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pTS055(dnaB)
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcgtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactcaggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagNciINciINspIAlwNIBpuEIEcoP15Ip15A ori20406080100120140160
    atgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaatacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttBpuEIBstBIp15A ori180200220240260280300320340
    gatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgcaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaactggatccttactcgagtctagactgcaggcggatcttcaccctagtagaataattagtctattttataaagatctaaagtcacgttaaatagagaagtttacgtccgtagtttattttgctttccgagtcagctttctgacccggaaagcaaaatagacaacaaacagccacttgacctaggaatgagctcagatctgacgtccgcctagaagtggAseIAvaIPstISspITliIXhoIBamHIBsoBIPspXIPaeR7IrrnB T1 terminator360380400420440460480500520
    tagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacaggacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagaatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcctgtaataaacggctgatggaaccactagagcggaaagtgcatcacctgtttaagaaggttgactagacgcgcgctccggttcgctagaagaagaacaggttctBsaAIBssHIIDraIIISmR540560580600620640660680700
    taagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccattcggacagatcgaagttcatactgcccgactatgacccggccgtccgcgaggtaacgggtcagccgtcgctgtaggaagccgcgctaaaacggccaatgacgcgacatggtttacgccctgttgcattcgtgatgtaaagcgagtagcggtcgggtcagcccgccgctcaaggBglIFauINaeIBsmFIBsrDIBtgZINgoMIVSmR720740760780800820840860
    atagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcatttatcgcaattccaaagtaaatcgcggagtttatctaggacaagtccttggcctagtttctcaaggaggcggcgacctggatggttccgttgcgatacaagagaacgaaaacagtcgttctatcggtctagttacagctagcaccgaccgagcttctatggacgttcttacagtaaPvuIAvaIIBfuAIBspMIBsrDISmR8809009209409609801,0001,0201,040
    gcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttcgcgacggtaagaggtttaacgtcaagcgcgaatcgacctattgcggtgccttactacagcagcacgtgttgttaccactgaagatgtcgcgcctcttagagcgagagaggtccccttcggcttcaaaggttttccagcaactagtttcgagcggcgcaacaaagtagttcggaaAjuIBclIBlpIBpmIBtgIMslITfiIApaLIBaeGIBsiHKAIBsp1286ISmR1,0601,0801,1001,1201,1401,1601,1801,2001,220
    acggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatactcttcctgccagtggcattggtcgtttagttatagtgacacaccgaagtccggcggtaggtgacgcctcggcatgtttacatgccggtcgttgcagccaagctctaccgcgagctactgcggttgatggagactatcaactcagctatgaagccgctagtggcgaagggagtatgagaaggAcuIBtsIEaeIHgaIBsaHIBtsαIBstEIIDraIIISmR1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagctagctcactcggtcgctaccagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatcgatcgagtgagccagcgatggtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggBmtINheISspIBciVIBciVIBspHIBspHIAmpR promoterAmpR promoter1,4201,4401,4601,4801,5001,5201,5401,560
    gcgcactaggcgtatcacgaggcagaatttcTCCTCTGGCGGAAAGCCTctaacatcagtgcctatgcggacatcgtgcgtgaacgtGTGgttGTAcgtgagatgatctcggttgcgaatgagattgccgaagcgatcTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTtccgcgtgatccgcatagtgctccgtcttaaagAGGAGACCGCCTTTCGGAgattgtagtcacggatacgcctgtagcacgcacttgcaCACcaaCATgcactctactagagccaacgcttactctaacggcttcgctagAACTGTCGATCGAGTCAGGATCCATATTATGATCAagBmtINheISpeIAvrIIBsaAIBssSIAflIIIBssSαIoligohomology sequence (here: dnaB(A130V)))PJ23119sub-li...pacer1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gcaaccgagatcatctcaGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTGAAGCTTGGGCCCGAACAAAAACTCatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatccgttggctctagtagagtCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGCTCAGCCACGAAAAAAACTTCGAACCCGGGCTTGTTTTTGAGtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagAccIApaIBanISalIBaeGIBanIIHincIIPspOMIHindIIIBsp1286IsgRNAoligodCas9 handleS. pyogenes terminatoroverlapprotospacer1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagctaactcaaatttgccagaggtcgaaccgacaaaaccgcctactctcttctaaaagtcggactatgtctaatttagtcttgcgtcttcgccagactattttgtcttaaacggaccgccgtcatcgcgccaccagggtggactggggtacggcttgagtcttcactttgcggcatcgBpmIBsaIPmeIXmnIAvaIIBsmFIrrnB_terminator1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACTGGATCCTTACTCGAGTCTAGACTGCAGgtcacactggctcaccttcgggtgggcctcggctaccatcacaccccagaggggtacgctctcatcccttgacggtccgtagtttattttgctttccgagtcagctttctgaCCCGGAAAGCAAAATAGACAACAAACAGCCACTTGACCTAGGAATGAGCTCAGATCTGACGTCcagtgtgaccgagtggaagcccacccggaAhdIAvaIBsaIPstITliIXhoIBamHIBfuAIBsoBIBspMIPspXIPaeR7IrrnB_terminatorT7Te terminator2,1202,1402,1602,1802,2002,2202,2402,260
    ttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataagacgcaaatatggatccctatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgcctttaccgaatgcttgccccgcctctaaaggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtaBtgIPfoIAvrIISacII2,2802,3002,3202,3402,3602,3802,4002,4202,440
    aggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctctccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagAgeIEaeIHgaIBssSIBssSαIp15A ori2,4602,4802,5002,5202,5402,5602,5802,6002,620
    attccacgcctgacacttaaggtgcggactgtgap15A ori2,640
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    2642 bp1750200022502500250500SmRpTS055_R (DNA)library_spec_F (DNA)Ec-F-control (Larson et al. 2013) (DNA)CREATE_insertR (general) (Garst et al. 2017) (DNA)seq_R (DNA)

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