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XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcccaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggSpeICMVCMV promoter (with TetOs)20406080100120140160
    aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    ccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcBsaBIBsaBISgrDICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    aagacaccgggaccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaaBsmINotIPmeIAflIISacIITEV leader UTR55' IVT primerT7 insertCMV promot...th TetOs)homolog720740760780800820840860
    aaatcatttcttttaaagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCtttagtaaagaaaatttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGAleIMscISwaIXmnIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32I952-1659mCherryTEV leader UTR5homolog8809009209409609801,0001,0201,040
    GAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCTCCCGCTCCCGCTCCCGGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGBstEIIE34GE36GE38GR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90F952-1659mCherry1,0601,0801,1001,1201,1401,1601,1801,2001,220
    CCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCGGCTCCCGAAGTTCACCCTCGCGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGAloIBplIPstISbfIBstEIIP92EG94FK96WE98RV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150952-1659mCherry1,2401,2601,2801,3001,3201,3401,3601,3801,400
    CTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACGAGGCTCGCCTACATGGGGCTCCTGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGBplIBsgIKasIKasINarINarISfoISfoIBbvCIPluTIPluTIBpu10ISE152RM154YP156ED158GA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208952-1659mCherry1,4201,4401,4601,4801,5001,5201,5401,560
    AACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGTTGCTCCTGATGTGGTAGCACCTTGTCATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCAleIMluIXcmIBsrGISgrAID1A2L3E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V271669-1767NE210DY212TI214VE216QY218ER220AE222GR224HS226TG228GM230DE232LY234KS236952-1659mCherry5' StopGo (2A; Loughran 2017)homolog1,5801,6001,6201,6401,6601,6801,7001,7201,740
    AGTCCAACCCCGGTCCCggtaccggatccTCCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATTCAGGTTGGGGCCAGGGccatggcctaggAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAAgeIBaeIKpnISpeIBamHIAcc65IT1G2E1A2R3H4K5Q6K7I81903-2001S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T371780-1890T1S2E28S29N30P31G32P331669-1767G1S2G1T2ADAR substrate3xMS2 SGR5' stem3' StopG...n 2017)3' stem5' St...017)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    CGTAGCCCCAGTAAAGCAAACACTCAACTTCGATCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctgacgacaaggaaactcgctcaaGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAagatccgacctgttctcgtttcagtatttgcctcgagaccttaatgagttaccacagccatagcttccggactgctgttcctttgagcgagttPfoITliIXhoIPaqCIPspXIPaeR7IV9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331903-2001SR2LD4KS6KV8IN10GA12LE14LL16NG18VG20IE22GL24TT26RK28LA30Q2008-3093S1S2rTetR3' StopGo (2A; Loughran 2017)homolog1,9401,9601,9802,0002,0202,0402,0602,0802,100
    aagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggcTctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcataccgctgtgctctccttcgaccctcaactcgtcggatgggacatgaccgtgcacttcttgttcgcccgAgacgagctacgggacggttagctctacgacctgtccgtagtatgggtgaagacgggggaccttccgctcagtaccgttctgaaagacgccttgttgcggttcagtatggcgacacgagaggPmlIK32LG34VE36QP38TL40YW42HV44KN46KR48AL50LD52AL54PI56EM58LD60RH62HT64HF66CP68LE70GE72SW74QD76FL78RN80NA82KS84YR86CA88L2008-3093rTetR2,1202,1402,1602,1802,2002,2202,2402,260
    tctcacatcgcgacggggctaaagtgcatctcggcacccgcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaagagtgtagcgctgccccgatttcacgtagagccgtgggcgggttgtctctttgtcatgctttgggaccttttagtcgagcgcaaggacacagtcgttccgaagagggacctcttgcgtgacatgcgagacaggcggcacccggtgaaatgtgacccgacgcataacctccttgtNruIPsrIL90SH92RD94GA96KV98HL100GT102RP104TE106KQ108YE110TL112EN114QL116AF118LC120QQ122GF124SL126EN128AL130YA132LS134AV136GH138FT140LG142CV144LE146EQ1482008-3093rTetR2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ggagcatcaagtagcaaaagaggaaagagagacacctaccaccgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatTtgtggcctggagaaacagctaaagtgcgaaagcggcgggcctcgtagttcatcgttttctcctttctctctgtggatggtggctaagatacgggggtgaagactctgttcgttaactcgacaagctggccgtccctcggcttggacggaaggaaaagccggaccttgattagtaAacaccggacctctttgtcgatttcacgctttcgccgcccAloIMfeIEH150QV152AK154EE156RE158TP160TT162DS164MP166PL168LR170QA172IE174LF176DR178QG180AE182PA184FL186FG188LE190LI192IC194GL196EK198QL200KC202ES204GG2062008-3093rTetR2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccgaccGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGggctggCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCBlpIBsgIBstXIPT208EG210QS212DE214RA216LL218DQ220LH222TL224LS226NT228DA230TG232LE234EI236DR238AL240GI242PE244LV246NQ248GQ250AL252EP254KQ256DG258GW260GS262MA2642008-3093NZF DomainN (NCOA3) Activation Domainhomolog2,6402,6602,6802,7002,7202,7402,7602,7802,800
    AGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAABmgBIEcoNIEcoRIEE266FV268TL270KD272VG274MD276FT278LG280DW282EQ284LG286LE288QG290DT292FW294DT296AL298DN300CQ302DL304FL306LD308PP310GG312GD314GL316GL318GH320EK322F2008-3093NZF DomainZ (ZNF473) Activation DomainF (F...ain2,8202,8402,8602,8802,9002,9202,9402,960
    CCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCGGATCTcccaagaaaaaacgcaaggtgtagCTCGAGCTGGTACTGCATGCACGCAATGCTAGCGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGCCTAGAgggttcttttttgcgttccacatcGAGCTCGACCATGACGTACGTGCGTTACGATCGBmtINheIPciISphITliIXhoIBstXIPspXIPaeR7IP324SD326LD328LD330MF332NG334SL336EC338DM340ES342II344RS346EL348MD350AD352GL354DF356NF358DS360GS3622008-3093P1K2K3K4R5K6V73094-3114*1NZF DomainF (FOXO3) Activation Domainhybrid UTR3SV40_NLShomolog2,9803,0003,0203,0403,0603,0803,1003,1203,140
    TGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATACGGGGAAAGGGCAGGACCCATGGGGCTCAGAGGGGGCTGGAGCCCAGGGTCCATACGAGGGTGGAGGTGGACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTABlpIKflIKpnIPaqCIAcc65Ihybrid UTR33,1603,1803,2003,2203,2403,2603,2803,3003,320
    TAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatATTGGAAATCGTTATTTGCTTTCAAATTGATTCGATATGATTGGGGTCCCAACCAGTTAAAGCACGGTCGGTGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactaApaIBclIBmtINheIPmeIXcmIPspOMIhybrid UTR3c-myc epitopePolyhi...e taghomolog3,3403,3603,3803,4003,4203,4403,4603,4803,500
    cagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacaggtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcBGH polyadenylation sequence3,5203,5403,5603,5803,6003,6203,6403,660
    caagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcggttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcClaISphIBspDIBGH polyadenylation sequenceBGH pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggtttttKflINaeINgoMIV3,8603,8803,9003,9203,9403,9603,9804,0004,020
    acttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggctgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgBarIMscIXbaIPflFIPshAITth111IHygromycinFRT4,0404,0604,0804,1004,1204,1404,1604,1804,200
    gaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataaEcoRIHygromycin4,2204,2404,2604,2804,3004,3204,3404,360
    gcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgcgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcPstIPvuIAsiSIRsrIIDraIIIHygromycin4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacgactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcNdeIPflFIDraIIITth111IHygromycin4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcaAjuISacIIHygromycin4,7404,7604,7804,8004,8204,8404,8604,8804,900
    atatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatctHygromycin4,9204,9404,9604,9805,0005,0205,0405,060
    agtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttctcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagNaeIPfoIScaINgoMIVHygromycin5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcaBsmIPsiIBstZ17ISV40 pA5,2605,2805,3005,3205,3405,3605,3805,4005,420
    aatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctttagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcga5,4405,4605,4805,5005,5205,5405,5605,5805,600
    gcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaaccgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgPciISapIBspQI5,6205,6405,6605,6805,7005,7205,7405,760
    atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcpUC ori5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaapUC ori5,9605,9806,0006,0206,0406,0606,0806,1006,120
    atccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgtaggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacpUC ori6,1406,1606,1806,2006,2206,2406,2606,2806,300
    ctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgapUC ori6,3206,3406,3606,3806,4006,4206,4406,460
    cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcgtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtagBspHIAmp (bla)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    catagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgccgtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggBsaIAmp (bla)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    tccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgtaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaAclIFspIAmp (bla)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacBcgIPvuIScaIAmp (bla)7,0207,0407,0607,0807,1007,1207,1407,160
    tatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatctatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaAclIXmnIAmp (bla)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    tcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttaSspIBspHIAmp (bla)bla promoter7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctcataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgaSgrDIbla promoter7,5407,5607,5807,6007,6207,6407,6607,6807,700
    gagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcctcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWIBpu10I7,7207,7407,7607,7807,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7816 bp55006000650070007500500100015003' stemmutation siteopposite edited A1903-2001EKo438.4 (DNA)XZp390.1 (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)CMV,SpeI,NdeICMV promoter (with TetOs),A,CMV-Forward Genewiz (DNA),SnaBI,CspCITATA box,TetO,TetR binding sites,TetO,CMVF1 (DNA),ToFlpF (DNA),+3homolog,HindIII residue,T7 insert,5' IVT primer,XZp072.1 (DNA),+5TEV leader UTR5,homolog,kozak,949-951,XZp072.2 (DNA),+8Che_8 (DNA)EKo230.1 (DNA),mChe_4 (DNA),mChe_1 (DNA),BstEIImChe_3 (DNA),pNSK093 (DNA),BstEIImCherry,952-1659,Che_6 (DNA),PstI,SbfI,AloIBplI,BplI,KasI,NarI,SfoI,PluTINK-cherry-seq (DNA),NK-cherry-seq (DNA),BbvCI,Bpu10I,+5mChe_2 (DNA),Che_7 (DNA),AleI,SgrAIhomolog,1660-1668,XZp073.1 (DNA),pNSK124 (DNA),+65' StopGo (2A; Loughran 2017),5' stem,edited,+7ADAR substrate,bulged A -- edited?,bulged,+12bulge opposite the other A,bshti site,+7pNSK126 (DNA),EKo387.3 (DNA)pUCoriR (DNA)pUC oriBspHIEKo152.2 (DNA)BsaIFspI,AclIAmp (bla),PvuIScaI,BcgIAclI,XmnIbla promoter,FlpInF1 (DNA),FlpInF (DNA),EKo152.4 (DNA),SspI,BspHI,SgrDI,MfeI,+3

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