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pCAGGS-hyPBase
  • Sequence Map
    Editing disabled on read-only sequence.
    gtcgatcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacagctagctgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattPvuISpeICAG promoterCAG enhancer20406080100120140160
    cgccaatagggactttccattgacgtcaatgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctgataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCAG promoterCAG enhancer180200220240260280300320340
    gcagtacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggcgcgcgccaggcggggcgtcatgtagatgcataatcagtagcgataatggtacccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccgcgcgcggtccgccccSnaBICAG promoterCAG enhancer360380400420440460480500520
    cggggcgggggcgagggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctgccccgcccccgctcccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcaacggaagcggggcacggggcgaCAG promoter540560580600620640660680700
    ccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagggccctttgtgcggcgcggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgcgaaccaaattactgccgagcaaagaaaagacaccgacgcactttcggaatttcccgaggccctcccgggaaacacgAfeIApaIPfoIPspOMI720740760780800820840860
    gggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcccccccctcgccgagccccccacgcacgcacacacacacgcacccctcgcggcgcacgccgggcgcgacgggccgccgacactcgcgacgcccgcgccgcgccccgaaacacgcgaggcgcacacgcgctcccctcgcgccggcccccgccacggggcgccacgcccccccgacgAfeIEagI8809009209409609801,0001,0201,040
    gaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccgggcctccccttgtttccgacgcacgccccacacacgcacccccccactcgtcccccacacccgcgccgccagcccgacattggggggggacgtggggggaggggctcaacgactcgtgccgggccgaagcccacgccccgaggcacgccccgcaccgcgccccgagcggcacggcccgBlpI1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccccccccaccgccgtccacccccacggcccgccccgccccggcggagcccggcccctcccgagccccctccccgcgccgccgggggcctcgcggccgccgacagctccgcgccgctcggcgtcggtaacggaaaataccattagcacgctctcccgcgtccctgaaggaaacagggMreINaeISgrAINgoMIV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgtttagacacgcctcggctttagaccctccgcggcggcgtgggggagatcgcccgcgccccgcttcgccacgccgcggccgtccttcctttacccgcccctcccggaagcacgcagcggcgcggcggcaggggaagagggagaggtcggagccccgacaggcgcccccctgccgacNaeINgoMIV1,4201,4401,4601,4801,5001,5201,5401,560
    ccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgccgccaccATGGGggaagccccccctgccccgtcccgccccaagccgaagaccgcacactggccgccgagatcgatctcggagacgattggtacaagtacggaagaagaaaaaggatgtcgaggacccgttgcacgaccaataacacgacagagtagtaaaaccgtttcttaagcggcggtggTACCCBcgIEcoRIMG2CAG1640cag1700-seq-fwd1,5801,6001,6201,6401,6601,6801,7001,7201,740
    CAGCAGCCTGGACGACGAGCACATCCTGAGCGCCCTGCTGCAGAGCGACGACGAGCTGGTCGGCGAGGACAGCGACAGCGAGGTGAGCGACCACGTGAGCGAGGACGACGTGCAGTCCGACACCGAGGAGGCCTTCATCGACGAGGTGCACGAGGTGCAGCCTACCAGCAGCGGCGTCGTCGGACCTGCTGCTCGTGTAGGACTCGCGGGACGACGTCTCGCTGCTGCTCGACCAGCCGCTCCTGTCGCTGTCGCTCCACTCGCTGGTGCACTCGCTCCTGCTGCACGTCAGGCTGTGGCTCCTCCGGAAGTAGCTGCTCCACGTGCTCCACGTCGGATGGTCGTCGCCGAhdIPmlIStuIBmgBIBpu10IDraIIISS4LD6DE8HI10LS12AL14LQ16SD18DE20LV22GE24DS26DS28EV30SD32HV34SE36DD38VQ40SD42TE44EA46FI48DE50VH52EV54QP56TS58SG601746-3533hyPBase1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TCCGAGATCCTGGACGAGCAGAACGTGATCGAGCAGCCCGGCAGCTCCCTGGCCAGCAACAGGATCCTGACCCTGCCCCAGAGGACCATCAGGGGCAAGAACAAGCACTGCTGGTCCACCTCCAAGCCCACCAGGCGGAGCAGGGTGTCCGCCCTGAACATCGTGAGAAGCCAGAAGGCTCTAGGACCTGCTCGTCTTGCACTAGCTCGTCGGGCCGTCGAGGGACCGGTCGTTGTCCTAGGACTGGGACGGGGTCTCCTGGTAGTCCCCGTTCTTGTTCGTGACGACCAGGTGGAGGTTCGGGTGGTCCGCCTCGTCCCACAGGCGGGACTTGTAGCACTCTTCGGTCTMscIPfoISE62IL64DE66QN68VI70EQ72PG74SS76LA78SN80RI82LT84LP86QR88TI90RG92KN94KH96CW98ST100SK102PT104RR106SR108VS110AL112NI114VR116SQ1181746-3533hyPBase1,9401,9601,9802,0002,0202,0402,0602,0802,100
    GGGGCCCCACCAGGATGTGCAGGAACATCTACGACCCCCTGCTGTGCTTCAAGCTGTTCTTCACCGACGAGATCATCAGCGAGATCGTGAAGTGGACCAACGCCGAGATCAGCCTGAAGAGGCGGGAGAGCATGACCTCCGCCACCTTCAGGGACACCAACGAGGACGAGATCTACCCCGGGGTGGTCCTACACGTCCTTGTAGATGCTGGGGGACGACACGAAGTTCGACAAGAAGTGGCTGCTCTAGTAGTCGCTCTAGCACTTCACCTGGTTGCGGCTCTAGTCGGACTTCTCCGCCCTCTCGTACTGGAGGCGGTGGAAGTCCCTGTGGTTGCTCCTGCTCTAGATApaIBglIIPspOMIRG120PT122RM124CR126NI128YD130PL132LC134FK136LF138FT140DE142II144SE146IV148KW150TN152AE154IS156LK158RR160ES162MT164SA166TF168RD170TN172ED174EI176Y1746-3533hyPBase2,1202,1402,1602,1802,2002,2202,2402,260
    CGCCTTCTTCGGCATCCTGGTGATGACCGCCGTGAGGAAGGACAACCACATGAGCACCGACGACCTGTTCGACAGATCCCTGAGCATGGTGTACGTGAGCGTGATGAGCAGGGACAGATTCGACTTCCTGATCAGATGCCTGAGGATGGACGACAAGAGCATCAGGCCCACCCTGGCGGAAGAAGCCGTAGGACCACTACTGGCGGCACTCCTTCCTGTTGGTGTACTCGTGGCTGCTGGACAAGCTGTCTAGGGACTCGTACCACATGCACTCGCACTACTCGTCCCTGTCTAAGCTGAAGGACTAGTCTACGGACTCCTACCTGCTGTTCTCGTAGTCCGGGTGGGACBpu10IBsu36IA178FF180GI182LV184MT186AV188RK190DN192HM194ST196DD198LF200DR202SL204SM206VY208VS210VM212SR214DR216FD218FL220IR222CL224RM226DD228KS230IR232PT234L1746-3533hyPBase2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CGGGAGAACGACGTGTTCACCCCCGTGAGAAAGATCTGGGACCTGTTCATCCACCAGTGCATCCAGAACTACACCCCTGGCGCCCACCTGACCATCGACGAGCAGCTGCTGGGCTTCAGGGGCAGGTGCCCCTTCAGGGTCTATATCCCCAACAAGCCCAGCAAGTACGGCATCAGCCCTCTTGCTGCACAAGTGGGGGCACTCTTTCTAGACCCTGGACAAGTAGGTGGTCACGTAGGTCTTGATGTGGGGACCGCGGGTGGACTGGTAGCTGCTCGTCGACGACCCGAAGTCCCCGTCCACGGGGAAGTCCCAGATATAGGGGTTGTTCGGGTCGTTCATGCCGTAGTAleIBglIIBmgBIR236EN238DV240FT242PV244RK246IW248DL250FI252HQ254CI256QN258YT260PG262AH264LT266ID268EQ270LL272GF274RG276RC278PF280RV282YI284PN286KP288SK290YG292I1746-3533hyPBase2,4602,4802,5002,5202,5402,5602,5802,6002,620
    AGATCCTGATGATGTGCGACAGCGGCACCAAGTACATGATCAACGGCATGCCCTACCTGGGCAGGGGCACCCAGACCAACGGCGTGCCCCTGGGCGAGTACTACGTGAAGGAGCTGTCCAAGCCCGTCCACGGCAGCTGCAGAAACATCACCTGCGACAACTGGTTCACCAGCATTCTAGGACTACTACACGCTGTCGCCGTGGTTCATGTACTAGTTGCCGTACGGGATGGACCCGTCCCCGTGGGTCTGGTTGCCGCACGGGGACCCGCTCATGATGCACTTCCTCGACAGGTTCGGGCAGGTGCCGTCGACGTCTTTGTAGTGGACGCTGTTGACCAAGTGGTCGTAPasIScaISphIK294IL296MM298CD300SG302TK304YM306IN308GM310PY312LG314RG316TQ318TN320GV322PL324GE326YY328VK330EL332SK334PV336HG338SC340RN342IT344CD346NW348FT350SI3521746-3533hyPBase2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCCCCTGGCCAAGAACCTGCTGCAGGAGCCCTACAAGCTGACCATCGTGGGCACCGTGAGAAGCAACAAGAGAGAGATCCCCGAGGTCCTGAAGAACAGCAGGTCCAGGCCCGTGGGCACCAGCATGTTCTGCTTCGACGGCCCCCTGACCCTGGTGTCCTACAAGCCCAAGCCCGGGGGACCGGTTCTTGGACGACGTCCTCGGGATGTTCGACTGGTAGCACCCGTGGCACTCTTCGTTGTTCTCTCTCTAGGGGCTCCAGGACTTCTTGTCGTCCAGGTCCGGGCACCCGTGGTCGTACAAGACGAAGCTGCCGGGGGACTGGGACCACAGGATGTTCGGGTTCGGGAloIMscIXcmIBstXIEcoNIPL354AK356NL358LQ360EP362YK364LT366IV368GT370VR372SN374KR376EI378PE380VL382KN384SR386SR388PV390GT392SM394FC396FD398GP400LT402LV404SY406KP408KP4101746-3533hyPBase2,8202,8402,8602,8802,9002,9202,9402,960
    GCCAAGATGGTGTACCTGCTGTCCAGCTGCGACGAGGACGCCAGCATCAACGAGAGCACCGGCAAGCCCCAGATGGTGATGTACTACAACCAGACCAAGGGCGGCGTGGACACCCTGGACCAGATGTGCAGCGTGATGACCTGCAGCAGAAAGACCAACAGGTGGCCCATGGCCCCGGTTCTACCACATGGACGACAGGTCGACGCTGCTCCTGCGGTCGTAGTTGCTCTCGTGGCCGTTCGGGGTCTACCACTACATGATGTTGGTCTGGTTCCCGCCGCACCTGTGGGACCTGGTCTACACGTCGCACTACTGGACGTCGTCTTTCTGGTTGTCCACCGGGTACCGGGXcmIPflMIAK412MV414YL416LS418SC420DE422DA424SI426NE428ST430GK432PQ434MV436MY438YN440QT442KG444GV446DT448LD450QM452CS454VM456TC458SR460KT462NR464WP466MA4681746-3533hyPBase2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TGCTGTACGGCATGATCAACATCGCCTGCATCAACAGCTTCATCATCTACAGCCACAACGTGAGCAGCAAGGGCGAGAAGGTGCAGAGCCGGAAAAAGTTCATGCGGAACCTGTACATGGGCCTGACCTCCAGCTTCATGAGGAAGAGGCTGGAGGCCCCCACCCTGAAGAGATAACGACATGCCGTACTAGTTGTAGCGGACGTAGTTGTCGAAGTAGTAGATGTCGGTGTTGCACTCGTCGTTCCCGCTCTTCCACGTCTCGGCCTTTTTCAAGTACGCCTTGGACATGTACCCGGACTGGAGGTCGAAGTACTCCTTCTCCGACCTCCGGGGGTGGGACTTCTCTATXmnIBsaBIBsrGIDraIIILL470YG472MI474NI476AC478IN480SF482II484YS486HN488VS490SK492GE494KV496QS498RK500KF502MR504NL506YM508GL510TS512SF514MR516KR518LE520AP522TL524KR526Y1746-3533hyPBase3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCTGAGGGACAACATCAGCAACATCCTGCCCAAAGAGGTGCCCGGCACCAGCGACGACAGCACCGAGGAGCCCGTGATGAAGAAGAGGACCTACTGCACCTACTGTCCCAGCAAGATCAGAAGAAAGGCCAGCGCCAGCTGCAAGAAGTGTAAGAAGGTCATCTGCCGGGAGCACGGACTCCCTGTTGTAGTCGTTGTAGGACGGGTTTCTCCACGGGCCGTGGTCGCTGCTGTCGTGGCTCCTCGGGCACTACTTCTTCTCCTGGATGACGTGGATGACAGGGTCGTTCTAGTCTTCTTTCCGGTCGCGGTCGACGTTCTTCACATTCTTCCAGTAGACGGCCCTCGTGBarIBsu36IL528RD530NI532SN534IL536PK538EV540PG542TS544DD546ST548EE550PV552MK554KR556TY558CT560YC562PS564KI566RR568KA570SA572SC574KK576CK578KV580IC582RE584H1746-3533hyPBase3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AACATCGACATGTGCCAGAGCTGTTTCTGATGAgcggccgcggttctagataagtcgacggatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtTTGTAGCTGTACACGGTCTCGACAAAGACTACTcgccggcgccaagatctattcagctgcctagaaaaagggagacggtttttaatacccctgtagtacttcggggaactcgtagactgaagaccgattatttcctttaaataaaagtaacgttatcacacaaccttaaaaaacaAccIEagINotISalIXbaIHincIIN586ID588MC590QS592CF594**5961746-3533hyPBasecaggs-seq-revCAGGS-R23,5203,5403,5603,5803,6003,6203,6403,660
    gtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagcagagagtgagccttcctgtataccctcccgtttagtaaattttgtagtcttactcataaaccaaatctcaaaccgttgtatacgggtatacgaccgacggtacttgtttccaaccgatatttctccagtagtcatatactttgtcgggggacgacaggtaaggaataaggtatcAjuIPflMI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    aaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgcagcccttttcggaactgaactccaatctaaaaaaaatataaaacaaaacacaataaaaaaagaaattgtagggattttaaaaggaatgtacaaaatgatcggtctaaaaaggaggagaggactgatgagggtcagtatcgacagggagaagagaatacctctagggagctggacgtcggg3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgttcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacHindIIIlac promoterLacOM13-rev4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tcgtgccagcggatccgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtaagcacggtcgcctaggcgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagccggagactcgataaggtcttcatBplIBplISfiIBsaBISV40 Ori4,2204,2404,2604,2804,3004,3204,3404,360
    gtgaggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggacactcctccgaaaaaacctccggatccgaaaacgtttttcgattgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacctBsmIPsiIStuIAvrIISV40 Ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tccgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaggcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctSapIBsaXIBspQI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    aagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgaColE1 origin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctggggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagttacgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacBciVIColE1 origin4,9204,9404,9604,9805,0005,0205,0405,060
    cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgColE1 origin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcColE1 origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcAmp_RColE1 origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttaaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaatAhdIBsaIAmp_R5,6205,6405,6605,6805,7005,7205,7405,760
    tccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccaggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggAclIFspIAmp_R5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactctPvuIScaIAmp_R5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgAclIBcgIXmnIAmp_R6,1406,1606,1806,2006,2206,2406,2606,2806,300
    tgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataSspIBciVIAmp_R6,3206,3406,3606,3806,4006,4206,4406,460
    ttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacSexAI6,4806,5006,5206,540
    • Plasmid
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    6541 bp450050005500600050010001500CAG promoterCAG enhancer

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    6541