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pBT137-splitC 7.101 phage backbone
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cttAgaagagcTAttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccgaaTcttctcgATaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcaggSapIBspQIPflMIaph (KanR)20406080100120140160
    aacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggtttggcNsiIaph (KanR)180200220240260280300320340
    ttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggcccctagcgtcAjuIBsmIBsmINciINciIPvuISmaIXmaIAsiSIBsiEIBsmBIBsrFIEcoNIEsp3ITspMIaph (KanR)360380400420440460480500520
    tggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagataccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatgttagctatctaClaINsiIBspDIaph (KanR)540560580600620640660680700
    tgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaAggccaaataacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaagtactTccggtttatBglINruISfiIBanIIBspHIaph (KanR)NOT NECESSARILY SUFFIC... - context-dependent?720740760780800820840860880
    ggccgTtcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttccggcAagtctaggaaggcataaatcggtcatacaagagatcacaccaagcaacaaaaacgcactcggtactcttgcttggtaactctagtacgaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtagcacatcacaaaaagaa*I316RG314YK312AL310IN308EL306TT304RQ302KQ300TL298WS296FS294GN292LD290HK288SQ286MD284SL282FK280AE278FS276TL274QI272KA270TL268AD266HL264TK262R897-1847repA9009209409609801,0001,0201,0401,060
    agtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgttcaggcaatgcatccatccttagactacattaccaacaaccataaaacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctctaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttggtggttaaagtataacgacaBpuEISnaBIL260GN258RL256YS254DS252TI250TT248PI246KD244GN242MK240IQ238NN236EL234ET232VL230DM228QR226DL224EV222QF220IL218TD216TP214RG212RK210DV208VL206KM204NS202Y897-1847repA1,0801,1001,1201,1401,1601,1801,2001,2201,240
    aagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagattcacaaatttagaaatgaataaccaaagttttgggtaaccaattcggaaaatttgagtaccatcaataaaagttcgtaattgtacttgaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattattggtgagtatttaggagtatctMslIT200NL198DK196SI194PK192LV190WQ188NL186RK184FE182HY180NN178EL176ML174MF172KF170ED168LS166IE164IN162AK160HT158KK156QT154LE152KL150LW148EY146IR144MS142897-1847repA1,2601,2801,3001,3201,3401,3601,3801,4001,420
    gtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttcccataaacaaaagttttctgaattgtacaaggtctaatataaaatacttaaaaaaattgaccttttctattccgttatagagaagtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttagagtttcggaaattggtttcctaaggactaaaggBpuEIYK140NE138FS136KV134HE132LN130YK128IF126KK124LQ122FL120YP118LI116EE114SF112VL110EL108KE106SS104FK102AY100NT98WQ96FI94EF92GK90VL88PN86RI84E897-1847repA1,4401,4601,4801,5001,5201,5401,5601,5801,600
    acagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggttttgtcaagagcagtagtcgagagaccaacgaaatcgattatgtggtattcgtaaaagggatgactacaagtagtagactcgcataaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatcacggtgtgtcgtattttaatcgaaccaaaV82TR80TM78LE76RT74AK72AL70VG68YA66NE64RS62IN60MM58QA56YQ54NY52TF50SV48TR46EK44RT42PN40EI38TP36NL34LA32VC30CL28IL26KT24E897-1847repA1,6201,6401,6601,6801,7001,7201,7401,7601,780
    catgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcctttgagttgtgggtatctgtaaattctgctagtacgaggcaattcagtatcgctgattagcgatcaagtaaacgaaacttttgttgattaagtctgtatgtagagttaaccagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaggaaactcaacacccatagacatttaagacgatMfeIMfeISpeIH22ET20LD18YR16SI14AL12EN10AK8FV6VL4ES2M897-1847pSC101 orirepA1,8001,8201,8401,8601,8801,9001,9201,940
    gacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgctggaaacgaccttttgaacatttaagacgatctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggtcgggacacatattgagtgatgaaatcagtcaaggcBseYIpSC101 ori1,9601,9802,0002,0202,0402,0602,0802,1002,120
    cagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcgtcataatgttttcctacagcgtttgcgacaaacgaggagatgttttgtctggaattttgggatttccgaattcatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggactcagcgacagaaaaagcactgtaagtcaagcgacgcgagBcgIAflIIBseRIpSC101 oriparti...ding2,1402,1602,1802,2002,2202,2402,2602,2802,300
    acggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgatttgccgagaccgtcacttacccccatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacgatagactgaaaaacgacaagtcgtcaaggacgggagactaaKasINarISfcISfoIAlwNIBsaHIPluTIBstAPIpSC101 oripartitioning locus, gyrase bindinghairpin2,3202,3402,3602,3802,4002,4202,4402,4602,480
    ttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtcgctcttctGGCtctagaaggagatAttcaacatgctccctcaatcggttgaatgtcgcccttttgtctttggcgaaggtcagactggtgaagcctaatagggcactgtccagtaagtctgaccgattacgtgggtcattccgtcgccatagtagttgtccgaatgggcagaatgacagcgagaagaCCGagatcttcctctaTaagttgtacgagggagttagccaacttacagcgggaaaacagaaaccgcBmrISapIXbaIBspQIMspA1IpSC101 orifragment of gIII2,5002,5202,5402,5602,5802,6002,6202,6402,660
    ctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtTtttgcgtttcttttatatgttgccacctttatgtatgtatCttctacgtttgctaacatactgcgtaataaggagtcttaatcatgccagttcttttgggtattccgttattattgcgtgaccatttggtatacttaaaagataactaacactgttttatttgaataaggcaccacaAaaacgcaaagaaaatatacaacggtggaaatacatacataGaagatgcaaacgattgtatgacgcattattcctcagaattagtacggtcaagaaaacccataaggcaataataacgcaBtgIMslINdeIfragment of gIIIgVI promotergVI2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ttcctcggtttccttctggtaactttgttcggctatctgcttacttttctCaaaaagggcttcggtaagatagctattgctatttcattgtttcttgctcttattattgggcttaactcaattcttgtgggttatctctctgatattagTgctcaattaccctctgactttgttcaggaaggagccaaaggaagaccattgaaacaagccgatagacgaatgaaaagaGtttttcccgaagccattctatcgataacgataaagtaacaaagaacgagaataataacccgaattgagttaagaacacccaatagagagactataatcAcgagttaatgggagactgaaacaagtccCspCIgVI2,8602,8802,9002,9202,9402,9602,9803,0003,020
    gtgttcagttaattctcccgtctaatgcgcttccctgtttttatgttattctTtctgtaaagActgctattttcatttttgacgttaaacaaaaaatcgtttcttatttggattgggaCaaataatatggctgtttattttgtaactggcaaattaggctctggaaagacgctcgttacacaagtcaattaagagggcagattacgcgaagggacaaaaatacaataagaAagacatttcTgacgataaaagtaaaaactgcaatttgttttttagcaaagaataaacctaaccctGtttattataccgacaaataaaacattgaccgtttaatccgagacctttctgcgagcaatHgaIgVIgIgI promoter3,0403,0603,0803,1003,1203,1403,1603,1803,200
    gcgttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaatcttgatttaaggcttcaaaacctcccgcaagtcgggaggttcgctaaaacgcctcgcgttcttagaataccggataagccttctatatctgatttgcttgctattgggcgcggtaatgattcctacgcaaccattctaagtcctattttaacatcgacccacgttttatcgttgattagaactaaattccgaagttttggagggcgttcagccctccaagcgattttgcggagcgcaagaatcttatggcctattcggaagatatagactaaacgaacgataacccgcgccattactaaggatBseYIgI3,2203,2403,2603,2803,3003,3203,3403,3603,380
    cgatgaaaataaaaacggAttgcttgttctcgatgagtgcggtacttggtttaatacccgttcttggaatgataaggaaagacagccgattattgattggtttctacatgctcgtaaattaggatgggatattatttttcttgttcaggacttatctattgttgataaacaggcgcgtgctacttttatttttgccTaacgaacaagagctactcacgccatgaaccaaattatgggcaagaaccttactattcctttctgtcggctaataactaaccaaagatgtacgagcatttaatcctaccctataataaaaagaacaagtcctgaatagataacaactatttgtccgcgcaAloIgI3,4003,4203,4403,4603,4803,5003,5203,5403,560
    tctgcattagctgaacatgttgtttattgtcgtcgtctggacagaattactttaccttttgtcggtactttatattctcttattactggctcgaaaatgcctctgcctaaattacatgttggcgttgttaaatatggcgattctcaattaagccctactgttgagcgttggctttataagacgtaatcgacttgtacaacaaataacagcagcagacctgtcttaatgaaatggaaaacagccatgaaatataagagaataatgaccgagcttttacggagacggatttaatgtacaaccgcaacaatttataccgctaagagttaattcgggatgacaactcgcaaccgaaatatHpy99IgI3,5803,6003,6203,6403,6603,6803,7003,720
    ctggtaagaatttgtataacgcataCgatactaaacaggctttttctagtaattatgattccggtgtttattcttatttaacgccttatttatcacacggtcggtatttcaaaccattaaatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttctcgcgttctgaccattcttaaacatattgcgtatGctatgatttgtccgaaaaagatcattaatactaaggccacaaataagaataaattgcggaataaatagtgtgccagccataaagtttggtaatttaaatccagtcttctactttaattgattttatataaactttttcaaaagagcgcaagaBsiEIgIgXI3,7403,7603,7803,8003,8203,8403,8603,8803,900
    ttgtcttgcgattggatttgcatcagcatttacatatagttatataacccaacctaagccggaggttaaaaaggtagtctctcagacctatgattttgataaattcactattgactcttctcaTcgtcttaatctaagctatcgctatgttttcaaggattctaagggaaaattaattaacagaacgctaacctaaacgtagtcgtaaatgtatatcaatatattgggttggattcggcctccaatttttccatcagagagtctggatactaaaactatttaagtgataactgagaagagtAgcagaattagattcgatagcgatacaaaagttcctaagattcccttttaattaaPacIgIgXIgIV promoter3,9203,9403,9603,9804,0004,0204,0404,0604,080
    aatagcgacgatttacagaagcaaggttattcactTacatatattgatttatgtactgtttccattaaaaaaggtaattcaaatgaaattgttaaatgtaattaattttgttttcttgatgtttgtttcatcatcttcttttgctcaggtaattgaaatgaataattcgcctctgcgcttatcgctgctaaatgtcttcgttccaataagtgaAtgtatataactaaatacatgacaaaggtaattttttccattaagtttactttaacaatttacattaattaaaacaaaagaactacaaacaaagtagtagaagaaaacgagtccattaactttacttattaagcggagacgcgTatIHpy99IgIgXIgIV4,1004,1204,1404,1604,1804,2004,2204,2404,260
    gattttAtaacttggtattcaaagcaatcaggcgaatccgttattgtttctcccgatgtaaaaggtactgttactgtatattcatctgacgttaaaTctgaaaatctacgcaatttctttatttctgttttacgtgcaaataattttgatatggtaggttctaacccttccattattcctaaaaTattgaaccataagtttcgttagtccgcttaggcaataacaaagagggctacattttccatgacaatgacatataagtagactgcaatttAgacttttagatgcgttaaagaaataaagacaaaatgcacgtttattaaaactataccatccaagattgggaaggtaataaggIV4,2804,3004,3204,3404,3604,3804,4004,4204,440
    agaagtataatccaaacaatcaggattatattgatgaattgccatcaCctgataatcaggaatatgatgataattccgctccttctggtggtttctttgtCccgcaaaatgataatgttactcaaacttttaaaattaataacgttcgggcaaaggatttaCtacgagttgtcgaatttcttcatattaggtttgttagtcctaatataactacttaacggtagtGgactattagtccttatactactattaaggcgaggaagaccaccaaagaaacaGggcgttttactattacaatgagtttgaaaattttaattattgcaagcccgtttcctaaatGatgctcaacagcttaaAclIgIV4,4604,4804,5004,5204,5404,5604,5804,6004,620
    gtttgtaaagtctaatacttctaaatcctcaaatgtattatctattgacggAtctaatctattagttgttagtgctcctaaagatattttagataaccttTctcaattcctttcaactgttgatttgccaactgaccagGtattgattgagggtttgatatttgaggttcagcaaggtcaaacatttcagattatgaagatttaggagtttacataatagataactgccTagattagataatcaacaatcacgaggatttctataaaatctattggaaAgagttaaggaaagttgacaactaaacggttgactggtcCataactaactcccaaactataaactccaagtcgttccaSexAIgIV4,6404,6604,6804,7004,7204,7404,7604,7804,800
    gatgctttagatttttcatttgctgctggctctcagcgtggcactgttgcaggcggAgttaatactgaccgcctcacctctgttttatcttctgctggtggttcgttcggtatttttaatggcgatgttttagggctatcagttcAcgcattaaagactaatagccattcaaaaatatctacgaaatctaaaaagtaaacgacgaccgagagtcgcaccgtgacaacgtccgccTcaattatgactggcggagtggagacaaaatagaagacgaccaccaagcaagccataaaaattaccgctacaaaatcccgatagtcaagTgcgtaatttctgattatcggtaagtttttataEciIBtgZIgIV4,8204,8404,8604,8804,9004,9204,9404,9604,980
    tgtctgtgccacgtattcttacgctttcaggtcagaagggttctatctctAttggccagaatgtcccttttattactggtcgtgtAactggtgaatctgccaatgtaaataatccatttcagacgattgagcgtcaaaatgtaggtatttccatgagcgtttttcctgttgcaatggcacagacacggtgcataagaatgcgaaagtccagtcttcccaagatagagaTaaccggtcttacagggaaaataatgaccagcacaTtgaccacttagacggttacatttattaggtaaagtctgctaactcgcagttttacatccataaaggtactcgcaaaaaggacaacgttaccgEaeIHgaIMscIgIV5,0005,0205,0405,0605,0805,1005,1205,1405,160
    tggcggtaatattgttctggatattaccagcaaggccgatagtttgagttcttctactcaggcaagtgatgttattactaaCcaaagaagtattgctacaacggttaatttgcgtgatggacagactcttCtactcggtggcctcactgattataaaaacacttctcaggattctggcaccgccattataacaagacctataatggtcgttccggctatcaaactcaagaagatgagtccgttcactacaataatgattGgtttcttcataacgatgttgccaattaaacgcactacctgtctgagaaGatgagccaccggagtgactaatatttttgtgaagagtcctaagaccgBarIBsrDIgIV5,1805,2005,2205,2405,2605,2805,3005,3205,340
    gtaccgttcctgtctaaaatccctttaatcggcctcctgtttagctcccgctctgaCtctaacgaggaGagcacgttatacgtgctcgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagccatggcaaggacagattttagggaaattagccggaggacaaatcgagggcgagactGagattgctcctCtcgtgcaatatgcacgagcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgSfcIBsaXIBseRIgIVpackaging signal5,3605,3805,4005,4205,4405,4605,4805,500
    gccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagcGctaaatcgggggcccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatacgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgCgatttagcccccggggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatAfeIApaINaeIBaeGIBanIIBsrFIBtgZIDraIIINgoMIVPspOMIEcoO109IEcoO109IRNA pri...cationpack...nal5,5205,5405,5605,5805,6005,6205,6405,6605,680
    gacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaaAloIDrdIBsaXIcomplete (+) originessential (+) origin5,7005,7205,7405,7605,7805,8005,8205,8405,860
    aacaaaatattaacgtttacaatttaaatatttgcttatacaatcttcctgtttttggggcttttttgttttataattgcaaatgttaaatttataaacgaatatgttagaaggacaaaaaccccgaaaaAclISwaIgII promoter5,8805,9005,920
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    5939 bpSapI Golden Gate dropout cassette: ori, KmR425045004750500052505500575025050075010001250gIVrepAaph (KanR)nicking site897-1847CTT for SapI Golden Gate cloning,USER jct 18,SapI,TAAT stop,BspQI,SapI,PflMI,NsiIAjuI,BsmBI,Esp3I,AsiSI,BsiEI,PvuI,BsmI,BsrFI,EcoNIBsmI,TspMI,XmaI,NciI,NciI,SmaI,NsiIBspDI,ClaI,NruI,BanIINOT NECESSARILY SUFFICIENT promoter - context-dependent?BspHIBglI,SfiISnaBIBpuEIMslImutated vs generation 1 backbonemutated vs generation 1 backbone,+3mutated vs generation 1 backbonemutated vs generation 1 backbone,+2mutated vs generation 1 backbone,EciIR259H gIV,mutated vs generation 2 backbone,BtgZImutated vs generation 1 backbone,EaeI,MscImutated vs generation 1 backbone,HgaI,BsrDImutated vs generation 1 backbone,mutated vs generation 1 backbone,BarID407 silent gIV,mutated vs generation 2 backbone,BseRI,BsaXIpackaging signal,mutated vs generation 1 backbone,SfcI,BsrFI,NgoMIV,NaeI,+7RNA primer for (-) replication,essential (+) origin,complete (+) origin,+8

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    Enzyme AatII
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    Sequence Alignments

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    Sequence Info

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    5939