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PX459_Nelfb sgRNA
  • Sequence Map
Editing disabled on read-only sequence.
gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactctcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaaccttaattaaactgacatttgtgtttctataatcatgttttatgcactgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgaU6 promoterhU6-F20406080100120140160
atcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaaCACCGTTCTCCACAGCCTCACAGTGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaacacctttcctgctttGTGGCAAGAGGTGTCGGAGTGTCACcaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaNdeIConfirmed by seqgRNA scaffoldU6 promoterNelfb sgRNALKO.1 5'180200220240260280300320340
ttgttttagagctagaaatagcaagttaaaataaggctagtccgtttttagcgcgtgcgccaattctgcagacaaatggctctagaggtacccgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaatagtaacgccaatagaacaaaatctcgatctttatcgttcaattttattccgatcaggcaaaaatcgcgcacgcggttaagacgtctgtttaccgagatctccatgggcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttatcattgcggttatcKpnIXbaIAcc65IConfirmed by seqCMV enhancer360380400420440460480500520
ggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattgtgcccagtacatgaccttatgggactttcctacttggcagtacatcctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaacacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtaNdeIConfirmed by seqCMV enhancer540560580600620640660680700
ctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcgggatgcataatcagtagcgataatggtaccagctccactcggggtgcaagacgaagtgagaggggtagaggggggggaggggtgggggttaaaacataaataaataaaaaattaataaaacacgtcgctacccccgccccccccccccccccgcgcgcggtccgccccgccccgccNcoISnaBIchicken beta-actin promoterConfirmed by seqCMV enhancer720740760780800820840860
ggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgctccccgccccgccccgctccgcctctccacgccgccgtcggttagtctcgccgcgcgaggctttcaaaggaaaataccgctccgccgccgccgccgccgggatatttttcgcttcgcgcgccgcccgccctcagcgacgcgcgacggaagcggggcacggggcgaggcggcchicken beta-actin promoterhybrid intron8809009209409609801,0001,0201,040
ccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagctgagcaagaggtaagggtttaagggatggttggttggtggggtattaatgtttaattacctggagcacctgcctgaaatcacggcggagcgcggcgggcggggccgagactgactggcgcaatgagggtgtccactcgcccgccctgccgggaagaggaggcccgacattaatcgactcgttctccattcccaaattccctaccaaccaaccaccccataattacaaattaatggacctcgtggacggactttagtgBseRIPaqCIhybrid intron1,0601,0801,1001,1201,1401,1601,1801,2001,220
tttttttcaggttggaccggtgccaccatggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacaaaaaaaagtccaacctggccacggtggtacctgatattcctggtgctgcctctgatgttcctagtactataactaatgtttctgctactgctattctaccggggtttcttcttcgccttccagccataggtgcctcagggtcgtcggctgttcttcatgtcgtagccggacctgtAgeINcoI3xFLAGCas9SV40 NLShy...nve...)1,2401,2601,2801,3001,3201,3401,3601,3801,400
tcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagagccgtggttgagacacccgacccggcactagtggctgctcatgttccacgggtcgttctttaagttccacgacccgttgtggctggccgtgtcgtagttcttcttggactagcctcgggacgacaagctgtcgccgctttgtcggctccggtgggccgacttctcttggcggtcBstXICas91,4201,4401,4601,4801,5001,5201,5401,560
aagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggccttcttctatgtggtctgccttcttggcctagacgatagacgttctctagaagtcgttgctctaccggttccacctgctgtcgaagaaggtgtctgaccttctcaggaaggaccaccttctcctattcttcgtgctcgccgtggggtagaagccgttgtagcacctgctccaccggBglIIPflMICas91,5801,6001,6201,6401,6601,6801,7001,7201,740
taccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcaatggtgctcttcatggggtggtagatggtggactctttctttgaccacctgtcgtggctgttccggctggacgccgactagatagaccgggaccgggtgtactagttcaaggccccggtgaaggactagctcccgctggacttggggctgttgtcgctgcacctgttcgacaagtBmgBIEcoNICas91,7601,7801,8001,8201,8401,8601,8801,9001,920
tccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctaggtcgaccacgtctggatgttggtcgacaagctccttttggggtagttgcggtcgccgcacctgcggttccggtaggacagacggtctgactcgttctcgtctgccgaccttttagactagcgggtcgacgggccgctcttcttcttaccggacaagcctttggactaacgggaBstXICas91,9401,9601,9802,0002,0202,0402,0602,0802,100
gagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgctcggacccggactgggggttgaagttctcgttgaagctggaccggctcctacggtttgacgtcgactcgttcctgtggatgctgctgctggacctgttggacgaccgggtctagccgctggtcatgcggctggacaaagaccggcggttcttggacaggctgcggtaggacgacEcoNICas92,1202,1402,1602,1802,2002,2202,2402,260
agcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccgtcgctgtaggactctcacttgtggctctagtggttccggggggactcgcggagatactagttctctatgctgctcgtggtggtcctggactgggacgactttcgagagcacgccgtcgtcgacggactcttcatgtttctctaaaagaagctggtctcgttcttgccgatgcggcCas92,2802,3002,3202,3402,3602,3802,4002,4202,440
gctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctcgatgtaactgccgcctcggtcggtccttctcaagatgttcaagtagttcgggtaggaccttttctacctgccgtggctccttgacgagcacttcgacttgtctctcctggacgacgccttcgtcgcctggaagctgttgccgtcgtagggggtggtctaggtggaccctctcgaCas92,4602,4802,5002,5202,5402,5602,5802,6002,620
gcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaaccgtgcggtaagacgccgccgtccttctaaaaatgggtaaggacttcctgttggcccttttctagctcttctaggactggaaggcgtaggggatgatgcacccgggagaccggtcccctttgtcgtctaagcggacctactggtctttctcgctcctttggtagtgggggaccttgApaIPspOMICas92,6402,6602,6802,7002,7202,7402,7602,7802,800
ttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaaagctccttcaccacctgttcccgcgaaggcgggtctcgaagtagctcgcctactggttgaagctattcttggacgggttgctcttccacgacgggttcgtgtcggacgacatgctcatgaagtggcacatattgctcgactggtttcactttatgcactggctcccttactcttBarICas92,8202,8402,8602,8802,9002,9202,9402,960
agcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacatatcgggcggaaggactcgccgctcgtctttttccggtagcacctggacgacaagttctggttggcctttcactggcacttcgtcgactttctcctgatgaagttcttttagctcacgaagctgaggcacctttagaggccgcaccttctagccaagttgcggagggacccgtgtatPasICas92,9803,0003,0203,0403,0603,0803,1003,1203,140
ccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagggtgctagacgacttttaatagttcctgttcctgaaggacctgttactccttttgctcctgtaagaccttctatagcacgactgggactgtgacaaactcctgtctctctactagctccttgccgacttttggatacgggtggacaagctgctgtttcactacttcgtcgacttcEcoRVCas93,1603,1803,2003,2203,2403,2603,2803,3003,320
cggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaaggccgcctctatgtggccgaccccgtccgactcggccttcgactagttgccgtaggccctgttcgtcaggccgttctgttaggacctaaaggacttcaggctgccgaagcggttgtctttgaagtacgtcgactaggtgctgctgtcggactggaaatttctcctgtaggtctttcAjuICas93,3403,3603,3803,4003,4203,4403,4603,4803,500
cccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagacgggtccacaggccggtcccgctatcggacgtgctcgtgtaacggttagaccggccgtcggggcggtaattcttcccgtaggacgtctgtcacttccaccacctgctcgagcactttcactacccggccgtgttcgggctcttgtagcactagctttaccggtctctcttggtctgSacIEco53kICas93,5203,5403,5603,5803,6003,6203,6403,660
cacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaagtgggtcttccctgtcttcttgtcggcgctctcttacttcgcctagcttctcccgtagtttctcgacccgtcggtctaggactttcttgtggggcaccttttgtgggtcgacgtcttgctcttcgacatggacatgatggacgtcttacccgccctatacatgcacctggtccttCas93,6803,7003,7203,7403,7603,7803,8003,8203,840
ctggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagcgacctgtagttggccgacaggctgatgctacacctggtatagcacggagtctcgaaagacttcctgctgaggtagctgttgttccacgactggtcttcgctgttcttggccccgttctcgctgttgcacgggaggcttctccagcacttcttctacttcttgatgaccgccgtcgPflMICas93,8603,8803,9003,9203,9403,9603,9804,0004,020
tgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgaacgacttgcggttcgactaatgggtctctttcaagctgttagactggttccggctctctccgccggactcgcttgacctattccggccgaagtagttctctgtcgaccacctttgggccgtctagtgtttcgtgcaccgtgtctaggacctgagggcctacttgtgattcatgctPmlICspCICas94,0404,0604,0804,1004,1204,1404,1604,1804,200
cgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctgctcttactgttcgactaggcccttcactttcactagtgggacttcaggttcgaccacaggctaaaggccttcctaaaggtcaaaatgtttcacgcgctctagttgttgatggtggtgcgggtgctgcggatggacttgcggcagcacccttggcgggactagtttttcatgggaCas94,2204,2404,2604,2804,3004,3204,3404,360
aagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcttcgacctttcgctcaagcacatgccgctgatgttccacatgctgcacgccttctactagcggttctcgctcgtcctttagccgttccgatggcggttcatgaagaagatgtcgttgtagtacttgaaaaagttctggctctaatgggaccggttgccgctctaggccttcgccgAjuIBmgBICas94,3804,4004,4204,4404,4604,4804,5004,5204,540
ctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgagactagctctgtttgccgctttggcccctctagcacaccctattcccggccctaaaacggtggcacgcctttcacgactcgtacggggttcacttatagcactttttctggctccacgtctgtccgccgaagtcgtttctcagataggacgggttctccttgtcgctattcgaSphICas94,5604,5804,6004,6204,6404,6604,6804,7004,720
gatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatctagcggtctttcttcctgaccctgggattcttcatgccgccgaagctgtcggggtggcaccggataagacacgaccaccaccggtttcaccttttcccgttcaggttctttgacttctcacactttctcgacgacccctagtggtagtacctttcttcgtcgaagctcttcttaBarIKflIBsaBICas94,7404,7604,7804,8004,8204,8404,8604,8804,900
cccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgccctccaaatatgtgaacttccgggtagctgaaagaccttcggttcccgatgtttcttcactttttcctggactagtagttcgacggattcatgagggacaagctcgaccttttgccggccttctcttacgaccggagacggccgcttgacgtcttccctttgcttgaccgggacgggaggtttatacacttgaaggBsmIXcmICas94,9204,9404,9604,9805,0005,0205,0405,060
tgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctaacatggaccggtcggtgatactcttcgacttcccgagggggctcctattactcgtctttgtcgacaaacaccttgtcgtgttcgtgatggacctgctctagtagctcgtctagtcgctcaagaggttctctcactaggaccggctgcgattagacctgtttcacgacaggcggatCas95,0805,1005,1205,1405,1605,1805,2005,2205,240
caacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagcatcgttgttcgtggccctattcgggtagtctctcgtccggctcttatagtaggtggacaaatgggactggttagaccctcggggacggcggaagttcatgaaactgtggtggtagctggccttctccatgtggtcgtggtttctccacgacctgcggtgggactaggtggtctcgtagXcmICas95,2605,2805,3005,3205,3405,3605,3805,4005,420
accggcctgtacgagacacggatcgacctgtctcagctgggaggcgacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaaggaattcggcagtggagagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaatgaccgagttggccggacatgctctgtgcctagctggacagagtcgaccctccgctgttttccggccgccggtgctttttccggccggtccgttttttctttttccttaagccgtcacctctcccgtctccttcagacgattgtacgccactgcagctcctcttaggaccgggttactggctcaFseIBseRIEcoRIT2ACas9nucleoplasmin NLSPu...RPuroR5,4405,4605,4805,5005,5205,5405,5605,5805,600
acaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggtgttcgggtgccacgcggagcggtgggcgctgctgcaggggtcccggcatgcgtgggagcggcggcgcaagcggctgatggggcggtgcgcggtgtggcagctaggcctggcggtgtagctcgcccagtggctcgacgttcttgagaaggagtgcgcgcagcccgagctgtagccPasIBsiWIPflFIRsrIIBstEIITth111IPuroRPu...R5,6205,6405,6605,6805,7005,7205,7405,760
caaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggaggttccacacccagcgcctgctgccgcggcgccaccgccagacctggtgcggcctctcgcagcttcgcccccgccacaagcggctctagccgggcgcgtaccggctcaactcgccaagggccgaccggcgcgtcgttgtctaccttccggaggaccgcggcgtggccgggttcctcStuISacIIPuroR5,7805,8005,8205,8405,8605,8805,9005,9205,940
cccgcgtggttcctggccaccgtcggagtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcagggcgcaccaaggaccggtggcagcctcagagcgggctggtggtcccgttcccagacccgtcgcggcagcacgaggggcctcacctccgccggctcgcgcggccccacgggcggaaggacctctggaggcgcggggcgttggaggggaagatgctcgccgagccgaagtggcagtBsaIPuroRPuro-F5,9605,9806,0006,0206,0406,0606,0806,1006,120
ccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgagaattctaactagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccggcggctgcagctccacgggcttcctggcgcgtggaccacgtactgggcgttcgggccacggactcttaagattgatctcgagcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggSacIEcoRISexAIEco53kIbGH poly(A) signalPuroRBGH-rev6,1406,1606,1806,2006,2206,2406,2606,2806,300
actgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggagcggccgcaggaacccctagtgatggagttggccactccctctctgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctcttatcgtccgtacgacccctcgccggcgtccttggggatcactacctcaaccggtgagggagagNotISphIbGH poly(A) signalAAV2 ITRAAV2 ITR6,3206,3406,3606,3806,4006,4206,4406,460
tgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccaacgcgcgagcgagcgagtgactccggcccgctggtttccagcgggctgcgggcccgaaacgggcccgccggagtcactcgctcgctcgcgcgtcgacggacgtccccgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatgcagtttcgttggtSbfISmaISmaISrfISrfIXmaIXmaITspMITspMIAAV2 ITRAAV2 ITR6,4806,5006,5206,5406,5606,5806,6006,6206,640
tagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcggaatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaf1 oriF1ori-R6,6606,6806,7006,7206,7406,7606,7806,8006,820
tccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgagatagagcccgatf1 oriF1ori-F6,8406,8606,8806,9006,9206,9406,9606,9807,000
ttcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaaagaaaactaaatattccctaaaacggctaaagccagataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaaataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcggggctgtgggcggtPsiISspIf1 oripRS-marker7,0207,0407,0607,0807,1007,1207,1407,160
acacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctccaaaagtggcagtagtggctttgcgcgctctgctttcccggagcactatgcggataaaaatatccaattacagtactattattBsmBIBsmBIEsp3IEsp3IpGEX 3'pBRforEco7,1807,2007,2207,2407,2607,2807,3007,3207,340
tggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctaccaaagaatctgcagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaSspIAmpR promoterAmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
tattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgAmpR7,5407,5607,5807,6007,6207,6407,6607,6807,700
acttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtAmpRAmp-R7,7207,7407,7607,7807,8007,8207,8407,860
tgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacatcgttaccgPvuIAmpR7,8807,9007,9207,9407,9607,9808,0008,0208,040
aacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagccgcggtatcttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcaccttcggcgccatagFspISacIIAmpR8,0608,0808,1008,1208,1408,1608,1808,2008,220
attgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaataacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattAmpR8,2408,2608,2808,3008,3208,3408,3608,3808,400
aacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaattgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgttttttori8,4208,4408,4608,4808,5008,5208,5408,560
accaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatctggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattagori8,5808,6008,6208,6408,6608,6808,7008,7208,740
ctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactcori8,7608,7808,8008,8208,8408,8608,8808,9008,920
aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggtttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtcccccoripBR322ori-F8,9408,9608,9809,0009,0209,0409,0609,0809,100
gcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtcgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaPciIAflIIIori9,1209,1409,160
  • Plasmid
Editing disabled on read-only sequence.
9177 bp65007000750080008500500100015002000Cas93xFLAGConfirmed by seq

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9177