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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    cctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacataNcoINdeIPvuIAsiSIRsrIIBstAPIHygromycin20406080100120140160
    cactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcggtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgcBstAPIDraIIINmeAIIIHygromycin180200220240260280300320340
    aggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagtccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcAjuISacIIHygromycin360380400420440460480500520
    cttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgaScaIHygromycin540560580600620640660680700
    cgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattacNaeIPsiINgoMIVSV40 pAHygromycin720740760780800820840860
    gttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttacaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaatBsmIBstZ17ISV40 pA8809009209409609801,0001,0201,040
    tccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtaggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgcca1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatPciISapIBspQIpUC ori1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatattttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatpUC ori1,4201,4401,4601,4801,5001,5201,5401,560
    cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagaggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctpUC ori1,5801,6001,6201,6401,6601,6801,7001,7201,740
    cacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgapUC ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcpUC ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgcAhdIAmp (bla)2,1202,1402,1602,1802,2002,2202,2402,260
    atacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcNmeAIIIAmp (bla)2,2802,3002,3202,3402,3602,3802,4002,4202,440
    taagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctaAclIFspIAmp (bla)2,4602,4802,5002,5202,5402,5602,5802,6002,620
    cgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatPvuIScaIAmp (bla)2,6402,6602,6802,7002,7202,7402,7602,7802,800
    cgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgaggccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcAclIXmnIAmp (bla)2,8202,8402,8602,8802,9002,9202,9402,960
    caaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgSspIbla promoterAmp (bla)2,9803,0003,0203,0403,0603,0803,1003,1203,140
    atttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggctaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgSgrDI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    aaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccMfeIMluISpeIBsiWICMVCMV promoter (with TetOs)3,3403,3603,3803,4003,4203,4403,4603,4803,500
    taaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgcccccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggNdeICMVCMV promoter (with TetOs)3,5203,5403,5603,5803,6003,6203,6403,660
    tattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggtNcoICspCISnaBICMVCMV promoter (with TetOs)3,6803,7003,7203,7403,7603,7803,8003,8203,840
    agtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgtcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcacSacIBsaBIEco53kICMVCMV promoter (with TetOs)TetR binding sitesTetOTetO3,8603,8803,9003,9203,9403,9603,9804,0004,020
    atagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgaagacgatcatcgccctgagctacatcttctgcctatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacttctgctagtagcgggactcgatgtagaagacggPmeISacIAflIIBsaBIBsmBIEsp3ISgrDIEco53kIHindIIIMK2TI4IA6LS8YI10FC124164-6788CMV promoter (with TetOs)Signal/Flaghomolog4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tggtattcgccgactacaaggacgatgatgacgccagcGGCgggcaacccgggaacggcagcgccttcttgctggcacccaatagaagccatgcgccggaccacgacgtcacgcagcaaagggacgaggtgtgggtggtgggcatgggcatcgtcatgtctctcatcgtcctggcaccataagcggctgatgttcctgctactactgcggtcgCCGcccgttgggcccttgccgtcgcggaagaacgaccgtgggttatcttcggtacgcggcctggtgctgcagtgcgtcgtttccctgctccacacccaccacccgtacccgtagcagtacagagagtagcaggaccgSmaIXmaITspMILV14FA16DY18KD20DD22DA24SG26GQ28PG30NG32SA34FL36LA38PN40RS42HA44PD46HD48VT50QQ52RD54EV56WV58VG60MG62IV64MS66LI68VL70A4164-6788hB2ARSignal/Flaghomolog4,2204,2404,2604,2804,3004,3204,3404,360
    catcgtgtttggcaatgtgctggtcatcacagccattgccaagttcgagcgtctgcagacggtcaccaactacttcatcacttcactggcctgtgctgatctggtcatgggcctggcagtggtgccctttggggccgcccatattcttatgaaaatgtggacttttggcaacttcgtagcacaaaccgttacacgaccagtagtgtcggtaacggttcaagctcgcagacgtctgccagtggttgatgaagtagtgaagtgaccggacacgactagaccagtacccggaccgtcaccacgggaaaccccggcgggtataagaatacttttacacctgaaaaccgttgaagBstEIII72VF74GN76VL78VI80TA82IA84KF86ER88LQ90TV92TN94YF96IT98SL100AC102AD104LV106MG108LA110VV112PF114GA116AH118IL120MK122MW124TF126GN128F4164-6788hB2AR4,3804,4004,4204,4404,4604,4804,5004,5204,540
    tggtgcgagttttggacttccattgatgtgctgtgcgtcacggccagcattgagaccctgtgcgtgatcgcagtggatcgctactttgccattacttcacctttcaagtaccagagcctgctgaccaagaataaggcccgggtgatcattctgatggtgtggattgtgtcaggccaccacgctcaaaacctgaaggtaactacacgacacgcagtgccggtcgtaactctgggacacgcactagcgtcacctagcgatgaaacggtaatgaagtggaaagttcatggtctcggacgactggttcttattccgggcccactagtaagactaccacacctaacacagtccggSmaIStuIXmaITspMIW130CE132FW134TS136ID138VL140CV142TA144SI146ET148LC150VI152AV154DR156YF158AI160TS162PF164KY166QS168LL170TK172NK174AR176VI178IL180MV182WI184VS186G4164-6788hB2AR4,5604,5804,6004,6204,6404,6604,6804,7004,720
    ttacctccttcttgcccattcagatgcactggtatcgggccacccaccaggaagccatcaactgctatgccaatgagacctgctgtgacttcttcacgaaccaagcctatgccattgcctcttccatcgtgtccttctacgttcccctggtgatcatggtcttcgtctactccagaatggaggaagaacgggtaagtctacgtgaccatagcccggtgggtggtccttcggtagttgacgatacggttactctggacgacactgaagaagtgcttggttcggatacggtaacggagaaggtagcacaggaagatgcaaggggaccactagtaccagaagcagatgaggtcL188TS190FL192PI194QM196HW198YR200AT202HQ204EA206IN208CY210AN212ET214CC216DF218FT220NQ222AY224AI226AS228SI230VS232FY234VP236LV238IM240VF242VY244SR2464164-6788hB2AR4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ggtctttcaggaggccaaaaggcagctccagaagattgacaaatctgagggccgcttccatgtccagaaccttagccaggtggagcaggatgggcggacggggcatggactccgcagatcttccaagttctgcttgaaggagcacaaagccctcaagacgttaggcatcatcatgccagaaagtcctccggttttccgtcgaggtcttctaactgtttagactcccggcgaaggtacaggtcttggaatcggtccacctcgtcctacccgcctgccccgtacctgaggcgtctagaaggttcaagacgaacttcctcgtgtttcgggagttctgcaatccgtagtagtacEcoNIVF248QE250AK252RQ254LQ256KI258DK260SE262GR264FH266VQ268NL270SQ272VE274QD276GR278TG280HG282LR284RS286SK288FC290LK292EH294KA296LK298TL300GI302IM3044164-6788hB2AR4,9204,9404,9604,9805,0005,0205,0405,060
    ggcactttcaccctctgctggctgcccttcttcatcgttaacattgtgcatgtgatccaggataacctcatccgtaaggaagtttacatcctcctaaattggataggctatgtcaattctggtttcaatccccttatctactgccggagcccagatttcaggattgccttccaggccgtgaaagtgggagacgaccgacgggaagaagtagcaattgtaacacgtacactaggtcctattggagtaggcattccttcaaatgtaggaggatttaacctatccgatacagttaagaccaaagttaggggaatagatgacggcctcgggtctaaagtcctaacggaaggtccHpaIGT306FT308LC310WL312PF314FI316VN318IV320HV322IQ324DN326LI328RK330EV332YI334LL336NW338IG340YV342NS344GF346NP348LI350YC352RS354PD356FR358IA360FQ3624164-6788hB2AR5,0805,1005,1205,1405,1605,1805,2005,2205,240
    agcttctgtgcctgcgcaggtcttctttgaaggcctatgggaatggctactccagcaacggcaacacaggggagcagagtggatatcacgtggaacaggagaaagaaaataaactgctgtgtgaagacctcccaggcacggaagactttgtgggccatcaaggtactgtgcctagtcgaagacacggacgcgtccagaagaaacttccggatacccttaccgatgaggtcgttgccgttgtgtcccctcgtctcacctatagtgcaccttgtcctctttcttttatttgacgacacacttctggagggtccgtgccttctgaaacacccggtagttccatgacacggatcFspIPmlIStuIEcoRVEL364LC366LR368RS370SL372KA374YG376NG378YS380SN382GN384TG386EQ388SG390YH392VE394QE396KE398NK400LL402CE404DL406PG408TE410DF412VG414HQ416GT418VP420S4164-6788hB2AR5,2605,2805,3005,3205,3405,3605,3805,4005,420
    cgataacattgattcacaagggaggaattgtagtacaaatgactcactgctgGCCggtggacgcaccccacccagcctgggtccccaagatgagtcctgcaccaccgccagctcctccctggccaaggacacttcatcgGGTACCGGTGCTTTGCACTTGGATCAAACGCCATCTgctattgtaactaagtgttccctccttaacatcatgtttactgagtgacgacCGGccacctgcgtggggtgggtcggacccaggggttctactcaggacgtggtggcggtcgaggagggaccggttcctgtgaagtagcCCATGGCCACGAAACGTGAACCTAGTTTGCGGTAGAAgeIBsgIKflIKpnIXcmIEcoNIAcc65ID422NI424DS426QG428RN430CS432TN434DS436LL438AG440GR442TP444PS446LG448PQ450DE452SC454TT456AS458SS460LA462KD464TS466SG468TG470AL472HL474DQ476TP478S4164-6788V2 tailhB2ARhADAR2 cat domainhomologhomolog5,4405,4605,4805,5005,5205,5405,5605,5805,600
    CGCCAGCCTATTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCCTGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAAAAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGGCGGTCGGATAAGGGTCACTCCCAGAAGTCGACGTAAATGGCGTCCAAAATCGACTGCGACAGAGTGCGGACCAGGACCCATTCAAACCACTGGACTGGCTGTTGAAGAGGAGGGGAGTGCGAGCGTCTTTTCACGACCGACCTCAGCAGTACTGTTGTCCGTGTCTACAATTTCBstEIIR480QP482IP484SE486GL488QL490HL492PQ494VL496AD498AV500SR502LV504LG506KF508GD510LT512DN514FS516SP518HA520RR522KV524LA526GV528VM530TT532GT534DV536K4164-6788hADAR2 cat domain5,6205,6405,6605,6805,7005,7205,7405,760
    ATGCCAAGGTGATAAGTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCATTAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACACAACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGTACGGTTCCACTATTCACAAAGATGTCCTTGTTTTACATAATTACCACTTATGTACTCACTAGCACCGGAACGTAATTTACTGACGGTACGTCTTTATTATAGAGCCTCTAGGAACGAGTCTAAAGAAATATGTGTTGAACTCGAAATGAATTTATTGTTTCTACTAGTTTTTTCD538AK540VI542SV544ST546GT548KC550IN552GE554YM556SD558RG560LA562LN564DC566HA568EI570IS572RR574SL576LR578FL580YT582QL584EL586YL588NN590KD592DQ594KR5964164-6788hADAR2 cat domain5,7805,8005,8205,8405,8605,8805,9005,9205,940
    ATCCATCTTTCAGAAATCAGAGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTCCCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATAGACACCCAAATCGTAAAGCAAGAGGACAGCTACGGTAGGTAGAAAGTCTTTAGTCTCGCTCCCCCCAAATCCGACTTCCTCTTACAGGTCAAAGTAGACATGTAGTCGTGGAGAGGGACACCTCTACGGTCTTAGAAGAGTGGTGTACTCGGTTAGGACCTTCTTGGTCGTCTATCTGTGGGTTTAGCATTTCGTTCTCCTGTCGATGCCBsrGISI598FQ600KS602ER604GG606FR608LK610EN612VQ614FH616LY618IS620TS622PC624GD626AR628IF630SP632HE634PI636LE638EP640AD642RH644PN646RK648AR650GQ652LR6544164-6788hADAR2 cat domain5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ACCAAAATAGAGTCTGGTCAGGGGACGATTCCAGTGCGCTCCAATGCGAGCATCCAAGCGTGGGACGGGGTGCTGCAAGGGGAGCGGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGGGATCCCTGCTCAGCATTTTCGTGGAGCCCATGGTTTTATCTCAGACCAGTCCCCTGCTAAGGTCACGCGAGGTTACGCTCGTAGGTTCGCACCCTGCCCCACGACGTTCCCCTCGCCGACGAGTGGTACAGGACGTCACTGTTCTAACGTGCGACCTTGCACCACCCGTAGGTCCCTAGGGACGAGTCGTAAAAGCACCTCGGGTBlpIBamHITK656IE658SG660QG662TI664PV666RS668NA670SI672QA674WD676GV678LQ680GE682RL684LT686MS688CS690DK692IA694RW696NV698VG700IQ702GS704LL706SI708FV710EP7124164-6788hADAR2 cat domain6,1406,1606,1806,2006,2206,2406,2606,2806,300
    TTTACTTCTCGAGCATCATCCTGGGCAGCCTTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCACGGCAGCCAGGGAAGGCCCCCAACTTAAATGAAGAGCTCGTAGTAGGACCCGTCGGAAATGGTGCCCCTGGTGGAAAGGTCCCGGTACATGGTCGCCTAGAGGTTGTATCTCCTGGACGGTGGAGAGATGTGGGAGTTGTTCGGAAACGAGTCACCGTAGTCGTTACGTCTTCGTGCCGTCGGTCCCTTCCGGGGGTTGAATliIXhoIPaeR7IIY714FS716SI718IL720GS722LY724HG726DH728LS730RA732MY734QR736IS738NI740ED742LP744PL746YT748LN750KP752LL754SG756IS758NA760EA762RQ764PG766KA768PN770F4164-6788hADAR2 cat domain6,3206,3406,3606,3806,4006,4206,4406,460
    CAGTGTCAACTGGACGGTAGGCGACTCCGCTATTGAGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGCACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCTCCAAGATTACCAAACCCAACGTCACAGTTGACCTGCCATCCGCTGAGGCGATAACTCCAGTAGTTGCGGTGCTGACCCTTCCTACTCGACCCGGCGCGCAGGGCGGACACATTCGTGCGCAACATGACAGCGACCTACGCACACGTGCCGTTCCAAGGGAGGGTGAATGATGCGAGGTTCTAATGGTTTGGGTTGMluIS772VN774WT776VG778DS780AI782EV784IN786AT788TG790KD792EL794GR796AS798RL800CK802HA804LY806CR808WM810RV812HG814KV816PS818HL820LR822SK824IT826KP828N4164-6788hADAR2 cat domain6,4806,5006,5206,5406,5606,5806,6006,6206,640
    GTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGGCCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGAAGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCtgagccgctcgaggagggcccgaacaaaaactcatctcagCACATGGTACTCAGGTTCGACCGCCGTTTCCTCATGGTCCGGCGGTTCCGCGCAGACAAGTGTCGGAAGTAGTTCCGCCCCGACCCCCGGACCCACCTCTTCGGGTGGCTCGTCCTGGTCAAGAGTGAGTGCGGGactcggcgagctcctcccgggcttgtttttgagtagagtcApaITliIXhoIPspXIPaeR7IPspOMI*1V830YH832ES834KL836AA838KE840YQ842AA844KA846RL848FT850AF852IK854AG856LG858AW860VE862KP864TE866QD868QF870SL872TP874*4164-6788hADAR2 cat domainc-myc epitope6,6606,6806,7006,7206,7406,7606,7806,8006,820
    aagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggattctcctagacttatcgcggcagctggtagtagtagtagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctPmeIBGH polyadenylation sequencePolyhi...e tagc-my...ope6,8406,8606,8806,9006,9206,9406,9606,9807,000
    aattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaattaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgccttClaISphIBspDIBGH polyadenylation sequenceBGH pA7,0207,0407,0607,0807,1007,1207,1407,160
    agaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagtcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcNaeINgoMIV7,1807,2007,2207,2407,2607,2807,3007,3207,340
    ctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgagatttagcccccagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagaBarIKflIXbaIPshAIFRTHygromycin7,3607,3807,4007,4207,4407,4607,4807,5007,520
    gtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtaBsmBIEsp3IHygromycin7,5407,5607,5807,6007,6207,6407,6607,6807,700
    cggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctEcoRIDraIIIHygromycin7,7207,7407,7607,7807,800
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    7800 bp5500600065007000750050010001500pUC oriNew Stop6786-67884164-6788M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo152.2 (DNA)XZp023.1 (DNA)EKbF (DNA)EKo272.2 (DNA)XZp077.1 (DNA)EKo271.3 (DNA)EKo330.4 (DNA)pNSK053 (DNA)pNK018 (DNA)EKo326.3 (DNA)Hygromycin,BstAPI,NcoI,AsiSI,PvuI,RsrII,NdeI,NmeAIII,BstAPI,DraIIIAjuI,SacIIHyg_F (DNA)ScaISV40PA_R (DNA),NgoMIV,NaeI,PsiISV40 pA,SV40pA-R Genewiz (DNA),BsmI,BstZ17IM13R Genewiz (DNA)pUCori5F (DNA),BspQI,SapIPciIEKo542.3 (DNA)XZp018test (DNA)XZp018.1 (DNA)XZp004.3 (DNA),BsrGIEKo361.1 (DNA)hADAR2 cat domain,E488Q,T501A,XZp018.2 (DNA),+2XZp053.1 (DNA)BamHIBlpIXZp030.1 (DNA),EKo455.2 (DNA),XZp018.4 (DNA),+4MluIXZp053.2 (DNA),EKo330.5 (DNA),EKbR (DNA),PaeR7I,PspXI,+4c-myc epitope,Polyhistidine tag,cya,ToFlpR (DNA),+3BGH polyadenylation sequence,XZ41-test-R1 (DNA),BghR1 (DNA)bghF (DNA),SphI,BspDI,ClaIBGH pA,EKo293.3 (DNA),FlpInR (DNA)FRT,FrtF (DNA),EKo299.3 (DNA),NgoMIV,NaeI,KflI,XbaIEKo152.1 (DNA),BarI,PshAI,BsmBI,Esp3I,EcoRI,DraIII

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    7800

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