Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
working...
Advanced Settings
plRL125 KAE
  • Sequence Map
Editing disabled on read-only sequence.
CACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGATCGATTTAatGGGTCTGACGCTCATGGAACAATAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTATCTGTTGTTTGAAAAAAAAAAGCCACGCGATTGCGGGGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACTAGCTAAATtaCCCAGACTGCGAGTACCTTGTTATTTGCTTTCCGAGTCAGCTTTCTGACCCGGAAAGCAAATAGACAACAAACTTTTTTTTTTCGGTGCGCTAACGCCCCClaIAlwNIBspDIAmplicon plJR876insertrnBT1 (hairpin -15.8 kcal/mol)TsynA (hai...kcal/mol)3292182520406080100120140160
CTTTTTTTTTTCATATGtatcaCTTAAGattcaaatactagtgttaactatttaattggggaccctagaggtccccttttttattttaaattaattagctaaagcgctagcccatggtcaTGGAAACCGTTGTGGTCTCCCTATAGTGAGTCGTATTAATTTACGCGTTTCACTGGAAAAAAAAAAGTATACatagtGAATTCtaagtttatgatcacaattgataaattaacccctgggatctccaggggaaaaaataaaatttaattaatcgatttcgcgatcgggtaccagtACCTTTGGCAACACCAGAGGGATATCACTCAGCATAATTAAATGCGCAAAGTGACAfeIBmtIBsaIDraIHpaIKflIMluIMlyINcoINdeINheIPleISpeIStyIAflIIBcoDIBsmAIBstXIHaeIIPpuMIPpuMIEcoO109IEcoO109IAmplicon plJR962qtag19T7 promoterAmplicon plJR876T4_32 terminsertTs...)180200220240260280300320340
GCCGACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCGGCTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGADrdINspIPciIoriE360380400420440460480500520
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCcTCGGGAAGCGTGGCGCTTTCTCAtaGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGgAGCCCTTCGCACCGCGAAAGAGTatCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACApaLIBciVIBseYIBssSIHaeIIBssSαIoriE540560580600620640660680700
CGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGMlyIPleIAlwNIoriE720740760780800820840860
GCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACTCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGAAcuISpeIoriE8809009209409609801,0001,0201,040
AGTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAGATGCATTCAGTCCTGCTCCTCGGTCAGCGTTGCAAGTTTAGGCGAGGGCCGCCTAAACAGGATGAGTCCTCTCGCAAGTGGCTGTTTGTTGTCTATTTTGCTTTCCGGGTCAGAAAGCTGACTCGGAAAGCAAAATAAACTACGGACCGTCAAGGGATGAGAGCGTACCCCTCTCTACGTAAGTCAGGACGAGGAGCCAclIBsmINsiIBseRIBsrBINmeAIIITrrnBrrnBble (zeoR)1,0601,0801,1001,1201,1401,1601,1801,2001,220
CCACGAAGTGCACGCAGTTGCCGGCCGGGTCGCGCAGGGCGAACTCCCGCCCCCACGGCTGCTCGCCGATCTCGGTCATGGCCGGCCCGGAGGCGTCCCGGAAGTTCGTGGACACGACCTCCGACCACTCGGCGTACAGCTCGTCCAGGCCGCGCACCCACACCCAGGCCAGGGTGGTGCTTCACGTGCGTCAACGGCCGGCCCAGCGCGTCCCGCTTGAGGGCGGGGGTGCCGACGAGCGGCTAGAGCCAGTACCGGCCGGGCCTCCGCAGGGCCTTCAAGCACCTGTGCTGGAGGCTGGTGAGCCGCATGTCGAGCAGGTCCGGCGCGTGGGTGTGGGTCCGGTCCCABglIFseIApaLIBsaXIDraIIINmeAIIIble (zeoR)1,2401,2601,2801,3001,3201,3401,3601,3801,400
GTTGTCCGGCACCACCTGGTCCTGGACCGCGCTGATGAACAGGGTCACGTCGTCCCGGACCACACCGGCGAAGTCGTCCTCCACGAAGTCCCGGGAGAACCCGAGCCGGTCGGTCCAGAACTCGACCGCTCCGGCGACGTCGCGCGCGGTGAGCACCGGAACGGCACTGGTCAACCAACAGGCCGTGGTGGACCAGGACCTGGCGCGACTACTTGTCCCAGTGCAGCAGGGCCTGGTGTGGCCGCTTCAGCAGGAGGTGCTTCAGGGCCCTCTTGGGCTCGGCCAGCCAGGTCTTGAGCTGGCGAGGCCGCTGCAGCGCGCGCCACTCGTGGCCTTGCCGTGACCAGTTGBanISmaIXmaIZraIAatIIBsrBIMauBISexAISgrAITspMIBssHIIble (zeoR)1,4201,4401,4601,4801,5001,5201,5401,560
TTGGCCATGGTTTAGTTCCTCACCTTGTCGTATTATACTATGCCGATATACTATGCCGATGATTAATTGTCAACACCTGCAGGTCGAGATTTACAGTCGGTCGCCTCCGGCCGACGGCGGTGCGGGCCCGGCCGGCCGCGATCGCCGCCGACCTACACGTATTCGCAGAGTTAAGAACCGGTACCAAATCAAGGAGTGGAACAGCATAATATGATACGGCTATATGATACGGCTACTAATTAACAGTTGTGGACGTCCAGCTCTAAATGTCAGCCAGCGGAGGCCGGCTGCCGCCACGCCCGGGCCGGCCGGCGCTAGCGGCGGCTGGATGTGCATAAGCGTCTCAATTCApaIBcgIBglIFseIMscINcoIPstIPvuISbfISfiIStyIAsiSIBanIIBsaAIPaqCIPspOMIEM7 promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
CGGTCACCCCCGCCGGTTTGACCCCAATTTTGACCCCAATTGCATCGGGGTTCGGGGCGATTCGCCGATGTCCGCCATTGTGCGCAACCGGGCGTGACCTGCGGATTCGGTACCCGGGCGGATCCACGCGGATGCCTTTTGAATGACTGGGGGTCAAGGGGTCGCAGGTTCAAATGCCAGTGGGGGCGGCCAAACTGGGGTTAAAACTGGGGTTAACGTAGCCCCAAGCCCCGCTAAGCGGCTACAGGCGGTAACACGCGTTGGCCCGCACTGGACGCCTAAGCCATGGGCCCGCCTAGGTGCGCCTACGGAAAACTTACTGACCCCCAGTTCCCCAGCGTCCAAGTTTAAhdIBanIFokIFspIKpnIMfeISmaIXmaIBamHIBtsCITspMIAcc65IBstEIIattPattP1,7601,7801,8001,8201,8401,8601,8801,9001,920
CCTGTCAGCCCGACGCAGATCAGGGGCGGTTTTCCGGAGACGGGAAGCCGCCCCTTTTTCTGTTTGACCCCAATTTTGACCCCAATTGCTCCGGCGTCCGTCCGCGTCCGTCGAGCTCGCTAAGACTGTCCGCTGCCGTCCGCGATCCGCAGGCGCGCCGACAACACGTCGACCAGGACAGTCGGGCTGCGTCTAGTCCCCGCCAAAAGGCCTCTGCCCTTCGGCGGGGAAAAAGACAAACTGGGGTTAAAACTGGGGTTAACGAGGCCGCAGGCAGGCGCAGGCAGCTCGAGCGATTCTGACAGGCGACGGCAGGCGCTAGGCGTCCGCGCGGCTGTTGTGCAGCTGGTAccIAhdIAscIMfeISacISalIBanIIBcoDIBsmAIBsmBIBspEIBsrDIEsp3IBssHIIEco53kIattPattP'int-giles1,9401,9601,9802,0002,0202,0402,0602,0802,100
TTGCGGTGTCCTCTCGGGGGTCGCCGGGTGCGTGGGGATCCACGCCGAATAGTGCCCGGTCGGGATAGGTGTACGGTCGCCTCCGGTCACGTCCGGGCGTCCGCGTACGCCGATTGTGTCACCGCGGCGATCATCATTCCAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTAACGCCACAGGAGAGCCCCCAGCGGCCCACGCACCCCTAGGTGCGGCTTATCACGGGCCAGCCCTATCCACATGCCAGCGGAGGCCAGTGCAGGCCCGCAGGCGCATGCGGCTAACACAGTGGCGCCGCTAGTAGTAAGGTTTTCCTAGATCCACTTCTAGGAAAAACTATTAGABamHIBsiWISacII2,1202,1402,1602,1802,2002,2202,2402,260
CATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAABspHI2,2802,3002,3202,3402,3602,3802,4002,4202,440
TTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGAcuI2,4602,4802,5002,520
  • Plasmid
Editing disabled on read-only sequence.
2525 bp175020002250250500oriEattPattPT7 promoterAmplicon plJR9623292olKE1416 (DNA)olKE1418 (DNA)complementation FWD (DNA)olKE1421 (DNA)olKE1417 (DNA)olJR1835 (DNA)

Welcome to Benchling!

Sign in to view data.

New digest

Saved digests

Enzyme AatII
Enzyme lists
Cut sites visible on maps
Find enzyme
Name Cuts
Selected Color
Show enzymes that cut

Primers

Version history

 Show full history
Change User Timestamp

Sequence Alignments

Saved alignments

Sequence Info

Tags

Name
Parent version
Folder
Topology
Start index
Locked
Created
--
Length
--
Export data
Split Workspace
Bases
2525