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JPZF6 CMV-TO-SCN1A-114-NZF
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMV20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMV180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMV360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactCTAGCGTTTAAACTTAAGCTTCGCCACCATGTATCAGTGTGGTCAGTGCGGTAAATCCTTCAGGCAATCTAGTAACTTACATCAACACCATAGGTTACATCATACCGGGGAGAGGCCCTTTATCTGTGAGGTGTGCCACAAGTCttctgtggccctggctaggtcggaggcctgaGATCGCAAATTTGAATTCGAAGCGGTGGTACATAGTCACACCAGTCACGCCATTTAGGAAGTCCGTTAGATCATTGAATGTAGTTGTGGTATCCAATGTAGTATGGCCCCTCTCCGGGAAATAGACACTCCACACGGTGTTCAGBbsIPmeIAflIIHindIIIMY2QC4GQ6CG8KS10FR12QS14SN16LH18QH20HR22LH24HT26GE28RP30FI32CE34VC36HK38S760-1932720740760780800820840860
    CTACACTCAATTTTCCAACCTGTGTCGGCATAAAAGGATGCACGCGGATTGCACCGGAGAAAGGCCATATCAGTGCGGACAGTGTGGTAAATCTTTCCGTCAAAGCTCAAACCTGCACCAACATCATAGGCTGCATCACACGGGCGAGCGGCCGCATGAGTGTGCAAGGTGTGGTGATGTGAGTTAAAAGGTTGGACACAGCCGTATTTTCCTACGTGCGCCTAACGTGGCCTCTTTCCGGTATAGTCACGCCTGTCACACCATTTAGAAAGGCAGTTTCGAGTTTGGACGTGGTTGTAGTATCCGACGTAGTGTGCCCGCTCGCCGGCGTACTCACACGTTCCACACCABsgINotIBfuAIBspMIY40TQ42FS44NL46CR48HK50RM52HA54DC56TG58ER60PY62QC64GQ66CG68KS70FR72QS74SN76LH78QH80HR82LH84HT86GE88RP90HE92CA94RC96G760-19328809009209409609801,0001,0201,040
    AAGAACTTTTCTTGGCATAGTGACCTCATACTGCATGAACAGATTCATACAGGGGAACGCCCGTTTGTTTGCGGCGTATGTAAAATGGGGTTTAGCCTGTTAACTTCATTGGCTCAACATCACAGTAGTCACACCGGCGAAAGGCCGTACACTTGCGAGATATGTAACAAATGTTTTCTTGAAAAGAACCGTATCACTGGAGTATGACGTACTTGTCTAAGTATGTCCCCTTGCGGGCAAACAAACGCCGCATACATTTTACCCCAAATCGGACAATTGAAGTAACCGAGTTGTAGTGTCATCAGTGTGGCCGCTTTCCGGCATGTGAACGCTCTATACATTGTTTACAAHpaIXmnISgrAIK98NF100SW102HS104DL106IL108HE110QI112HT114GE116RP118FV120CG122VC124KM126GF128SL130LT132SL134AQ136HH138SS140HT142GE144RP146YT148CE150IC152NK154C760-19321,0601,0801,1001,1201,1401,1601,1801,2001,220
    TTACGAGGTCTGCCGTGTTGCGACGTCATAAGAAGATGCATTGCACCGGAGAGCGTCCTTTTGAATGTAGTAAATGCGGGAAAGCATGCACGAGAAGATGCAACCTGATTCAACACCAAAAGGTCCATACTGGTGAGAGGCCATTCCAATGTGCACTGTGTCAAAAGTCATTTACAATGCTCCAGACGGCACAACGCTGCAGTATTCTTCTACGTAACGTGGCCTCTCGCAGGAAAACTTACATCATTTACGCCCTTTCGTACGTGCTCTTCTACGTTGGACTAAGTTGTGGTTTTCCAGGTATGACCACTCTCCGGTAAGGTTACACGTGACACAGTTTTCAGTAAATGNsiISphIF156TR158SA160VL162RR164HK166KM168HC170TG172ER174PF176EC178SK180CG182KA184CT186RR188CN190LI192QH194QK196VH198TG200ER202PF204QC206AL208CQ210KS212FT214760-19321,2401,2601,2801,3001,3201,3401,3601,3801,400
    TCAACTTGCTCATTTGCAGAAACACCATTTGGTACACGCTGCTGCAGGCGGAAGCGGAGGCTCTGGCGGATCtGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCAGTTGAACGAGTAAACGTCTTTGTGGTAAACCATGTGCGACGACGTCCGCCTTCGCCTCCGAGACCGCCTAGaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGAjuIBlpIBsgIPasIPstIBstXIQL216AH218LQ220KH222HL224VH226AA228AG230GS232GG234SG236GS238EG240QS242DE244RA246LL248DQ250LH252TL254LS256NT258DA260TG262LE264EI266DR268AL270GI272760-19321,4201,4401,4601,4801,5001,5201,5401,560
    CCCGAGCTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGGGCTCGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGAAvaIPasIBmgBIBsoBIEcoNIEcoRIPflMIPE274LV276NQ278GQ280AL282EP284KQ286DG288GW290GS292MA294EE296FV298TL300KD302VG304MD306FT308LG310DW312EQ314LG316LE318QG320DT322FW324DT326AL328DN330760-19321,5801,6001,6201,6401,6601,6801,7001,7201,740
    GCCAGGACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACGGTCCTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTPciIBstXIPpuMICQ332DL334FL336LD338PP340GG342GD344GL346GL348GH350EK352FP354SD356LD358LD360MF362NG364SL366EC368DM370ES372II374RS376EL378MD380AD382GL384DF386NF388D760-19321,7601,7801,8001,8201,8401,8601,8801,9001,920
    CAGCtaggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccGTCGatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactggApaIAvaIBclIPmeITliIXhoIBsoBIPspXIPaeR7IPspOMIS390*BGH polyadenylation sequencec-myc epitopePolyhi...e tag1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagBbsIClaISphIBspDIBGH polyadenylation sequence2,1202,1402,1602,1802,2002,2202,2402,260
    gatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaaBGH pA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtgaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcaNaeIXbaINgoMIVFRT2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgtatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcBarIMscIBsaXIPflFIPshAITth111IHygromycinFRT2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagaBfuAIBspMIEcoRIDraIIIHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    gcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtccgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagNcoINdeIPstIPvuIAsiSIPflFIRsrIITth111IHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    agtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccDraIIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    tcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgAjuISacIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactaaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatScaIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    cgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaattgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaNaeIPfoIPsiINgoMIVSV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagcagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcgBsmIBstZ17ISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcSapIBsaXIBspQI4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatPciIpUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    ggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcapUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    ggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgapUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaacgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaattttBspHIpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    atgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcaAhdIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgtBsaIFspIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    acgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaaAclIPvuIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    atcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaBcgIScaIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    ttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgtttttAclIXmnIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    agggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgatcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctSspIBspHISgrDIbla promoterAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    cggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatcgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagMfeI6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMluIBsiWIBpu10I6,3206,340
    • Plasmid
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    Editing disabled on read-only sequence.
    6349 bp450050005500600050010001500CMVTetR binding sitesTetO760-1932

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    6349