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XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttaSpeICMVCMV promoter (with TetOs)20406080100120140160
    agggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagNdeICMVCMV promoter (with TetOs)180200220240260280300320340
    tacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccatatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaCspCISnaBICMVCMV promoter (with TetOs)360380400420440460480500520
    tgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctBsaBIBsaBISgrDICMV promoter (with TetOs)CMVTetR binding sitesTetOTetO540560580600620640660680700
    ccgatccagcctccggactctagcgtttaaacttaagctcgctagagatccgcggccgctaatacgactcactatagggagaaaaataacaaatctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttaaatcatttcttttaggctaggtcggaggcctgagatcgcaaatttgaattcgagcgatctctaggcgccggcgattatgctgagtgatatccctctttttattgtttagagttgtgttgtatatgttttgtttgcttagagttcgttagttcgtaagatgaagataacgtcgttaaatttagtaaagaaaatBsmINotIPmeISwaIAflIISacIITEV leader UTR55' IVT primerT7 inserthomologCMV ...Os)720740760780800820840860880
    aagcaaaagcaattttctgaaaattttcaccatttacgaacgataAAGCTTcgccaccATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCttcgttttcgttaaaagacttttaaaagtggtaaatgcttgctatTTCGAAgcggtggTACCACTCGTTCCCGCTCCTCCTATTGTACCGGTAGTAGTTCCTCAAGTACGCGAAGTTCCACGTGTACCTCCCGAGGCACTTGCCGGTGCTCAAGCTCTAGCTCCCGCTCCCGCTCCCGAleIMscIXmnIHindIIIM1VS2KG4EE6DN8MA10II12KE14FM16RF18KV20HM22EG24SV26NG28HE30FE32IE34GE36GE38G952-1659mCherryTEV leader UTR5homolog9009209409609801,0001,0201,0401,060
    CGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATTCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGGGGATGCTCCCGTGGGTCTGGCGGTTCGACTTCCACTGGTTCCCACCGGGGGACGGGAAGCGGACCCTGTAGGACAGGGGAGTCAAGTACATGCCGAGGTTCCGGATGCACTTCGTGGGGCGGCTGTAAGGGCTGATGAACTTCGACAGGAAGGGGCTCCCGAAGTTCACCCTCGBstEIIR40PY42EG44TQ46TA48KL50KV52TK54GG56PL58PF60AW62DI64LS66PQ68FM70YG72SK74AY76VK78HP80AD82IP84DY86LK88LS90FP92EG94FK96WE98952-1659mCherry1,0801,1001,1201,1401,1601,1801,2001,2201,240
    GCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGCACTACTTGAAGCTCCTGCCGCCGCACCACTGGCACTGGGTCCTGAGGAGGGACGTCCTGCCGCTCAAGTAGATGTTCCACTTCGACGCGCCGTGGTTGAAGGGGAGGCTGCCGGGGCATTACGTCTTCTTCTGGTACCCGACCCTCCGGAGGAGGCTCGCCTACATGGGGCTCCTAloIBplIBplIPstISbfIBstEIIRV100MN102FE104DG106GV108VT110VT112QD114SS116LQ118DG120EF122IY124KV126KL128RG130TN132FP134SD136GP138VM140QK142KT144MG146WE148AS150SE152RM154YP156ED158952-1659mCherry1,2601,2801,3001,3201,3401,3601,3801,4001,420
    CGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGGCCGCGGGACTTCCCGCTCTAGTTCGTCTCCGACTTCGACTTCCTGCCGCCGGTGATGCTGCGACTCCAGTTCTGGTGGATGTTCCGGTTCTTCGGGCACGTCGACGGGCCGCGGATGTTGCAGTTGTAGTTCAACCTGTAGTGGAGGGTGTTGCTCCTGATGTGGTAGCACCTTGTCAleIBsgIKasIKasINarINarISfoISfoIBbvCIPluTIPluTIBpu10IGA160LK162GE164IK166QR168LK170LK172DG174GH176YD178AE180VK182TT184YK186AK188KP190VQ192LP194GA196YN198VN200IK202LD204IT206SH208NE210DY212TI214VE216Q952-1659mCherry1,4401,4601,4801,5001,5201,5401,5601,5801,600
    TACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGAGCgacgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGGGAGACGTCGAGTCCAACCCCGGTCCCggtaccggatccTATGCTTGCGCGGCTCCCGGCGGTGAGGTGGCCGCCGTACCTGCTCGACATGTTCTCGctgcgcaacCTTCGGTCTGTGTTTGTCTTTTAACACCGAGGCCACTTTGTCTGAAACTTAAAACTGGAAGACTTCGACCGCCCTCTGCAGCTCAGGTTGGGGCCAGGGccatggcctaggAKpnIMluIXcmIBamHIBsrGISgrAIAcc65IE1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D19L20L21K22L23A24G25D26V27E28S29N30P31G32P331669-1767G1T2G1S2Y218ER220AE222GR224HS226TG228GM230DE232LY234KS236952-1659D1A2L35' StopGo (2A; Loughran 2017)mCherryhomolog1,6201,6401,6601,6801,7001,7201,7401,7601,780
    CCGTTTAGGTGGGTGGAATAGTAACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATAGTATCCCACCTACCCAGACGaccggtactagtGAGGCACGGCATAAGCAAAAGATCGTAGCCCCAGTAAAGCAAACACTCAACTTCGAGGCAAATCCACCCACCTTATCATTGCATGTGGTAGTCCCATGCAGAGTCTGTGGTAGTCCCAGACAGACCATGTCGTAGTCGCATGGTATCATAGGGTGGATGGGTCTGCtggccatgatcaCTCCGTGCCGTATTCGTTTTCTAGCATCGGGGTCATTTCGTTTGTGAGTTGAAGCTAgeIBaeISpeIT1G2E1A2R3H4K5Q6K7I8V9A10P11V12K13Q14T15L16N17F18D191903-2001T1S2S1V2*3V4G5G6I7V8T9Y10T11I12R13V14R15L16R17H18H19Q20G21L22S23G24T25A26S27A28Y29H30S31I32P33P34T35Q36T371780-1890ADAR substrate3xMS2 SGR3' StopGo (2A; Loughran 2017)5' stem3' stem1,8001,8201,8401,8601,8801,9001,9201,940
    TCTACTCAAACTCGCAGGTGATGTGGAATCTAATCCAGGACCCTCGAGTtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacAGATGAGTTTGAGCGTCCACTACACCTTAGATTAGGTCCTGGGAGCTCAagatccgacctgttctcgtttcagtatttgcctcgagaccttaatgagttaccacagccatagcttccggactgctgttcctttgagcgagttttcgaccctcaactcgtcggatgggacatgaccgtgPfoITliIXhoIPaqCIPspXIPaeR7ISR2LD4KS6KV8IN10GA12LE14LL16NG18VG20IE22GL24TT26RK28LA30QK32LG34VE36QP38TL40YW42H2008-3093L20L21K22L23A24G25D26V27E28S29N30P31G32P331903-2001S1S2rTetR3' StopGo (2A; Loughran 2017)homolog1,9601,9802,0002,0202,0402,0602,0802,1002,120
    gtgaagaacaagcgggcTctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcaccccacttcttgttcgcccgAgacgagctacgggacggttagctctacgacctgtccgtagtatgggtgaagacgggggaccttccgctcagtaccgttctgaaagacgccttgttgcggttcagtatggcgacacgagaggagagtgtagcgctgccccgatttcacgtagagccgtgggNruIPmlIV44KN46KR48AL50LD52AL54PI56EM58LD60RH62HT64HF66CP68LE70GE72SW74QD76FL78RN80NA82KS84YR86CA88LL90SH92RD94GA96KV98HL100GT1022008-3093rTetR2,1402,1602,1802,2002,2202,2402,2602,2802,300
    gcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgggttgtctctttgtcatgctttgggaccttttagtcgagcgcaaggacacagtcgttccgaagagggacctcttgcgtgacatgcgagacaggcggcacccggtgaaatgtgacccgacgcataacctccttgtcctcgtagttcatcgttttctcctttctctctgtggatggtgPsrIRP104TE106KQ108YE110TL112EN114QL116AF118LC120QQ122GF124SL126EN128AL130YA132LS134AV136GH138FT140LG142CV144LE146EQ148EH150QV152AK154EE156RE158TP160TT1622008-3093rTetR2,3202,3402,3602,3802,4002,4202,4402,4602,480
    cgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatTtgtggcctggagaaacagctaaagtgcgaaagcggcgggccgaccGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGgctaagatacgggggtgaagactctgttcgttaactcgacaagctggccgtccctcggcttggacggaaggaaaagccggaccttgattagtaAacaccggacctctttgtcgatttcacgctttcgccgcccggctggCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACAloIBsgIMfeIDS164MP166PL168LR170QA172IE174LF176DR178QG180AE182PA184FL186FG188LE190LI192IC194GL196EK198QL200KC202ES204GG206PT208EG210QS212DE214RA216LL218DQ220L2008-3093rTetRN (NCOA3) Activation DomainNZF Domainhomolog2,5002,5202,5402,5602,5802,6002,6202,6402,660
    CACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCTAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCBlpIBmgBIBstXIEcoRIH222TL224LS226NT228DA230TG232LE234EI236DR238AL240GI242PE244LV246NQ248GQ250AL252EP254KQ256DG258GW260GS262MA264EE266FV268TL270KD272VG274MD276FT278LG2802008-3093NZF DomainN (NCOA3) Activation DomainZ (ZNF473) Activation Domain2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGACACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTAPciIBstXIEcoNIDW282EQ284LG286LE288QG290DT292FW294DT296AL298DN300CQ302DL304FL306LD308PP310GG312GD314GL316GL318GH320EK322FP324SD326LD328LD330MF332NG334SL336EC338DM3402008-3093NZF DomainZ (ZNF473) Activation DomainF (FOXO3) Activation Domain2,8602,8802,9002,9202,9402,9602,9803,0003,020
    GGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCGGATCTcccaagaaaaaacgcaaggtgtagCTCGAGCTGGTACTGCATGCACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTCCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGCCTAGAgggttcttttttgcgttccacatcGAGCTCGACCATGACGTACGTGCGTTACGATCGACGGGGAAAGGGCAGGACCCATGGGGCTCAGAGGGGGCTGGAGCCCAGGGTCCABmtIKflIKpnINheISphITliIXhoIPspXIAcc65IPaeR7IES342II344RS346EL348MD350AD352GL354DF356NF358DS360GS3622008-3093P1K2K3K4R5K6V73094-3114*1hybrid UTR3NZF DomainF (FOXO3) Activation DomainSV40_NLShomolog3,0403,0603,0803,1003,1203,1403,1603,1803,200
    ATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCATACGAGGGTGGAGGTGGACGGGGTGAGTGGTGGAGACGATCAAGGTCTGTGGAGGGTTCGTGCGTCGTTACGTCGAGTTTTGCGAATCGGATCGGTGTGGGGGTGCCCTTTGTCGTCACTAATTGGAAATCGTTATTTGCTTTCAAATTGATTCGATATGATTGGGGTCCCAACCAGTBlpIPaqCIhybrid UTR33,2203,2403,2603,2803,3003,3203,3403,3603,380
    ATTTCGTGCCAGCCACACCCTGGAGCTAGCgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttTAAAGCACGGTCGGTGTGGGACCTCGATCGcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaApaIBclIBmtINheIPmeIXcmIPspOMIBGH polyadenylation sequencec-myc epitopehybrid UTR3Polyhi...e taghomolog3,4003,4203,4403,4603,4803,5003,5203,5403,560
    ccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctaSphIBGH polyadenylation sequence3,5803,6003,6203,6403,6603,6803,7003,720
    ctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctgatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaClaIBspDIBGH pA3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcataKflINaeIXbaINgoMIVFRT3,9203,9403,9603,9804,0004,0204,0404,0604,080
    aggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgattccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaBarIMscIPflFIPshAITth111IHygromycinFRT4,1004,1204,1404,1604,1804,2004,2204,2404,260
    ggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccPstIEcoRIDraIIIHygromycin4,2804,3004,3204,3404,3604,3804,4004,4204,440
    tcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcaNdeIPvuIAsiSIPflFIRsrIITth111IHygromycin4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcgcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagDraIIIHygromycin4,6404,6604,6804,7004,7204,7404,7604,7804,800
    ttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgataagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctaAjuISacIIHygromycin4,8204,8404,8604,8804,9004,9204,9404,9604,980
    gcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcgScaIHygromycin5,0005,0205,0405,0605,0805,1005,1205,1405,160
    cgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcagcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtNaeIPfoIPsiINgoMIVSV40 pA5,1805,2005,2205,2405,2605,2805,3005,3205,340
    ctgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggacccBsmIBstZ17ISV40 pA5,3605,3805,4005,4205,4405,4605,4805,500
    gtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcgcacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagcSapIBspQI5,5205,5405,5605,5805,6005,6205,6405,6605,680
    gctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttaPciIpUC ori5,7005,7205,7405,7605,7805,8005,8205,8405,860
    cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtpUC ori5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgccaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgpUC ori6,0606,0806,1006,1206,1406,1606,1806,2006,220
    tacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctpUC ori6,2406,2606,2806,3006,3206,3406,3606,3806,400
    aaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccaBspHIpUC ori6,4206,4406,4606,4806,5006,5206,5406,5606,580
    ctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcgactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagBsaIAmp (bla)6,6006,6206,6406,6606,6806,7006,7206,7406,760
    agcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggttcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccaAclIFspIAmp (bla)6,7806,8006,8206,8406,8606,8806,9006,9206,940
    atggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcPvuIAmp (bla)6,9606,9807,0007,0207,0407,0607,0807,1007,120
    taagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)7,1407,1607,1807,2007,2207,2407,2607,280
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataSspIAmp (bla)bla promoter7,3007,3207,3407,3607,3807,4007,4207,4407,460
    tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgBspHISgrDIbla promoter7,4807,5007,5207,5407,5607,5807,6007,6207,640
    atagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgctatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWIBpu10I7,6607,6807,7007,7207,7407,7607,7807,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7816 bp55006000650070007500500100015003' stemmutation siteopposite edited A1903-2001EKo438.4 (DNA)XZp390.1 (DNA)pNK018 (DNA)M13-48REV Genewiz (DNA)CMV,SpeI,NdeICMV promoter (with TetOs),A,CMV-Forward Genewiz (DNA),SnaBI,CspCITATA box,TetO,TetR binding sites,TetO,CMVF1 (DNA),ToFlpF (DNA),BsaBI,+2homolog,HindIII residue,T7 insert,5' IVT primer,XZp072.1 (DNA),+5TEV leader UTR5,homolog,kozak,949-951,XZp072.2 (DNA),+8Che_8 (DNA)EKo230.1 (DNA),mChe_4 (DNA),mChe_1 (DNA),BstEIImChe_3 (DNA),pNSK093 (DNA),BstEIImCherry,952-1659,Che_6 (DNA),PstI,SbfI,AloIBplI,BplI,KasI,NarI,SfoI,PluTINK-cherry-seq (DNA),NK-cherry-seq (DNA),BbvCI,Bpu10I,+5mChe_2 (DNA),Che_7 (DNA),AleI,SgrAIhomolog,1660-1668,XZp073.1 (DNA),pNSK124 (DNA),+65' StopGo (2A; Loughran 2017),5' stem,edited,+7ADAR substrate,bulged A -- edited?,bulged,3xMS2 SGR,+11bulge opposite the other A,bshti site,+7pNSK126 (DNA),EKo387.3 (DNA)pUCoriR (DNA)pUC oriBspHIEKo152.2 (DNA)BsaIFspI,AclIAmp (bla),PvuIScaI,BcgIAclI,XmnIbla promoter,FlpInF1 (DNA),FlpInF (DNA),EKo152.4 (DNA),SspI,BspHI,SgrDI,MfeI,+3

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