaggcggtgttgtagctcctgccgtcgcacgtcgagcggctggtgatggtcgtcttgtgggggtEYFP Exon 32,6402,6602,6802,7002,7202,7402,7602,7802,800
    tcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaagctagcgaagttcctatactagccgctgccggggcacgacgacgggctgttggtgatggactcgatggtcaggcgggactcgtttctggggttgctcttcgcgctagtgtaccaggacgacctcaagcactggcggcggccctagtgagagccgtacctgctcgacatgttcattcgatcgcttcaaggatatgaBmtINheIBsrGIEYFP Exon 3FRT rev2,8202,8402,8602,8802,9002,9202,9402,960
    ttctagagaataggaacttctaagcagacaatcttgtaatacatcagcagctagtagattgctccagctagaagttcctataccttttgaagaataggaacttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattctaagatctcttatccttgaagattcgtctgttagaacattatgtagtcgtcgatcatctaacgaggtcgatcttcaaggatatggaaaacttcttatccttgaagcttaagctatagttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaClaIXbaIBspDIBstBIEcoRIEcoRVHindIIIWPREF5 revFRT rev2,9803,0003,0203,0403,0603,0803,1003,1203,140
    taactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagagaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaaWPRE3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacgacctgtccccgagccgacaacccgtgactgttaaggcaccacaacagcccctttaPflMIWPRE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    catcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctgtagcaggaaaggaaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagacgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaZraIAatIISacIIWPRE3,5203,5403,5603,5803,6003,6203,6403,660
    ttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataaacccggcggaggggcgtagctatggctcgcgacgagctctctagatgcccaccgtagggacactggggaggggtcacggagaggaccgggaccttcaacggtgaggtcacgggtggtcggaacaggattattttaattcaacgtagtaaaacagactgatccacaggaagatatAfeIBplIBplIClaITliIXhoIBglIIBspDICspCIPaeR7IhGH polyAWPRE3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgtttataataccccacctccccccaccatacctcgttccccgttcaacccttctgttggacatcccggacgccccagataacccttggttcgacctcacgtcaccgtgttagaaccgagtgacgttagaggcggaggacccaagttcgctaagaggacggagtcggagggctcaacaaBbvCIhGH polyA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttccctaaggtccgtacgtactggtccgagtcgattaaaaacaaaaaaaccatctctgccccaaagtggtataaccggtccgaccagaggttgaggattagagtccactagatgggtggaaccggagggtttaacgaccctaatgtccgcacttggtgacgagggaagggacaggaaBlpINsiISphIBsaXIhGH polyA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgactaaaacatccattggtgcacgcctggctcgccggcgtccttggggatcactacctcaaccggtgagggagagacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggaPmlIBsaAIRsrIIBstEIIR-ITR4,2204,2404,2604,2804,3004,3204,3404,360
    gcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgcgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcKasINarISbfISfoIPluTIf1 Origin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaaBsaAIDraIIIf1 Origin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgctgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcPsiIBsaXIf1 Origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    tttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcacaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtg4,9204,9404,9604,9805,0005,0205,0405,060
    cgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactcZraIAatII5,0805,1005,1205,1405,1605,1805,2005,2205,240
    acaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaAmpR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaacXmnIAmpR5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaPvuIScaIAmpR5,6205,6405,6605,6805,7005,7205,7405,760
    tgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaaAseIFspIAmpR5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctggacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgacAmpR5,9605,9806,0006,0206,0406,0606,0806,1006,120
    agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagatctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctAmpR6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacagpUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    cttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccAlwNIpUC ori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    ggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcgpUC ori6,6606,6806,7006,7206,7406,7606,7806,8006,820
    ctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtgaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIpUC ori6,8406,8606,8806,9006,9206,9406,960
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    6977 bp500055006000650050010001500SNP

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    6977