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LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggW286XZ066220406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228174-1031: Region 2HK284IL282SA280GI278EA276I174-1031: Region 1AmpXZ0662180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170174-1031: Region 2AmpXZ0662360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L174-1031: Region 2AmpXZ0662540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaScaIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K174-1031: Region 2AmpXZ0662720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M174-1031: Region 2AmpXZ06628809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatttttataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaCspCIXZ06621,0601,0801,1001,1201,1401,1601,1801,2001,220
    taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccattggttatccggctttagccgttttagggaatatttagttttcttatctggctctatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggPsiIXZ06621,2401,2601,2801,3001,3201,3401,3601,3801,400
    taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattXZ06621,4201,4401,4601,4801,5001,5201,5401,560
    ccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaagtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagXZ06621,5801,6001,6201,6401,6601,6801,7001,7201,740
    acgacgttgtaaaacgacggccagtgagcgcgcctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctagattaaccctagaaagataatcatatttgctgcaacattttgctgccggtcactcgcgcggagcaagtaagtgcaaaaacttgggcacctcctgcccgtctgagcgccacgtttacacaaaatgtcgcactacctcgtctacttctacgagctgtgcgacgtcttgtgcgtcgatctaattgggatctttctattagtataa5' PB ITR Longjt1009overlapXZ06621,7601,7801,8001,8201,8401,8601,8801,9001,920
    gtgacgtacgttaaagataatcatgtgtaaaattgacgcatgtgttttatcggtctgtatatcgaggtttatttattaatttgaatagatattaagttttattatatttacacttacatactaataataaattcaacaaacaatttatttatgtttatttatttattaaaaaaaacactgcatgcaatttctattagtacacattttaactgcgtacacaaaatagccagacatatagctccaaataaataattaaacttatctataattcaaaataatataaatgtgaatgtatgattattatttaagttgtttgttaaataaatacaaataaataaataattttttttBsiWI5' PB ITR Long5' PB ITR shortXZ06621,9401,9601,9802,0002,0202,0402,0602,0802,100
    caaaaactcaaaatttcttctataaagtaacaaaacttttatgagggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgagccgcccggggctccgctccggtccggcgctccccccgcatccccgagccggcagcgtgcggggtttttgagttttaaagaagatatttcattgttttgaaaatactccctgtcgggggggggtttcgggggtccctacattaatgcagggagggggcgatcccccgtcgtcgctcggcgggccccgaggcgaggccaggccgcgaggggggcgtaggggctcggccgtcgcacgcccSmaIXmaIRsrIITspMIcore insul5' PB ITR LongXZ06622,1202,1402,1602,1802,2002,2202,2402,260
    gacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcagacacctggggggatacggggaaaaggcctccacggccactagtTTCGCACGATTGCACCTTGGGAGCCTTAACCGCTTTTGAATGTCCGTCTTAGTTAATctgtcgggcccgtgccccttccaccgtgccctagcgaaaggagacttgcgaagagcgacgagaaactcggacgtctgtggacccccctatgccccttttccggaggtgccggtgatcaAAGCGTGCTAACGTGGAACCCTCGGAATTGGCGAAAACTTACAGGCAGAATCAATTASfiISmaISpeISrfIXmaITspMIcore insulattD Cp36Forward Targettarget ...primerXZ06622,2802,3002,3202,3402,3602,3802,4002,4202,440
    CCCTAATTGAACGCTCCAAAAGGTGACTTCCAATAGGGATTTATTCCTTTTAAAATTAACGGCATAATCGTGCCGCCGAATGCAACCTCGATCGAGCCGGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGGGATTAACTTGCGAGGTTTTCCACTGAAGGTTATCCCTAAATAAGGAAAATTTTAATTGCCGTATTAGCACGGCGGCTTACGTTGGAGCTAGCTCGGCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAMfeIEF-1α promoterattD Cp36Primer 1Reverse TargetTarget ...Primerprime...F-1aXZ06622,4602,4802,5002,5202,5402,5602,5802,6002,620
    GAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCTTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGEF-1α promoterXZ06622,6402,6602,6802,7002,7202,7402,7602,7802,800
    CCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGAflIISacIIEF-1α promoterXZ06622,8202,8402,8602,8802,9002,9202,9402,960
    GCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGBglIIEF-1α promoterXZ06622,9803,0003,0203,0403,0603,0803,1003,1203,140
    GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTApaIFseISacIIPspOMIEF-1α promoterXZ06623,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGBbvCIBpu10IEF-1α promoterXZ06623,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAEF-1α promoterfrag1XZ06623,5203,5403,5603,5803,6003,6203,6403,660
    CAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAATCTAGAAGCCACCatgaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatATGAGCGGAGAGAGCATGAACTTCAGTGGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTTAGATCTTCGGTGGtacttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctaTACTCGCCTCTCTCGTACTTGAAGTCACClaIXbaIBspDIBpu10IMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28SG30ES32MN34FS363742-62553 Signal/FlagEF-1α promoterfrag1RF from EF-1aCCR6homologXZ06623,6803,7003,7203,7403,7603,7803,8003,8203,840
    ATGTGTTCGATTCCAGTGAGGACTACTTCGTGTCCGTCAATACATCCTACTACAGCGTAGATTCTGAGATGCTGCTTTGCTCCCTGCAGGAGGTGCGCCAGTTCTCCCGACTCTTCGTTCCCATCGCGTATTCACTCATCTGCGTCTTTGGCCTCCTGGGGAATATCTTGGTAGTTACACAAGCTAAGGTCACTCCTGATGAAGCACAGGCAGTTATGTAGGATGATGTCGCATCTAAGACTCTACGACGAAACGAGGGACGTCCTCCACGCGGTCAAGAGGGCTGAGAAGCAAGGGTAGCGCATAAGTGAGTAGACGCAGAAACCGGAGGACCCCTTATAGAACCATCASbfIDV38FD40SS42ED44YF46VS48VN50TS52YY54SV56DS58EM60LL62CS64LQ66EV68RQ70FS72RL74FV76PI78AY80SL82IC84VF86GL88LG90NI92LV94V3742-62553 CCR6XZ0663,8603,8803,9003,9203,9403,9603,9804,0004,020
    CATCACATTTGCTTTTTATAAAAAGGCCAGATCCATGACAGACGTCTACCTGCTTAATATGGCCATTGCAGACATCCTGTTCGTCCTGACGCTCCCATTCTGGGCTGTGAGTCACGCTACCGGGGCCTGGGTGTTCAGTAATGCAACCTGCAAGCTGTTGAAGGGAATTTATGCCGTAGTGTAAACGAAAAATATTTTTCCGGTCTAGGTACTGTCTGCAGATGGACGAATTATACCGGTAACGTCTGTAGGACAAGCAGGACTGCGAGGGTAAGACCCGACACTCAGTGCGATGGCCCCGGACCCACAAGTCATTACGTTGGACGTTCGACAACTTCCCTTAAATACGGAccIPsiII96TF98AF100YK102KA104RS106MT108DV110YL112LN114MA116IA118DI120LF122VL124TL126PF128WA130VS132HA134TG136AW138VF140SN142AT144CK146LL148KG150IY152A3742-62553 CCR6XZ0664,0404,0604,0804,1004,1204,1404,1604,1804,200
    ATCAACTTTAATTGCGGTATGCTGCTGCTGACTTGCATAAGTATGGACAGATACATCGCCATAGTACAGGCAACAAAATCCTTCAGACTGAGGTCTCGAACGCTCCCTAGGTCTAAAATCATTTGTCTTGTTGTATGGGGCTTGAGCGTGATTATTTCCAGCAGTACTTTCGTGTTAGTTGAAATTAACGCCATACGACGACGACTGAACGTATTCATACCTGTCTATGTAGCGGTATCATGTCCGTTGTTTTAGGAAGTCTGACTCCAGAGCTTGCGAGGGATCCAGATTTTAGTAAACAGAACAACATACCCCGAACTCGCACTAATAAAGGTCGTCATGAAAGCACAScaIAvrIII154NF156NC158GM160LL162LT164CI166SM168DR170YI172AI174VQ176AT178KS180FR182LR184SR186TL188PR190SK192II194CL196VV198WG200LS202VI204IS206SS208TF210V3742-62553 CCR6XZ0664,2204,2404,2604,2804,3004,3204,3404,360
    TTAACCAGAAGTACAACACGCAGGGCAGCGATGTGTGTGAGCCCAAGTACCAGACGGTATCTGAGCCTATCAGGTGGAAGCTGTTGATGCTGGGTTTGGAATTGCTGTTCGGCTTTTTTATCCCACTCATGTTTATGATTTTTTGTTATACCTTCATCGTGAAGACCCTGGTCCAAATTGGTCTTCATGTTGTGCGTCCCGTCGCTACACACACTCGGGTTCATGGTCTGCCATAGACTCGGATAGTCCACCTTCGACAACTACGACCCAAACCTTAACGACAAGCCGAAAAAATAGGGTGAGTACAAATACTAAAAAACAATATGGAAGTAGCACTTCTGGGACCAGGTF212NQ214KY216NT218QG220SD222VC224EP226KY228QT230VS232EP234IR236WK238LL240ML242GL244EL246LF248GF250FI252PL254MF256MI258FC260YT262FI264VK266TL268VQ2703742-62553 CCR6XZ0664,3804,4004,4204,4404,4604,4804,5004,5204,540
    GGCACAAAACTCTAAGCGACACAAGGCCATCCGGGTAATCATCGCTGTTGTGCTGGTATTTCTGGCTTGCCAGATCCCTCATAATATGGTCCTTCTGGTAACGGCAGCCAACCTGGGCAAAATGAATCGCTCATGTCAGTCAGAAAAGCTTATCGGGTACACCAAAACGGTTACGCCGTGTTTTGAGATTCGCTGTGTTCCGGTAGGCCCATTAGTAGCGACAACACGACCATAAAGACCGAACGGTCTAGGGAGTATTATACCAGGAAGACCATTGCCGTCGGTTGGACCCGTTTTACTTAGCGAGTACAGTCAGTCTTTTCGAATAGCCCATGTGGTTTTGCCAATGCHindIIIAQ272NS274KR276HK278AI280RV282II284AV286VL288VF290LA292CQ294IP296HN298MV300LL302VT304AA306NL308GK310MN312RS314CQ316SE318KL320IG322YT324KT326VT3283742-62553 CCR6XZ0664,5604,5804,6004,6204,6404,6604,6804,7004,720
    GAGGTTCTGGCGTTCCTGCATTGTTGCCTTAACCCTGTGCTGTACGCATTTATAGGTCAGAAGTTTCGAAACTACTTCCTGAAGATTCTCAAAGACCTCTGGTGTGTAAGAAGAAAGTATAAAAGTAGTGGATTTTCATGCGCAGGACGCTACTCCGAAAACATTTCCAGACAGACTCCAAGACCGCAAGGACGTAACAACGGAATTGGGACACGACATGCGTAAATATCCAGTCTTCAAAGCTTTGATGAAGGACTTCTAAGAGTTTCTGGAGACCACACATTCTTCTTTCATATTTTCATCACCTAAAAGTACGCGTCCTGCGATGAGGCTTTTGTAAAGGTCTGTCTBstBIEV330LA332FL334HC336CL338NP340VL342YA344FI346GQ348KF350RN352YF354LK356IL358KD360LW362CV364RR366KY368KS370SG372FS374CA376GR378YS380EN382IS384RQ3863742-62553 CCR6XZ0664,7404,7604,7804,8004,8204,8404,8604,8804,900
    CCAGTGAGACGGCCGATAACGATAACGCCTCCTCCTTTACCATGaccggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCGGTCACTCTGCCGGCTATTGCTATTGCGGAGGAGGAAATGGTACtggccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGEagIKflIKpnIXcmIBsmBIEsp3IPpuMIAcc65ITS388ET390AD392ND394NA396SS398FT400MT402GG404RT406PP408SL410GP412QD414ES416CT418TA420SS422SL424AK426DT428SS430GT432GA434LH436LD438QT440PS442RQ444P3742-62553 V2 tailCCR64hADAR2 cat domainhomologXZ0664,9204,9404,9604,9805,0005,0205,0405,060
    TATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCBstEIII446PS448EG450LQ452LH454LP456QV458LA460DA462VS464RL466VL468GK470FG472DL474TD476NF478SS480PH482AR484RK486VL488AG490VV492MT494TG496TD498VK500DA502K3742-62554hADAR2 cat domainXZ0665,0805,1005,1205,1405,1605,1805,2005,2205,240
    GTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGABclIV504IS506VS508TG510TK512CI514NG516EY518MS520DR522GL524AL526ND528CH530AE532II534SR536RS538LL540RF542LY544TQ546LE548LY550LN552NK554DD556QK558RS560I3742-62554hADAR2 cat domainXZ0665,2605,2805,3005,3205,3405,3605,3805,4005,420
    TTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTABsrGIF562QK564SE566RG568GF570RL572KE574NV576QF578HL580YI582ST584SP586CG588DA590RI592FS594PH596EP598IL600EE602PA604DR606HP608NR610KA612RG614QL616RT618KI6203742-62554hADAR2 cat domainXZ0665,4405,4605,4805,5005,5205,5405,5605,5805,600
    AGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGBlpIBamHIES622GQ624GT626IP628VR630SN632AS634IQ636AW638DG640VL642QG644ER646LL648TM650SC652SD654KI656AR658WN660VV662GI664QG666SL668LS670IF672VE674PI676YF6783742-62554hADAR2 cat domainhomologXZ0665,6205,6405,6605,6805,7005,7205,7405,760
    TCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTPpuMIHincIISS680II682LG684SL686YH688GD690HL692SR694AM696YQ698RI700SN702IE704DL706PP708LY710TL712NK714PL716LS718GI720SN722AE724AR726QP728GK730AP732NF734SV7363742-62554hADAR2 cat domainXZ0665,7805,8005,8205,8405,8605,8805,9005,9205,940
    ACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTMluINW738TV740GD742SA744IE746VI748NA750TT752GK754DE756LG758RA760SR762LC764KH766AL768YC770RW772MR774VH776GK778VP780SH782LL784RS786KI788TK790PN792VY794H3742-62554hADAR2 cat domainXZ0665,9605,9806,0006,0206,0406,0606,0806,1006,120
    TGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCE796SK798LA800AK802EY804QA806AK808AR810LF812TA814FI816KA818GL820GA822WV824EK826PT828EQ830DQ832FS834LT836P*8383742-62554hADAR2 cat domainEMCV IRES SitePCRhomologXZ0666,1406,1606,1806,2006,2206,2406,2606,2806,300
    CTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAApaIAvrIIPspOMI4EMCV IRES SiteXZ0666,3206,3406,3606,3806,4006,4206,4406,460
    CCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTPmlIPaqCIHindIII4EMCV IRES SiteXZ0666,4806,5006,5206,5406,5606,5806,6006,6206,640
    AATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCKpnIBmgBIPflMIAcc65I4EMCV IRES SiteXZ0666,6606,6806,7006,7206,7406,7606,7806,8006,820
    ATGATAATATGGCCACAACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTTACTATTATACCGGTGTTGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGABsiWIRsrIIBstEIIMT2EY4KP6TV8RL10AT12RD14DV16PR18AV20RT22LA24AA26FA28DY30PA32TR34HT36VD38PD40RH42IE44RV46TE48LQ50EL526846-82191PuroR4PR_S030EMCV IRES SiteoverlapPR_S029XZ066XZ0666,8406,8606,8806,9006,9206,9406,9606,9807,000
    CTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGGCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTFL54TR56VG58LD60IG62KV64WV66AD68DG70AA72VA74VW76TT78PE80SV82EA84GA86VF88AE90IG92PR94MA96EL98SG100SR102LA104AQ106QQ108ME1106846-82191PuroRXZ0667,0207,0407,0607,0807,1007,1207,1407,160
    GGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCTCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGEagIGL112LA114PH116RP118KE120PA122WF124LA126TV128GV130SP132DH134QG136KG138LG140SA142VV144LP146GV148EA150AE152RA154GV156PA158FL160ET162SA164PR166NL1686846-82191PuroRXZ0667,1807,2007,2207,2407,2607,2807,3007,3207,340
    CCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGTGACGTGGAAGAAAACCCCGGTCCCGGATCCatggtgtctaagggcgaagaGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGCGGTGCTTGAAGAGAGACAATTTCGTTCGTCCACTGCACCTTCTTTTGGGGCCAGGGCCTAGGtaccacagattcccgcttctNcoISapIBamHIBmgBIBspQIPaqCISexAIPF170YE172RL174GF176TV178TA180DV182EV184PE186GP188RT190WC192MT194RK196PG198AA200TN202FS204LL206KQ208AG210DV212EE214NP216GP218GS220MV222SK224GE226E6846-82191PuroRP2AmtagBFP2homologoushomologXZ0667,3607,3807,4007,4207,4407,4607,4807,5007,520
    gctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagccgactaattcctcttgtacgtgtacttcgacatgtacctcccgtggcacctgttggtagtgaagttcacgtgtaggctcccgcttccgttcgggatgctcccgtgggtctggtactcttagttccaccagctcccgccgggagaggggaagcggaagctgtaggaccgatgatcgBsrGIL228IK230EN232MH234MK236LY238ME240GT242VD244NH246HF248KC250TS252EG254EG256KP258YE260GT262QT264MR266IK268VV270EG272GP274LP276FA278FD280IL282AT284S6846-82191mtagBFP2XZ0667,5407,5607,5807,6007,6207,6407,6607,6807,700
    ttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaggacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaaaggagatgccgtcgttctggaagtagttggtgtgggtcccgtaggggctgaagaagttcgtcaggaagggactcccgaagtgtaccctctctcagtggtgtatgctcctgcccccgcacgactggcgatgggtcctgtggtcggaggtcctgccgacggagtagatgttgcagtBsu36IF286LY288GS290KT292FI294NH296TQ298GI300PD302FF304KQ306SF308PE310GF312TW314ER316VT318TY320ED322GG324VL326TA328TQ330DT332SL334QD336GC338LI340YN342V6846-82191mtagBFP2XZ0667,7207,7407,7607,7807,8007,8207,8407,860
    agatcagaggggtgaacttcacatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgaaacgctgtaccccgctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaacgccaagaccacatatctagtctccccacttgaagtgtaggttgccgggacactacgtcttcttttgtgagccgaccctccggaagtggctttgcgacatggggcgactgccgccggaccttccgtctttgctgtaccgggacttcgagcacccgccctcggtagactagcgtttgcggttctggtgtatK344IR346GV348NF350TS352NG354PV356MQ358KK360TL362GW364EA366FT368ET370LY372PA374DG376GL378EG380RN382DM384AL386KL388VG390GS392HL394IA396NA398KT400TY4026846-82191mtagBFP2XZ0667,8807,9007,9207,9407,9607,9808,0008,0208,040
    tagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggactacagactggaaagaatcaaggaggccaacaacgaaacctacgtcgagcagcacgaggtggcagtggccagatactgcgacctccctagcaaactggggcacaaacttaatTAAGAATTCatctaggttctttgggcgattcttggagttctacggaccgcagatgatacacctgatgtctgacctttcttagttcctccggttgttgctttggatgcagctcgtcgtgctccaccgtcaccggtctatgacgctggagggatcgtttgaccccgtgtttgaattaATTCTTAAGAccIPacIAlwNIEcoRIRS404KK406PA408KN410LK412MP414GV416YY418VD420YR422LE424RI426KE428AN430NE432TY434VE436QH438EV440AV442AR444YC446DL448PS450KL452GH454KL456N*4586846-82191mtagBFP2XZ0668,0608,0808,1008,1208,1408,1608,1808,2008,220
    CTCGAGCTAGAGCTcGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGAGCTCGATCTCGAgCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCBclIPspXI1bGH poly(A) signalhomologXZ0668,2408,2608,2808,3008,3208,3408,3608,3808,400
    GGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACGACGTCAGGTGGCACTTTTCgatatctataacaagaaaatatatatataataagttatcacgtaagtagaacatgaaataacaatataattatcgtatgagttaaatcttaCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTGCTGCAGTCCACCGTGAAAAGctatagatattgttcttttatatatatattattcaatagtgcattcatcttgtactttattgttatattaatagcatactcaatttagaatSphIEcoRV13' PB ITR longbGH poly(A) signalXZ0668,4208,4408,4608,4808,5008,5208,5408,560
    aaagtcacgtaaaagataatcatgcgtcattttgactcacgcggtcgttatagttcaaaatcagtgacacttaccgcattgacaagcacgcctcacgggagctccaagcggcgactgagatgtcctaaatgcacagcgacggattcgcgctatttagaaagagagagcaatattttttcagtgcattttctattagtacgcagtaaaactgagtgcgccagcaatatcaagttttagtcactgtgaatggcgtaactgttcgtgcggagtgccctcgaggttcgccgctgactctacaggatttacgtgtcgctgcctaagcgcgataaatctttctctctcgttataaa13' PB ITR longXZ0668,5808,6008,6208,6408,6608,6808,7008,7208,740
    caagaatgcatgcgtcaattttacgcagactatctttctagggttaatctagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagttcttacgtacgcagttaaaatgcgtctgatagaaagatcccaattagatcgacgtagtcctagtatagcagcccagaaaaaaggccgagtcagtagcgggttcgaccgcgatagacccgtagcccctccttcttcgggcacggaaaagggcgctccaacttcgccgtacctttNsiISphI13' PB ITR long3' PB ITR shortjt1010XZ0668,7608,7808,8008,8208,8408,8608,8808,9008,920
    gagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgactcaaacggctcctactgacgacgacgtaactgcaactcgcttttgcgtgcaaatggtactactaagcccttccacaccggtacgtgcggaaattgccacttgacaagcaagtccggtggaccctatggtcaagcagcgccgaaaaggcctgtgtcaaggcctaccagtcgggct1XZ0668,9408,9608,9809,0009,0209,0409,0609,0809,100
    agcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagttacccggcgggcgcgcttggcgtcgcgtagtcgttgggcttgttatggccgctgtcggccttgacggcacggccacacgtctaattactgtcgccacgccgcgaccctataatgcagtcgctcctgcccataggaccgacctacggcgtctttacctgtacctatggggcactcaatgggccgcccgcgcgaaccgc1XZ0669,1209,1409,1609,1809,2009,2209,2409,260
    taatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcg1XZ0669,2809,3009,3209,3409,3609,3809,4009,4209,440
    tgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaaacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttSapIBspQI1XZ0669,4609,4809,5009,5209,5409,5609,5809,6009,620
    catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggag1XZ06629,6409,6609,6809,7009,7209,7409,7609,7809,800
    gtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaXZ06629,8209,8409,8609,8809,9009,9209,9409,960
    tatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagaAlwNIXZ06629,98010,00010,02010,04010,06010,08010,10010,12010,140
    gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttXZ066210,16010,18010,20010,22010,24010,260
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    10274 bp75008000850090009500100005001000150020002500XZ0661EF-1α promoterPrimer 1primer from EF-1a3' PB ITR shortjt1010Amp2174-1031: Region 1174-1031: Region 26846-8219XZp044.6 (DNA)oLSM0270 (DNA)oLSM0658 (DNA)XZp185.3 (DNA)XZp046.1 (DNA)XZp031.2 (DNA)XZp032.3 (DNA)EKo330.4 (DNA)XZp018.4 (DNA)XZp044.4 (DNA)oLSM0085 (DNA)pUC_oriR_Rv2 (DNA)oLSM-pUC_ori_R (DNA)oLSM-pUC_ori_F (DNA)pUC_oriR_Fv2 (DNA)AhdIScaICspCIPsiIoverlapjt1009,5' PB ITR short,BsiWI5' PB ITR Longcore insul,TspMI,XmaI,SmaI,RsrIITspMI,XmaI,SmaI,SrfIForward Target,target forward primer,+2attD Cp36Reverse TargetTarget Reverse PrimermTagBFP2.1 (DNA)Bsu36ImtagBFP2AccIAlwNIhomolog,XZp044.5 (DNA),PacI,EcoRI,PspXI,+1bGH poly(A) signaloLSM0086 (DNA)SphI,EcoRV3' PB ITR longNsiISphIBspQISapIAlwNI

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    10274

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