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yeast made reindeer milk
  • Sequence Map
    Editing disabled on read-only sequence.
    ggagcgacctcatgctatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcttatttagcctcgctggagtacgatatggactctttcgttggactggatgtcctttctcaatgagttcttattcttaaaagcaaaattttggattctcagtgaaattttaaacatatgtgaataaaaaaaatattgaataaattattatttttagtatttagtattctttaagcgaataaatcBstZ17IADH1 terminator20406080100120140160
    aagtgtcaacaacgtatctaccaacgatttgacccttttccatcttttcgtaaatttctggcaaggtagacaagccgacaaccttgattggagacttgaccaaacctctggcgaagaattgttaattaagagctcagatcttatcgtcgtcatccttgtaatccatcgatactagttcacagttgttgcatagatggttgctaaactgggaaaaggtagaaaagcatttaaagaccgttccatctgttcggctgttggaactaacctctgaactggtttggagaccgcttcttaacaattaattctcgagtctagaatagcagcagtaggaacattaggtagctatgatcPacISacISpeIBglIIEco53kIFLAG180200220240260280300320340
    tgcggccgcttgttttatatttgttgtaaaaagtagataattacttccttgatgatctgtaaaaaagagaaaaagaaagcatctaagaacttgaaaaactacgaattagaaaagaccaaatatgtatttcttgcattgaccaatttatgcaagtttatatatatgtaaatgtaagacgccggcgaacaaaatataaacaacatttttcatctattaatgaaggaactactagacattttttctctttttctttcgtagattcttgaactttttgatgcttaatcttttctggtttatacataaagaacgtaactggttaaatacgttcaaatatatatacatttacattcEagINotI360380400420440460480500520
    tttcacgaggttctactaaactaaaccacccccttggttagaagaaaagagtgtgtgagaacaggctgttgttgtcacacgattcggacaattctgtttgaaagagagagagtaacagtacgatcgaacgaactttgctctggagatcacagtgggcatcatagcatgtggtactaaagtgctccaagatgatttgatttggtgggggaaccaatcttcttttctcacacactcttgtccgacaacaacagtgtgctaagcctgttaagacaaactttctctctctcattgtcatgctagcttgcttgaaacgagacctctagtgtcacccgtagtatcgtacaccatga540560580600620640660680700
    aaaccctttcccgccattccagaaccttcgattgcttgttacaaaacctgtgagccgtcgctaggaccttgttgtgtgacgaaattggaagctgcaatcaataggaagacaggaagtcgagcgtgtctgggttttttcagttttgttctttttgcaaacaaatcacgagcgacggtttgggaaagggcggtaaggtcttggaagctaacgaacaatgttttggacactcggcagcgatcctggaacaacacactgctttaaccttcgacgttagttatccttctgtccttcagctcgcacagacccaaaaaagtcaaaacaagaaaaacgtttgtttagtgctcgctgccAhdI720740760780800820840860
    taatttctttctcgataagaggccacgtgctttatgagggtaacatcaattcaagaaggagggaaacacttcctttttctggccctgataatagtatgagggtgaagccaaaataaaggattcgcgcccaaatcggcatctttaaatgcaggtatgcgatagttcctcactctttattaaagaaagagctattctccggtgcacgaaatactcccattgtagttaagttcttcctccctttgtgaaggaaaaagaccgggactattatcatactcccacttcggttttatttcctaagcgcgggtttagccgtagaaatttacgtccatacgctatcaaggagtgagaaaPmlIBsaAI8809009209409609801,0001,0201,040
    ccttactcacgagtaattcttgcaaatgcctattatgcagatgttataatatctgtgcgtcttgagttgaagtcaggaatctaaaataaaaattaaggttaataaaaagaggaaagaaaaaaaaattaatcgatttacagaaacttgcacactaaaaatacacaactaaaagcaaggaatgagtgctcattaagaacgtttacggataatacgtctacaatattatagacacgcagaactcaacttcagtccttagattttatttttaattccaattatttttctcctttctttttttttaattagctaaatgtctttgaacgtgtgatttttatgtgttgattttcgtt1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttacagtatgggaagtcatcgacgttatctctactatagtatattatcatttctattattatcctgctcagtggtacttgcaaaacaagataagaccccattctttgaaggtacttccaggccggccgcacacaccatagcttcaaaatgtttctactccttttttactcttccaaatgtcatacccttcagtagctgcaatagagatgatatcatataatagtaaagataataataggacgagtcaccatgaacgttttgttctattctggggtaagaaacttccatgaaggtccggccggcgtgtgtggtatcgaagttttacaaagatgaggaaaaaatgagaaggtEagIFseINaeINgoMIVTEF1 promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    gattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttctaaaagagcctgaggcgcgtagcggcatggtgaagttttgtgggttcgtgtcgtatgatttaaaggggagaaagaaggagatcccacagcaattaatgggcatgatttccaaaccttttcttttttctctggcggagcaaagaaaaagaagcagctttttccgttatttttaaaBplIBplIBsaITEF1 promoter1,4201,4401,4601,4801,5001,5201,5401,560
    ttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaataatagtgcaaagaaaaagaacttttaaaaaaaaaaactaaaaaaagagaaagctactggagggtaactataaattcaattatttgccagaagttaaagagttcaaagtcaaagtaaaaagaacaagataatgttgaaaaaaatgaagaacgagtaatctttctttcgtatcgttaTEF1 promoter1,5801,6001,6201,6401,6601,6801,7001,7201,740
    ctaatctaagttttaattacaaggatccgtaatacgactcactatagggcccgggcgtcgacatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtgattagattcaaaattaatgttcctaggcattatgctgagtgatatcccgggcccgcagctgtactctaaaggaagttaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaaggccgacttcgacaApaISalISmaISrfIXmaIBamHIBsaHITspMIPspOMIα-factor secretion signalα-factor secretion signalT7 promoterTE...r1,7601,7801,8001,8201,8401,8601,8801,9001,920
    catcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctATGCCTCTTAACACCATTTACgtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccaataacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgaTACGGAGAATTGTGGTAAATGα-factor secretion signalbeta caseinα-f...al1,9401,9601,9802,0002,0202,0402,0602,0802,100
    AAGCAACCACAGAACCAAATTATAATCCACTCTGGAAGCCCCAGTTTGTTGGTCCTTTATTTTGGTAAGGAAGAACTGAGAGCAATGAAGGTATTGATTTTAGCCTGTCTCGTGGCGCTAGCTTTGGCAAAGGAGGTTAACACTGAGAAAAATGTAGAAATAAATAAGACAAGGATTCGTTGGTGTCTTGGTTTAATATTAGGTGAGACCTTCGGGGTCAAACAACCAGGAAATAAAACCATTCCTTCTTGACTCTCGTTACTTCCATAACTAAAATCGGACAGAGCACCGCGATCGAAACCGTTTCCTCCAATTGTGACTCTTTTTACATCTTTATTTATTCTGTTCCTBmrIBmtIHpaINheIbeta casein2,1202,1402,1602,1802,2002,2202,2402,260
    CCGTTAGACTGTGCGTGGAAAATCGAATCACAAACATTGTTAATCAAAAAATCGAAAAACTGCAAAACGAGGGTCAACAGCAAACTGAAGATGAATTGCAAGACAAAATCCATCCCTTCGCTCAAACGCAATCATTAGTGTACCCATTTACTGGCCCGATTCCGTATTCCCTGCCGGCAATCTGACACGCACCTTTTAGCTTAGTGTTTGTAACAATTAGTTTTTTAGCTTTTTGACGTTTTGCTCCCAGTTGTCGTTTGACTTCTACTTAACGTTCTGTTTTAGGTAGGGAAGCGAGTTTGCGTTAGTAATCACATGGGTAAATGACCGGGCTAAGGCATAAGGGACGGbeta casein2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ACAAAATTTTCTTCCACTACCACAGACACCAGTGATGGTGTCACCCTTTTTGCAACCGGAGATTATGGGGGTTAGTGAAGTAAAAGAAACCATGGTCCCTAAACACAAAGAAATGCCTTTCCCCAAATATCCTGTCGAGCCATTTGCAGAAGGTCAGTCTTTAACTTTAACTGAATGTTTTAAAAGAAGGTGATGGTGTCTGTGGTCACTACCACAGTGGGAAAAACGTTGGCCTCTAATACCCCCAATCACTTCATTTTCTTTGGTACCAGGGATTTGTGTTTCTTTACGGAAAGGGGTTTATAGGACAGCTCGGTAAACGTCTTCCAGTCAGAAATTGAAATTGACTTbeta casein2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GTTGAGAATTTGCATTTGCCTTTACCATTACCCCAATCATGGATGCATCAAACACCTCAGCCACTCCCACCAACAGTTATGTTTCCGCCTCAATCCGTTCTTAGCCTATCTCAGCCCAAAGTCTTATCTGTTCCACAAAAGGCCGTCCCATACCCACAACGTGATATGCCTATACCAACTCTTAAACGTAAACGGAAATGGTAATGGGGTTAGTACCTACGTAGTTTGTGGAGTCGGTGAGGGTGGTTGTCAATACAAAGGCGGAGTTAGGCAAGAATCGGATAGAGTCGGGTTTCAGAATAGACAAGGTGTTTTCCGGCAGGGTATGGGTGTTGCACTATACGGATATGBbvCIDraIIIbeta casein2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AGGCTTTTTTATTATATCAAGAACCAGTACCAGGACCTGTTAGAGGTCCTTTCCCTATTATAGTTTCGCTAAATTTGTTGACGGTACTATTCAATTTCGGTTCTGGTGCTACTAACTTTTCATTGTTAAAACAAGCAGGCGATGTTGAGGAAAATCCAGGACCTatgagatttccTCCGAAAAAATAATATAGTTCTTGGTCATGGTCCTGGACAATCTCCAGGAAAGGGATAATATCAAAGCGATTTAAACAACTGCCATGATAAGTTAAAGCCAAGACCACGATGATTGAAAAGTAACAATTTTGTTCGTCCGCTACAACTCCTTTTAGGTCCTGGAtactctaaaggbeta caseinp2aα-...l2,8202,8402,8602,8802,9002,9202,9402,960
    ttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggaagttaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaaggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccα-factor secretion signalα-factor secretion signal2,9803,0003,0203,0403,0603,0803,1003,1203,140
    ttattgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctATGATGAAATCTTTCTCTTTAGCGGTTACCATATTGGCTCTCACGTTACCATTTTTAGTTGCTCAAGAGCAGAACCAGGAACAGCCTACTGGTTaataacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgaTACTACTTTAGAAAGAGAAATCGCCAATGGTATAACCGAGAGTGCAATGGTAAAAATCAACGAGTTCTCGTCTTGGTCCTTGTCGGATGACCAAkappa caseinα-factor secretion signalα-f...al3,1603,1803,2003,2203,2403,2603,2803,3003,320
    GCGAAAAAGATGAGAGGTTTTTCAACGATAAGATCGTAAAATATATACCAATACAATACGCACTTTCTCGATATCCAAGCTACGGCTTATCCTACTATCAACATAGACCCGTGGCGCTTATCAATAACCAATTTCTGCCTTATCCTTACTATGCCAAGCCGGGAGCAGTTAGATCCGCTTTTTCTACTCTCCAAAAAGTTGCTATTCTAGCATTTTATATATGGTTATGTTATGCGTGAAAGAGCTATAGGTTCGATGCCGAATAGGATGATAGTTGTATCTGGGCACCGCGAATAGTTATTGGTTAAAGACGGAATAGGAATGATACGGTTCGGCCCTCGTCAATCTAGBsgIkappa casein3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ACCTGCACAAATTTTGCAATGGCAAGTCCTACCTAATACTGTCCCGGCTAAGTCCTGTCAAGCACAACCAACTACATTGGCCCGTCACCCACATCCAAGATTGAGCTTTATGGCTATTCCTCCAAAGAAAAATCAAGACAAAACTGACATTCCCACAATAAATACTATCGCTACTTGGACGTGTTTAAAACGTTACCGTTCAGGATGGATTATGACAGGGCCGATTCAGGACAGTTCGTGTTGGTTGATGTAACCGGGCAGTGGGTGTAGGTTCTAACTCGAAATACCGATAAGGAGGTTTCTTTTTAGTTCTGTTTTGACTGTAAGGGTGTTATTTATGATAGCGATGAPaqCIkappa casein3,5203,5403,5603,5803,6003,6203,6403,660
    GTCGAATCAACCATTACTCCCACAACAGAAGCAATTGTAGATACGGTTGCCACCCTAGAAGCCTCGAGTGAAGTGATTGAAAGTGCTCCAGAGACAAATACCGATCAGGTAACGTCAACAGTGGTTGGTTCTGGTGCTACTAACTTTTCATTGTTAAAACAAGCAGGCGATGTTGCAGCTTAGTTGGTAATGAGGGTGTTGTCTTCGTTAACATCTATGCCAACGGTGGGATCTTCGGAGCTCACTTCACTAACTTTCACGAGGTCTCTGTTTATGGCTAGTCCATTGCAGTTGTCACCAACCAAGACCACGATGATTGAAAAGTAACAATTTTGTTCGTCCGCTACAACBsaXIPspXIkappa caseinp2a3,6803,7003,7203,7403,7603,7803,8003,8203,840
    AGGAAAATCCAGGACCTatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttTCCTTTTAGGTCCTGGAtactctaaaggaagttaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaaggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaaα-factor secretion signalα-factor secretion signalp2a3,8603,8803,9003,9203,9403,9603,9804,0004,020
    gccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctATGATGTCATTCGTATCTCTCTTACTGGTAGGGATACTATTTCATGCTACACAAGCCGAGCAATTAcggtaaaaggttgtcgtgtttattgcccaataacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgaTACTACAGTAAGCATAGAGAGAATGACCATCCCTATGATAAAGTACGATGTGTTCGGCTCGTTAATα-factor secretion signalalpha lactalbuminα-f...al4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ACAAAATGCGAAGTTTTTCAGGAACTTAAGGATTTAAAAGATTACGGTGGTGTGTCGTTACCAGAGTGGGTTTGTACCACCTTTCATACTTCAGGCTATGACACTCAGGCAATTGTTCAAAATAATGACAGTACTGAATATGGATTGTTTCAAATAAACAACAAAATATGGTGTATGTTTTACGCTTCAAAAAGTCCTTGAATTCCTAAATTTTCTAATGCCACCACACAGCAATGGTCTCACCCAAACATGGTGGAAAGTATGAAGTCCGATACTGTGAGTCCGTTAACAAGTTTTATTACTGTCATGACTTATACCTAACAAAGTTTATTTGTTGTTTTATACCACATAflIINmeAIIIalpha lactalbumin4,2204,2404,2604,2804,3004,3204,3404,360
    AGGACGACCAAAACCCTCATTCCAGAAATATTTGTAATATTTCTTGTGACAAGTTCTTAGATAATGATTTGACGGATGATATTATGTGCGTCAAAAAAATCCTGGATAAGGTCGGTATCAACTACTGGTTGGCGCACAAAGCTCTTTGCAGCGAAAAATTGGATCAATGGTTGTGTCCTGCTGGTTTTGGGAGTAAGGTCTTTATAAACATTATAAAGAACACTGTTCAAGAATCTATTACTAAACTGCCTACTATAATACACGCAGTTTTTTTAGGACCTATTCCAGCCATAGTTGATGACCAACCGCGTGTTTCGAGAAACGTCGCTTTTTAACCTAGTTACCAACACalpha lactalbumin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    TGAAAAGCTAGGTTCTGGTGCTACTAACTTTTCATTGTTAAAACAAGCAGGCGATGTTGAGGAAAATCCAGGACCTatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattACTTTTCGATCCAAGACCACGATGATTGAAAAGTAACAATTTTGTTCGTCCGCTACAACTCCTTTTAGGTCCTGGAtactctaaaggaagttaaaaatgacgacaaaataagcgtcgtaggaggcgtaatcgacgaggtcagttgtgatgttgtcttctactttgccgtgtttaap2aα-factor secretion signalα-factor secretion signalal...n4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggctgaagctATGAAGTggccgacttcgacagtagccaatgagtctaaatcttcccctaaagctacaacgacaaaacggtaaaaggttgtcgtgtttattgcccaataacaaatatttatgatgataacggtcgtaacgacgatttcttcttccccatagagagctcttttctctccgacttcgaTACTTCAα-factor secretion signalα-f...al4,7404,7604,7804,8004,8204,8404,8604,8804,900
    GTTTACTGATTACTTTAGGGCTCGCTTTAGCTTGCGGTGCTCAAGCCATTATAGTCACTCAGACAATGAAGGATCTGGACGTTCAAAAGGTGGCAGGCACATGGTACAGTTTGGCGATGGCAGCATCCGATATCTCGTTGCTAGATGCACAGTCTGCTCCGCTAAGGGTTTATGTCAAATGACTAATGAAATCCCGAGCGAAATCGAACGCCACGAGTTCGGTAATATCAGTGAGTCTGTTACTTCCTAGACCTGCAAGTTTTCCACCGTCCGTGTACCATGTCAAACCGCTACCGTCGTAGGCTATAGAGCAACGATCTACGTGTCAGACGAGGCGATTCCCAAATACASapIBspQIbeta lactoglobulin4,9204,9404,9604,9805,0005,0205,0405,060
    TGAAGAGCTAAAACCAACTCCAGGTGGTGACCTTGAAATTCTATTACAAAAGTGGGAAAATGGAAAGTGTGCTCAAAAAAAGATAATAGCAGAGAAAACAGAAATCCCTGCCGTCTTCAAGATCGACGCTTTAAACGAAAATAAAGTATTAGTCTTAGATACCGATTATAAAAAAACTTCTCGATTTTGGTTGAGGTCCACCACTGGAACTTTAAGATAATGTTTTCACCCTTTTACCTTTCACACGAGTTTTTTTCTATTATCGTCTCTTTTGTCTTTAGGGACGGCAGAAGTTCTAGCTGCGAAATTTGCTTTTATTTCATAATCAGAATCTATGGCTAATATTTTTTbeta lactoglobulin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    TACTTGCTTTTTTGTATGGAAAATTCTGCGGAACCTGAACAGTCACTGGCTTGCCAATGCTTGGTTAGAACCCCAGAGGTAGATGATGAAGCCATGGAAAAATTTGACAAAGCATTGAAAGCCCTTCCCATGCATATTAGATTGAGCTTCAACCCTACGCAATTGGAGGAGCAATATGAACGAAAAAACATACCTTTTAAGACGCCTTGGACTTGTCAGTGACCGAACGGTTACGAACCAATCTTGGGGTCTCCATCTACTACTTCGGTACCTTTTTAAACTGTTTCGTAACTTTCGGGAAGGGTACGTATAATCTAACTCGAAGTTGGGATGCGTTAACCTCCTCGTTAbeta lactoglobulin5,2605,2805,3005,3205,3405,3605,3805,4005,420
    GTCGTGTGcatggaacagaagttgatttccgaagaagacctcgagtaagcttggtaccgcggctagctaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaaCAGCACACgtaccttgtcttcaactaaaggcttcttctggagctcattcgaaccatggcgccgatcgattctaggcgagattggcttttccttcctcaatctgttggacttcagatccagggataaataaaaaaatatcaatacaatcataattcttgcaataaatataaagtttBanIBmtIKpnINheIPspXISacIIAcc65IHindIIICYC1 terminatorMyc5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctaaaaagaaaaaaaagacatgtctgcgcacatgcgtacattgtaatatgacttttggaacgaactcttccaaaaccctgcgagcttctaggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgaMluISapIBsaXIBspQIBsrGIPvuIICYC1 terminator5,6205,6405,6605,6805,7005,7205,7405,760
    gcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctcgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggaPciIori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatDrdIori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcApaLIBseYIori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    cagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaaori6,3206,3406,3606,3806,4006,4206,4406,460
    gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttaBspHIori6,4806,5006,5206,5406,5606,5806,6006,6206,640
    caatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggAhdIBanIBmrIBsaIAmpR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    gcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaaBglINmeAIIIAmpR6,8406,8606,8806,9006,9206,9406,9606,9807,000
    gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaatAmpR7,0207,0407,0607,0807,1007,1207,1407,160
    tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtaBsaHIAmpR7,1807,2007,2207,2407,2607,2807,3007,3207,340
    cattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcggtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgcApaLIAmpRAmpR7,3607,3807,4007,4207,4407,4607,4807,5007,520
    acacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgaacgaagcatctgtgcttcattgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggacttgcttcgtagacacgaagtaBspHIAmpR promoterAmpR2μ ori7,5407,5607,5807,6007,6207,6407,6607,6807,700
    tttgtagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaaaaacatcttgtttttacgttgcgctctcgcgattaaaaagtttgtttcttagactcgacgtaaaaatgtcttgtctttacgttgcgctttcgcgataaaatggttgcttcttagacacgaagtaaaaacattttgtttttacgttgcgctctcgcgattaaaaagtttgtttctt2μ ori7,7207,7407,7607,7807,8007,8207,8407,860
    tctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgaagactcgacgtaaaaatgtcttgtctttacgttgcgctctcgcgataaaatggttgtttcttagatatgaagaaaaaacaagatgtttttacgtagggctctcgcgataaaaagattgtttcgtagaatctaatgaaaaaaagaggaaacacgcgagatattacgtcagagaact2μ ori7,8807,9007,9207,9407,9607,9808,0008,0208,040
    taactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggatattgaaaaacgtgacatccaggcaattccaatcttcttccgatgaaaccacagataaaagagaaggtatttttttcggactgaggtgaagggcgcaaatgactaatgatcgcttcgacgcccacgtaaaaaagttctatttccgtaggggctaatataagatatggctacacctaAjuI2μ ori8,0608,0808,1008,1208,1408,1608,1808,2008,220
    tgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagacgcgtatgaaacacttgtctttcactatcgcaactactaagaagtaaccagtcttttaatacttgccaaagaagataaaacagagatatatgatgcatatcctttacaaatgtaaaagcataacaaaagctaagtgagatacttatcaagaatgatgttaaaaaaacagatttcBsaAISnaBI2μ ori8,2408,2608,2808,3008,3208,3408,3608,3808,400
    agtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttactcattatgatctctatttgtattttttacatctccagctcaaatctacgttcaagttcctcgctttccacctacccatccaatatatccctatatcgtgtctctatatatcgtttctctatgaaaactcgttacaaacaccttcgccataagcgttataaaatcatcgagcaatgCspCI2μ ori8,4208,4408,4608,4808,5008,5208,5408,560
    agtccggtgcgtttttggttttttgaaagtgcgtcttcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagagaataggaacttcggaataggaacttcaaagcgtttccgaaaacgagcgcttccgaaaatgcaacgcgagctgcgcacatacagctctcaggccacgcaaaaaccaaaaaactttcacgcagaagtctcgcgaaaaccaaaagttttcgcgagacttcaaggatatgaaagatctcttatccttgaagccttatccttgaagtttcgcaaaggcttttgctcgcgaaggcttttacgttgcgctcgacgcgtgtatgtcgagBarIXbaI2μ oriFRT8,5808,6008,6208,6408,6608,6808,7008,7208,740
    actgttcacgtcgcacctatatctgcgtgttgcctgtatatatatatacatgagaagaacggcatagtgcgtgtttatgcttaaatgcgtacttatatgcgtctatttatgtaggatgaaaggtagtctagtacctcctgtgatattatcccattccatgcggggtatcgtatgctgacaagtgcagcgtggatatagacgcacaacggacatatatatatatgtactcttcttgccgtatcacgcacaaatacgaatttacgcatgaatatacgcagataaatacatcctactttccatcagatcatggaggacactataatagggtaaggtacgccccatagcatacgBmgBI2μ ori8,7608,7808,8008,8208,8408,8608,8808,9008,920
    ttccttcagcactaccctttagctgttctatatgctgccactcctcaattggattagtctcatccttcaatgctatcatttcctttgatattggatcatggtagacaacccttaatataacttcgtataatgtatgctatacgaagttattaggtctagagatctgtttagcttgaaggaagtcgtgatgggaaatcgacaagatatacgacggtgaggagttaacctaatcagagtaggaagttacgatagtaaaggaaactataacctagtaccatctgttgggaattatattgaagcatattacatacgatatgcttcaataatccagatctctagacaaatcgaacBarIXbaIXcmIBglII2μ oriloxP8,9408,9608,9809,0009,0209,0409,0609,0809,100
    cctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagggagcaggggcggcccagtgggccggtcgctgtacctccgggtcttatgggaggaactgtcagaactgcacgcgtcgagtccccgtactacactgacagcgggcatgtaaatcgggtatgtaggggtacatattagtaaacgtaggtatgtaaaactaccggcgtgccgcgcttcBmgBITEF promoterkanMX9,1209,1409,1609,1809,2009,2209,2409,260
    caaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacgtttttaatgccgaggagcgacgtctggacgctcgtccctttgcgaggggagtgtctgcgcaacttaacaggggtgcggcgcggggacatctctttatattttccaatcctaaacggtgactccaagaagaaagtatatgaaggaaaattttagaacgatcctatgtcaagagtgMluIPstITEF promoterkanMX9,2809,3009,3209,3409,3609,3809,4009,4209,440
    atcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttagtgtaggcttgtatttgttggtacccattccttttctgagtgcaaagctccggcgctaatttaaggttgtacctacgactaaatatacccatatttacccgagcgctattacagcccgttagtccacgctgttagatagctaacatacccttcgggctacgcggtctcaacaaNruIkanMXKanRTEF promoter9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaaagactttgtaccgtttccatcgcaacggttactacaatgtctactctaccagtctgatttgaccgactgccttaaatacggagaaggctggtagttcgtaaaataggcatgaggactactacgtaccaatgagtggtgacgctaggggccgttttgtcgtaaggtccataatcttkanMXKanR9,6409,6609,6809,7009,7209,7409,7609,7809,800
    gaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagccttataggactaagtccacttttataacaactacgcgaccgtcacaaggacgcggccaacgtaagctaaggacaaacattaacaggaaaattgtcgctagcgcataaagcagagcgagtccgcgttagtgcttacttattgccaaaccaactacgctcactaaaactactgctcgAjuIAsiSIBsmBIEsp3IkanMXKanR9,8209,8409,8609,8809,9009,9209,9409,960
    gtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggacattaccgaccggacaacttgttcagacctttctttacgtattcgaaaacggtaagagtggcctaagtcagcagtgagtaccactaaagagtgaactattggaataaaaactgctcccctttaattatccaacataactacaacctgctcagccttagcgtctggctatggtcctHindIIIkanMXKanR9,98010,00010,02010,04010,06010,08010,10010,12010,140
    tcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagtactgacaataaaaagattcttgttttcaagaacttgtagaacggtaggataccttgacggagccactcaaaagaggaagtaatgtctttgccgaaaaagtttttataccataactattaggactatacttatttaacgtcaaagtaaactacgagctactcaaaaagattagtcatgactgttatttttctaagaacaaaagttcttgaacakanMXKanRTEF terminator10,16010,18010,20010,22010,24010,26010,28010,30010,320
    catttgtatagtttttttatattgtagttgttctattttaatcaaatgttagcgtgatttatattttttttcgcctcgacatcatctgcccagatgcgaagttaagtgcgcagaaagtaatatcatgcgtcaatcgtatgtgaatgctggtcgctatactgctgtcgattcgatagtaaacatatcaaaaaaatataacatcaacaagataaaattagtttacaatcgcactaaatataaaaaaaagcggagctgtagtagacgggtctacgcttcaattcacgcgtctttcattatagtacgcagttagcatacacttacgaccagcgatatgacgacagctaagctatkanMXTEF terminator10,34010,36010,38010,40010,42010,44010,46010,48010,500
    ctaacgccgccatccagtgtcgaaaacgagctctcgagaacccttaatataacttcgtataatgtatgctatacgaagttattaggtgatatcagatccactagattgcggcggtaggtcacagcttttgctcgagagctcttgggaattatattgaagcatattacatacgatatgcttcaataatccactatagtctaggtgatSacIEco53kIloxP10,52010,54010,56010,58010,600
    • Plasmid
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    • Linear Map
    Editing disabled on read-only sequence.
    10603 bp75008000850090009500100005001000150020002500beta caseinTEF terminatorADH1 terminator,FLAG,BstZ17I,PacI,Eco53kI,SacI,BglII,SpeI,EagI,NotIAhdI,BsaAI,PmlINgoMIVTEF1 promoter,EagI,NaeI,FseI,BplI,BsaI,BplIT7 promoter,dangly bits re-added back in,BamHI,PspOMI,TspMI,XmaI,ApaI,+4α-factor secretion signal,α-factor secretion signalα-factor secretion signalBmrINheIBmtI,HpaIAjuIBsaAI,SnaBI2μ ori,CspCIFRT,BarI,XbaI,BmgBIBarI,XcmIloxP,XbaI,BglII,BmgBITEF promoter,PstI,MluI,NruIkanMX,KanR,AsiSI,BsmBI,Esp3I,AjuIloxP,HindIII,Eco53kI,SacI

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    10603