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XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)
  • Sequence Map
    Editing disabled on read-only sequence.
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatgg*W286pUC ori20406080100120140160
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttBsaIHK284IL282SA280GI278EA276IQ274RN272RE270DM268TA266QS264GT262TY260IV258VI256RS254PK252GD250PG248LA246AI244IG242RS240GR238EG236AG234SK232DA230IF228171-1031Amp (bla)180200220240260280300320340
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaFspIWG226AP224LA222SR220LL218PG216AV214KD212AE210MW208DI206LQ204QR202SA200LT198LL196EG194TL192LK190RL188TT186AM184AV182PM180TT178DR176ED174NP172IA170171-1031Amp (bla)360380400420440460480500520
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattEN168LE166PE164WR162DL160RT158VH156DG154MN152HL150FA148TL146EK144PG142GI140TT138LL136LN134AA132TN130DS128MT126IA124AS122CL120ER118VT116MG114DT112L171-1031Amp (bla)540560580600620640660680700
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaBcgIH110KE108TV106PS104YE102VL100DN98QS96YH94IR92RG90LQ88EQ86GA84DI82RS80LV78AG76CL74LV72KF70TS68MM66PF64RE62EP60RF58SE56LI54K171-1031Amp (bla)720740760780800820840860
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIG52SN50LD48LE46IY44GV42RA40GL38QD36EA34DK32VK30VL28TE26PH24AF22VP20LC18FA16AF14FP12IL10AV8RF6HQ4IS2M171-1031Amp (bla)bla promoter8809009209409609801,0001,0201,040
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactBglIISgrDIbla promoter1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgccgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacggctattttattttctaaaataaatcagaggtctttttccccccttactttctggggtggacatccaMfeISFFV1,2401,2601,2801,3001,3201,3401,3601,3801,400
    tatggcaagctagctgcagtaacgccattattgcaaggcatggaaaaataccaaaccaagaatagagaagttcagatcaagggcgggtacatgaaaatagctaacgtagggccaaacaggatatctgcggtgagcagtttcggccccggcccggggccaagaacagatggtcaccataccgttcgatcgacgtcattgcggtaataacgttccgtacctttttatggtttggttcttatctcttcaagtctagttcccgcccatgtacttttatcgattgcatcccggtttgtcctatagacgccactcgtcaaagccggggccgggccccggttcttgtctaccagtggBmtINheIEcoRVSFFV1,4201,4401,4601,4801,5001,5201,5401,560
    gcagtttcggccccggcccgaggccaagagcagatggtccccagatatggcccaaccctcagcagtttcttaagacccatcagatgtttccaggctcccccaaggacctgaaatgaccctgcgccttatttgaattaaccaatcagcctgcttctcgcttctgttcgcgcgcttccgtcaaagccggggccgggctccggttctcgtctaccaggggtctataccgggttgggagtcgtcaaagaattctgggtagtctacaaaggtccgagggggttcctggactttactgggacgcggaataaacttaattggttagtcggacgaagagcgaagacaagcgcgcgaagAflIIBssHIISFFV1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tgcttcccgagctctataaaagagctcacaacccctcactcggcgcgccagtcctccgacagactgagtcgcccgggaagcttcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGacgaagggctcgagatattttctcgagtgttggggagtgagccgcgcggtcaggaggctgtctgactcagcgggcccttcgaagcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCAscIBssHIIVS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26N1844-2551M1mCherrySFFV1,7601,7801,8001,8201,8401,8601,8801,9001,920
    GCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATG28HE30FE32IE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY861844-2551mCherry1,9401,9601,9802,0002,0202,0402,0602,0802,100
    CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGAloISbfILK88LS90FP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT1441844-2551mCherry2,1202,1402,1602,1802,2002,2202,2402,260
    ATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAMG146WE148AS150SE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK2021844-2551mCherry2,2802,3002,3202,3402,3602,3802,4002,4202,440
    TGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTMluISgrAID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K222561-2659LD204IT206SH208NE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS2361844-2551mCherry5' StopGo (2A; Loughran 2017)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    GCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTBaeISpeIBamHIE1A2S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T372672-2782L23A24G25D26V27E28S29N30P31G32P332561-2659G1S2T1G2T1S2G1T2ADAR substrate3xMS2 SGR5' StopGo (2A; Loughran 2017)5' stem3' stem2,6402,6602,6802,7002,7202,7402,7602,7802,800
    CGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAACTTTTTACGGGCGTTGTTCCGATCCTCGTCGAATTGGACGGGGACGTTAATGGGCATAGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCACAGAGTTTCCCGCTCCTTGAAAAATGCCCGCAACAAGGCTAGGAGCAGCTTAACCTGCCCCTGCAATTACCCGTATPspXIVS2KG4EE6LF8TG10VV12PI14LV16EL18DG20DV22NG24H2900-3613R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P332795-2893S1S23' StopGo (2A; Loughran 2017)GFP2,8202,8402,8602,8802,9002,9202,9402,960
    AATTCTCAGTTAGTGGTGAGGGTGAGGGGGACGCGACATATGGGAAACTTACGCTGAAGTTCATATGTACCACCGGAAAATTGCCAGTGCCGTGGCCTACTCTCGTCACCACCCTGACATATGGTGTTCAGTGTTTTAGCCGGTATCCAGACCATATGAAACAGCACGATTTTTTTTAAGAGTCAATCACCACTCCCACTCCCCCTGCGCTGTATACCCTTTGAATGCGACTTCAAGTATACATGGTGGCCTTTTAACGGTCACGGCACCGGATGAGAGCAGTGGTGGGACTGTATACCACAAGTCACAAAATCGGCCATAGGTCTGGTATACTTTGTCGTGCTAAAAAAK26FS28VS30GE32GE34GD36AT38YG40KL42TL44KF46IC48TT50GK52LP54VP56WP58TL60VT62TL64TY66GV68QC70FS72RY74PD76HM78KQ80HD82FF842900-3613GFP2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TAAATCCGCTATGCCTGAAGGTTACGTACAGGAGCGAACGATATTTTTTAAAGACGACGGAAACTACAAAACTAGAGCCGAGGTAAAATTCGAGGGTGACACTCTCGTGAACCGGATCGAACTGAAGGGCATAGATTTTAAGGAGGATGGCAATATACTGGGCCACAAGCTCGAAATTTAGGCGATACGGACTTCCAATGCATGTCCTCGCTTGCTATAAAAAATTTCTGCTGCCTTTGATGTTTTGATCTCGGCTCCATTTTAAGCTCCCACTGTGAGAGCACTTGGCCTAGCTTGACTTCCCGTATCTAAAATTCCTCCTACCGTTATATGACCCGGTGTTCGAGCTTBarISnaBIKS86AM88PE90GY92VQ94ER96TI98FF100KD102DG104NY106KT108RA110EV112KF114EG116DT118LV120NR122IE124LK126GI128DF130KE132DG134NI136LG138HK140LE1422900-3613GFP3,1603,1803,2003,2203,2403,2603,2803,3003,320
    TACAACTATAATTCTCATAATGTCTATATCATGGCGGACAAACAGAAAAACGGCATCAAGGTAAACTTTAAAATCCGACACAATATTGAGGACGGCTCTGTCCAGCTTGCGGACCATTACCAACAGAACACGCCGATAGGGGATGGTCCAGTACTTCTCCCTGATAATCACTATTATGTTGATATTAAGAGTATTACAGATATAGTACCGCCTGTTTGTCTTTTTGCCGTAGTTCCATTTGAAATTTTAGGCTGTGTTATAACTCCTGCCGAGACAGGTCGAACGCCTGGTAATGGTTGTCTTGTGCGGCTATCCCCTACCAGGTCATGAAGAGGGACTATTAGTGATAASspIYN144YN146SH148NV150YI152MA154DK156QK158NG160IK162VN164FK166IR168HN170IE172DG174SV176QL178AD180HY182QQ184NT186PI188GD190GP192VL194LP196DN198HY2002900-3613GFP3,3403,3603,3803,4003,4203,4403,4603,4803,500
    TGTCTACTCAGTCAGCTCTTAGCAAGGACCCAAACGAAAAACGGGATCACATGGTCCTTCTTGAGTTCGTCACTGCGGCTGGGATAACACTCGGGATGGACGAACTGTATAAGtaggccggagggcccCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGACAGATGAGTCAGTCGAGAATCGTTCCTGGGTTTGCTTTTTGCCCTAGTGTACCAGGAAGAACTCAAGCAGTGACGCCGACCCTATTGTGAGCCCTACCTGCTTGACATATTCatccggcctcccgggGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCLS202TQ204SA206LS208KD210PN212EK214RD216HM218VL220LE222FV224TA226AG228IT230LG232MD234EL236YK2382900-3613*1GFPEMCV IRES SitePCRhomolog3,5203,5403,5603,5803,6003,6203,6403,660
    AATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGAAvrIIEMCV IRES Site3,6803,7003,7203,7403,7603,7803,8003,8203,840
    GGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCPmlIEMCV IRES Site3,8603,8803,9003,9203,9403,9603,9804,0004,020
    CTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTgCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATACCCTAGACTAGACCCCGGAGCCAcGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTAEMCV IRES Site4,0404,0604,0804,1004,1204,1404,1604,1804,200
    AATATGGCCACAACCATGaagacgatcatcgccctgagctacatcttctgcctggtattcgccgactacaaggacgatgatgacgccagcatcgatatgctcatggcctctaccacaagcgcCgtgcccggacatccatctcttccaagtcttccctccaacagctcacaggagaTTATACCGGTGTTGGTACttctgctagtagcgggactcgatgtagaagacggaccataagcggctgatgttcctgctactactgcggtcgtagctatacgagtaccggagatggtgttcgcgGcacgggcctgtaggtagagaaggttcagaagggaggttgtcgagtgtcctctMK2TI4IA6LS8YI10FC12LV14FA16DY18KD20DD22DA24SI26DM28LM30AS32TT34SA36VP38GH40PS42LP44SL46PS48NS50SQ52E4216-6726AVPR2Signal/FlagEMCV I... SitePCRIRESv0homologhomolog4,2204,2404,2604,2804,3004,3204,3404,360
    ggcctctggatacccgggacccacttcttgcaagagcggagctcgccttgctctccatcgttttcgttgccgtcgcgttgagcaatggtctggtgctggccgccctggcgcgacgaggccgccgaggccactgggcgcctattcatgtctttatcggccatctctgtcttgcagaccggagacctatgggccctgggtgaagaacgttctcgcctcgagcggaacgagaggtagcaaaagcaacggcagcgcaactcgttaccagaccacgaccggcgggaccgcgctgctccggcggctccggtgacccgcggataagtacagaaatagccggtagagacagaacgtctSfiIR54PL56DT58RD60PL62LA64RA66EL68AL70LS72IV74FV76AV78AL80SN82GL84VL86AA88LA90RR92GR94RG96HW98AP100IH102VF104IG106HL108CL110AD1124216-6726AVPR24,3804,4004,4204,4404,4604,4804,5004,5204,540
    cctggccgtggcgctgtttcaggtgctccctcaactggcttggaaagccaccgatagattccgggggccagacgcactgtgtcgggccgtgaaatacctgcagatggtgggaatgtatgccagttcatatatgatacttgctatgaccttggacagacacagggctatctgtcgcggaccggcaccgcgacaaagtccacgagggagttgaccgaacctttcggtggctatctaaggcccccggtctgcgtgacacagcccggcactttatggacgtctaccacccttacatacggtcaagtatatactatgaacgatactggaacctgtctgtgtcccgatagacagcgLA114VA116LF118QV120LP122QL124AW126KA128TD130RF132RG134PD136AL138CR140AV142KY144LQ146MV148GM150YA152SS154YM156IL158AM160TL162DR164HR166AI168CR1704216-6726AVPR24,5604,5804,6004,6204,6404,6604,6804,7004,720
    cccatgctggcgtataggcacggttccggagcccattggaacaggccagttctggttgcgtgggctttttccctgcttctgagcttgccccagttgtttattttcgcacagagaaatgtggagggcggatctggcgtgaccgattgctgggcttgcttcgctgagccatggggcagggtacgaccgcatatccgtgccaaggcctcgggtaaccttgtccggtcaagaccaacgcacccgaaaaagggacgaagactcgaacggggtcaacaaataaaagcgtgtctctttacacctcccgcctagaccgcactggctaacgacccgaacgaagcgactcggtaccccgtPM172LA174YR176HG178SG180AH182WN184RP186VL188VA190WA192FS194LL196LS198LP200QL202FI204FA206QR208NV210EG212GS214GV216TD218CW220AC222FA224EP226WG2284216-6726AVPR2homolog4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gaaggacctacgtgacgtggattgctctcatggtgttcgttgctcccaccctggggatcgccgcatgccaggtcctgatattcagagaaatacacgcctccttggttcctgggccaagcgagcgccctggtggaagaagacggggtcgaaggactggctctccaggcgaaggcgccttcctggatgcactgcacctaacgagagtaccacaagcaacgagggtgggacccctagcggcgtacggtccaggactataagtctctttatgtgcggaggaaccaaggacccggttcgctcgcgggaccaccttcttctgccccagcttcctgaccgagaggtccgcttccgcgPasIRR230TY232VT234WI236AL238MV240FV242AP244TL246GI248AA250CQ252VL254IF256RE258IH260AS262LV264PG266PS268ER270PG272GR274RR276GR278RT280GS282PG284EG286A4216-6726AVPR24,9204,9404,9604,9805,0005,0205,0405,060
    ccacgtatcagctgcggtagctaagaccgtacgcatgacactggtcatcgtcgtcgtttacgttctttgctgggccccgttttttttggtgcagctctgggcggcctgggaccccgaagcacccctggaaggtgctccattcgttctgctcatgcttctcgcttcactgaatagcggtgcatagtcgacgccatcgattctggcatgcgtactgtgaccagtagcagcagcaaatgcaagaaacgacccggggcaaaaaaaaccacgtcgagacccgccggaccctggggcttcgtggggaccttccacgaggtaagcaagacgagtacgaagagcgaagtgacttatcgBsiWIH288VS290AA292VA294KT296VR298MT300LV302IV304VV306YV308LC310WA312PF314FL316VQ318LW320AA322WD324PE326AP328LE330GA332PF334VL336LM338LL340AS342LN344S4216-6726AVPR25,0805,1005,1205,1405,1605,1805,2005,2205,240
    tgtaccaatccttggatttacgcctctttctcctccagtgttagctccgagctgcggagtcttctctgttgcgcccggggcaggacccccccaagcctgggtccacaggacgaatcctgcactaccgcctccagttccctggccaaagacacctcttctatcgataccggtggacacatggttaggaacctaaatgcggagaaagaggaggtcacaatcgaggctcgacgcctcagaagagacaacgcgggccccgtcctgggggggttcggacccaggtgtcctgcttaggacgtgatggcggaggtcaagggaccggtttctgtggagaagatagctatggccacctgC346TN348PW350IY352AS354FS356SS358VS360SE362LR364SL366LC368CA370RG372RT374PP376SL378GP380QD382ES384CT386TA388SS390SL392AK394DT396SS398ID400TG402G4216-6726AVPR25,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTCGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAEcoNIR404TP406PS408LG410PQ412DE414SC416TT418AS420SS422LA424KD426TS428SG430TG432AL434HL436DQ438TP440SR442QP444IP446SE448GL450QL452HL454PQ456VL458AD460AV4624216-6726hADAR2 cat domainAVPR2V2 tailhomologousfrom 2995,4405,4605,4805,5005,5205,5405,5605,5805,600
    CTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTASR464LV466LG468KF470GD472LT474DN476FS478SP480HA482RR484KV486LA488GV490VM492TT494GT496DV498KD500AK502VI504SV506ST508GT510KC512IN514GE516YM518SD5204216-6726hADAR2 cat domain5,6205,6405,6605,6805,7005,7205,7405,760
    CGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGRG522LA524LN526DC528HA530EI532IS534RR536SL538LR540FL542YT544QL546EL548YL550NN552KD554DQ556KR558SI560FQ562KS564ER566GG568FR570LK572EN574VQ576FH5784216-6726hADAR2 cat domain5,7805,8005,8205,8405,8605,8805,9005,9205,940
    TGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCTGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACBcgILY580IS582TS584PC586GD588AR590IF592SP594HE596PI598LE600EP602AD604RH606PN608RK610AR612GQ614LR616TK618IE620SG622QG624TI626PV628RS630NA632SI634QA636W4216-6726hADAR2 cat domain5,9605,9806,0006,0206,0406,0606,0806,1006,120
    GGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACBamHID638GV640LQ642GE644RL646LT648MS650CS652DK654IA656RW658NV660VG662IQ664GS666LL668SI670FV672EP674IY676FS678SI680IL682GS684LY686HG688DH690LS692RA694M4216-6726hADAR2 cat domainhomolog6,1406,1606,1806,2006,2206,2406,2606,2806,300
    TACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAAGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCY696QR698IS700NI702ED704LP706PL708YT710LN712KP714LL716SG718IS720NA722EA724RQ726PG728KA730PN732FS734VN736WT738VG740DS742AI744EV746IN748AT750TG752K4216-6726hADAR2 cat domain6,3206,3406,3606,3806,4006,4206,4406,460
    ATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGMluID754EL756GR758AS760RL762CK764HA766LY768CR770WM772RV774HG776KV778PS780HL782LR784SK786IT788KP790NV792YH794ES796KL798AA800KE802YQ804AA806KA808RL810FT8124216-6726hADAR2 cat domain6,4806,5006,5206,5406,5606,5806,6006,6206,640
    AGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGATAGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGACTATCGGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCTTCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGAF814IK816AG818LG820AW822VE824KP826TE828QD830QF832SL834TP836*4216-6726EMCV IRES SitehADAR2 cat domainhomologPCR6,6606,6806,7006,7206,7406,7606,7806,8006,820
    GTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCAGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCCCCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAAGGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCAvrIIEMCV IRES Site6,8406,8606,8806,9006,9206,9406,9606,9807,000
    CGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACGCCTTGGGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGTTTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACCGAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGPmlIEMCV IRES Site7,0207,0407,0607,0807,1007,1207,1407,160
    CCCATTGTATGGGATCTGATCTGGGGCCTCGGTaCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACAACCATGggggagaaacccgggaccagggtcttcaagaGGGTAACATACCCTAGACTAGACCCCGGAGCCAtGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTTGCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATACCGGTGTTGGTACcccctctttgggccctggtcccagaagttctMG2EK4PG6TR8VF10K7317-9287EMCV IRES SiteARRB27,1807,2007,2207,2407,2607,2807,3007,3207,340
    agtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccctgtagatggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctatggccgtgaagacctggatcagctcgggattgacgttcgagtggcacatgaacccgttcgccctgaagcatctagtggacctgtttcacctgggacatctaccgcaccacgaacacctgggactgatggacttcctggcgtttcacaaacactgggagtggacgcggaaggcgataccggcacttctggacctK12SS14PN16CK18LT20VY22LG24KR26DF28VD30HL32DK34VD36PV38DG40VV42LV44DP46DY48LK50DR52KV54FV56TL58TC60AF62RY64GR66ED68LD707317-9287ARRB27,3607,3807,4007,4207,4407,4607,4807,5007,520
    tgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccccggccccccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccagaatcttccatgctccgtcacacgacccgaacaggaaggcgtttctggacaagtagcggtggatggtccggaaggggggccacgggttgggtggggccggggggtgggcggacgtcctggccgacgactccttcgacccggtcgtacgggtggggaagaagaagtggtatggggtcttagaaggtacgaggcagSbfIVL72GL74SF76RK78DL80FI82AT84YQ86AF88PP90VP92NP94PR96PP98TR100LQ102DR104LL106RK108LG110QH112AH114PF116FF118TI120PQ122NL124PC126SV1287317-9287ARRB27,5407,5607,5807,6007,6207,6407,6607,6807,700
    acactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaatcactagaagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggcccccagccttcagccgaaatgtgacgtcggtccgggtctcctatgtcctttccggacgccgcatctgaaactctaagctcggaagacacgatttagtgatcttctcttttcggtgttttccttgagacacgccgaccactaggctttccacgtcaagcggggcctctttgggccgggggtcggaagtcggctttTL130QP132GP134ED136TG138KA140CG142VD144FE146IR148AF150CA152KS154LE156EK158SH160KR162NS164VR166LV168IR170KV172QF174AP176EK178PG180PQ182PS184AE1867317-9287ARRB27,7207,7407,7607,7807,8007,8207,8407,860
    ccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatggggagcccctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtacgccgacatctgcctcttggtgtgcggtgaaggagtacagactggccagggacgtggagctccgaagggacctgttcctcgacatgatggtacccctcggggagttacatttacaggtgcagtggttgttgaggtggttctggcagttcttctagtttcagagacactctgtcatgcggctgtagacggagaaEcoNIPspXITT188RH190FL192MS194DR196SL198HL200EA202SL204DK206EL208YY210HG212EP214LN216VN218VH220VT222NN224ST226KT228VK230KI232KV234SV236RQ238YA240DI242CL244F7317-9287ARRB27,8807,9007,9207,9407,9607,9808,0008,0208,040
    cagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgtaaggtgtacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgaggacaccaacctggcttccgtcgtggcgggtcatgttcacaggacaccgagttgagcttgttctactggtccatagagggtcgaggtgtaagacattccacatgtggtattggggtgacgagtcgctgttggccctcttcgccccagagcgggacctaccctttgagttcgtgctcctgtggttggaccgaaggBsaISexAIS246TA248QY250KC252PV254AQ256LE258QD260DQ262VS264PS266ST268FC270KV272YT274IT276PL278LS280DN282RE284KR286GL288AL290DG292KL294KH296ED298TN300LA302S7317-9287ARRB2homolog8,0608,0808,1008,1208,1408,1608,1808,2008,220
    agcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctggtggtgtctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccccagaccccagtcagccgtcgtggtagcacttcctcccacggttgttcctccacgacccttaggaccacaggatgtcccagttccacttcgaccaccacagagctccgcccctacagagacacctcgacggaaaacaagaatacgtggggttcggggtgctggtgtagggggaggggtctggggtcagtcggcS304TI306VK308EG310AN312KE314VL316GI318LV320SY322RV324KV326KL328VV330SR332GG334DV336SV338EL340PF342VL344MH346PK348PH350DH352IP354LP356RP358QS360A7317-9287ARRB28,2408,2608,2808,3008,3208,3408,3608,3808,400
    ctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgtgtttgaggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggcGGTGGTAGCGCTATGGCGAGCAACTTTACgaggcctctgtctacagggacacctgtggttggagtaacttaaactatggttgatacggtgtctactactgtaacacaaactcctgaaacgggccgaagccgacttcccctacttcctactgctgatactactagttgagacgccgCCACCATCGCGATACCGCTCGTTGAAATGAfeIA362PE364TD366VP368VD370TN372LI374EF376DT378NY380AT382DD384DI386VF388ED390FA392RL394RL396KG398MK400DD402DY404DD406QL408CG410GG412SA414MA416SN418FT4207317-9287ARRB2MCPfrom ...dMCPhomolog8,4208,4408,4608,4808,5008,5208,5408,560
    GCAATTTGTGTTGGTTGACAATGGTGGCACCGGAGACGTAACTGTTGCGCCGAGCAATTTTGCGAACGGGATTGCAGAATGGATAAGTAGTAACAGCCGGAGTCAGGCATATAAAGTCACATGCTCAGTTCGCCAAAGTTCCGCACAGAACCGAAAATATACCATAAAGGTAGAACGTTAAACACAACCAACTGTTACCACCGTGGCCTCTGCATTGACAACGCGGCTCGTTAAAACGCTTGCCCTAACGTCTTACCTATTCATCATTGTCGGCCTCAGTCCGTATATTTCAGTGTACGAGTCAAGCGGTTTCAAGGCGTGTCTTGGCTTTTATATGGTATTTCCATCTTQF422VL424VD426NG428GT430GD432VT434VA436PS438NF440AN442GI444AE446WI448SS450NS452RS454QA456YK458VT460CS462VR464QS466SA468QN470RK472YT474IK476VE4787317-9287MCPfrom pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCP8,5808,6008,6208,6408,6608,6808,7008,7208,740
    GTGCCAAAAGGGGCGTGGCGATCTTATCTTAATATGGAGCTTACGATCCCAATCTTTGCGACTAATTCAGACTGCGAGCTGATTGTCAAGGCCATGCAAGGTCTTTTGAAAGACGGTAACCCGATTCCCTCTGCGATCGCCGCCAACTCAGGAATATATGGTGGCGGAACCGGTGCACGGTTTTCCCCGCACCGCTAGAATAGAATTATACCTCGAATGCTAGGGTTAGAAACGCTGATTAAGTCTGACGCTCGACTAACAGTTCCGGTACGTTCCAGAAAACTTTCTGCCATTGGGCTAAGGGAGACGCTAGCGGCGGTTGAGTCCTTATATACCACCGCCTTGGCCACAsiSIBsaBIVP480KG482AW484RS486YL488NM490EL492TI494PI496FA498TN500SD502CE504LI506VK508AM510QG512LL514KD516GN518PI520PS522AI524AA526NS528GI530YG532GG534TG5367317-9287from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP8,7608,7808,8008,8208,8408,8608,8808,9008,920
    GCGGGTCCATGGCCTCCAATTTTACACAGTTTGTGCTCGTGGACAACGGCGGCACTGGAGACGTCACAGTAGCTCCAAGTAACTTTGCGAATGGGATAGCCGAGTGGATCTCTTCCAATTCAAGATCACAAGCCTATAAGGTGACTTGTAGTGTACGCCAATCTAGTGCCCAGAACGCCCAGGTACCGGAGGTTAAAATGTGTCAAACACGAGCACCTGTTGCCGCCGTGACCTCTGCAGTGTCATCGAGGTTCATTGAAACGCTTACCCTATCGGCTCACCTAGAGAAGGTTAAGTTCTAGTGTTCGGATATTCCACTGAACATCACATGCGGTTAGATCACGGGTCTTGG538SM540AS542NF544TQ546FV548LV550DN552GG554TG556DV558TV560AP562SN564FA566NG568IA570EW572IS574SN576SR578SQ580AY582KV584TC586SV588RQ590SS592AQ594N7317-9287from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCP8,9408,9608,9809,0009,0209,0409,0609,0809,100
    CCGGAAGTATACCATTAAAGTGGAGGTCCCCAAAGGTGCATGGCGCTCATACTTGAATATGGAATTGACGATTCCGATATTTGCGACCAATAGCGACTGCGAGCTTATTGTAAAAGCCATGCAAGGACTTCTGAAAGATGGCAACCCGATACCTTCCGCAATCGCTGCGAATAGCGGCCTTCATATGGTAATTTCACCTCCAGGGGTTTCCACGTACCGCGAGTATGAACTTATACCTTAACTGCTAAGGCTATAAACGCTGGTTATCGCTGACGCTCGAATAACATTTTCGGTACGTTCCTGAAGACTTTCTACCGTTGGGCTATGGAAGGCGTTAGCGACGCTTATCGBstZ17IR596KY598TI600KV602EV604PK606GA608WR610SY612LN614ME616LT618IP620IF622AT624NS626DC628EL630IV632KA634MQ636GL638LK640DG642NP644IP646SA648IA650AN652S7317-9287from pCDH 3FLAG-ADAR1-p110-HIS_kozak_tdMCPMCPPCR9,1209,1409,1609,1809,2009,2209,2409,260
    GGAATTTACtgaTTCGAAggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctCCTTAAATGactAAGCTTccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaPmeIBstBIG654IY656*7317-9287BGH polyadenylation sequencec-myc epitopePolyhi...e taghomologPCRMCP9,2809,3009,3209,3409,3609,3809,4009,4209,440
    ggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctBglIIBGH polyadenylation sequence9,4609,4809,5009,5209,5409,5609,5809,6009,620
    tgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaBGH pA9,6409,6609,6809,7009,7209,7409,7609,7809,800
    tcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatNaeIXbaINgoMIVFRT9,8209,8409,8609,8809,9009,9209,9409,960
    ggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggcBarIHygromycinFRT9,98010,00010,02010,04010,06010,08010,10010,12010,140
    atggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcataccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtEcoRIHygromycin10,16010,18010,20010,22010,24010,26010,28010,30010,320
    gccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcaAsiSIRsrIIHygromycin10,34010,36010,38010,40010,42010,44010,46010,48010,500
    gcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgcgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcHygromycin10,52010,54010,56010,58010,60010,62010,64010,660
    ccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacAjuISacIIHygromycin10,68010,70010,72010,74010,76010,78010,80010,82010,840
    ggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgaccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctHygromycin10,86010,88010,90010,92010,94010,96010,98011,00011,020
    gatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcactaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtNaeIPsiINgoMIVSV40 pA11,04011,06011,08011,10011,12011,14011,16011,18011,200
    caaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgggtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccBstZ17ISV40 pA11,22011,24011,26011,28011,30011,32011,34011,360
    aagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagSapIBspQI11,38011,40011,42011,44011,46011,48011,50011,52011,540
    actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgpUC ori11,56011,58011,60011,62011,64011,66011,68011,70011,720
    tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgpUC ori11,74011,76011,78011,80011,82011,84011,86011,88011,900
    gctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccapUC ori11,92011,94011,96011,98012,00012,02012,04012,060
    aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgttgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgcpUC ori12,08012,10012,12012,14012,16012,18012,20012,22012,240
    gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori12,26012,28012,300
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12303 bp90001000011000100020003000GFPPCR3' stemmutation siteopposite edited AG->A7317-92872900-3613EKo326.2 (DNA)EKo309.1 (DNA)Cit_1 (DNA)XZp042.5 (DNA)pNSK019 (DNA)EKo480.3 (DNA)SB004.3 (DNA)SB004.4 (DNA)YXo001.1 (DNA)XZp025.3 (DNA)XZp050.3 (DNA)XZp050.4 (DNA)XZp079.5 (DNA)XZp086.2 (DNA)BGHR Genewiz (DNA)EKo326.3 (DNA)M13-48REV Genewiz (DNA)EKo152.2 (DNA),BsaIAmp (bla),171-1031,FspIBcgIbla promoter,SspIFlpInF1 (DNA),SgrDI,BglIImissing in pSLQ2349,FlpInF (DNA),EKo285.1 (DNA),+5missing in pSLQ2349,A->T from pSLQ2349,NheI,BmtI,+1SFFV,G->A from pSLQ2349,AflIIEK_SFFV_F2 (DNA),BssHIIKozak,1841-1843,EKo271.2 (DNA),XZp054.1 (DNA),+7Che_8 (DNA)EKo230.1 (DNA),mChe_4 (DNA),mChe_1 (DNA)mChe_3 (DNA),pNSK093 (DNA)SbfImCherry,1844-2551,Che_6 (DNA),AloINK-cherry-seq (DNA),NK-cherry-seq (DNA),+1mChe_2 (DNA),Che_7 (DNA),+65' StopGo (2A; Loughran 2017),+85' stem,ADAR substrate,edited,+133xMS2 SGR,loop MS2,loop MS2,bulged,+123' StopGo (2A; Loughran 2017),+6EKo147.1 (DNA),GFP5 (DNA),+1pNSK084 (DNA)MCP-ADAR-seq-F0 (DNA)BstBI cut site,homolog,+5c-myc epitope,New Stop,+5NK-triggerEK0300-Sanger-r (DNA),+2BGH polyadenylation sequenceBGH pA,bghF (DNA),EKo293.3 (DNA),BglIIFlpInR (DNA)NgoMIV,NaeIFrtF (DNA)FRTXbaIEKo299.3 (DNA),BarIEKo152.1 (DNA),EcoRIAsiSI,RsrIIHygromycinAjuISacIIHyg_F (DNA)SV40PA_R (DNA),NgoMIV,NaeI,PsiISV40 pA,SV40pA-R Genewiz (DNA),M13R Genewiz (DNA),BstZ17IBspQI,SapIpUCori5F (DNA),pUCoriR (DNA)pUC ori

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    12303

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