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pOMRP3
  • Sequence Map
    Editing disabled on read-only sequence.
    taactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcAlwNIBB seq FORsourceori20406080100120140160
    ccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaggcctactattagcaacaacgatcctttgatcttttctacggggtctgacgctcaggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttccggatgataatcgttgttgctaggaaactagaaaagatgccccagactgcgagtStuIupstream annealdownstream anneal20bp ra...quencesourceori180200220240260280300320340
    gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacaBanIAmpRpOpen ori anneal REVBB seq REVsource360380400420440460480500520
    ctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaaccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctggataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctcttggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacAhdIBglIAmpRsource540560580600620640660680700
    caactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgAseIFspINmeAIIIAmpRsource720740760780800820840860
    atgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtBtsIBtsIPvuIScaIBtsαIBtsαIAmpRsource8809009209409609801,0001,0201,040
    ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacXmnIApaLIBssSIBssSαIAmpRsource1,0601,0801,1001,1201,1401,1601,1801,2001,220
    cacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgggtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgccEarISspIAmpRAmpR promotersource1,2401,2601,2801,3001,3201,3401,3601,3801,400
    atacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacctgcaccagtcagtaaaacgacggccagtagtcaaaagcctccgaccggaggcttttgacttggtcaggtggagtgggagtagtcttcgccatcgcAGAGGCGTCGTCAACGCCCTCTCCCGATtatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtggacgtggtcagtcattttgctgccggtcatcagttttcggaggctggcctccgaaaactgaaccagtccacctcaccctcatcagaagcggtagcgTCTCCGCAGCAGTTGCGGGAGAGGGCTAPaqCIPflFITth111IAmpR promoterVector.FORtonB terminatorG3PDp_10597SS1G3PD_10597SS1Fragment.FORSLiCE-revM13 fwdAarIsource1,4201,4401,4601,4801,5001,5201,5401,560
    GAGATTGTGCGGGTTGACTTGGAGAGCGGGAGGGAGAGTCCGACGGGGACGGGGGTGAGGTGACACTTCGGTCCGGGGGTGAATGTCGCAGGAGCGATGACATCATGATGTGAATATCAGTCGGCAGGGCATGGCAAGTGCGTGTGTTATACTTGGTGTCTGTGCGGCAACCAGTCTCTAACACGCCCAACTGAACCTCTCGCCCTCCCTCTCAGGCTGCCCCTGCCCCCACTCCACTGTGAAGCCAGGCCCCCACTTACAGCGTCCTCGCTACTGTAGTACTACACTTATAGTCAGCCGTCCCGTACCGTTCACGCACACAATATGAACCACAGACACGCCGTTGGTCARsrIIG3PDp_10597SS1G3PD_10597SS1Core_putativesource1,5801,6001,6201,6401,6601,6801,7001,7201,740
    GGAAGCTGACGACTGATGATGGTGTTTCAGATTTGGGTCCAGCCCGTGCCCTGTCATCCGAGGACCTGCGAATACTTCAACCTCATAGGTCGAATCCATGAGGACTGATGACAGGAGGTTTCCCGGATTTTGCGATTTGATGTGCCTCGGGTGGCACTCAGACACTCTAACGCTTCCTTCGACTGCTGACTACTACCACAAAGTCTAAACCCAGGTCGGGCACGGGACAGTAGGCTCCTGGACGCTTATGAAGTTGGAGTATCCAGCTTAGGTACTCCTGACTACTGTCCTCCAAAGGGCCTAAAACGCTAAACTACACGGAGCCCACCGTGAGTCTGTGAGATTGCGAANmeAIIIG3PDp_10597SS1G3PD_10597SS1source1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ATTCTCGGCCAGTGCTTTTATCACTATGTAATGTGTGCGTTGTGTGTTGCCTGACTTGTTGTACAACCAGTATAGACTTCATCGATGTATGAACCAGGGTGTGACTATTCGCTTGGCTGGGCTCTTACTCTTTCTCAGTGGCAAGGTGCCTTCAAGAGCGGTTCCTTGCGGAGCATAAGAGCCGGTCACGAAAATAGTGATACATTACACACGCAACACACAACGGACTGAACAACATGTTGGTCATATCTGAAGTAGCTACATACTTGGTCCCACACTGATAAGCGAACCGACCCGAGAATGAGAAAGAGTCACCGTTCCACGGAAGTTCTCGCCAAGGAACGCCTCGTBanIBsrGIG3PDp_10597SS1G3PD_10597SS1source1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CCACCTGAGCGGCCCTGCCCGGGTTCAGGTTGAGCTTGCAGGCTAAACATCGATGTAGGGTTAATCAGATGTAGAATGCGGTCGCCTTCTGCCTTGCCCGTAGTGCAGGACTACGACTGCAGCACCCAACACGACTGTGGAATGGCGAGCAGCACAGGGCAGCTTCTAACCCATGGGTGGACTCGCCGGGACGGGCCCAAGTCCAACTCGAACGTCCGATTTGTAGCTACATCCCAATTAGTCTACATCTTACGCCAGCGGAAGACGGAACGGGCATCACGTCCTGATGCTGACGTCGTGGGTTGTGCTGACACCTTACCGCTCGTCGTGTCCCGTCGAAGATTGGGTACAleIBtgINcoIPstISmaIStyIXcmIXmaITspMIG3PDp_10597SS1G3PD_10597SS1source2,1202,1402,1602,1802,2002,2202,2402,260
    GAGAGACAAGGGGATGGGCGCGCCAGTAAGCGCATATGCCATTATGGTCCACTGAGACTGACCGGTGTATCCACCAGGCTGTCTTTGTTGACAGATCTACCCGAACGCTCTCTGTGCGCCGGAGTCGGCGGCCGGCACGGAGCACTGGGCCGATTCCGTCAATATGACAAACCTCCTCTCTGTTCCCCTACCCGCGCGGTCATTCGCGTATACGGTAATACCAGGTGACTCTGACTGGCCACATAGGTGGTCCGACAGAAACAACTGTCTAGATGGGCTTGCGAGAGACACGCGGCCTCAGCCGCCGGCCGTGCCTCGTGACCCGGCTAAGGCAGTTATACTGTTTGGAGAgeIAscIBaeIEagINaeINdeIBglIIBssHIINgoMIVG3PDp_10597SS1G3PD_10597SS1source2,2802,3002,3202,3402,3602,3802,4002,4202,440
    CTGCAATCGAGTCATGCACTGTGTGCTGTGCATTGCGATCGAATCGGAGGCGTCTTCTCTTGTGTGCCTATAGGTTTGGTGAGTTTCCCTGTTCCAACGTAACATTCGTTTGTATTGCCTTCTGACCAAGCACCCAGAAGGAGGGGGAGGGGGAGGATGACATTTCGGCGTGTATGACGTTAGCTCAGTACGTGACACACGACACGTAACGCTAGCTTAGCCTCCGCAGAAGAGAACACACGGATATCCAAACCACTCAAAGGGACAAGGTTGCATTGTAAGCAAACATAACGGAAGACTGGTTCGTGGGTCTTCCTCCCCCTCCCCCTCCTACTGTAAAGCCGCACATAPvuIBstAPIDraIIIG3PDp_10597SS1G3PD_10597SS1source2,4602,4802,5002,5202,5402,5602,5802,6002,620
    CAATACGATTACGTTGCGACGGAGCTATTAGGACATGGTAATCTACACACCGAGCCCGAGCGGCCCGCGCTTACAGCGGACGAGAGGCCATAAAAGCATCGCTGGGTTTCGAAAGTTAGACCGAAGTCTCATCTCTGATCTTCATTTCCTCTTCATCTATTTCGACCTATACTAGGTTATGCTAATGCAACGCTGCCTCGATAATCCTGTACCATTAGATGTGTGGCTCGGGCTCGCCGGGCGCGAATGTCGCCTGCTCTCCGGTATTTTCGTAGCGACCCAAAGCTTTCAATCTGGCTTCAGAGTAGAGACTAGAAGTAAAGGAGAAGTAGATAAAGCTGGATATGATCBglIEarISpeIBstBIPshAIG3PD_10597SS1G3PDp_10597SS15'UTRsource2,6402,6602,6802,7002,7202,7402,7602,7802,800
    TAACCCTAGTTTCCGCAATGCCCGTGAGTAGATGACTTCCGTTCCTCTTGCGGCGGATTGACCGTACTGGTACTTCTAGGTCAAAGTCGGAATTAACGGATGGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACATTGGGATCAAAGGCGTTACGGGCACTCATCTACTGAAGGCAAGGAGAACGCCGCCTAACTGGCATGACCATGAAGATCCAGTTTCAGCCTTAATTGCCTACCAGAAGTGTGAGCTTCTAAAGCAACCCCTGACCGCTGTCTGTCGGCCGATGTTGGACCTGGTTCAGGAACTTGBarINaeIPflFINgoMIVTth111IMV2FT4LE6DF8VG10DW12RQ14TA16GY18NL20DQ22VL24E2900-3415G3PD_10597SS1NanoLuc_GL_IDToptimizedIntron_1Exon_25'UTRsource2,8202,8402,8602,8802,9002,9202,9402,960
    AGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTCCCTCCACACAGGTCAAACAAAGTCTTAGAGCCCCACAGGCATTGAGGCTAGGTTTCCTAACAGGACTCGCCACTTTTACCCGACTTCTAGCTGTAGGTACAGTAGTAGGGCATACTTCCAGACTCGCCGCTGGTTTACCCGGTCTAGCTTTTTTAAAAATTCCACCACATGGGApoIBsaBIQ26GG28VS30SL32FQ34NL36GV38SV40TP42IQ44RI46VL48SG50EN52GL54KI56DI58HV60II62PY64EG66LS68GD70QM72GQ74IE76KI78FK80VV82YP842900-3415NanoLuc_GL_IDToptimizedsource2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGACACCTACTAGTAGTGAAATTCCACTAGGACGTGATACCGTGTGACCATTAGCTGCCCCAATGCGGCTTGTACTAGCTGATAAAGCCTGCCGGCATACTTCCGTAGCGGCACAAGCTGCCGTTTTTCTAGTGACATTGTCCCTGGGACACCTTGCCGTTGTTTTAATAGCTGCTCEagIKflIBsaBIVD86DH88HF90KV92IL94HY96GT98LV100ID102GV104TP106NM108ID110YF112GR114PY116EG118IA120VF122DG124KK126IT128VT130GT132LW134NG136NK138II140DE1422900-3415NanoLuc_GL_IDToptimizedsource3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGTAAGTAGAGGCAGATTGCAAGGTCTTGCTTTGTGCCCAGTGGCCTGAAGGTATCTATGAACTGTAAATAATTCTATCACATAGACCTCGCGGACTAGTTGGGGCTGCCGAGGGACGACAAGGCTCATTGGTAGTTGCCTCACTGGCCGACCGCCGACACGCTTGCGTAAGACCGCATTCATCTCCGTCTAACGTTCCAGAACGAAACACGGGTCACCGGACTTCCATAGATACTTGACATTTATTAAGATAGTGTATCTGGAGXmnIHaeIIRL144IN146PD148GS150LL152FR154VT156IN158GV160TG162WR164LC166ER168IL170A*1722900-3415NanoLuc_GL_IDToptimizedG3PD_10597SS13'UTRsource3,3403,3603,3803,4003,4203,4403,4603,4803,500
    AATAGATTTCATCATCTGAACTCCAGCCTCTATGGATATGCGGGTAAGTACTCACTGAGCAACAGTCCAATAACTCGACGTACTACGCAGACATGGCTGATCACGCAAACCGGAGGTACATACTTAAATAAAACGTTAAAGGCATGAGTCGGACGAACAGTCCGGCCTTTGGGTTTTATCTAAAGTAGTAGACTTGAGGTCGGAGATACCTATACGCCCATTCATGAGTGACTCGTTGTCAGGTTATTGAGCTGCATGATGCGTCTGTACCGACTAGTGCGTTTGGCCTCCATGTATGAATTTATTTTGCAATTTCCGTACTCAGCCTGCTTGTCAGGCCGGAAACCCAAAhdIScaIG3PD_10597SS1Terminator_polyA_putativesource3,5203,5403,5603,5803,6003,6203,6403,660
    ACGCTCACAACAGCGATACCCCCAATCTTGAAAGCTCCTACTACAGGGATCGATCACATTGATGATCGCCGCTTCAACGAGGCCTTCCACAGGAAGCACAGTTTGCTTCGGGCCATATCAACGCATGAATACGGATTCAAATTAGTGTACAATTAAAGTAATTTAAAGGGTATCGTGCGAGTGTTGTCGCTATGGGGGTTAGAACTTTCGAGGATGATGTCCCTAGCTAGTGTAACTACTAGCGGCGAAGTTGCTCCGGAAGGTGTCCTTCGTGTCAAACGAAGCCCGGTATAGTTGCGTACTTATGCCTAAGTTTAATCACATGTTAATTTCATTAAATTTCCCATAGCStuIBsrGIBstAPIG3PD_10597SS1Terminator_polyA_putativesource3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TTCATATATCCTGGAAATCTTCATTGGTCATGCCGGATTGCTCTGGAGGAGGGGAACACTATGCGGCTGAGAACGCCATTGTACTTCACTAATCTCCGATGGCCAACGATTTCGAGCTTGGAGCAGTTCTGGGTCCCGGATGTTGAGCACAAGGCGGGTGATTAGTGAGCTCGCGAAGTATATAGGACCTTTAGAAGTAACCAGTACGGCCTAACGAGACCTCCTCCCCTTGTGATACGCCGACTCTTGCGGTAACATGAAGTGATTAGAGGCTACCGGTTGCTAAAGCTCGAACCTCGTCAAGACCCAGGGCCTACAACTCGTGTTCCGCCCACTAATCACTCGAGCGCKflIMscINruISacIBseRIEco53kIG3PD_10597SS1Terminator_polyA_putativesource3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ATTgcgatgttgaagaccacgctgaggtgtcaatcgtcggagccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgcatggtcatagctgtttcctgagagcttggcaggtgatgacacacattaacaaatttcgtgaggagtctccTAAcgctacaacttctggtgcgactccacagttagcagcctcggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgtaccagtatcgacaaaggactctcgaaccgtccactactgtgtgtaattgtttaaagcactcctcagaggApoIBlpIStyIBbvCIPaqCIT7 terminatorFragment.REVVector.REVspacerM13 revSLiCE-forT7 ReverseAarINew...rtsource4,0404,0604,0804,1004,1204,1404,1604,1804,200
    agaagaatgccattaatttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtctcttcttacggtaattaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacagAseIBseRIBssSIBssSαIspacerorisource4,2204,2404,2604,2804,3004,3204,3404,360
    cgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccgggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccApaLIHaeIIorisource4,3804,4004,4204,4404,4604,4804,500
    • Plasmid
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    Editing disabled on read-only sequence.
    4513 bp325035003750400042502505007501000AmpRBB seq REVpOpen ori anneal REVBioBrick suffixT7 Reversesourceori2900-3415popen_midRev (DNA)F_g3pdp_popen (DNA)F_G3PDp_105971SS1 (DNA)f_nanoluc_g3pdp (DNA)R_G3PDt_pCbxOH (DNA)R_g3pdt_popen (DNA)R_G3PDt_10597SS1 (DNA)

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    4513