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CS2/CMV-nGRAPHIC-T2A-H2B-mCherry
  • Sequence Map
    Editing disabled on read-only sequence.
    gacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagaMfeI20406080100120140160
    gcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaaccgaatcccaatccgcaaaacgcgacgaagcgctacatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgMluINruISpeICMV enhancer180200220240260280300320340
    gacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaNdeICMV enhancer360380400420440460480500520
    ggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaCspCISnaBICMV enhancer540560580600620640660680700
    ttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtcgcgcaaaacggacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttggg720740760780800820840860880
    actgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgactgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcaccgcgggcttgtccctgaactttcgctttccctttggtctcctcgagagagctgAflII9009209409609801,0001,0201,0401,060
    gcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaacgtcctgagccgaacgacttcgcgcgtgccgttctccgctccccgccgctgaccactcatgcggtttttaaaactgatcgcctccgatcttcctctctctacccacgctctcgcagtcataattcgccccctcttaatctagcgctacccttttttaagccaattccggtccccctttNruIBssHII1,0801,1001,1201,1401,1601,1801,2001,2201,240
    gaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctccttttttatatttaattttgtatatcatacccgttcgtccctcgatcttgctaagcgtcaattaggaccggacaatctttgtagtcttccgacatctgtttatgaccctgtcgatgttggtagggaagtctgtcctagtcttcttgaatctagtaatatattatgtcatcgttgggag1,2601,2801,3001,3201,3401,3601,3801,4001,420
    tattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtataacacacgtagtttcctatctctattttctgtggttccttcgaaatctgttctatctccttctcgttttgttttcattctggtggcgtgtcgttcgccggccggcgactagaagtctggacctcctcctctatactccctgttaacctcttcacttaatatatttatatttcatcaFseIMfeISapIBspQI1,4401,4601,4801,5001,5201,5401,5601,5801,600
    aaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatatttttaacttggtaatcctcatcgtgggtggttccgtttctcttctcaccacgtctctcttttttctcgtcacccttatcctcgaaacaaggaacccaagaaccctcgtcgtccttcgtgatacccgcgtcgcagttactgcgactgccatgtccggtctgttaataacagaccatat1,6201,6401,6601,6801,7001,7201,7401,7601,780
    gtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgcacgtcgtcgtcttgttaaacgactcccgataactccgcgttgtcgtagacaacgttgagtgtcagaccccgtagttcgtcgaggtccgttcttaggaccgacacctttctatggatttcctagttgtcgaggacccctaaaccccaacgagaccttttgagtaaacgtggtgacgacBbvCI1,8001,8201,8401,8601,8801,9001,9201,940
    tgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcacggaaccttacgatcaacctcattatttagagaccttgtctaaaccttagtgtgctggacctacctcaccctgtctctttaattgttaatgtgttcgaattatgtgaggaattaacttcttagcgttttggtcgttcttttcttacttgttcttaataaccttaatctatttacccgBsmI1,9601,9802,0002,0202,0402,0602,0802,1002,120
    aagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggattcaaacaccttaaccaaattgtattgtttaaccgacaccatatattttaataagtattactatcatcctccgaaccatccaaattcttatcaaaaacgacatgaaagatatcacttatctcaatccgtccctataagtggtaatagcaaagtctgggtggagggttggggctcccctKflIPpuMI2,1402,1602,1802,2002,2202,2402,2602,2802,300
    cccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaagggctgtccgggcttccttatcttcttcttccacctctctctctgtctctgtctaggtaagctaatcacttgcctagatgtttaccgtcataagtaggtgttaaaattttcttttcccccctaaccccccatgtcacgtcccctttcttatcatctgtattatcgttgtctgtatgtt2,3202,3402,3602,3802,4002,4202,4402,4602,480
    actaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagaaattcactttgaattaattcgattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgtgatttcttaatgtttttgtttaatgtttttaagttttaaaagcccaaataatgtccctgtcgtctttaagtgaaacttaattaagctaattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgacCMV enhancer2,5002,5202,5402,5602,5802,6002,6202,6402,660
    accgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttNdeICMV enhancer2,6802,7002,7202,7402,7602,7802,8002,8202,840
    tggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttacCspCISnaBICMV enhancerCMV promoter2,8602,8802,9002,9202,9402,9602,9803,0003,020
    ggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagagccaccatggtagagatgctgccaactgtcgctgcctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgaccaaatcacttggcagtctaggcgatctcggtggtaccatctctacgacggttgacagcgacCMV promoterSP (membran...roacrosin)3,0403,0603,0803,1003,1203,1403,1603,1803,200
    tgctggtcttggcagtgtccgtggttgccaaggataacaccacgctgcaggctagagtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgcgcggcgagggcgagggcgatgccaccatcggcaaacgaccagaaccgtcacaggcaccaacggttcctattgtggtgcgacgtccgatctcactcgttcccgctcctcgacaagtggccccaccacgggtaggaccagctcgacctgccgctgcatttgccggtgttcaagtcgcacgcgccgctcccgctcccgctacggtggtagccgttBcgIPstIGFP1_7SP (membrane transport, murine preproacrosin)3,2203,2403,2603,2803,3003,3203,3403,3603,380
    gctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatctccttccgactgggacttcaagtagacgtggtggccgttcgacgggcacgggaccgggtgggagcactggtgggactggatgccgcacgtcacgaagtcggcgatggggctggtgtacttcgtcgtgctgaagaagttcaggcggtacgggcttccgatgcaggtcctcgcgtggtagaggaagGFP1_73,4003,4203,4403,4603,4803,5003,5203,5403,560
    aaggacgacggcaaatacaagacccgcgccgttgtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcaccgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaacgtctatatcaccgccgacaagcaggctagagttcctgctgccgtttatgttctgggcgcggcaacacttcaagctcccgctgtgggaccacttggcgtagctcgacttcccgtggctgaagttcctcctgccgttgtaggaccccgtgttcgacctcatgttgaagttgtcggtgttgcagatatagtggcggctgttcgtccgatctcGFP1_73,5803,6003,6203,6403,6603,6803,7003,720
    gtggaggcggttcaggcggaggtggctctggcggtggcggatcggctagcgccaccatggcccagctcgaaaaggagctgcaagccctggagaaggagaacgcccagctcgaatgggagctccaggccctggagaaggagctggcccagaaggctagcggtggaggcggttcaggcggcacctccgccaagtccgcctccaccgagaccgccaccgcctagccgatcgcggtggtaccgggtcgagcttttcctcgacgttcgggacctcttcctcttgcgggtcgagcttaccctcgaggtccgggacctcttcctcgaccgggtcttccgatcgccacctccgccaagtccgccBmtIBmtINheINheILeucin zipper AGGGGS x 3LZA7GGGGS x 33,7403,7603,7803,8003,8203,8403,8603,8803,900
    aggtggctctggcggtggcggatcgtctagcggtggaggcggttcaggcggaggtggctctggcggtggcggatcgtctagcggtggaggcggttcaggcggaggtggctctggcggtggcggatcgtctagctaccaactcgagaatggcggcataagcctgctggttcagaacacatccaccgagaccgccaccgcctagcagatcgccacctccgccaagtccgcctccaccgagaccgccaccgcctagcagatcgccacctccgccaagtccgcctccaccgagaccgccaccgcctagcagatcgatggttgagctcttaccgccgtattcggacgaccaagtcttgtgtTliIXcmIXhoIPaeR7IGGGGS x 3GGGGS x 3CD90 GPI anchor domainGGGGS x 33,9203,9403,9603,9804,0004,0204,0404,0604,080
    tcctggatgctgctgctgctgctttccctctccctcctccaagccctggacttcatttctctgtctagcgagggcagaggaagtcttctaacatgcggtgacgtcgaggagaatcctggcccaatgccagagccagcgaagtctgctcccgccccgaaaaagggctccaagaaggcggaggacctacgacgacgacgacgaaagggagagggaggaggttcgggacctgaagtaaagagacagatcgctcccgtctccttcagaagattgtacgccactgcagctcctcttaggaccgggttacggtctcggtcgcttcagacgagggcggggctttttcccgaggttcttccgccCD90 GPI anchor domainhuman H2BT2A4,1004,1204,1404,1604,1804,2004,2204,2404,260
    tgactaaggcgcagaagaaaggcggcaagaagcgcaagcgcagccgcaaggagagctattccatctatgtgtacaaggttctgaagcaggtccaccctgacaccggcatttcgtccaaggccatgggcatcatgaattcgtttgtgaacgacattttcgagcgcatcgcaggtgaggcactgattccgcgtcttctttccgccgttcttcgcgttcgcgtcggcgttcctctcgataaggtagatacacatgttccaagacttcgtccaggtgggactgtggccgtaaagcaggttccggtacccgtagtacttaagcaaacacttgctgtaaaagctcgcgtagcgtccactccgBfuAIBfuAIBspMIBspMIBsrGIBstXIEcoRIPaqCIhuman H2B4,2804,3004,3204,3404,3604,3804,4004,4204,440
    ttcccgcctggcgcattacaacaagcgctcgaccatcacctccagggagatccagacggccgtgcgcctgctgctgcctggggagttggccaagcacgccgtgtccgagggtactaaggccatcaccaagtacaccagcgctaaggatccagccaccatggtgagcaagggcgaggagaagggcggaccgcgtaatgttgttcgcgagctggtagtggaggtccctctaggtctgccggcacgcggacgacgacggacccctcaaccggttcgtgcggcacaggctcccatgattccggtagtggttcatgtggtcgcgattcctaggtcggtggtaccactcgttcccgctcctcAfeIAfeIBamHIhuman H2BmCherry4,4604,4804,5004,5204,5404,5604,5804,6004,620
    gataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtctattgtaccggtagtagttcctcaagtacgcgaagttccacgtgtacctcccgaggcacttgccggtgctcaagctctagctcccgctcccgctcccggcggggatgctcccgtgggtctggcggttcgacttccactggttcccaccgggggacgggaagcggaccctgtaggacaAhdIBstEIImCherry4,6404,6604,6804,7004,7204,7404,7604,7804,800
    cccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggggagtcaagtacatgccgaggttccggatgcacttcgtggggcggctgtaggggctgatgaacttcgacaggaaggggctcccgaagttcaccctcgcgcactacttgaagctcctgccgccgcaccactggcactgggtcctgaggagggacgtcctgccgctcaagtagatgttPstISbfIBstEIImCherry4,8204,8404,8604,8804,9004,9204,9404,9604,980
    ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccccacttcgacgcgccgtggttgaaggggaggctgccggggcattacgtcttcttctggtacccgaccctccggaggaggctcgcctacatggggctcctgccgcgggacttcccgctctagttcgtctccgacttcgacttcctgccgccggtgatgctgcgactccagttctggtggAloIBbvCImCherry5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaatctagagttaacatcgagggatatgttccggttcttcgggcacgtcgacgggccgcggatgttgcagttgtagttcaacctgtagtggagggtgttgctcctgatgtggtagcaccttgtcatgcttgcgcggctcccggcggtgaggtggccgccgtacctgctcgacatgttcattagatctcaattgtagctccctaHpaIXbaIXcmIBsrGISgrAImCherry5,1805,2005,2205,2405,2605,2805,3005,3205,340
    caagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctgttcgaatagctattagttggagacctaatgttttaaacactttctaactgaccataagaattgatacaacgaggaaaatgcgatacacctatgcgacgaaattacggaaacatagtacgataacgaagggcataccgaaagtaaaagaggaggaacatatttaggaccaacgacagaClaIBspDI5,3605,3805,4005,4205,4405,4605,4805,500
    ctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctgaaatactcctcaacaccgggcaacagtccgttgcaccgcaccacacgtgacacaaacgactgcgttgggggtgaccaaccccgtaacggtggtggacagtcgaggaaaggccctgaaagcgaaagggggagggataacggtgccgccttgagtagcggcggacggaacgggcgacga5,5205,5405,5605,5805,6005,6205,6405,6605,680
    ggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctcctgtccccgagccgacaacccgtgactgttaaggcaccacaacagccccttcgactgcaggaaaggtaccgacgagcggacacaacggtggacctaagacgcgccctgcaggaagacgatgcagggaagccgggagttaggtcgcctggaaggaagggcgccggacgacggccgagaSacII5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggtcgccggagaaggcgcagaagcggaagcgggagtctgctcagcctagagggaaacccggcggaggggcgtagctatggcagctctggatctttttgtacctcgttagtgttcatcgttatgtcgtcgatggttacgactaacacggaccgatcttcgtgttctcctcctcctccacccaClaIBspDI5,8805,9005,9205,9405,9605,9806,0006,0206,040
    tttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactaaaggtcagtgtggagtccatggaaattctggttactgaatgttccgtcgacatctagaatcggtgaaaaattttcttttcccccctgaccttcccgattaagtgagggttgcttctgttctataggaactagacacctagatggtgtgtgttccgatgaagggactaaccgtcttgaKpnIEcoRVAcc65IBsu36I6,0606,0806,1006,1206,1406,1606,1806,2006,220
    acacaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgatgtgtggtcccggtccctagtctataggtgactggaaacctaccacgatgttcgatcatggtcaactcgttctcttccatcttcttcggttacttcctctcttgtgggcgaacaatgtgggacactcggacgtaccctacctactgggcctctctcttcataatctcacctccaaactEcoRV6,2406,2606,2806,3006,3206,3406,3606,3806,400
    cagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgacgtcggcggatcgtaaagtagtgtaccgggctctcgacgtaggcctgacatgacccagagagaccaatctggtctagactcggaccctcgagagaccgattgatcccttgggtgacgaattcggagttatttcgaacggaactcacgaagttcatcacacacgggcagacaacacactgAflIIBspEI6,4206,4406,4606,4806,5006,5206,5406,5606,580
    tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaaagaccattgatctctagggagtctgggaaaatcagtcacaccttttagagatcgtcccgggcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattApaIBclIPmeIPspOMI6,6006,6206,6406,6606,6806,7006,7206,7406,760
    taaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccatagggg6,7806,8006,8206,8406,8606,8806,9006,9206,940
    acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaF1 ori6,9606,9807,0007,0207,0407,0607,0807,1007,120
    tagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaAloIF1 ori7,1407,1607,1807,2007,2207,2407,2607,280
    ttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaattaagacaccttacacacagtcaatcccacacctttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtccacaccNsiIPsiISexAI7,3007,3207,3407,3607,3807,4007,4207,4407,460
    aaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcttttcaggggtccgaggggtcgtccgtcttcatacgtttcgtacgtagagttaatcagtcgttggtatcagggcggggattgaggcgggtagggcggggattgaggcgggtcaaggcgggtaagaggcggggtaccgactgattaaaaaaaataaatacgtctccggctccggcggagaNsiI7,4807,5007,5207,5407,5607,5807,6007,6207,640
    gcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccggagactcgataaggtcttcatcactcctccgaaaaaacctccggatccgaaaacgtttttcgagggccctcgaacatataggtaaaagcctagactagtcgtgcacaactgttaattagtagccgtatcatatagccgtatcatattatgctgttccactccttgatttggtaccgBclIPmlISmaIXmaIAvrIITspMI7,6607,6807,7007,7207,7407,7607,7807,8007,820
    caagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacgttcaactggtcacggcaaggccacgagtggcgcgcgctgcagcggcctcgccagctcaagacctggctggccgagcccaagagggccctgaagcacctcctgctgaagcggccacaccaggccctgctgcactgggacaagtagtcgcgccaggtcctggtccaccacggcctgttgSmaIXmaIBmgBIMauBISexAISgrAITspMIBssHII7,8407,8607,8807,9007,9207,9407,9607,9808,000
    accctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgtgggaccggacccacacccacgcgccggacctgctcgacatgcggctcaccagcctccagcacaggtgcttgaaggccctgcggaggcccggccggtactggctctagccgctcgtcggcacccccgccctcaagcgggacgcgctgggccggccgttgacgcacgtgaagcaccggcFseI8,0208,0408,0608,0808,1008,1208,1408,1608,180
    aggagcaggactgacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaatatcctcgtcctgactgtgcacgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatPmlIPsiI8,2008,2208,2408,2608,2808,3008,3208,3408,360
    aagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaBsmISalIBstZ17IM13-revLacO8,3808,4008,4208,4408,4608,4808,5008,5208,540
    ccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgalac promoter8,5608,5808,6008,6208,6408,6608,6808,7008,720
    cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgPciISapIBspQIColE...gin8,7408,7608,7808,8008,8208,8408,8608,8808,900
    gtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcColE1 origin8,9208,9408,9608,9809,0009,0209,0409,060
    ggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcColE1 origin9,0809,1009,1209,1409,1609,1809,2009,2209,240
    ccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccColE1 origin9,2609,2809,3009,3209,3409,3609,3809,4009,420
    tagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaaatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttColE1 origin9,4409,4609,4809,5009,5209,5409,5609,5809,600
    tgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacAhdIAmpR9,6209,6409,6609,6809,7009,7209,7409,7609,780
    caatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgtgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaAclIFspIAmpR9,8009,8209,8409,8609,8809,9009,9209,9409,960
    tgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatgacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtacPvuIAmpR9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcacaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtBcgIScaIAmpR10,16010,18010,20010,22010,24010,26010,28010,30010,320
    tcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgAclI10,34010,36010,38010,40010,42010,44010,46010,48010,500
    acggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagSalISspISgrDI10,52010,54010,56010,58010,60010,62010,640
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10659 bp75008000850090009500100005001000150020002500CMV enhancerCMV enhancer,MfeI,NruI,MluI,SpeI,NdeI,SnaBI,CspCIAflIIBssHII,NruIBspQI,SapI,FseI,MfeIBbvCIBsmIKflIPpuMIFseIPmlI,PsiIM13-rev,LacO,lac promoter,BsmI,BstZ17I,SalIBspQI,SapIPciIColE1 originAmpR,AhdI,FspI,AclIPvuI,ScaI,BcgI,AclI,SspI,SalI,SgrDI

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    10659