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XZ033 CMV-TO-CCL20-pre-mGRASP (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcCMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaNcoICspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcSacISacIBsaBIBsaBISgrDIEco53kIEco53kICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccATGTGCTGTACCAAGAGTTTGCTCCTGGCTGCTTTGATGTCAGTGCTGCTACTCCACCTCTGCGGCGAATCAGAAGCAGCAAGCAACTTTGACTGCTGTCTTGGATACACAGACCGttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggTACACGACATGGTTCTCAAACGAGGACCGACGAAACTACAGTCACGACGATGAGGTGGAGACGCCGCTTAGTCTTCGTCGTTCGTTGAAACTGACGACAGAACCTATGTGTCTGGCPmeIPshAIHindIIIMC2CT4KS6LL8LA10AL12MS14VL16LL18HL20CG22ES24EA26AS28NF30DC32CL34GY36TD38R760-2079Ccl20Translation 1731-3050CMV promot...th TetOs)homolog720740760780800820840860
    TATTCTTCATCCTAAATTTATTGTGGGCTTCACACGGCAGCTGGCCAATGAAGGCTGTGACATCAATGCTATCATCTTTCACACAAAGAAAAAGTTGTCTGTGTGCGCAAATCCAAAACAGACTTGGGTGAAATATATTGTGCGTCTCCTCAGTAAAAAAGTCAAGAACATGGGTATAAGAAGTAGGATTTAAATAACACCCGAAGTGTGCCGTCGACCGGTTACTTCCGACACTGTAGTTACGATAGTAGAAAGTGTGTTTCTTTTTCAACAGACACACGCGTTTAGGTTTTGTCTGAACCCACTTTATATAACACGCAGAGGAGTCATTTTTTCAGTTCTTGTACCCABaeIFspIMscIXcmIAcc65II40LH42PK44FI46VG48FT50RQ52LA54NE56GC58DI60NA62II64FH66TK68KK70LS72VC74AN76PK78QT80WV82KY84IV86RL88LS90KK92VK94NM96G760-2079Translation 1731-3050Ccl208809009209409609801,0001,0201,040
    ACCGGGAACGGAAATGGCAATGGTAACGGGAATGGAAACGGCAATGGTAACGGGAATACTAGTGTTAACACCGGTGCGGCCGTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTCTGGCCCTTGCCTTTACCGTTACCATTGCCCTTACCTTTGCCGTTACCATTGCCCTTATGATCACAATTGTGGCCACGCCGGCAAATGGGTATGCTACAAGGACTGATACGCCCGATAGGGATACTGCAGGGCCTGATACGTCCTAGGATAGGTATACTGCAAGGTCTAATGCGAGAgeIHpaIKpnISpeIBamHISgrAIT98GN100GN102GN104GN106GN108GN110GN112GN114GN116TS118VN120TG122AA124VY126PY128DV130PD132YA134GY136PY138DV140PD142YA144GS146YP148YD150VP152DY154A760-2079Translation 1731-30503xHAflexible linker1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CGGCCGCCACCGGTCTTAAGGTCGACTTTCAGAAAGCTAGCTCTATTGTTTATAAGAAGGAGGGGGAACAGGTGGAGTTCAGTTTCCCATTGGCCTTCACAGTCGAGAAGCTCACAGGATCAGGAGAGTTGTGGTGGCAGGCCGAAAGAGCCTCCTCCAGCAAGAGTTGGATCACGCCGGCGGTGGCCAGAATTCCAGCTGAAAGTCTTTCGATCGAGATAACAAATATTCTTCCTCCCCCTTGTCCACCTCAAGTCAAAGGGTAACCGGAAGTGTCAGCTCTTCGAGTGTCCTAGTCCTCTCAACACCACCGTCCGGCTTTCTCGGAGGAGGTCGTTCTCAACCTAGTGAgeIBmtINheIPsiIP156AA158TG160LK162VD164FQ166KA168SS170IV172YK174KE176GE178QV180EF182SF184PL186AF188TV190EK192LT194GS196GE198LW200WQ202AE204RA206SS208SK210SW212IT214760-2079Translation 1731-3050CD4pre mgrasp1,2401,2601,2801,3001,3201,3401,3601,3801,400
    TTTTGATCTGAAAAACAAAGAAGTGAGCGTGAAGAGAGTCACACAGGACCCTAAGCTGCAGATGGGCAAGAAGTTGCCTTTGCACTTGACACTCCCCCAAGCCCTGCCCCAGTATGCTGGCTCCGGGAACCTCACCCTCGCCTTGGAGGCTAAGACCGGAAAGTTGCATCAGGAGAAAACTAGACTTTTTGTTTCTTCACTCGCACTTCTCTCAGTGTGTCCTGGGATTCGACGTCTACCCGTTCTTCAACGGAAACGTGAACTGTGAGGGGGTTCGGGACGGGGTCATACGACCGAGGCCCTTGGAGTGGGAGCGGAACCTCCGATTCTGGCCTTTCAACGTAGTCCTCBstXIBpu10IFD216LK218NK220EV222SV224KR226VT228QD230PK232LQ234MG236KK238LP240LH242LT244LP246QA248LP250QY252AG254SG256NL258TL260AL262EA264KT266GK268LH270QE272760-2079Translation 1731-3050CD4pre mgrasp1,4201,4401,4601,4801,5001,5201,5401,560
    GTCAACCTCGTGGTGATGAGGGCAACACAGCTGCAGAAGAACCTGACCTGCGAAGTGTGGGGTCCCACCTCACCTAAACTGATGCTGTCTCTGAAGCTGGAGAATAAGGAGGCTAAAGTCAGTAAAAGAGAGAAGGCTGTGTGGGTCCTTAACCCGGAGGCAGGGATGTGGCAGTCAGTTGGAGCACCACTACTCCCGTTGTGTCGACGTCTTCTTGGACTGGACGCTTCACACCCCAGGGTGGAGTGGATTTGACTACGACAGAGACTTCGACCTCTTATTCCTCCGATTTCAGTCATTTTCTCTCTTCCGACACACCCAGGAATTGGGCCTCCGTCCCTACACCGTCAKflIBfuAIBspMIVN274LV276VM278RA280TQ282LQ284KN286LT288CE290VW292GP294TS296PK298LM300LS302LK304LE306NK308EA310KV312SK314RE316KA318VW320VL322NP324EA326GM328WQ330760-2079Translation 1731-3050CD4pre mgrasp1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GTCTTCTTAGCGACTCTGGACAGGTGCTGCTGGAGAGCAACATCAAGGTGCTTCCTACTTGGTCAACACCAGTACAGCCGATGGCCCTGATCGTACTCGGGGGAGTCGCCGGCCTGCTTCTGTTTATCGGCCTGGGCATCTTCTTCTGCGTACGCTACAAGTATCGCAACAGGGACAGAAGAATCGCTGAGACCTGTCCACGACGACCTCTCGTTGTAGTTCCACGAAGGATGAACCAGTTGTGGTCATGTCGGCTACCGGGACTAGCATGAGCCCCCTCAGCGGCCGGACGAAGACAAATAGCCGGACCCGTAGAAGAAGACGCATGCGATGTTCATAGCGTTGTCCCTAvaIBsiWIBsoBICL332LS334DS336GQ338VL340LE342SN344IK346VL348PT350WS352TP354VQ356PM358AL360IV362LG364GV366AG368LL370LF372IG374LG376IF378FC380VR382YK384YR386NR388D760-2079Translation 1731-3050pre mgraspCD4NXN cyt...region1,7601,7801,8001,8201,8401,8601,8801,9001,920
    TGAGGGATCTTACCACGTTGACGAATCTAGGAACTATATCAGTAACAGCGCTCAGAGTAATGGCGCCGTGGTTAAAGAGAAACAGCCCTCCAGCGCCAAATCAGCAAACAAAAACAAGAAAAACAAGGATAAAGAGTACTACGTACTTAAGtaagccgctcgaggagggcccgaaACTCCCTAGAATGGTGCAACTGCTTAGATCCTTGATATAGTCATTGTCGCGAGTCTCATTACCGCGGCACCAATTTCTCTTTGTCGGGAGGTCGCGGTTTAGTCGTTTGTTTTTGTTCTTTTTGTTCCTATTTCTCATGATGCATGAATTCattcggcgagctcctcccgggcttAfeIApaIAvaIKasINarISfoITliIXhoIBsoBIPluTIPspXISnaBIPaeR7IPspOMIE390GS392YH394VD396ES398RN400YI402SN404SA406QS408NG410AV412VK414EK416QP418SS420AK422SA424NK426NK428KN430KD432KE434YY436VL438K*440760-2079Translation 1731-3050pre mgraspNXN cytoplasmic regionhomolog1,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaBclIPmeIBGH polyadenylation sequencec-myc epitopePolyhi...e tag2,1202,1402,1602,1802,2002,2202,2402,260
    cctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataClaINspISphIBspDIBGH polyadenylation sequenceBGH pA2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcgg2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggactBarIKflIMscIXbaIFRTHy...n2,6402,6602,6802,7002,7202,7402,7602,7802,800
    actcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaBsaXIPflFIPshAITth111IHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    tatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctNcoIBfuAIBspMIEcoRIBstAPIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgaagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactPvuIAsiSIPflFIRsrIITth111IHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccBstAPINmeAIIIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcAjuISacIIHygromycin3,5203,5403,5603,5803,6003,6203,6403,660
    tccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctaaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatHygromycin3,6803,7003,7203,7403,7603,7803,8003,8203,840
    tgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaBsmIPsiISV40 pA3,8603,8803,9003,9203,9403,9603,9804,0004,020
    tctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacBstZ17ISV40 pA4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcgggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagcSapIBsaXIBspQI4,2204,2404,2604,2804,3004,3204,3404,360
    gctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaacgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttNspIPciIAflIIIpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccapUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    atctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgttagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacapUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagtccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtcpUC ori4,9204,9404,9604,9805,0005,0205,0405,060
    attacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatattaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatataBspHIpUC ori5,0805,1005,1205,1405,1605,1805,2005,2205,240
    atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctctactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagAhdIBsaIAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    accggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtgtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcacAclIFspINmeAIIIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcatacacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtatPvuIAmp (bla)5,6205,6405,6605,6805,7005,7205,7405,760
    attctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttctaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagAclIBcgIXmnIAmp (bla)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaactAmp (bla)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatgtatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggatacSspIBspHISgrDIbla promoter6,1406,1606,1806,2006,2206,2406,2606,2806,300
    gtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaaMfeIBpu10I6,3206,3406,3606,3806,4006,4206,4406,460
    cgtacgggccagatatacgcgcatgcccggtctatatgcgMluIBsiWIAflIII6,480
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6495 bp450050005500600050010001500pre mgraspCD4TetO760-2079EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.4 (DNA)EKo272.1 (DNA)EK_CMVFa (DNA),EK_CMVFb (DNA),EK_CMVFc (DNA),SpeICMV,CMV promoter (with TetOs),A,EK_CMVFd (DNA),EK_CMVFe (DNA),SnaBI,NcoI,+1TATA box,TetO,TetR binding sites,CMV-Forward Genewiz (DNA),Eco53kI,+3homolog,CMVF1 (DNA),ToFlpF (DNA),SgrDI,Eco53kI,SacI,PmeIKozak,XZp033.1 (DNA),HindIIICcl20,PshAI,MscIFspI,XcmIflexible linker,BaeI,Acc65I,KpnIHpaI,AgeI,3xHA,mCCL20-seq-F1 (DNA),SpeI,HpaI,AgeI,SgrAIAgeI,AflII,CD4,pre mgrasp,BamHI,AgeI,NheIBmtIPsiITranslation 1731-3050,Bpu10IBstXImCCL20-seq-F2 (DNA)BspHIAhdIEKo152.2 (DNA),BsaINmeAIIIFspI,AclIAmp (bla),PvuIBcgIAclI,XmnIbla promoter,SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),FlpInF2 (DNA),EKo279.1 (DNA),SgrDI,MfeI,Bpu10I,BsiWI,+2

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    6495