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ESP1xS-opt3: S-AGGG NU....NN lib
  • Sequence Map
    Editing disabled on read-only sequence.
    tgtgcttctcaaatgcctgaggccagtttgctcaggctctccccgtggaggtaataattgacgatatgatcagtgcacggctaactaagcggcctgctgactttctcgccgatcaaaaggcattttgctattaagggattgacgagggcgtatctgcgcagtaagatagcagacaacacgaagagtttacggactccggtcaaacgagtccgagaggggcacctccattattaactgctatactagtcacgtgccgattgattcgccggacgactgaaagagcggctagttttccgtaaaacgataattccctaactgctcccgcatagacgcgtcattctatcgtctgtBclIBtgIFspIApaLIBaeGIBsu36IProK promoterStandard phage backbone20406080100120140160
    agatgggtcccttatcatggcaaccatctgaacggagagatgccggagcggctgaacggaccggtNtccctNNaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactttatcaatgacttatctcaggatccgtcgacctgcagatccttagcgaatctacccagggaatagtaccgttggtagacttgcctctctacggcctcgccgacttgcctggccaNagggaNNtggcctcatccccgttgagatggcccccaagtttagggggagagaggcggtgaaatagttactgaatagagtcctaggcagctggacgtctaggaatcgcttAccIAgeIBglIEciIKflIPstISalIAvaIIAvaIIBamHIBfuAIBspMIPpuMIRsrIIHincIIS-WTtRNA Serrrn...or180200220240260280300320340
    agctaaggattttttttaagcttctgccattcatccgagaaggagattttcaacatgctccctcaatcggttgaatgtcgcccttttgtctttggcgctggtaaaccatatgaattttctattgattgtgacagaataaacttattccgtggtgtctttgcgtttcttttatatgtcgattcctaaaaaaaattcgaagacggtaagtaggctcttcctctaaaagttgtacgagggagttagccaacttacagcgggaaaacagaaaccgcgaccatttggtatacttaaaagataactaacactgtcttatttgaataaggcaccacagaaacgcaaagaaaatatacBtgINdeIHindIIIIIIA0.44 (gVI)rrnC terminatorUSER JunctionStandard phage backbone360380400420440460480500520
    ttgccacctttatgtatgtatcttctacgtttgctaacatactgcgtaataaggagtcttaatcatgccagttcttttgggtattccgttattattgcgtttcctcggtttccttctggtaactttgttcggctatctgcttacttttctcaaaaagggcttcggtaagatagctaacggtggaaatacatacatagaagatgcaaacgattgtatgacgcattattcctcagaattagtacggtcaagaaaacccataaggcaataataacgcaaaggagccaaaggaagaccattgaaacaagccgatagacgaatgaaaagagtttttcccgaagccattctatcgaMslIVIIIIStandard phage backbone540560580600620640660680700
    attgctatttcattgtttcttgctcttattattgggcttaactcaattcttgtgggttatctctctgatattagtgctcaattaccctttgactttgttcagggtgttcagttaattctcccgtctaatgcgcttccctgtttttatgttattctctctgtaaagactgctattttaacgataaagtaacaaagaacgagaataataacccgaattgagttaagaacacccaatagagagactataatcacgagttaatgggaaactgaaacaagtcccacaagtcaattaagagggcagattacgcgaagggacaaaaatacaataagagagacatttctgacgataaaCspCIVIA0.49 (gI)Standard phage backbone720740760780800820840860
    tcatttttgacgttaaacaaaaaatcgtttcttatttggattgggacaaataatatggctgtttattttgtaactggcaaattaggctctggaaagacgctcgttagcgttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaatcttgatttaaggcttcagtaaaaactgcaatttgttttttagcaaagaataaacctaaccctgtttattataccgacaaataaaacattgaccgtttaatccgagacctttctgcgagcaatcgcaaccattctaagtcctattttaacatcgacccacgttttatcgttgattagaactaaattccgaagHgaIBseYIIVIStandard phage backbone8809009209409609801,0001,0201,040
    aaaacctcccgcaagtcgggaggttcgctaaaacacctcgcgttcttagaataccggataagccttctatatctgatttgcttgctattgggcgcggtaatgattcctacgatgaaaataaaaacggattgcttgttctcgatgagtgcggtacttggtttaatacccgttcttgttttggagggcgttcagccctccaagcgattttgtggagcgcaagaatcttatggcctattcggaagatatagactaaacgaacgataacccgcgccattactaaggatgctacttttatttttgcctaacgaacaagagctactcacgccatgaaccaaattatgggcaagaacAloIIStandard phage backbone1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gaatgataaggaaagacagccgattattgattggtttttacatgctcgtaaattaggatgggatattatttttcttgttcaggacttatccattgttgataaacaggcgcgttctgcattagctgaacatgttgtttattgtcgtcgtctggacagaattactttaccttttgtccttactattcctttctgtcggctaataactaaccaaaaatgtacgagcatttaatcctaccctataataaaaagaacaagtcctgaataggtaacaactatttgtccgcgcaagacgtaatcgacttgtacaacaaataacagcagcagacctgtcttaatgaaatggaaaacagIStandard phage backbone1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ggtactttatattctcttattactggctcgaaaatgcctctgcctaaattacatgttggcgttgttaaatatggcgattctcaattaagccccactgttgagcgttggctttatactggtaagaatttgtataacgcatacgatactaaacaggctttttctagtaattatgattccatgaaatataagagaataatgaccgagcttttacggagacggatttaatgtacaaccgcaacaatttataccgctaagagttaattcggggtgacaactcgcaaccgaaatatgaccattcttaaacatattgcgtatgctatgatttgtccgaaaaagatcattaatactaaIStandard phage backbone1,4201,4401,4601,4801,5001,5201,5401,560
    ccggtgtttattcttatttaacgccttatttatcacacggtcggtatttcaaaccattaaatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttctcgcgttctttgtcttgcgattggatttgcatcagcatttacatatagttatataacccaacctaaggccacaaataagaataaattgcggaataaatagtgtgccagccataaagtttggtaatttaaatccagtcttctactttaattgattttatataaactttttcaaaagagcgcaagaaacagaacgctaacctaaacgtagtcgtaaatgtatatcaatatattgggttggattBsaBIIgXIStandard phage backbone1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gccggaggttaaaaaggtagtctctcagacctatgattttgataaattcactattgactcttctcatcgtcttaatctaagctatcgctatgttttcaaggattctaagggaaaattaattaatagcgacgatttacagaagcaaggttattcacttacatatattgatttatgtcggcctccaatttttccatcagagagtctggatactaaaactatttaagtgataactgagaagagtagcagaattagattcgatagcgatacaaaagttcctaagattcccttttaattaattatcgctgctaaatgtcttcgttccaataagtgaatgtatataactaaatacaEarIPacIBcoDIBsmAIIgXIA0.64 (gIV)Standard phage backbone1,7601,7801,8001,8201,8401,8601,8801,9001,920
    actgtttccattaaaaaaggtaattcaaatgaaattgttaaatgtaattaattctgttttcttgatgtttgtttcatcatcttcttttgctcaggtaattgaaatgaataattcgcctctgcgcgattttataacttggtattcaaagcaatcaggcgaatccgttattgtttcttgacaaaggtaattttttccattaagtttactttaacaatttacattaattaagacaaaagaactacaaacaaagtagtagaagaaaacgagtccattaactttacttattaagcggagacgcgctaaaatattgaaccataagtttcgttagtccgcttaggcaataacaaagaIVIgXIStandard phage backbone1,9401,9601,9802,0002,0202,0402,0602,0802,100
    cccgatgtaaaaggtactgttactgtatattcatctgacgttaaacctgaaaatctacgcaatttctttatttctgttttacgtgcaaataattttgatatggtaggttctaacccttccattattcagaagtataatccaaacaatcaggattatattgatgaattgccatcacgggctacattttccatgacaatgacatataagtagactgcaatttggacttttagatgcgttaaagaaataaagacaaaatgcacgtttattaaaactataccatccaagattgggaaggtaataagtcttcatattaggtttgttagtcctaatataactacttaacggtagtgIVStandard phage backbone2,1202,1402,1602,1802,2002,2202,2402,260
    ctgataatcaggaatatgatgataattccgctccttctggtggtttctttgtcccgcaaaatgataatgttactcaaacttttaaaattaataacgttcgggcaaaggatttaatacgagttgtcgaattgtttgtaaagtctaatacttctaaatcctcaaatgtattatctatgactattagtccttatactactattaaggcgaggaagaccaccaaagaaacagggcgttttactattacaatgagtttgaaaattttaattattgcaagcccgtttcctaaattatgctcaacagcttaacaaacatttcagattatgaagatttaggagtttacataatagataAclIIVStandard phage backbone2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tgacggatctaatctattagttgttagtgctcctaaagatattttagataacctttctcaattcctttcaattgttgatttgccaactgaccaggtattgattgagggtttgatatttgaggttcagcaaggtgatgctttagatttttcatttgctgctggctctcagcgtggcactgcctagattagataatcaacaatcacgaggatttctataaaatctattggaaagagttaaggaaagttaacaactaaacggttgactggtccataactaactcccaaactataaactccaagtcgttccactacgaaatctaaaaagtaaacgacgaccgagagtcgcaccgMfeIBstNIPspGISexAIIVStandard phage backbone2,4602,4802,5002,5202,5402,5602,5802,6002,620
    actgttgcaggcggagttaatactgaccgcctcacctctgttctatcttctgctggtggttcgttcggtatttttaatggcgatgttttagggctatcagttcgcgcattaaagactaatagccagtcaaaaatattgtctgtgccacgtattcttacgctttcaggtcagaaggtgacaacgtccgcctcaattatgactggcggagtggagacaagatagaagacgaccaccaagcaagccataaaaattaccgctacaaaatcccgatagtcaagcgcgtaatttctgattatcggtcagtttttataacagacacggtgcataagaatgcgaaagtccagtcttccEciIIVStandard phage backbone2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gttctatctctattggccagaatgtcccttttattactggtcgtgtaactggtgaatctgccaatgtaaataatccatttcagacgattgagcgtcaaaatgtaggtatttccatgagcgtttttcctgttgcaatggctggcggtaatattgttctggatattaccagcaaggccaagatagagataaccggtcttacagggaaaataatgaccagcacattgaccacttagacggttacatttattaggtaaagtctgctaactcgcagttttacatccataaaggtactcgcaaaaaggacaacgttaccgaccgccattataacaagacctataatggtcgttccgHgaIMscIIVStandard phage backbone2,8202,8402,8602,8802,9002,9202,9402,960
    cgatagtttgagttcttctactcaggcaagtgatgttattactaaccaaagaagtattgctacaacggttaatttgcgtgatggacagactcttctactcggtggcctcactgattataaaaacacttctcaggattctggcgtaccgttcctgtctaaaatccctttaatcggcgctatcaaactcaagaagatgagtccgttcactacaataatgattggtttcttcataacgatgttgccaattaaacgcactacctgtctgagaagatgagccaccggagtgactaatatttttgtgaagagtcctaagaccgcatggcaaggacagattttagggaaattagccgBarIEarIIVStandard phage backbone2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ctcctgtttagctcccgctctgattctaacgaggagagcacgttatacgtgctcgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcgaggacaaatcgagggcgagactaagattgctcctctcgtgcaatatgcacgagcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagBsaXIBseRIIVpackaging signalStandard phage backbone3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ccttcctttctcgccacgttcgccggctttccccgtcaagcgctaaatcgggggcccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggaggaaggaaagagcggtgcaagcggccgaaaggggcagttcgcgatttagcccccggggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctAfeIApaIBanIDrdIMmeINaeIBaeGIBanIIDraIIINgoMIVPspOMIRNA pri...cationStandard phage backbone3,3403,3603,3803,4003,4203,4403,4603,4803,500
    gtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccggtttcggcctattggttaaaaaatgaactgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggccaaagccggataaccaattttttacttgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaAclIAloIAvaIBsaXIBsoBIcomplete (+) originessential (+) oriG0.92 (gII)Standard phage backbone3,5203,5403,5603,5803,6003,6203,6403,660
    taaatatttgcttatacaatcttcctgtcttgggggcttttcttattatcaaccggggtacatatgattgacatgctagttttacggttaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagatctctcaaaaatagctaccctctatttataaacgaatatgttagaaggacagaacccccgaaaagaataatagttggccccatgtatactaactgtacgatcaaaatgccaatggcaagtagctaagagaacaaacgaggtctgagagtccgttactggactatcggaaacatctagagagtttttatcgatgggagaBpmIClaINdeISwaIBglIIBspDIBstEIIIIG0.92 (gII)gI...SStandard phage backbone3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ccggcatgaatttatcagctagaacggttgaatatcatgttgatggtgatttgactgtctccggcctttaccacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcggccgtacttaaatagtcgatcttgccaacttatagtacaactaccactaaactgacagaggccggaaatggtgggaaaacttagaaatggatgtgtaatgagtccgtaacgtaaattttatatactcccaagatttttaaaaataggaacgcaactttatttccgaagagggcgMslIBcoDIBsmAIIIG0.99 (gII')Standard phage backbone3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaaagtattgcaggggcataatgtttttggtacaaccgatttaggtttatgttctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaacgctactactattagtagaattgatgccgccttttcagcccgcgccccaaatgaaaatttttcataacgtccccgtattacaaaaaccatgttggctaaatccaaatacaagactccgaaataacgaattaaaacgattaagaaacggaacggacatactaaataacctacaattgcgatgatgataatcatcttaactacggcggaaaagtcgggcgcggggtttacttttaHpaIHincIIIIStandard phage backbone4,0404,0604,0804,1004,1204,1404,1604,1804,200
    atagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctatatcgatttgtccaataactggtaaacgctttacatagattaccagtttgatttagatgagcaagcgtcttaacccttagttgacaatgtaccttactttgaaggtctgtggcatgaaatcaacgtataaattttgtacaactcgatgtcgtggtctaagtcgttaattcgagatIIStandard phage backbone4,2204,2404,2604,2804,3004,3204,3404,360
    agccatccgcaaaaatgacctcttatcaaaaggaacaattaaggatactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgagatacttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgatcggtaggcgtttttactggagaatagttttccttgttaattcctatgagagattaggactggacaacctcaaacgaaggccagaccaagcgaaacttcgagcttaattttgctctatgaacttcagaaagcccgaaggagaattagaaaaactacgttaggcgaaacgaagactMmeIXmnIBciVIIIG0.06 (gX)Standard phage backbone4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ctataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgaagggaattcaatgaatatttatgacgattccgcagtattggccgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagatattatcagtcccatttctggactaaaaactaaataccagtaagagcaaaagacttgacaaatttcgtaaacttcccttaagttacttataaatactgctaaggcgtcataaccggcgataggtcagatttgtaaaatgataatgggggagaccgttttgaagaaaacgttttEcoRIIIXG0.06 (gX)gX RBSStandard phage backbone4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcctctcgctattttggtttctatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttccggagagcgataaaaccaaagatagcagcagaccatttgctcccaatactatcacaacgagaatgatacggagcattaaggaaaaccgcaatacatagacgtaatcaacttacaccataaggatttagagttgactacttagaaagatggacattattacaacaaggcaatcaagIIXStandard phage backbone4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgtttctcgtcagggcaagccttattcactgaatgagcagcaaaataattgcatctaaaaagaagggttgcaggactgaccatattactcggtcaagaattttagcgtattccattaagtgttactaatttcaactttaatttggtagagttcgggttaaatgatgagcaagaccacaaagagcagtcccgttcggaataagtgacttactcgtcSmlIBpuEIVIIXG0.12 (gV)gV RBSStandard phage backbone4,9204,9404,9604,9805,0005,0205,0405,060
    ttttgttacgttgatttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtcagccagcctatgcgccgggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatgaaaacaatgcaactaaacccattacttataggccaagaacagttctaatgagaactacttccagtcggtcggatacgcggcccagacatgtggcaagtagacaggagaaagtttcaaccagtcaagccaagggaatactaactggcagacgcggagcaaggccgattcattgtacBfuAIBspMIBsrGIVStandard phage backbone5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gagcaggtcgcggatttcgacataatttatcaggcgatgatacaaatctccgttgtactctgtttcgcgcttggtataatagctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgcctccgtagtggcattacgtattttacccgtttctcgtccagcgcctaaagctgtattaaatagtccgctactatgtttagaggcaacatgagacaaagcgcgaaccatattatcgacccccagtttctactcacaaaatcacataagaaagcggagaaagcaaaatccaaccacggaggcatcaccgtaatgcataaaatgggcaaaBanIBsaBIBseYISnaBIVIIIXG0.18 (gVIII)Standard phage backbone5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttgactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgttacctttgaaggagtactttttcagaaatcaggagtttcggagacatcggcaacgatgggagcaaggctacgacagaaagcgacgactcccactgctagggcgttttcgccggaaactgagggacgttcggagtcgctggcttatatagccaatacgcacccgctaccaacaacBbvCIBbvCIBspHIVIIIIXgVI...BSStandard phage backbone5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagttgataaactgatacaattaaaggctccttttggagcctttttttttgatgcggccgcgatagtaacagccgcgttgatagccatagttcgacaaattctttaagtggagctttcgttcaactatttgactatgttaatttccgaggaaaacctcggaaaaaaaaactacgccggcgctaEagINotIVIIIX0.25 (gIII)terminatorStandard phage backboneUSE...on5,6205,6405,6605,6805,700
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    Editing disabled on read-only sequence.
    5719 bp42504500475050005250550025050075010001250IIIPACE variantXVIanticodonStandard phage backboneED1405 (DNA)

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    5719