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pBT137-splitC 7.101 phage backbone
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    cttAgaagagcTAttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcggaaTcttctcgATaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcSapIBspQIPflMIaph (KanR)20406080100120140160
    tccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggNsiIaph (KanR)180200220240260280300320340
    aaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggtttggcaataagtaagcactaacgcggactcgctctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggccAjuIBsmIBsmINciINciIPvuISmaIXmaIAsiSIBsiEIBsmBIBsrFIEcoNIEsp3ITspMIaph (KanR)360380400420440460480500520
    ggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatNsiIaph (KanR)540560580600620640660680700
    caatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaaClaINruIBanIIBspDIaph (KanR)NOT NECESSARILY SUFF... context-dependent?720740760780800820840860
    catgaAggccaaataggccgTtcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcagtactTccggtttatccggcAagtctaggaaggcataaatcggtcatacaagagatcacaccaagcaacaaaaacgcactcggtactcttgcttggtaactctagtacgaatgaaacgtacagtgagtttttaaaacggagttttgaccactcgacttaaaaacgtcaatttcgtBglISfiIBspHI*I316RG314YK312AL310IN308EL306TT304RQ302KQ300TL298WS296FS294GN292LD290HK288SQ286MD284SL282FK280AE278FS276TL274QI272KA270TL268A897-1847repA8809009209409609801,0001,0201,040
    tcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaagcacatcacaaaaagaatcaggcaatgcatccatccttagactacattaccaacaaccataaaacagtggtaagtaaaaatagaccaacaagagttcaagccaatgctctaggtaaacagatagatcaagttgaaccttttagttgcatagtcagcccgccggagcgaatagttBpuEISnaBID266HL264TK262RL260GN258RL256YS254DS252TI250TT248PI246KD244GN242MK240IQ238NN236EL234ET232VL230DM228QR226DL224EV222QF220IL218TD216TP214RG212RK210DV208897-1847repA1,0601,0801,1001,1201,1401,1601,1801,2001,220
    ccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaaggtggttaaagtataacgacattcacaaatttagaaatgaataaccaaagttttgggtaaccaattcggaaaatttgagtaccatcaataaaagttcgtaattgtacttgaatttaagtagttccgattagagatataaacggaacactcaaaagaaaacacaatcaagaaaattMslIVL206KM204NS202YT200NL198DK196SI194PK192LV190WQ188NL186RK184FE182HY180NN178EL176ML174MF172KF170ED168LS166IE164IN162AK160HT158KK156QT154LE152KL150897-1847repA1,2401,2601,2801,3001,3201,3401,3601,3801,400
    taaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagattggtgagtatttaggagtatctcataaacaaaagttttctgaattgtacaaggtctaatataaaatacttaaaaaaattgaccttttctattccgttatagagaagtgatttttgattaagattaaaaagcgaactcttgaaccgtatcaaacaggtgaccttttagagtttcBpuEILW148EY146IR144MS142YK140NE138FS136KV134HE132LN130YK128IF126KK124LQ122FL120YP118LI116EE114SF112VL110EL108KE106SS104FK102AY100NT98WQ96FI94EF92897-1847repA1,4201,4401,4601,4801,5001,5201,5401,560
    cctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtaggaaattggtttcctaaggactaaaggtgtcaagagcagtagtcgagagaccaacgaaatcgattatgtggtattcgtaaaagggatgactacaagtagtagactcgcataaccaatattcacttgctatggcaggcaagaaaggaacatcccaaaagttagcaccccaactcatGK90VL88PN86RI84EV82TR80TM78LE76RT74AK72AL70VG68YA66NE64RS62IN60MM58QA56YQ54NY52TF50SV48TR46EK44RT42PN40EI38TP36NL34L897-1847repA1,5801,6001,6201,6401,6601,6801,7001,7201,740
    gtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattactagtccttttcacggtgtgtcgtattttaatcgaaccaaagtacgaggcaattcagtatcgctgattagcgatcaagtaaacgaaacttttgttgattaagtctgtatgtagagttaaccagatccactaaaattagtgatatggttaactctacccgatcagttactattaatgatcaggaaaaMfeIMfeISpeIA32VC30CL28IL26KT24EH22ET20LD18YR16SI14AL12EN10AK8FV6VL4ES2M897-1847repApSC101 ori1,7601,7801,8001,8201,8401,8601,8801,9001,920
    cctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccggaaactcaacacccatagacatttaagacgatctggaaacgaccttttgaacatttaagacgatctgggagacatttaaggcgatctggaaacacacaaaaaaaacaaatataagttcaccaatattaaatatcttatttctttcttatttttttctatttttcttatctagggBseYIpSC101 ori1,9401,9601,9802,0002,0202,0402,0602,0802,100
    agccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgtcgggacacatattgagtgatgaaatcagtcaaggcgtcataatgttttcctacagcgtttgcgacaaacgaggagatgttttgtctggaattttgggatttccgaattcatcgtgggagcgttcgagcccgtttagcgacttataaggaaaacagaggctggtagtccgtggacAflIIBseRIpSC101 ori2,1202,1402,1602,1802,2002,2202,2402,260
    agtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgtcagcgacagaaaaagcactgtaagtcaagcgacgcgagtgccgagaccgtcacttacccccatttaccgtgatgtccgcggaaaatacctaagtacgttcctttgatgggtattatgttcttttcgggcagtgcccgaagagtcccgcaaaataccgcccagacgatacaccacBcgIKasINarISfcISfoIBsaHIPluTIpSC101 oripartitioning locus, gyrase bindinghairpin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    ctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtcgctcttctGGCtctagaaggagatAttcagatagactgaaaaacgacaagtcgtcaaggacgggagactaaaaggtcagactggtgaagcctaatagggcactgtccagtaagtctgaccgattacgtgggtcattccgtcgccatagtagttgtccgaatgggcagaatgacagcgagaagaCCGagatcttcctctaTaagtBmrISapIXbaIAlwNIBspQIBstAPIMspA1IpSC101 oripartitionin...se binding2,4602,4802,5002,5202,5402,5602,5802,6002,620
    acatgctccctcaatcggttgaatgtcgcccttttgtctttggcgctggtaaaccatatgaattttctattgattgtgacaaaataaacttattccgtggtgtTtttgcgtttcttttatatgttgccacctttatgtatgtatCttctacgtttgctaacatactgcgtaataatgtacgagggagttagccaacttacagcgggaaaacagaaaccgcgaccatttggtatacttaaaagataactaacactgttttatttgaataaggcaccacaAaaacgcaaagaaaatatacaacggtggaaatacatacataGaagatgcaaacgattgtatgacgcattattBtgIMslINdeIfragment of gIIIgVI promoter2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ggagtcttaatcatgccagttcttttgggtattccgttattattgcgtttcctcggtttccttctggtaactttgttcggctatctgcttacttttctCaaaaagggcttcggtaagatagctattgctatttcattgtttcttgctcttattattgggcttaactcaattcttgcctcagaattagtacggtcaagaaaacccataaggcaataataacgcaaaggagccaaaggaagaccattgaaacaagccgatagacgaatgaaaagaGtttttcccgaagccattctatcgataacgataaagtaacaaagaacgagaataataacccgaattgagttaagaacCspCIgVIfr...I2,8202,8402,8602,8802,9002,9202,9402,960
    tgggttatctctctgatattagTgctcaattaccctctgactttgttcagggtgttcagttaattctcccgtctaatgcgcttccctgtttttatgttattctTtctgtaaagActgctattttcatttttgacgttaaacaaaaaatcgtttcttatttggattgggaCaaataacccaatagagagactataatcAcgagttaatgggagactgaaacaagtcccacaagtcaattaagagggcagattacgcgaagggacaaaaatacaataagaAagacatttcTgacgataaaagtaaaaactgcaatttgttttttagcaaagaataaacctaaccctGtttatgVIgI promoter2,9803,0003,0203,0403,0603,0803,1003,1203,140
    atatggctgtttattttgtaactggcaaattaggctctggaaagacgctcgttagcgttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaatcttgatttaaggcttcaaaacctcccgcaagtcgggaggttcgctaaaacgcctcgcgttcttagaattataccgacaaataaaacattgaccgtttaatccgagacctttctgcgagcaatcgcaaccattctaagtcctattttaacatcgacccacgttttatcgttgattagaactaaattccgaagttttggagggcgttcagccctccaagcgattttgcggagcgcaagaatcttaHgaIBseYIgI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    accggataagccttctatatctgatttgcttgctattgggcgcggtaatgattcctacgatgaaaataaaaacggAttgcttgttctcgatgagtgcggtacttggtttaatacccgttcttggaatgataaggaaagacagccgattattgattggtttctacatgctcgtaaatggcctattcggaagatatagactaaacgaacgataacccgcgccattactaaggatgctacttttatttttgccTaacgaacaagagctactcacgccatgaaccaaattatgggcaagaaccttactattcctttctgtcggctaataactaaccaaagatgtacgagcatttAloIgI3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ttaggatgggatattatttttcttgttcaggacttatctattgttgataaacaggcgcgttctgcattagctgaacatgttgtttattgtcgtcgtctggacagaattactttaccttttgtcggtactttatattctcttattactggctcgaaaatgcctctgcctaaattacaatcctaccctataataaaaagaacaagtcctgaatagataacaactatttgtccgcgcaagacgtaatcgacttgtacaacaaataacagcagcagacctgtcttaatgaaatggaaaacagccatgaaatataagagaataatgaccgagcttttacggagacggatttaatgHpy99IgI3,5203,5403,5603,5803,6003,6203,6403,660
    atgttggcgttgttaaatatggcgattctcaattaagccctactgttgagcgttggctttatactggtaagaatttgtataacgcataCgatactaaacaggctttttctagtaattatgattccggtgtttattcttatttaacgccttatttatcacacggtcggtatttcaatacaaccgcaacaatttataccgctaagagttaattcgggatgacaactcgcaaccgaaatatgaccattcttaaacatattgcgtatGctatgatttgtccgaaaaagatcattaatactaaggccacaaataagaataaattgcggaataaatagtgtgccagccataaagttBsiEIgI3,6803,7003,7203,7403,7603,7803,8003,8203,840
    accattaaatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttctcgcgttctttgtcttgcgattggatttgcatcagcatttacatatagttatataacccaacctaagccggaggttaaaaaggtagtctctcagacctatgattttgataaattcacttggtaatttaaatccagtcttctactttaattgattttatataaactttttcaaaagagcgcaagaaacagaacgctaacctaaacgtagtcgtaaatgtatatcaatatattgggttggattcggcctccaatttttccatcagagagtctggatactaaaactatttaagtgagIgXIgIV ...ter3,8603,8803,9003,9203,9403,9603,9804,0004,020
    attgactcttctcaTcgtcttaatctaagctatcgctatgttttcaaggattctaagggaaaattaattaatagcgacgatttacagaagcaaggttattcactTacatatattgatttatgtactgtttccattaaaaaaggtaattcaaatgaaattgttaaatgtaattaattaactgagaagagtAgcagaattagattcgatagcgatacaaaagttcctaagattcccttttaattaattatcgctgctaaatgtcttcgttccaataagtgaAtgtatataactaaatacatgacaaaggtaattttttccattaagtttactttaacaatttacattaattaPacITatIHpy99IgIgXIgIV promotergIV4,0404,0604,0804,1004,1204,1404,1604,1804,200
    tttgttttcttgatgtttgtttcatcatcttcttttgctcaggtaattgaaatgaataattcgcctctgcgcgattttAtaacttggtattcaaagcaatcaggcgaatccgttattgtttctcccgatgtaaaaggtactgttactgtatattcatctgacgttaaaTctgaaaaaacaaaagaactacaaacaaagtagtagaagaaaacgagtccattaactttacttattaagcggagacgcgctaaaaTattgaaccataagtttcgttagtccgcttaggcaataacaaagagggctacattttccatgacaatgacatataagtagactgcaatttAgactttgIV4,2204,2404,2604,2804,3004,3204,3404,360
    atctacgcaatttctttatttctgttttacgtgcaaataattttgatatggtaggttctaacccttccattattcagaagtataatccaaacaatcaggattatattgatgaattgccatcaCctgataatcaggaatatgatgataattccgctccttctggtggtttctttgttagatgcgttaaagaaataaagacaaaatgcacgtttattaaaactataccatccaagattgggaaggtaataagtcttcatattaggtttgttagtcctaatataactacttaacggtagtGgactattagtccttatactactattaaggcgaggaagaccaccaaagaaacagIV4,3804,4004,4204,4404,4604,4804,5004,5204,540
    CccgcaaaatgataatgttactcaaacttttaaaattaataacgttcgggcaaaggatttaCtacgagttgtcgaattgtttgtaaagtctaatacttctaaatcctcaaatgtattatctattgacggAtctaatctattagttgttagtgctcctaaagatattttagataacGggcgttttactattacaatgagtttgaaaattttaattattgcaagcccgtttcctaaatGatgctcaacagcttaacaaacatttcagattatgaagatttaggagtttacataatagataactgccTagattagataatcaacaatcacgaggatttctataaaatctattgAclIgIV4,5604,5804,6004,6204,6404,6604,6804,7004,720
    cttTctcaattcctttcaactgttgatttgccaactgaccagGtattgattgagggtttgatatttgaggttcagcaaggtgatgctttagatttttcatttgctgctggctctcagcgtggcactgttgcaggcggAgttaatactgaccgcctcacctctgttttatcttctggaaAgagttaaggaaagttgacaactaaacggttgactggtcCataactaactcccaaactataaactccaagtcgttccactacgaaatctaaaaagtaaacgacgaccgagagtcgcaccgtgacaacgtccgccTcaattatgactggcggagtggagacaaaatagaagacEciISexAIgIV4,7404,7604,7804,8004,8204,8404,8604,8804,900
    ctggtggttcgttcggtatttttaatggcgatgttttagggctatcagttcAcgcattaaagactaatagccattcaaaaatattgtctgtgccacgtattcttacgctttcaggtcagaagggttctatctctAttggccagaatgtcccttttattactggtcgtgtAactgggaccaccaagcaagccataaaaattaccgctacaaaatcccgatagtcaagTgcgtaatttctgattatcggtaagtttttataacagacacggtgcataagaatgcgaaagtccagtcttcccaagatagagaTaaccggtcttacagggaaaataatgaccagcacaTtgaccEaeIMscIBtgZIgIV4,9204,9404,9604,9805,0005,0205,0405,060
    tgaatctgccaatgtaaataatccatttcagacgattgagcgtcaaaatgtaggtatttccatgagcgtttttcctgttgcaatggctggcggtaatattgttctggatattaccagcaaggccgatagtttgagttcttctactcaggcaagtgatgttattactaaCcaaagaacttagacggttacatttattaggtaaagtctgctaactcgcagttttacatccataaaggtactcgcaaaaaggacaacgttaccgaccgccattataacaagacctataatggtcgttccggctatcaaactcaagaagatgagtccgttcactacaataatgattGgtttctBarIHgaIBsrDIgIV5,0805,1005,1205,1405,1605,1805,2005,2205,240
    agtattgctacaacggttaatttgcgtgatggacagactcttCtactcggtggcctcactgattataaaaacacttctcaggattctggcgtaccgttcctgtctaaaatccctttaatcggcctcctgtttagctcccgctctgaCtctaacgaggaGagcacgttatacgtgctcataacgatgttgccaattaaacgcactacctgtctgagaaGatgagccaccggagtgactaatatttttgtgaagagtcctaagaccgcatggcaaggacagattttagggaaattagccggaggacaaatcgagggcgagactGagattgctcctCtcgtgcaatatgcacgBseRIgIV5,2605,2805,3005,3205,3405,3605,3805,4005,420
    tcgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagcGctaaatcgggagcagtttcgttggtatcatgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgCgatttagcccAfeINaeISfcIBsaXIBsrFINgoMIVpackaging signalgIV5,4405,4605,4805,5005,5205,5405,5605,5805,600
    ggcccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaacccggggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaaacccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgAloIApaIDrdIBaeGIBanIIBsaXIBtgZIDraIIIPspOMIEcoO109IEcoO109Icomplete (+) originessential (+) originRNA pri...cation5,6205,6405,6605,6805,7005,7205,7405,760
    cctatctcgggctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttaaatatttgcttatacaatcttcctgtttttggggcttttggatagagcccgataagaaaactaaatattccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaatttataaacgaatatgttagaaggacaaaaaccccgaaaaAclISwaIcomplete (+) origingII promoter5,7805,8005,8205,8405,8605,8805,9005,920
    • Plasmid
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    • Description
    • Linear Map
    Editing is disabled because sequence edits have been locked.
    5939 bpSapI Golden Gate dropout cassette: ori, KmR425045004750500052505500575025050075010001250gIVrepAaph (KanR)nicking site897-1847CTT for SapI Golden Gate cloning,USER jct 18,SapI,TAAT stop,BspQI,SapI,PflMI,NsiIAjuI,BsmBI,Esp3I,AsiSI,BsiEI,PvuI,BsmI,BsrFI,EcoNIBsmI,TspMI,XmaI,NciI,NciI,SmaI,NsiIBspDI,ClaI,NruI,BanIINOT NECESSARILY SUFFICIENT promoter - context-dependent?BspHIBglI,SfiISnaBIBpuEIMslImutated vs generation 1 backbonemutated vs generation 1 backbone,+3mutated vs generation 1 backbonemutated vs generation 1 backbone,+2mutated vs generation 1 backbone,EciIR259H gIV,mutated vs generation 2 backbone,BtgZImutated vs generation 1 backbone,EaeI,MscImutated vs generation 1 backbone,HgaI,BsrDImutated vs generation 1 backbone,mutated vs generation 1 backbone,+1D407 silent gIV,mutated vs generation 2 backbone,BseRI,BsaXIpackaging signal,mutated vs generation 1 backbone,SfcI,BsrFI,NgoMIV,NaeI,+7RNA primer for (-) replication,essential (+) origin,complete (+) origin,+8

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    Selected Color
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    Primers

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    Primer Position Product Size

    History

    Lineage

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    Sequence Alignments

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    Sequence Info

    Tags

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    Bases
    5939