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EW428 CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deImmLink-ZIK1KRAB
  • Sequence Map
    Editing disabled on read-only sequence.
    gttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatcaactgtaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttaSpeICMV20406080100120140160
    agggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcgggggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagBmrINdeICMV180200220240260280300320340
    tacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccatatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaNcoISnaBICMV360380400420440460480500520
    tgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctSacISacIBsaBIBsaBIBsmBIEsp3ISgrDIEco53kIEco53kICMVTetR binding sitesTetOTetO540560580600620640660680700
    ccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGTGCCAAggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCACGGTTPmeIAflIIHindIIIM1YE2CN4EC6GK8TF10TR12SS14NL16IV18HQ20RI22HT24GE26KP28FA30CN32DC34GK36AF38TQ40SA42N763-1098ZNF35 ZF Domains 5-8720740760780800820840860880
    CTTGATCGTGCATCAGAGAAGCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGATTGTGGAACTAGCACGTAGTCTCTTCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACTAACACBsrGIL44IV46HQ48RS50HT52GE54KP56YE58CK60EC62GK64AF66SC68FS70HL72IV74HQ76RI78HT80AE82KP84YD86CS88EC90GK92AF94SQ96LS98CL100IV102763-1098ZNF35 ZF Domains 5-89009209409609801,0001,0201,0401,060
    CATCAGAGAATTCACACGGGCGAACGCCCCTACGAGTGTAATGAGTGCGGTAAGACCTTCACACGATCTTCTAACCTTATTGTGCATCAACGAATCCATACAGGCGAGAAGCCCTTTGCCTGCAATGACTGTGGGAAGGCTTTTACTCAGAGTGCCAACTTGATCGTGCATCAGAGAAGTAGTCTCTTAAGTGTGCCCGCTTGCGGGGATGCTCACATTACTCACGCCATTCTGGAAGTGTGCTAGAAGATTGGAATAACACGTAGTTGCTTAGGTATGTCCGCTCTTCGGGAAACGGACGTTACTGACACCCTTCCGAAAATGAGTCTCACGGTTGAACTAGCACGTAGTCTCTTYE2CN4EC6GK8TF10TR12SS14NL16IV18HQ20RI22HT24GE26KP28FA30CN32DC34GK36AF38TQ40SA42NL44IV46HQ48R1099-1419HQ104RI106HT108GE110RP112763-1098ZNF35 ZF Domains 5-8ZNF3...5-8Cano...ker1,0801,1001,1201,1401,1601,1801,2001,2201,240
    GCCATACGGGTGAGAAGCCCTATGAATGCAAGGAATGCGGAAAGGCCTTCTCCTGCTTCAGCCACCTCATTGTACACCAGCGCATACACACGGCTGAGAAGCCATACGATTGCTCTGAATGTGGAAAGGCCTTTAGTCAGCTGTCTTGCTTGATTGTGCATCAGAGAATTCACgctgcCGGTATGCCCACTCTTCGGGATACTTACGTTCCTTACGCCTTTCCGGAAGAGGACGAAGTCGGTGGAGTAACATGTGGTCGCGTATGTGTGCCGACTCTTCGGTATGCTAACGAGACTTACACCTTTCCGGAAATCAGTCGACAGAACGAACTAACACGTAGTCTCTTAAGTGcgacgBsrGIS50HT52GE54KP56YE58CK60EC62GK64AF66SC68FS70HL72IV74HQ76RI78HT80AE82KP84YD86CS88EC90GK92AF94SQ96LS98CL100IV102HQ104RI106H1099-1419A1A2ZNF35 ZF Domains 5-81,2601,2801,3001,3201,3401,3601,3801,4001,420
    tgcaTGGGAGCTAggaATGtctggcggatctTGCGTGACCTTTGAAGACATTGCCATCTATTTTAGTCAAGACGAATGGGGGCTCCTGGACGAAGCCCAGAGACTTCTGTACCTGGAGGTGATGCTTGAAAACTTCGCTCTCGTGGCATCCCTGtagggatcataggccgctcgaggaacgtACCCTCGATcctTACagaccgcctagaACGCACTGGAAACTTCTGTAACGGTAGATAAAATCAGTTCTGCTTACCCCCGAGGACCTGCTTCGGGTCTCTGAAGACATGGACCTCCACTACGAACTTTTGAAGCGAGAGCACCGTAGGGACatccctagtatccggcgagctcctAvaIBaeIPfoITliIXhoIBsoBIPspXIPaeR7IA3W4E5L6G7M8S9G10G11S121420-1455C1V2T3F4E5D6I7A8I9Y10F11S12Q13D14E15W16G17L18L19D20E21A22Q23R24L25L26Y27L28E29V30M31L32E33N34F35A36L37V38A39S40L41*421456-1581ZIK1 KRAB Domain1,4401,4601,4801,5001,5201,5401,5601,5801,600
    gggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgcccgggcttgtttttgagtagagtcttctcctagacttatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacApaIBclIPmeIBseRIPspOMIEcoO109IBGH polyadenylation sequencec-myc epitopePolyhi...e tag1,6201,6401,6601,6801,7001,7201,7401,7601,780
    tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctcaggaaaggattattttactcctttaacgtagcgtaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagClaINspISphIBspDIBGH polyadenylation sequenceBGH pA1,8001,8201,8401,8601,8801,9001,9201,940
    tatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccataccgaagactccgcctttcttggtcgaccccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggNgoMIV1,9601,9802,0002,0202,0402,0602,0802,1002,120
    ggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaacccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgBarIMscINaeIXbaIFRTHyg...in2,1402,1602,1802,2002,2202,2402,2602,2802,300
    tcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcgagtggcgctgcagacagctcttcaaagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagcBsaXIBsmBIEsp3IPflFIPshAITth111IHygromycin2,3202,3402,3602,3802,4002,4202,4402,4602,480
    gcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcgcgtgaaacgtagccggcgcgagggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgcNcoIPstIPvuIAsiSIBfuAIBspMIDraIIIHygromycin2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttcggctagaatcggtctgctcgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaaNdeIPflFIRsrIITth111IHygromycin2,6802,7002,7202,7402,7602,7802,8002,8202,840
    gggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggacccggctcctgacggggcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctAjuIDraIIINmeAIIIHygromycin2,8602,8802,9002,9202,9402,9602,9803,0003,020
    gcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggcgtcgtctgcgcgatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggcccSacIIHygromycin3,0403,0603,0803,1003,1203,1403,1603,1803,200
    actgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatgacagcccgcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttScaIHygromycin3,2203,2403,2603,2803,3003,3203,3403,3603,380
    tcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactagcaaaaggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgaNaeIPfoIPsiINgoMIVSV40 pA3,4003,4203,4403,4603,4803,5003,5203,5403,560
    catcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattagtagttacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaatBstZ17ISV40 pA3,5803,6003,6203,6403,6603,6803,7003,720
    attgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaataacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagttSapIBsaXIBspQI3,7403,7603,7803,8003,8203,8403,8603,8803,900
    aggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaaccctccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctttgggNspIPciIAflIIIpUC ori3,9203,9403,9603,9804,0004,0204,0404,0604,080
    gacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgaggttcgacpUC ori4,1004,1204,1404,1604,1804,2004,2204,2404,260
    ggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcaccaccggattgaAlwNIpUC ori4,2804,3004,3204,3404,3604,3804,4004,4204,440
    acggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgattgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttcttctaggaaactapUC ori4,4604,4804,5004,5204,5404,5604,5804,6004,620
    cttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggttacgaattagtcactcBspHIAmp (bla)pUC ori4,6404,6604,6804,7004,7204,7404,7604,7804,800
    gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgcgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttggtcggtcggccttcccggcAhdIBmrIBsaIAmp (bla)4,8204,8404,8604,8804,9004,9204,9404,9604,980
    agcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgtcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaagtaagtcgaggccaagggttgcAclIFspINmeAIIIAmp (bla)5,0005,0205,0405,0605,0805,1005,1205,1405,160
    atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattctacgaaaagacactgaccactcatgPvuIScaIAmp (bla)5,1805,2005,2205,2405,2605,2805,3005,3205,340
    tcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaatggcgacaactctaggtcaagctacattgAclIBcgIXmnIAmp (bla)5,3605,3805,4005,4205,4405,4605,4805,500
    ccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataataacttcgtaaatagtcccaataacagagtaSspIBspHIAmp (bla)bla promoter5,5205,5405,5605,5805,6005,6205,6405,6605,680
    gagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttctcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactacggcgtatcaattcggtcatagacgagggacgaaAlwNISgrDIbla promoter5,7005,7205,7405,7605,7805,8005,8205,8405,860
    gtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgccacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgMfeIMluIBsiWIAflIIIBpu10I5,8805,9005,9205,9405,9605,9806,000
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6007 bp45005000550050010001500ZIK1 KRAB DomainCMVTetR binding sitesTetO1456-1581EKbR (DNA)pNSK053 (DNA)FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)SpeI,NdeIBmrI,SnaBI,NcoITATA box,TetO,CMV-Forward Genewiz (DNA),Eco53kI,SacICMVF1 (DNA),BsaBI,BsaBI,SgrDI,Eco53kI,SacI,BsmBI,Esp3IKozak,start codon,760-762,ToFlpF (DNA),EWo300 (DNA),EKbF (DNA),+4EWo153.1 (DNA)ZNF35 ZF Domains 5-8,763-1098,EWo51 (DNA),BsrGIEWo126 (DNA)EWo169.1 (DNA)Canonical ZF Linker,EWo120.1 (DNA)ZNF35 ZF Domains 5-8,1099-1419BsrGIEWo169.1 (DNA),EWo196.1 (DNA)1420-1455EWo270.1 (DNA),EWo416 (DNA)BspHIAhdIBmrI,BsaINmeAIIIFspIAclIAmp (bla),PvuIScaI,BcgIAclI,XmnIbla promoter,SspI,BspHIFlpInF1 (DNA),FlpInF (DNA),SgrDI,AlwNI,MfeI,Bpu10I,BsiWI,AflIII,MluI

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    6007