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pSI-218 Addgene
  • Sequence Map
    Editing disabled on read-only sequence.
    ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagaaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggatttaacattcgcaattataaaacaattttaagcgcaatttaaaaacaatttagtcgagtaaaaaattggttatccggctttagccgttttagggaatatttagttttcttatctggctct20406080100120140160
    tagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggatcccaactcacaacaaggtcaaaccttgttctcaggtgataatttcttgcacctgaggttgcagtttcccgctttttggcagatagtcccgctaccgggtgatgcacttggtagtgggattagttcaaaaaaccccagctccacggcatttcgtgatttagccttgggatttccAloIDraIII180200220240260280300320340
    gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcctcgggggctaaatctcgaactgcccctttcggccgcttgcaccgctctttccttcccttctttcgctttcctcgcccgcgatcccgcgaccgttcacatcgccagtgcgacgcgcattggtggtgtgggcggcgcgaattacgcggcgatgtcccgcgcagggtaagcggtaaglacZ_a360380400420440460480500520
    aggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgtccgacgcgttgacaacccttcccgctagccacgcccggagaagcgataatgcggtcgaccgctttccccctacacgacgttccgctaattcaacccattgcggtcccaaaagggtcagtgctgcaacattttgctgccggtcactcgcgcgcattatgctgagtgatatcccgclacZ_aM13_pUC_fwd_primerT7_promoterM13_f...imer540560580600620640660680700
    aattgggtaccgggccaattcgaacgctgacgtcatcaacccgctccaaggaatcgcgggcccgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtttaacccatggcccggttaagcttgcgactgcagtagttgggcgaggttccttagcgcccgggctcccggataaagggtactaaggaagtataaacgtatatgctatgttccgacaatctctctattaatcttaattaaactgacatttgtgtttctataatcatgttttatgcaApaIPspOMIU6 promoterhU6-F720740760780800820840860
    gacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccggagacggttgtaaatgagcacacaaaatacacatgctctgcatctttcattattaaagaacccatcaaacgtcaaaattttaatacaaaattttacctgatagtatacgaatggcattgaactttcataaagctaaagaaccgaaatatatagaacacctttcctgctttgtggcctctgccaacatttactcgtgtgttttatgtgtacgaBsmBIEsp3IU6 promoterfillerLKO.1 5'8809009209409609801,0001,0201,040
    aaaatattatattctatgacctttataaaatcaaccaaaatcttctttttaataactttagtatcaataattagaatttttatgttcctttttgcaaacttttaataaaaatgagcaaaataaaaaaacgctagttttagtaactcgcgttgttttcttcacctttaataatagcttttataatataagatactggaaatattttagttggttttagaagaaaaattattgaaatcatagttattaatcttaaaaatacaaggaaaaacgtttgaaaattatttttactcgttttatttttttgcgatcaaaatcattgagcgcaacaaaagaagtggaaattattatcgBaeIfiller1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tactccaccacttgttcctaagcggtcagctcctgcttcaatcattttttgagcatcttcaaatgttctaactccaccagctgctttaactaaagcattgtctttaacaactgacttcattagtttaacatcttcaaatgttgcacctgattttgaaaatcctgttgatgttttaatgaggtggtgaacaaggattcgccagtcgaggacgaagttagtaaaaaactcgtagaagtttacaagattgaggtggtcgacgaaattgatttcgtaacagaaattgttgactgaagtaatcaaattgtagaagtttacaacgtggactaaaacttttaggacaactacaaaatfiller1,2401,2601,2801,3001,3201,3401,3601,3801,400
    acaaattctaatccagcttcaacagctatttcacaagctttcatgatttcttcttttgttaataaacaattttccataatacatttaacaacatgtgatccagctgctttttttacagctttcatgtcttctaaaactaattcataatttttgtcttttaatgcaccaatatttatgtttaagattaggtcgaagttgtcgataaagtgttcgaaagtactaaagaagaaaacaattatttgttaaaaggtattatgtaaattgttgtacactaggtcgacgaaaaaaatgtcgaaagtacagaagattttgattaagtattaaaaacagaaaattacgtggttataaatfiller1,4201,4401,4601,4801,5001,5201,5401,560
    ataccatatcaatttctgttgcaccatctttaattgcttcagaaacttcgaatgcttttgtagctgttgtgcatgcacctagaggaaaacctacaacatttgttattcctacatttgtgccttttaataattctttacaatagcttgttcaatatgaattaacacaaactgttgctatggtatagttaaagacaacgtggtagaaattaacgaagtctttgaagcttacgaaaacatcgacaacacgtacgtggatctccttttggatgttgtaaacaataaggatgtaaacacggaaaattattaagaaatgttatcgaacaagttatacttaattgtgtttgacaacgfiller1,5801,6001,6201,6401,6601,6801,7001,7201,740
    aaaatcaaattcaattgcttcatcacataattgtttaatttcagctttcgtagcatcttgttttaataatgtgtgatctatatatttgtttagtttcattttttctcctatatattcatttttaattttaattctttaataatttcgtctactttaactttagcgttttgaacagttttagtttaagttaacgaagtagtgtattaacaaattaaagtcgaaagcatcgtagaacaaaattattacacactagatatataaacaaatcaaagtaaaaaagaggatatataagtaaaaattaaaattaagaaattattaaagcagatgaaattgaaatcgcaaaacttgtcMfeIfiller1,7601,7801,8001,8201,8401,8601,8801,9001,920
    attcaccaacacctataaaataaatttttagtttaggttcagttccacttgggcgaacagcaaatcatgacttatcttctaaataaaattttagtaagtcttgtcctggcatattatacattccatcgatgtagtcttcaacattaacaactttaagtccagcaatttgagttaataagtggttgtggatattttatttaaaaatcaaatccaagtcaaggtgaacccgcttgtcgtttagtactgaatagaagatttattttaaaatcattcagaacaggaccgtataatatgtaaggtagctacatcagaagttgtaattgttgaaattcaggtcgttaaactcaattClaIBspDIfiller1,9401,9601,9802,0002,0202,0402,0602,0802,100
    gggtgttgctctcaatgatttcattaatggttcaatttttaatttcttttcttctggtttaaaattcaagtttaaagtgaaagtgtaatatgcacccatttctttaaataaatcttctaaatagtctactaatgttttattttgttttttataaaatcaagcagcctctgctattcccacaacgagagttactaaagtaattaccaagttaaaaattaaagaaaagaagaccaaattttaagttcaaatttcactttcacattatacgtgggtaaagaaatttatttagaagatttatcagatgattacaaaataaaacaaaaaatattttagttcgtcggagacgataaBstAPIfiller2,1202,1402,1602,1802,2002,2202,2402,260
    aatatagaagcttgtattccatctttatctctagctgagtcatcaattacatatccataactttcttcataagcaaaaacaaaatttaatccgttatcttcttctttagcaatttctctacccattcatttaaatccagttaaagtttttacaatattaactccatatttttcatttatatcttcgaacataaggtagaaatagagatcgactcagtagttaatgtataggtattgaaagaagtattcgtttttgttttaaattaggcaatagaagaagaaatcgttaaagagatgggtaagtaaatttaggtcaatttcaaaaatgttataattgaggtataaaaagtaSwaIfiller2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gagcgattctatcacccaaatcacttgttacaaaacttgaatatagagccggattttttggaatgctatttaagcgttttagatttgataattttcaatcaattaaaattggtcctgtttgatttccatctaatcttacaaaatgaccatcatgttttattgccattccaaatctctcgctaagatagtgggtttagtgaacaatgttttgaacttatatctcggcctaaaaaaccttacgataaattcgcaaaatctaaactattaaaagttagttaattttaaccaggacaaactaaaggtagattagaatgttttactggtagtacaaaataacggtaaggtttagafiller2,4602,4802,5002,5202,5402,5602,5802,6002,620
    gtcagcatctgggtcattcataataataatatctgcatcatgtttaataccatattcaagcggtatttttcatgcaggatcaaattctggatttggatttacaacatttttaaatgtttcatcttcaaatgcatgctcttcaacctcaataacgttatatcctgattcacgtaatcagtcgtagacccagtaagtattattattatagacgtagtacaaattatggtataagttcgccataaaaagtacgtcctagtttaagacctaaacctaaatgttgtaaaaatttacaaagtagaagtttacgtacgagaagttggagttattgcaatataggactaagtgcattaNsiIfiller2,6402,6602,6802,7002,7202,7402,7602,7802,800
    atttttggggtaaatttagttcctgttccattaactgcgctaaaaataatttttaaatcttttttagcttcttgctcttttttgtacgtctctgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttaaaaaccccatttaaatcaaggacaaggtaattgacgcgatttttattaaaaatttagaaaaaatcgaagaacgagaaaaaacatgcagagacaaaatctcgatctttatcgttcaattttattccgatcaggcaatagttgaactttttcaccgtggctcagccacgaaaaaaPsrIBsmBIEsp3IfillergRNA scaffold2,8202,8402,8602,8802,9002,9202,9402,960
    acgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaatgcgcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattMluISpeICMV_immearly_promoterCAG_enhancer2,9803,0003,0203,0403,0603,0803,1003,1203,140
    cgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaacCMV_immearly_promoterCAG_enhancer3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgNcoISnaBICMV_immearly_promoterCAG_enhancer3,3403,3603,3803,4003,4203,4403,4603,4803,500
    aactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattaatacgactcactatagggagacccaagctggctagcgaaatggcaccgaagaagaagcgtaaattgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagaccgattgatctcttgggtgacgaatgaccgaatagctttaattatgctgagtgatatccctctgggttcgaccgatcgctttaccgtggcttcttcttcgcatttBmtINheICMV_immearly_promoterNLS UnitCMV_fwd_primerT7_promoterSV40 NLS3,5203,5403,5603,5803,6003,6203,6403,660
    gtcggaatccacggagttcctgcggcaatggacaagaagtactccattgggctcgctatcggcacaaacagcgtcggttgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgatcgccacagcataaagaagaacctcattggcgcagccttaggtgcctcaaggacgccgttacctgttcttcatgaggtaacccgagcgatagccgtgtttgtcgcagccaacccggcagtaatgcctgctcatgttccacggctcgttttttaagtttcaagacccgttatggctagcggtgtcgtatttcttcttggagtaaccgcsp_nCas9 (D10A)NLS Unit3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ccctcctgttcgactccggggaaacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttccataggctggaggagtcctttttggtggagggaggacaagctgaggcccctttgccggcttcggtgcgccgagttttcttgtcgtgccgcgtctatatgggcgtctttcttagcctagacgatggacgtcctctagaaatcattactctaccgattccacctactgagaaagaaggtatccgacctcctcaggaaaaaccacctSbfIBglIIsp_nCas9 (D10A)3,8603,8803,9003,9203,9403,9603,9804,0004,020
    ggaggataaaaagcacgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggcgcatatgatcaaatttcggggacctcctatttttcgtgctcgcggtgggttagaaaccgttatagcacctgctccaccgcatggtacttttcatgggttggtatatagtagactccttcttcgaacatctgtcatgactattccgactgaacgccaactagatagagcgcgaccgcgtatactagtttaaagcccctXcmIsp_nCas9 (D10A)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    cacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccggcggctcgaaaaccgtgaaggagtagctccccctggacttgggtctgttgtcgctacagctgtttgagaaataggttgaccaagtctgaatgttagtcgaaaagcttctcttgggctagttgcgtaggcctcaactgcggtttcgttaggactcgcgatccgacaggtttagggccgccgagcttttggSalIBspEIEcoNIsp_nCas9 (D10A)4,2204,2404,2604,2804,3004,3204,3404,360
    tcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcccagatcggagtagcgtgtcgagggacccctcttcttcttgccggacaaaccattagaatagcgggacagtgagcccgactgggggttgaaatttagattgaagctggaccggcttctacggttcgaagttgactcgtttctgtggatgctactactagagctgttagacgaccgggtctagccsp_nCas9 (D10A)4,3804,4004,4204,4404,4604,4804,5004,5204,540
    cgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgtcagacaggctggtcatgcgtctggaaaaaaaccgccgtttcttggacagtctgcggtaagacgactcactataagacgctcacttgtgcctctagtggtttcgaggcgactcgcgatcatactagttcgcgatactactcgtggtggttctgaactgaaacgacttccgggaacagtctgtcsp_nCas9 (D10A)4,5604,5804,6004,6204,6404,6604,6804,7004,720
    caactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgttgcgttgacggactcttcatgttcctttaaaagaagctagtcagatttttaccgatgcggcctatgtaactgccgcctcgttcggtcctccttaaaatgtttaaataattcgggtagaaccttttttacctgccgtggctcctcgacgaccatttcgaattgtctcttctagacaacgBglIIsp_nCas9 (D10A)4,7404,7604,7804,8004,8204,8404,8604,8804,900
    gcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggcgtttgtcgcgtgaaagctgttaccttcgtagggggtggtctaagtggacccgcttgacgtgcgataggagtccgccgttctcctaaagatggggaaaaactttctattgtcccttttctaactcttttaggagtgtaaagcctatgggatgatacatccgggggagcgggccccBsgISmaIXmaITspMIBsu36Isp_nCas9 (D10A)4,9204,9404,9604,9805,0005,0205,0405,060
    aaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtttaaggtctaagcgcacctactgagcgtttagtcttctctggtagtgagggaccttgaagctccttcagcacctattcccccggagacgggtcaggaagtagctttcctactgattgaaactatttttagacggattgcttttccacgaaggatttgtgagagacgacatgctcsp_nCas9 (D10A)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tacttcacagtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttaccgtgaaacagctcaaagaagactatttcaaaaagattgaatgttatgaagtgtcaaatattgctcgagtggttccagtttatgcagtgtcttccctactctttcggtcgtaaggacagacctctcgtcttctttcgatagcacctggaggagaagttctgcttggcctttcaatggcactttgtcgagtttcttctgataaagtttttctaacttacaaBarIsp_nCas9 (D10A)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcgactctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtcctcacccttacgttgtttgaagatagggagatgattgaagctgagacaactttagtcgcctcacctcctagcgaagttgcgtagggacccttgcatagtgctagaggacttttagtaatttctgttcctgaaggacctgttactcctcttgctcctgtaagaactcctgtaacaggagtgggaatgcaacaaacttctatccctctactaactsp_nCas9 (D10A)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    agaacgcttgaaaacttacgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatgggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaatcctggattttcttaagtccgatggatttgccaaccggtcttgcgaacttttgaatgcgagtagagaagctgctgtttcagtactttgtcgagttctccgcggctatatgtcctacccccgccgacagttcttttgactagttaccctaggctctgttcgtctcacctttctgttaggacctaaaagaattcaggctacctaaacggttggccAflIIBamHIsp_nCas9 (D10A)5,6205,6405,6605,6805,7005,7205,7405,760
    aacttcatgcagttgatccatgatgactctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacagtcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgcagaccgttaaggtcgtggatgaactcgtcaaagttgaagtacgtcaactaggtactactgagagagtggaaattcctcctgtaggtctttcgtgttcaaagaccggtccccctgtcagaagtgctcgtgtagcgattagaacgtccatcgggtcgatagtttttcccttatgacgtctggcaattccagcacctacttgagcagtttcAjuIsp_nCas9 (D10A)5,7805,8005,8205,8405,8605,8805,9005,9205,940
    taatgggaaggcataagcccgagaatatcgttatcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtagggaaaggatgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaacacccagttgaaaacacccagcttcagaaattacccttccgtattcgggctcttatagcaatagctctaccgggctctcttggtttgatgggtcttccctgtcttcttgtcatccctttcctacttctcctaacttctcccatattttcttgaccccagggtttaggaattccttgtgggtcaacttttgtgggtcgaagtcttKflIAflIIsp_nCas9 (D10A)5,9605,9806,0006,0206,0406,0606,0806,1006,120
    tgagaagctctacctgtactacctgcagaacggcagggacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtggatcatatcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaagatccgataaaaatagagggaagactcttcgagatggacatgatggacgtcttgccgtccctgtacatgcacctagtccttgacctgtagttagccgagaggctgatgctgcacctagtatagcacggggtcagaaaagagtttctactaagataactattatttcacaactgttctaggctatttttatctcccttcBmgBIsp_nCas9 (D10A)6,1406,1606,1806,2006,2206,2406,2606,2806,300
    agtgataacgtcccctcagaagaagttgtcaagaaaatgaaaaattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataatctgactaaggctgaacgaggtggcctgtctgagttggataaagccggcttcatcaaaaggcagcttgttgagacactcactattgcaggggagtcttcttcaacagttcttttactttttaataaccgccgtcgacgacttgcggtttgactagtgtgttgccttcaagctattagactgattccgacttgctccaccggacagactcaacctatttcggccgaagtagttttccgtcgaacaactctgtgsp_nCas9 (D10A)6,3206,3406,3606,3806,4006,4206,4406,460
    gccagatcaccaagcacgtggcccaaattctcgattcacgcatgaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaagtctaagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaacaattaccaccacggtctagtggttcgtgcaccgggtttaagagctaagtgcgtacttgtggttcatgctacttttactgtttgactaagctctccactttcaataatgagacttcagattcgaccagagtctaaagtctttcctgaaagtcaaaatattccactctctctagttgttaatggtggtPmlIPsrIBstXIsp_nCas9 (D10A)6,4806,5006,5206,5406,5606,5806,6006,6206,640
    tgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaaaaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttaggaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttcttttacagcaatacgcgtactacggatggacttacgtcaccatccgtgacgtgaatagttttttatagggttcgaacttagacttaaacaaatgcctctgatatttcacatgctacaatccttttactagcgtttcagactcgtcctttatccgttccggtggcgattcatgaagaaaatgtcgttaBsgIFspAIsp_nCas9 (D10A)6,6606,6806,7006,7206,7406,7606,7806,8006,820
    attatgaattttttcaagaccgagattacactggccaatggagagattcggaagcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtagggatttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccgaagtaatacttaaaaaagttctggctctaatgtgaccggttacctctctaagccttcgctggtgaatagctttgtttgcctctttgtcctctttagcacaccctgttcccatccctaaagcgctgtcaggccttccaggacaggtacggcgtccacttgtagcaatttttctggcttcNruIBspEIPaqCIsp_nCas9 (D10A)6,8406,8606,8806,9006,9206,9406,9606,9807,000
    tacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagctgatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagtcgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaatgtctggcctccgaagaggttcctttcataggagggcttttccttgtcgctgttcgactagcgtgcgttttttctaaccctggggttctttatgccgcctaagctaagaggatgtcagcgaatgtcacatgaccaacaccggtttcacctctttcccttcagattttttgagttKflIsp_nCas9 (D10A)7,0207,0407,0607,0807,1007,1207,1407,160
    aagcgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccatcgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcccaagtactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtttcgcagttccttgacgacccgtagtgttagtacctcgctagttcgaagctttttttggggtagctgaaagagctccgctttcctatatttctccagttttttctggagtagtaattcgaagggttcatgagagagaaactcgaacttttgccggcctttgcttacgagcgatcaTliIXhoIPaeR7Isp_nCas9 (D10A)7,1807,2007,2207,2407,2607,2807,3007,3207,340
    gcgggcgagctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggccagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgtggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgcgcccgctcgacgtctttccattgctcgaccgtgacgggagatttatgcaattaaagaacatagaccggtcggtgatacttttcgagtttcccagagggcttctattactcgtcttcgtcgacaagcaccttgttgtgtttgtgatggaactactctagtagctcgtttattcgcsp_nCas9 (D10A)7,3607,3807,4007,4207,4407,4607,4807,5007,520
    aattctccaaaagagtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacagggataagcccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttgggcgcgcctgcagccttcaagtacttcgacaccaccatagacagttaagaggttttctcactaggagcggctgcgattggagctattccacgaaagacgaatgttattcgtgtccctattcgggtagtccctcgtccgtcttttgtaataggtgaacaaatgagactggttgaacccgcgcggacgtcggaagttcatgaagctgtggtggtatctgtcAscIEcoNIEcoRIsp_nCas9 (D10A)7,5407,5607,5807,6007,6207,6407,6607,6807,700
    aaagcggtacacctctacaaaggaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaagaatcgacctctctcagctcggtggagacagcagggctgaccccaagaagaagaggaaggtgggtggaggaggtaccggcggtggaggctcagcagaatttcgccatgtggagatgtttcctccaggacctgcggtgtgactaagtagtcagttaatgccccgagatactttgttcttagctggagagagtcgagccacctctgtcgtcccgactggggttcttcttctccttccacccacctcctccatggccgccacctccgagtcgtcttsp_nCas9 (D10A)2x GS linkerNLS7,7207,7407,7607,7807,8007,8207,8407,860
    tacgtacgagctctgtttgacttcaatgggaatgacgaggaggatctcccctttaagaagggcgatattctccgcatcagagataagcccgaagaacaatggtggaatgccgaggatagcgaagggaaaaggggcatgattctggtgccatatgtggagaaatattccggtgactatgcatgctcgagacaaactgaagttacccttactgctcctcctagaggggaaattcttcccgctataagaggcgtagtctctattcgggcttcttgttaccaccttacggctcctatcgcttcccttttccccgtactaagaccacggtatacacctctttataaggccactgaBsiWISnaBIMutated SH3 domain3x...r7,8807,9007,9207,9407,9607,9808,0008,0208,040
    acaaagaccatgatggggattacaaagaccacgacatcgactacaaagacgacgacgataaatcagggatgacagacgccgagtacgtgcgcattcatgagaaactggatatttacaccttcaagaagcagttcttcaacaacaagaaatctgtgtcacaccgctgctacgtgcttgtttctggtactacccctaatgtttctggtgctgtagctgatgtttctgctgctgctatttagtccctactgtctgcggctcatgcacgcgtaagtactctttgacctataaatgtggaagttcttcgtcaagaagttgttgttctttagacacagtgtggcgacgatgcacgaFspAIPmCDA13x Flag plus linkerFLAGFLAGFLAG8,0608,0808,1008,1208,1408,1608,1808,2008,220
    gtttgagttgaagcgaaggggcgaaagaagggcttgcttttggggctatgccgtcaacaagccccaaagtggcaccgagagaggaatacacgctgagatattcagtatccgaaaggtggaagagtatcttcgggataatcctgggcagtttacgatcaactggtattccagctggcaaactcaacttcgcttccccgctttcttcccgaacgaaaaccccgatacggcagttgttcggggtttcaccgtggctctctccttatgtgcgactctataagtcataggctttccaccttctcatagaagccctattaggacccgtcaaatgctagttgaccataaggtcgaccPmCDA18,2408,2608,2808,3008,3208,3408,3608,3808,400
    agtccttgcgctgattgtgccgagaaaattctggaatggtataatcaggaacttcggggaaacgggcacacattgaaaatctgggcctgcaagctgtactacgagaagaatgcccggaaccagataggactctggaatctgagggacaatggtgtaggcctgaacgtgatggttttcaggaacgcgactaacacggctcttttaagaccttaccatattagtccttgaagcccctttgcccgtgtgtaacttttagacccggacgttcgacatgatgctcttcttacgggccttggtctatcctgagaccttagactccctgttaccacatccggacttgcactaccaaaStuIPmCDA18,4208,4408,4608,4808,5008,5208,5408,560
    ccgagcactatcagtgttgtcggaagattttcatccaaagctctcataaccagctcaatgaaaaccgctggttggagaaaacactgaaacgtgcggagaagtggagatccgagctgagcatcatgatccaggtcaagattctgcataccactaagtctccagccgttggtcccaaggctcgtgatagtcacaacagccttctaaaagtaggtttcgagagtattggtcgagttacttttggcgaccaacctcttttgtgactttgcacgcctcttcacctctaggctcgactcgtagtactaggtccagttctaagacgtatggtgattcagaggtcggcaaccagggttPmCDA18,5808,6008,6208,6408,6608,6808,7008,7208,740
    gaagaaaagaaaagtcggtaccatgaccaacctttccgacatcatagagaaggaaacaggcaaacagttggtcatccaagagtcgatactcatgcttcctgaagaagttgaggaggtcattgggaataagccggaaagtgacattctcgtacacactgcgtatgatgagagcacccttcttttcttttcagccatggtactggttggaaaggctgtagtatctcttcctttgtccgtttgtcaaccagtaggttctcagctatgagtacgaaggacttcttcaactcctccagtaacccttattcggcctttcactgtaagagcatgtgtgacgcatactactctcgtggUgiSV40 NLS8,7608,7808,8008,8208,8408,8608,8808,9008,920
    gatgagaacgtgatgctgctcacgtcagatgccccagagtacaaaccctgggctctggtgattcaggactctaatggagagaacaagatcaagatgctagaaggcaggggaagccttctgacttgtggggatgtggaagaaaaccctggtccatctagaatgattgaacaagatgctactcttgcactacgacgagtgcagtctacggggtctcatgtttgggacccgagaccactaagtcctgagattacctctcttgttctagttctacgatcttccgtccccttcggaagactgaacacccctacaccttcttttgggaccaggtagatcttactaacttgttctacXbaIBmgBIUgiT2A+linkerNeoR/KanR8,9408,9608,9809,0009,0209,0409,0609,0809,100
    gattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcctaacgtgcgtccaagaggccggcgaacccacctctccgataagccgatactgacccgtgttgtctgttagccgacgagactacggcggcacaaggccgacagtcgcgtccccgcgggccaagaaaaacagttctggctggacaggccacgggacttacttgacgtcctgctccgNeoR/KanR9,1209,1409,1609,1809,2009,2209,2409,260
    agcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggtcgcgccgatagcaccgaccggtgctgcccgcaaggaacgcgtcgacacgagctgcaacagtgacttcgcccttccctgaccgacgataacccgcttcacggccccgtcctagaggacagtagagtggaacgaggacggctctttcataggtagtaccgactacgttacgccgccNeoR/KanR9,2809,3009,3209,3409,3609,3809,4009,4209,440
    ctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggacgtatgcgaactaggccgatggacgggtaagctggtggttcgctttgtagcgtagctcgctcgtgcatgagcctaccttcggccagaacagctagtcctactagacctgcttctcgtagtccccgagcgcggtcggcttgacaagcggtccgagttccgcgcgtacgggctgcNeoR/KanR9,4609,4809,5009,5209,5409,5609,5809,6009,620
    gcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcgctcctagagcagcactgggtaccgctacggacgaacggcttatagtaccaccttttaccggcgaaaagacctaagtagctgacaccggccgacccacaccgcctggcgatagtcctgtatcgcaaccgatgggcactataacgacttctcgaaccgccgcttacccgactggcNcoIRsrIINeoR/KanR9,6409,6609,6809,7009,7209,7409,7609,7809,800
    cttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggctgaaggagcacgaaatgccatagcggcgagggctaagcgtcgcgtagcggaagatagcggaagaactgctcaagaagactcgccctgagaccccaagctttactggctggttcgctgcgggttggacggtagtgctctaaagctaaggtggcggcggaagatactttccaacccgaNeoR/KanR9,8209,8409,8609,8809,9009,9209,9409,960
    tcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttagccttagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaaSV40 poly(A) signal9,98010,00010,02010,04010,06010,08010,10010,12010,140
    gtccaaactcatcaatgtatcttagcggccgccaccgcggtggagctccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtcaggtttgagtagttacatagaatcgccggcggtggcgccacctcgaggtcgaaaacaagggaaatcactcccaattaacgcgcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacaAleINotIBstXISacIIlac_promoterSV40 poly(A) signalM13_pUC_rev_primerT3_promoterM13_re...rimer10,16010,18010,20010,22010,24010,26010,28010,30010,320
    aaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgctttcggaccccacggattactcactcgattgagtgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacg10,34010,36010,38010,40010,42010,44010,46010,48010,500
    gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagccagcaagccgacgccgctcgccatagtcgagtgagtttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactColE1 origin10,52010,54010,56010,58010,60010,62010,64010,660
    cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagcgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgColE1 origin10,68010,70010,72010,74010,76010,78010,80010,82010,840
    tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtApaLIColE1 origin10,86010,88010,90010,92010,94010,96010,98011,00011,020
    gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgatgccgatgtgatcttcctgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaColE1 origin11,04011,06011,08011,10011,12011,14011,16011,18011,200
    ttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagtColE1 origin11,22011,24011,26011,28011,30011,32011,34011,360
    atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgctagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgAhdIAmpicillin11,38011,40011,42011,44011,46011,48011,50011,52011,540
    gagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgAmpicillin11,56011,58011,60011,62011,64011,66011,68011,70011,720
    tacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaatacAmpicillin11,74011,76011,78011,80011,82011,84011,86011,88011,900
    gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcacgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtAmpicillin11,92011,94011,96011,98012,00012,02012,04012,060
    ttggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgApaLIAmpicillin12,08012,10012,12012,14012,16012,18012,20012,22012,240
    acggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataAmpR_promoterAmpicillin12,26012,28012,30012,32012,340
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    12346 bp90001000011000100020003000CAG_enhancerfillerM13_forward20_primerlacZ_a,M13_pUC_fwd_primer,T7_promoter,AloI,DraIIIPspOMIApaIhU6-F,U6 promoter,LKO.1 5',BsmBI,Esp3I,BaeIMfeIBspDI,ClaIBstAPISwaINsiI,PsrIgRNA scaffold,BsmBI,Esp3I,MluI,SpeICMV_immearly_promoterNeoR/KanR,NcoIRsrIISV40 poly(A) signalNotIT3_promoter,M13_reverse_primer,M13_pUC_rev_primer,lac_promoter,AleI,+2ColE1 origin,ApaLIAhdIAmpicillin,AmpR_promoter,ApaLI

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    12346