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LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHI*W286LSM041pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttBsaIBsrDIHK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228171-1031Amp (bla)LSM041180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIBglIFspIBsrDIWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170171-1031Amp (bla)LSM041360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattPvuIEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L171-1031Amp (bla)LSM041540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K171-1031Amp (bla)LSM041720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M171-1031Amp (bla)bla promoterLSM0418809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactBglIIBspHISgrDIbla promoterLSM0411,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaMfeISFFVLSM0411,2401,2601,2801,3001,3201,3401,3601,3801,400
    tatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggBmtINheISmaIXmaIEcoRVTspMISFFVLSM0411,4201,4401,4601,4801,5001,5201,5401,560
    gcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttccgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagAflIIBbvCIBpu10IBssHIISFFVLSM0411,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCAleIAscISacISacISmaIXmaITspMIBssHIIEco53kIEco53kIHindIIIMV2SK4GE6ED8NM10AI12IK14EF16MR18FK20VH22ME24GS26VN281841-3623M1mCherrySFFVLSM0411,7601,7801,8001,8201,8401,8601,8801,9001,920
    GCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGH30EF32EI34EG36EG38EG40RP42YE44GT46QT48AK50LK52VT54KG56GP58LP60FA62WD64IL66SP68QF70MY72GS74KA76YV78KH80PA82DI84PD86Y1841-3623mCherryLSM0411,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGAloISbfIL88KL90SF92PE94GF96KW98ER100VM102NF104ED106GG108VV110TV112TQ114DS116SL118QD120GE122FI124YK126VK128LR130GT132NF134PS136DG138PV140MQ142KK144T1841-3623mCherryLSM0412,1202,1402,1602,1802,2002,2202,2402,260
    ATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCABbsIBplIBplIBsgIKasIKasINarINarISfoISfoIBbvCIPflMIPluTIPluTIBpu10IM146GW148EA150SS152ER154MY156PE158DG160AL162KG164EI166KQ168RL170KL172KD174GG176HY178DA180EV182KT184TY186KA188KK190PV192QL194PG196AY198NV200NI202K1841-3623mCherryLSM0412,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTAleIMluIXcmIBsrGISgrAIAflIIIL204DI206TS208HN210ED212YT214IV216EQ218YE220RA222EG224RH226ST228GG230MD232EL234YK236SD238AL240EA242RH244KQ246KI248VA250PV252KQ254TL256NF258DL260LK2621841-3623mCherry5' StopGo (2A; Loughran 2017)LSM0412,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAAAGGAGTTTATATGGAAACCCTTCTACGGAGTTTATATGGAAACCCGTTCTTCGACATTATATGGAATGCGAATAGTATCCCACCTACCCAGACGGaccggtactCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTTCCTCAAATATACCTTTGGGAAGATGCCTCAAATATACCTTTGGGCAAGAAGCTGTAATATACCTTACGCTTATCATAGGGTGGATGGGTCTGCCtggccatgaAgeIBsmIKpnISpeIXmnIBamHIBsmBIEsp3IRsrIIAcc65ILA264GD266VE268SN270PG272PG274TG276SS278V*280VG282GI284VK286EF288IW290KP292FY294GV296YM298ET300RS302ST304LY306GM308RI310VS312HL314PR316RT318GT3201841-36233xPP75' StopGo (2A; Loughran 2017)5' stem3' primeLSM0412,6402,6602,6802,7002,7202,7402,7602,7802,800
    agtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTgtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggtcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAcacagattcccgcttctcgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcaccSapITliIXhoIBfuAIBspMIBspQIBsrGIPaqCIPspXIPaeR7ISE322AR324HK326QK328IV330AP332VK334QT336LN338FD340LL342KL344AG346DV348ES350NP352GP354SS356VS358KG360EE362LI364KE366NM368HM370KL372YM374EG376TV3781841-36233' StopGo (2A; Loughran 2017)XZ076mtagBFP2LSM0412,8202,8402,8602,8802,9002,9202,9402,960
    acaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgatgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctPasIDN380HH382FK384CT386SE388GE390GK392PY394EG396TQ398TM400RI402KV404VE406GG408PL410PF412AF414DI416LA418TS420FL422YG424SK426TF428IN430HT432QG434IP436D1841-3623XZ076mtagBFP22,9803,0003,0203,0403,0603,0803,1003,1203,140
    cttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaagaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagttctagtctccccacttgaagtgtaggttgccgggacactacgtcttctttAloIBsu36IF438FK440QS442FP444EG446FT448WE450RV452TT454YE456DG458GV460LT462AT464QD466TS468LQ470DG472CL474IY476NV478KI480RG482VN484FT486SN488GP490VM492QK494K1841-3623XZ076mtagBFP23,1603,1803,2003,2203,2403,2603,2803,3003,320
    acactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatatctaggttctttgggcgattcttggagttctacggaccgcagatgatacBglIT496LG498WE500AF502TE504TL506YP508AD510GG512LE514GR516ND518MA520LK522LV524GG526SH528LI530AN532AK534TT536YR538SK540KP542AK544NL546KM548PG550VY552Y1841-3623XZ076mtagBFP23,3403,3603,3803,4003,4203,4403,4603,4803,500
    tggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAgccggagggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTcggcctcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctApaIPacIPspOMIV554DY556RL558ER560IK562EA564NN566ET568YV570EQ572HE574VA576VA578RY580CD582LP584SK586LG588HK590LN592*A5941841-3623XZ076mtagBFP2c-myc epitopeLSM0413,5203,5403,5603,5803,6003,6203,6403,660
    ccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccBclIPmeIBtgZIBGH polyadenylation sequencePolyhi...e tagLSM0413,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccaBbsIClaISphIBglIIBspDIBGH polyadenylation sequenceBGH pALSM0413,8603,8803,9003,9203,9403,9603,9804,0004,020
    atccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggtaggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccKflINaeINgoMIVLSM0414,0404,0604,0804,1004,1204,1404,1604,1804,200
    ttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagtaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaBarIXbaIPshAIHygromycinFRTLSM0414,2204,2404,2604,2804,3004,3204,3404,360
    tcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcaBsmBIEsp3IHygromycinLSM0414,3804,4004,4204,4404,4604,4804,5004,5204,540
    gcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtPvuIAsiSIBfuAIBspMIEcoRIBstAPIHygromycinLSM0414,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcaccaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggNdeIRsrIIHygromycinLSM0414,7404,7604,7804,8004,8204,8404,8604,8804,900
    tcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggagagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctcAjuIBtgZIBstAPIHygromycinLSM0414,9204,9404,9604,9805,0005,0205,0405,060
    gcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcggSacIIHygromycinLSM0415,0805,1005,1205,1405,1605,1805,2005,2205,240
    cgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctgcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgaNaeIScaINgoMIVHygromycinLSM0415,2605,2805,3005,3205,3405,3605,3805,4005,420
    ggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtBsmIPsiISV40 pALSM0415,4405,4605,4805,5005,5205,5405,5605,5805,600
    tgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcaacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtBstZ17ILSM0415,6205,6405,6605,6805,7005,7205,7405,760
    ctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgSapIBspQILSM0415,7805,8005,8205,8405,8605,8805,9005,9205,940
    ggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgaPciIAflIIILSM041pUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgttatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacaLSM041pUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacgcacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgcLSM041pUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    gctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttLSM041pUC ori6,4806,5006,5206,5406,5606,5806,6006,620
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6636 bp450050005500600050010001500LSM041SFFVG->A from pSLQ2349171-1031EKo152.2 (DNA)EKo412.2 (DNA)EKo271.3 (DNA)EKo309.1 (DNA)EKo326.2 (DNA)Cit_1 (DNA)pNSK019 (DNA)EKo260.2 (DNA)EKo387.3 (DNA)oLSM0104 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)EKo152.1 (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)BspHIBsaI,BsrDI,BglIFspI,AclI,BsrDIAmp (bla)PvuIScaI,BcgIAclIXmnISspIbla promoter,BspHISgrDIBglIIFlpInF1 (DNA)FlpInF (DNA),EKo285.1 (DNA),MfeIEWo118.11 (DNA),GRo003.4 (DNA),+2missing in pSLQ2349missing in pSLQ2349,NheI,BmtIA->T from pSLQ2349EcoRV,TspMI,XmaI,SmaIBbvCIBpu10I,AflIIBtgZIAjuISacIIHyg_F (DNA)ScaINgoMIV,NaeISV40PA_R (DNA),PsiISV40 pA,SV40pA-R Genewiz (DNA),BsmIBstZ17IM13R Genewiz (DNA)BspQI,SapIpUCori5F (DNA)AflIIIPciIpUC ori

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    6636

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