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pAX1-Em (Archived)
  • Sequence Map
    Editing disabled on archived sequence.
    GGGCGAATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGCGGCCGCGGGAATTCGATcacgcccgttattaatcgaagaagaggcggctaagctggtcaacgaagacgaaatccgtgctgcagcgatcgaagcttgcgaacagaatgggatcgtctttattgatgagatcgaCCCGCTTAACCCGGGCTGCAGCGTACGAGGGCCGGCGGTACCGCCGGCGCCCTTAAGCTAgtgcgggcaataattagcttcttctccgccgattcgaccagttgcttctgctttaggcacgacgtcgctagcttcgaacgcttgtcttaccctagcagaaataactactctagctApaIBbsIBlpIBtgIBtgIClaINcoINotISphIStyIZraIAatIIBspDISacIIPspOMIHindIIIPD_070220406080100120140160
    taaggtcgttaaacgtggtgatactgttggcggcggtgatgtgagtcgcgaaggtgtgcagcgcgatttgctgccattggtagaggggtcgaatgtgtctaccaagtacggcacgatcaggaccaaccacattttgttcattgcttccggtgcctttcacctcaccaagccaagcattccagcaatttgcaccactatgacaaccgccgccactacactcagcgcttccacacgtcgcgctaaacgacggtaaccatctccccagcttacacagatggttcatgccgtgctagtcctggttggtgtaaaacaagtaacgaaggccacggaaagtggagtggttcggttcgAccIBsgINruIPD_0702180200220240260280300320340
    gatttaattccggagttgcaaggccgtttcccgattcgggtggagttggatgcgctgagtaaagcggattttatccgtatcctgacagagccgaaggcggcgctcactaagcagtatcaggaattgttgaaaacggaaggtgtgtcgcttgattttacagaggatgcgattgaccctaaattaaggcctcaacgttccggcaaagggctaagcccacctcaacctacgcgactcatttcgcctaaaataggcataggactgtctcggcttccgccgcgagtgattcgtcatagtccttaacaacttttgccttccacacagcgaactaaaatgtctcctacgctaactggBspEIPD_0702360380400420440460480500520
    gtattgccgagattgcttatttggtcaacgagcgtcaggagaatatcggtgcgcggcgtttgcacacggtgttggagcgattgttggaaacgctgagttatgagtctccggatcgtgatggcgagtccgtgacggtcgatgccgattatgtcaatgcgcatttgggggaattagtcataacggctctaacgaataaaccagttgctcgcagtcctcttatagccacgcgccgcaaacgtgtgccacaacctcgctaacaacctttgcgactcaatactcagaggcctagcactaccgctcaggcactgccagctacggctaatacagttacgcgtaaacccccttaatcaBspEIFspAIDraIIINmeAIIIPD_0702540560580600620640660680700
    gaaggatccagatctgagtcgttacattttgtaatgtgtaaggcatcgtgcgatcttcattgtttgccagcgaggcgtatcgttattctacggtgttctctatctggtagaggacgatgctgttaaccattgtTCTAGCTAGTAAGCTTGGATCCTCGAGATCTAGACAATTGAGcttcctaggtctagactcagcaatgtaaaacattacacattccgtagcacgctagaagtaacaaacggtcgctccgcatagcaataagatgccacaagagatagaccatctcctgctacgacaattggtaacaAGATCGATCATTCGAACCTAGGAGCTCTAGATCTGTTAACTCAvaIMfeITliIXbaIXhoIBamHIBamHIBsaBIBsoBIPaeR7IHindIIIPD_0702720740760780800820840860
    ATAAAAAAAATCCTTAgctttcgctaaggatgatttctagcgatgaccctgctgattggttcgctgaccatttccgggtgcgggacggcgttaccagaaactcagaaggttcgtccaaccaaaccgactctgacggcagtttacgagagagatgatagggtctgcttcagtaagcTATTTTTTTTAGGAATcgaaagcgattcctactaaagatcgctactgggacgactaaccaagcgactggtaaaggcccacgccctgccgcaatggtctttgagtcttccaagcaggttggtttggctgagactgccgtcaaatgctctctctactatcccagacgaagtcattcgBsmFIL320FF318G*316SE314SL312II310EL308SS306GA304SQ302NA300SW298KR296TR294SP292TV290LF288ES286PE284DL282WV280SE278SP276LK274RS272LH270YP268RS266*Y264A875-18368809009209409609801,0001,0201,040
    cagatgctacacaattaggcttgtacgggtactcgacctgcatcccttaacttacttattaaataatttatagctattgaaaagagataagaattgttcaaagctaatattgtttaaatcgtcaattcctgcatgttttaaggaattgttaaattgattttttgtaaatattttcgtctacgatgtgttaatccgaacatgcccatgagctggacgtagggaattgaatgaataatttattaaatatcgataacttttctctattcttaacaagtttcgattataacaaatttagcagttaaggacgtacaaaattccttaacaatttaactaaaaaacatttataaaagBfuAIBspMIL262H*260VI258LS256TR254TS252SR250CG248KV246*K244NF242LK240YS238NF236LS234LF232QE230FS228IN226NL224DD222IG220AH218KL216SN214NF212QN210KT208FI206K875-1836ermC1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttgtattctttgttaacccatttcataacgaaataattatacttttgtttatctttgtgtgatattcttgatttttttctacttaatctgataagtgagctattcactttaggtttaggatgaaaatattctcttggaaccatacttaatatagaaatatcaacttctgccattaaacataagaaacaattgggtaaagtattgctttattaatatgaaaacaaatagaaacacactataagaactaaaaaaagatgaattagactattcactcgataagtgaaatccaaatcctacttttataagagaaccttggtatgaattatatctttatagttgaagacggtaatXmnIK204YE202KN200VW198KM196VF194YN192YK190QK188DK186HS184IR182SK180KR178SL176RI174LS172SN170VK168PK166PH164FY162ER160PV158MS156LI154SI152DV150EA148ML146875-1836ermC1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaagtaatgccaatgagcgttttgtatttaataatcttttagcaaacccgtattccacgattaaataaatctcattagctatactatcaaaaacaattttgcgtattatatccgtacttatgttataaggtatattaccatatattttataggattggtttttaggaaatttaaatttcattacggttactcgcaaaacataaattattagaaaatcgtttgggcataaggtgctaatttatttagagtaatcgatatgatagtttttgttaaaacgcataatataggcatgaatacaatattccatataatggtatataaaatatcctaaccaaaaatcctttaaatttLL144AL142SR140KT138NL136LR134KA132FG130YE128VI126LY124IE122NA120IS118DF116VI114KR112II110DT108SI106NY104PI102NG100YI98KY96SQ94NK92PF90KF88875-1836ermC1,4201,4401,4601,4801,5001,5201,5401,560
    ctgcaatatatccttgtttaaaacttggaaattatcgtgatcaacaagtttattttctgtagttttgcataatttatggtctatttcaatggcagttacgaaattacacctctttactaattcaagggtaaaatggccttttcctgagccgatttcaaagatattatcatgttcagacgttatataggaacaaattttgaacctttaatagcactagttgttcaaataaaagacatcaaaacgtattaaataccagataaagttaccgtcaatgctttaatgtggagaaatgattaagttcccattttaccggaaaaggactcggctaaagtttctataatagtacaagtBclIQL86ID84KN82LV80QF78ND76HD74VL72KN70ET68TK66CL64KH62DI60EI58AT56VF54NC52RK50VL48EL46TF44HG42KG40SG38IE36FI34ND32HE30875-1836ermC1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tttaatcttatatttgtcattattttatctatattatgttttgaagtaataaagttttgactgtgttttatatttttctcgttcattataaccctctttaatttggttatatgaattttgcttattaacgattcattataaccacttattttttgtttggttgataatgaactgtaaattagaatataaacagtaataaaatagatataatacaaaacttcattatttcaaaactgacacaaaatataaaaagagcaagtaatattgggagaaattaaaccaatatacttaaaacgaataattgctaagtaatattggtgaataaaaaacaaaccaactattacttgacaNL28RI26NT24MI22KD20IN18HK16ST14IF12NQ10SH8KI6NK4EN2M875-1836ermC1,7601,7801,8001,8201,8401,8601,8801,9001,920
    gctgattacaaaaatactaaaaatgcccatattttttcctccttataaaattagtataattatagcacgcgaattcatcgaatttctgccattcatccgcttattaTCACTTATTCAGGCGTAGCAAAtagaggacgatgctgttaaccattgtcatcaatggcactatccatgtcgactaatgtttttatgatttttacgggtataaaaaaggaggaatattttaatcatattaatatcgtgcgcttaagtagcttaaagacggtaagtaggcgaataatAGTGAATAAGTCCGCATCGTTTatctcctgctacgacaattggtaacagtagttaccgtgataggtacaBsrGIPD_07031,9401,9601,9802,0002,0202,0402,0602,0802,100
    acatgcaagcgttattcaaagcccgagcttgtcgtagtcgtcgcatcgggttggtggtggggggcatcagtacagtggtgtaccagcgtggtaggccgtgacgaagccccgccaatccagcatgacttgtgtagcgtagttaaatgcgaaccgtcgcagttctttctctaactgttgtacgttcgcaataagtttcgggctcgaacagcatcagcagcgtagcccaaccaccaccccccgtagtcatgtcaccacatggtcgcaccatccggcactgcttcggggcggttaggtcgtactgaacacatcgcatcaatttacgcttggcagcgtcaagaaagagattgacaAvaIBsoBIPD_07032,1202,1402,1602,1802,2002,2202,2402,260
    tttttatgactgacggtgttgtggatttgcttgtcgatgttgtatccgaccacactcagatcattatcttggttggccagtgtatgtgctgaggccagcgcttgacctagatagagggcggcgatggtcatttttccggctgtattgagtgtttctgacgctagatcttcctgataaaaatactgactgccacaacacctaaacgaacagctacaacataggctggtgtgagtctagtaatagaaccaaccggtcacatacacgactccggtcgcgaactggatctatctcccgccgctaccagtaaaaaggccgacataactcacaaagactgcgatctagaaggactaAfeIMscIBbvCIBsaBIPD_07032,2802,3002,3202,3402,3602,3802,4002,4202,440
    acggtgatttttcatgcacgtagtattgtgtgtcgccgatgctggtatagccaatgagcacatcagcatgtagtagctgtgcttgagctgtgcgtgctacgcgcgctccctcgtggttgtggtagatggaggcgggcatctgtgttggtgcagcaatggcaaccacactgcgacttgccactaaaaagtacgtgcatcataacacacagcggctacgaccatatcggttactcgtgtagtcgtacatcatcgacacgaactcgacacgcacgatgcgcgcgagggagcaccaacaccatctacctccgcccgtagacacaaccacgtcgttaccgttggtgtgacgctgaBsgIBssHIIPD_07032,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttcctgtttccattctaggatgacgtcgtcgtcagtggcggtgcttggaccttcgatgagtacgtatagacggcgcttacctaggctgctggtgccagatctgagtttctgtcgtacgtctttaatcgtgtagtagtcggcagcatagcgtttggatggatgaatggaggccaggaaggacaaaggtaagatcctactgcagcagcagtcaccgccacgaacctggaagctactcatgcatatctgccgcgaatggatccgacgaccacggtctagactcaaagacagcatgcagaaattagcacatcatcagccgtcgtatcgcaaacctacctacttacctccggtccStyIZraIAatIIAvrIIBsiWIPflFISnaBITth111IPD_07032,6402,6602,6802,7002,7202,7402,7602,7802,800
    tagctgtccattgctgcagccacgccgctatgggtgtttgcatctacgctgacaaggttaATCACTAGTGAATTCGCGGCCGCCTGCAGGTCGACCATATGGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCTTGGCGTAATCATGatcgacaggtaacgacgtcggtgcggcgatacccacaaacgtagatgcgactgttccaatTAGTGATCACTTAAGCGCCGGCGGACGTCCAGCTGGTATACCCTCTCGAGGGTTGCGCAACCTACGTATCGAACTCATAAGATATCACAGTGGATTTATCGAACCGCATTAGTACAccIMluINdeINotINsiISacISalISbfISpeIBfuAIBspMIBstXIAflIIIEco53kIPD_0703Sp6_primer2,8202,8402,8602,8802,9002,9202,9402,960
    GTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTPvuIIlac_promoterM13_pUC_rev_primerM13_...mer2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCPciISapIBspQIAflIII3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAG3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCAApaLIBseYI3,5203,5403,5603,5803,6003,6203,6403,660
    AACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCTTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGAlwNI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAAT3,8603,8803,9003,9203,9403,9603,9804,0004,020
    AGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAABspHIAmpicillinORF frame 34,0404,0604,0804,1004,1204,1404,1604,1804,200
    GCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCCGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGAhdIBmrIBpmIBsaIBsrFINmeAIIIAmpicillinORF frame 34,2204,2404,2604,2804,3004,3204,3404,360
    AGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAATCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTAclIAmpicillinORF frame 34,3804,4004,4204,4404,4604,4804,5004,5204,540
    AAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCScaIAmpicillinORF frame 34,5604,5804,6004,6204,6404,6604,6804,7004,720
    CGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAAclIXmnIApaLIAmpicillinORF frame 34,7404,7604,7804,8004,8204,8404,8604,8804,900
    CTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATBspHIAmpicillinORF frame 3AmpR_promoter4,9204,9404,9604,9805,0005,0205,0405,060
    GAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAACTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCTTTAACATTCGCAATTATAAAACAATTTTAAGCGCAATTTAAAAACAATTTAGTCGAGTAAAAAATTGGTT5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTAloIDraIIIf1_origin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGNaeIBsrFINgoMIVf1_origin5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCAAAAGGGTCAGTGCTGCAABmrIPvuIIlacZ_af1_originM13_...mer5,6205,6405,6605,6805,7005,7205,7405,760
    GTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATACATTTTGCTGCCGGTCACTTAACATTATGCTGAGTGATATlacZ_aM13_f...imerT7_promoter5,7805,800
    • Plasmid
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    • Linear Map
    Editing disabled on archived sequence.
    5815 bp42504500475050005250550025050075010001250ermCM13_pUC_fwd_primerM13_forward20_primer875-1836PspOMI,ApaI,ZraI,AatII,SphI,BtgI,NcoI,StyI,NotI,BtgI,SacII,BlpI,BbsI,HindIII,+5PD_0702,BspEINmeAIII,DraIII,BspEIFspAI,BamHI,BsaBIHindIII,BamHI,AvaI,BsoBI,PaeR7I,TliI,XhoI,XbaI,MfeIBsmFIBfuAIBspMIXmnIAclIAmpicillin,ORF frame 3ScaIAclI,XmnIApaLIBspHIAmpR_promoterf1_origin,AloI,DraIIIlacZ_a,T7_promoter,BsrFI,NgoMIV,NaeI,PvuII,BmrI

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    5815