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  • 0 feature results
plNP466 (pMtbWhiB7:mScarlet)
  • Sequence Map
    Editing disabled on read-only sequence.
    CGACCACTCGGCGTACAGCTCGTCCAGGCCGCGCACCCACACCCAGGCCAGGGTGTTGTCCGGCACCACCTGGTCCTGGACCGCGCTGATGAACAGGGTCACGTCGTCCCGGACCACACCGGCGAAGTCGTCCTCCACGAAGTCCCGGGAGAACCCGAGCCGGTCGGTCCAGAACGCTGGTGAGCCGCATGTCGAGCAGGTCCGGCGCGTGGGTGTGGGTCCGGTCCCACAACAGGCCGTGGTGGACCAGGACCTGGCGCGACTACTTGTCCCAGTGCAGCAGGGCCTGGTGTGGCCGCTTCAGCAGGAGGTGCTTCAGGGCCCTCTTGGGCTCGGCCAGCCAGGTCTTGSexAISgrAIble (zeoR)20406080100120140160
    TCGACCGCTCCGGCGACGTCGCGCGCGGTGAGCACCGGAACGGCACTGGTCAACTTGGCCATGGTTTAGTTCCTCACCTTGTCGTATTATACTATGCCGATATACTATGCCGATGATTAATTGTCAACACCTGCAGGTCGAGATTTACAGTCGGTCGCCTCCGGCCGACGGCGGTAGCTGGCGAGGCCGCTGCAGCGCGCGCCACTCGTGGCCTTGCCGTGACCAGTTGAACCGGTACCAAATCAAGGAGTGGAACAGCATAATATGATACGGCTATATGATACGGCTACTAATTAACAGTTGTGGACGTCCAGCTCTAAATGTCAGCCAGCGGAGGCCGGCTGCCGCCABcgINcoIPstISbfIZraIAatIIMauBIPaqCIEM7 promoterble (zeoR)180200220240260280300320340
    GCGGGCCCGGCCGGCCGCGATCGCCGCCGACCTACACGTATTCGCAGAGTTAAGCGGTCACCCCCGCCGGTTTGACCCCAATTTTGACCCCAATTGCATCGGGGTTCGGGGCGATTCGCCGATGTCCGCCATTGTGCGCAACCGGGCGTGACCTGCGGATTCGGTACCCGGGCGGCGCCCGGGCCGGCCGGCGCTAGCGGCGGCTGGATGTGCATAAGCGTCTCAATTCGCCAGTGGGGGCGGCCAAACTGGGGTTAAAACTGGGGTTAACGTAGCCCCAAGCCCCGCTAAGCGGCTACAGGCGGTAACACGCGTTGGCCCGCACTGGACGCCTAAGCCATGGGCCCGCCApaIBglIFseIFspIKpnIMfeIPvuISfiIAsiSIAcc65IPspOMIattPattP360380400420440460480500520
    ATCCACGCGGATGCCTTTTGAATGACTGGGGGTCAAGGGGTCGCAGGTTCAAATCCTGTCAGCCCGACGCAGATCAGGGGCGGTTTTCCGGAGACGGGAAGCCGCCCCTTTTTCTGTTTGACCCCAATTTTGACCCCAATTGCTCCGGCGTCCGTCCGCGTCCGTCGAGCTCGCTTAGGTGCGCCTACGGAAAACTTACTGACCCCCAGTTCCCCAGCGTCCAAGTTTAGGACAGTCGGGCTGCGTCTAGTCCCCGCCAAAAGGCCTCTGCCCTTCGGCGGGGAAAAAGACAAACTGGGGTTAAAACTGGGGTTAACGAGGCCGCAGGCAGGCGCAGGCAGCTCGAGCGAMfeISacIBsmBIBspEIEsp3IEco53kIattPattP540560580600620640660680700
    AAGACTGTCCGCTGCCGTCCGCGATCCGCAGGCGCGCCGACAACACGTCGACCATTGCGGTGTCCTCTCGGGGGTCGCCGGGTGCGTGGGGATCCACGCCGAATAGTGCCCGGTCGGGATAGGTGTACGGTCGCCTCCGGTCACGTCCGGGCGTCCGCGTACGCCGATTGTGTCATTCTGACAGGCGACGGCAGGCGCTAGGCGTCCGCGCGGCTGTTGTGCAGCTGGTAACGCCACAGGAGAGCCCCCAGCGGCCCACGCACCCCTAGGTGCGGCTTATCACGGGCCAGCCCTATCCACATGCCAGCGGAGGCCAGTGCAGGCCCGCAGGCGCATGCGGCTAACACAGTAccIAscISalIBsiWIBsrDI'int-giles720740760780800820840860
    CCGCGGCGATCATCATTCCAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAGGCGCCGCTAGTAGTAAGGTTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTBspHI8809009209409609801,0001,0201,040
    AAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATT1,0601,0801,1001,1201,1401,1601,1801,2001,220
    TCCTGTTACCAGTGGCTGATCGATTTAatGGGTCTGACGCTCATGGAACAATAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTATCTGTTGTTTGAAAAAAAAAAGCCCCGCGATTGCGGGGCTTTTTTTTTTCATATGtatcaCTTAAGattcaaatactagtgttTAGGACAATGGTCACCGACTAGCTAAATtaCCCAGACTGCGAGTACCTTGTTATTTGCTTTCCGAGTCAGCTTTCTGACCCGGAAAGCAAATAGACAACAAACTTTTTTTTTTCGGGGCGCTAACGCCCCGAAAAAAAAAAGTATACatagtGAATTCtaagtttatgatcacaaAClaISpeIAflIIBspDIAmplicon plJR876insertrnBT1 (hairpin -15.8 kcal/mol)TsynA (hairpin -15 kcal/mol)Amplicon plJR9623292olNP593olNP7681825olNP6561,2401,2601,2801,3001,3201,3401,3601,3801,400
    TActtatagagttcatccatgccgccggtggagtgacgcccctcggaacgctcgtattgctcgaccacggtgtagtcctcattatgggaggtgatgtccagtttccgatcgacattgtaggcccccggcatttgcacgggtttcttcgccttataggtggtcttgaaatcggccaATgaatatctcaagtaggtacggcggccacctcactgcggggagccttgcgagcataacgagctggtgccacatcaggagtaataccctccactacaggtcaaaggctagctgtaacatccgggggccgtaaacgtgcccaaagaagcggaatatccaccagaactttagccggtPvuIXcmIBsaXISgrAI*K232YL230ED228MG226GT224SH222RG220ES218RE216YQ214EV212VT210YD208EN206HS204TI202DL200KR198DV196NY194AG192PM190QV188PK186KA184KY182TT180KF178DA176L1400-2098Amplicon plJR962mScarlet-1mScarletolNP7681,4201,4401,4601,4801,5001,5201,5401,560
    agtagcggcccccgtctttcaaacgcagcgccattttgatatcgcccttcaagacgccatcttcggggtagagacgctccgtggacgcctcccaacccatcgtcttcttctgcatcaccggaccgtctggggggaaattggtgccacgcagtttgaccttatagatgagggtacctcatcgccgggggcagaaagtttgcgtcgcggtaaaactatagcgggaagttctgcggtagaagccccatctctgcgaggcacctgcggagggttgggtagcagaagaagacgtagtggcctggcagacccccctttaaccacggtgcgtcaaactggaatatctactcccatggBbsIKpnIBsmBICspCIEcoRVEsp3IRsrIIAcc65IY174RG172GD170KL168RL166AM164KI162DG160KL158VG156DE154PY152LR150ET148SA146EW144GM142TK140KQ138MV136PG134DP132PF130NT128GR126LK124VK122YI120LT118G1400-2098Amplicon plJR962mScarlet-1mScarlet1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gtcctccaaggacgtgtcctgggtcaccgtgaccgcaccgccatcctcgaaattcatgacgcgttcccacttaaaaccctctgggaagctctgcttgtaatagtctgggatatccgctgggtgtttgatgaaggcccgggacccatacatgaactgtggggacaggatgtcccagcaggaggttcctgcacaggacccagtggcactggcgtggcggtaggagctttaagtactgcgcaagggtgaattttgggagacccttcgagacgaacattatcagaccctataggcgacccacaaactacttccgggccctgggtatgtacttgacacccctgtcctacagggtcKflIMluIBspHIEcoRVD116EL114ST112DQ110TV108TV106AG104GD102EF100NM98VR96EW94KF92GE90PF88SQ86KY84YD82PI80DA78PH76KI74FA72RS70GY68MF66QP64SL62ID60W1400-2098Amplicon plJR962mScarlet-1mScarlet1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ctaaatggcaagggaccacctttggtcaccttcagtttggcggtttgcgtaccctcatatgggcggccctcaccctccccttcgatttcaaactcatggccgttcatggacccctccatatgcactttaaaccgcataaattctttaatcacggcttcgcccttggacaccatgtgatttaccgttccctggtggaaaccagtggaagtcaaaccgccaaacgcatgggagtatacccgccgggagtgggaggggaagctaaagtttgagtaccggcaagtacctggggaggtatacgtgaaatttggcgtatttaagaaattagtgccgaagcgggaacctgtggtacaDraIBstXIS58FP56LP54GG52KT50VK48LK46AT44QT42GE40YP38RG36EG34EG32EI30EF28EH26GN24MS22GE20MH18VK16FR14MF12EK10IV8AE6GK4SV2M1400-2098Amplicon plJR962mScarlet-1mScarletolNP773olNP7741,9401,9601,9802,0002,0202,0402,0602,0802,100
    cctgtttcacctgcttcctggtctggtggcggttcttcgaaagtgatccggaccagggatgctgcggtgggcagatgtcccgaaagtttggccacggatcctgtgacttcgggtccgtggccatctggcgaaacggggctgattacgtagcgcttacgtagagccccgctccacgggacaaagtggacgaaggaccagaccaccgccaagaagctttcactaggcctggtccctacgacgccacccgtctacagggctttcaaaccggtgcctaggacactgaagcccaggcaccggtagaccgctttgccccgactaatgcatcgcgaatgcatctcggggcgaggtgcAfeIBplIBspEIBstBIPaqCISnaBISnaBIAmplicon plJR962Mtb whiB7 promoter (+500)olNP774olNP7732,1202,1402,1602,1802,2002,2202,2402,260
    gactcgtcagtcgcggcggcgacacggttcttgctatggggggttcccgcggttggcaccgcggcagccgcgccgacaccaaatgcgttgttgtcaatcaccgcggccgccctcctctcgtgtcgcgcgcggttgccagccccccaatgccatctccaggctggcagcagaatgcctgagcagtcagcgccgccgctgtgccaagaacgataccccccaagggcgccaaccgtggcgccgtcggcgcggctgtggtttacgcaacaacagttagtggcgccggcgggaggagagcacagcgcgcgccaacggtcggggggttacggtagaggtccgaccgtcgtcttacgBplIBpmIDrdINotIXcmIBseRIBssSIMauBIBssSαIAmplicon plJR962Mtb whiB7 promoter (+500)olNP8112,2802,3002,3202,3402,3602,3802,4002,4202,440
    gacctggaggttaaccggtggcagcagctgaccacaaccgattttctgacctgcgcgtttgccggtacaggcccggttcaggtccgaccgcgaaccagctgcagcacgtccgatccgtattgttccagcttgcgggcaccgatgccggaactatttaattggggaccctagaggtctggacctccaattggccaccgtcgtcgactggtgttggctaaaagactggacgcgcaaacggccatgtccgggccaagtccaggctggcgcttggtcgacgtcgtgcaggctaggcataacaaggtcgaacgcccgtggctacggccttgataaattaacccctgggatctccaAgeIBcgIBpmIBsmIHpaIKflIPstIPvuIIPvuIIAmplicon plJR962Mtb whiB7 promoter (+500)olNP775olNP590olNP662T4_32 term2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccccttttttattttaaattaattagctaaagcgctagcccatggtcaTGGAAACCGTTGTGGTCTCCCTATAGTGAGTCGTATTAATTTACGCGTTTCACTGGCCGATTGCTCACACATGAACAAAGCAGTAGAGTGTCACTTTCAGCCCTCTTATCCTCGGCGATGTGTGTAAggggaaaaaataaaatttaattaatcgatttcgcgatcgggtaccagtACCTTTGGCAACACCAGAGGGATATCACTCAGCATAATTAAATGCGCAAAGTGACCGGCTAACGAGTGTGTACTTGTTTCGTCATCTCACAGTGAAAGTCGGGAGAATAGGAGCCGCTACACACATTAfeIBmtIBsaIDraIMluINcoINheIBstXIgbRL16_barcodeLibAmplicon plJR962105bpUpstream_RandomGC50GibsonHandleqtag19T7 promotergBlock handleBackbone_handleT4_32 term2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AATGGCGTTGATCTGGATTGACTCTATGACGGTATCTGGAATTCCGANNNNNNNNNNNNNNNNNNGaattcCTTGTAGGTGTGGAATCACTTAGCTTCGCGCCGAAGTCTTATGGCAAAACCGATGGACAATGATTCGGGTAGCACTAAAAGTCCATAGCACGTGCATCCCAACGTTACCGCAACTAGACCTAACTGAGATACTGCCATAGACCTTAAGGCTNNNNNNNNNNNNNNNNNNCttaagGAACATCCACACCTTAGTGAATCGAAGCGCGGCTTCAGAATACCGTTTTGGCTACCTGTTACTAAGCCCATCGTGATTTTCAGGTATCGTGCACGTAGGGTTGCAclIPmlIBtgZIEcoRIEcoRIgbRL16_barcodeLib105bpDownstream_RandomGC50105bpUpstream_RandomGC50barcodeFromNateHicksRandom 18-merGibsonHandle2,8202,8402,8602,8802,9002,9202,9402,960
    TTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGAACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACDrdIBssSIBssSαIoriEgbRL16_...odeLibGibsonHandle2,9803,0003,0203,0403,0603,0803,1003,1203,140
    CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTTACGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAApaLIBciVIoriE3,1603,1803,2003,2203,2403,2603,2803,3003,320
    CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGAoriE3,3403,3603,3803,4003,4203,4403,4603,4803,500
    GAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACTAGTCGCAACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGATCAGCGTTSpeIoriE3,5203,5403,5603,5803,6003,6203,6403,660
    CGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAGATGCATTCAGTCCTGCTCCTCGGCCACGAAGGCAAGTTTAGGCGAGGGCCGCCTAAACAGGATGAGTCCTCTCGCAAGTGGCTGTTTGTTGTCTATTTTGCTTTCCGGGTCAGAAAGCTGACTCGGAAAGCAAAATAAACTACGGACCGTCAAGGGATGAGAGCGTACCCCTCTCTACGTAAGTCAGGACGAGGAGCCGGTGCTTCAclIBsmINsiIBseRIDraIIITrrnBrrnBble (zeoR)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    TGCACGCAGTTGCCGGCCGGGTCGCGCAGGGCGAACTCCCGCCCCCACGGCTGCTCGCCGATCTCGGTCATGGCCGGCCCGGAGGCGTCCCGGAAGTTCGTGGACACGACCTCACGTGCGTCAACGGCCGGCCCAGCGCGTCCCGCTTGAGGGCGGGGGTGCCGACGAGCGGCTAGAGCCAGTACCGGCCGGGCCTCCGCAGGGCCTTCAAGCACCTGTGCTGGAGBglIFseIApaLIBsaXIble (zeoR)3,8603,8803,9003,9203,9403,960
    • Plasmid
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    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    3963 bp27503000325035003750250500750GibsonHandleoriE'int-gilesattPattPrrnBolJR1851 (DNA)olJR1691 (DNA)olJR1692 (DNA)ble (zeoR),SexAI,SgrAIEM7 promoter,ZraI,AatII,MauBI,NcoIPstI,SbfI,PaqCI,BcgI,PspOMI,ApaI,BglI,SfiI,FseIAsiSI,PvuIMfeI,FspIAcc65IKpnIBsmBI,Esp3I,BspEIMfeI,Eco53kI,SacIAscISalIAccI,BsrDIBsiWIBspHIAclIolKE318(libAmpRev) (DNA)DrdIBssSIBssSαIBciVIApaLISpeITrrnB,AclIBsmI,NsiI,BseRI,DraIII,ApaLI,BglI,FseI,BsaXI

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    3963