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pBT100.136c FERNY-intein SapI Golden Gate donor
  • Sequence Map
    Editing is disabled because sequence edits have been locked.
    aatgctcttctagtgtacacactaccacgttaaggaggtaaagtatgtttgaacgaaattatgacccacgcgagctgcgcaaggaaacatatcttctttacgagattaagtggggcaagagtggtaaactctggaggcattggtgtcaaaacaatcgcactcaacatgcagaggtttacgagaagatcacatgtgtgatggtgcaattcctccatttcatacaaacttgctttaatactgggtgcgctcgacgcgttcctttgtatagaagaaatgctctaattcaccccgttctcaccatttgagacctccgtaaccacagttttgttagcgtgagttgtacgtctccaFspINspIRsaISapITatIBspQIBsrGICviQIMF2ER4NY6DP8RE10LR12KE14TY16LL18YE20IK22WG24KS26GK28LW30RH32WC34QN36NR38TQ40HA42EV4445-530FERNYsyn RBS 130k init20406080100120140160
    gtatttcctggaaaacatttttaacgctcgccgctttaatccatcaacgcattgctccatcacgtggtatttgagttggagtccctgcgctgaatgtagccaaaagatcgttgactttcttaaagagcaccccaacgtcaaccttgagatatacgtcgctcgactgtattaccatcataaaggaccttttgtaaaaattgcgagcggcgaaattaggtagttgcgtaacgaggtagtgcaccataaactcaacctcagggacgcgacttacatcggttttctagcaactgaaagaatttctcgtggggttgcagttggaactctatatgcagcgagctgacataatggtaPfoIPmlIBsaAIBsmFIBsrDIPflMIHpy99IBsiHKAIYF46LE48NI50FN52AR54RF56NP58ST60HC62SI64TW66YL68SW70SP72CA74EC76SQ78KI80VD82FL84KE86HP88NV90NL92EI94YV96AR98LY100YH10245-530FERNY180200220240260280300320340
    gaagatgaaaggaatagacaggggctccgagatctcgtgaactctggagtcactatacgcattatggatctgcccgattacaactattgctggaaaactttcgtaagtgaccaaggaggagacgaggattactggccagggcattttgcgccttggataaaacagtatagcttgacttctactttccttatctgtccccgaggctctagagcacttgagacctcagtgatatgcgtaatacctagacgggctaatgttgataacgaccttttgaaagcattcactggttcctcctctgctcctaatgaccggtcccgtaaaacgcggaacctattttgtcatatcgaactMscIMslIStyIStyIXmnIBanIIBcoDIBglIIBseRIBsmAIBsmBIBssSIEsp3IBssSαIED104ER106NR108QG110LR112DL114VN116SG118VT120IR122IM124DL126PD128YN130YC132WK134TF136VS138DQ140GG142DE144DY146WP148GH150FA152PW154IK156QY158SL16045-530FERNY360380400420440460480500520
    agctgaaAtccggtagtgaaacaccggggacttcagaatcggccaccccggAgtgcctgTcatacgaaaccgagatactgacagtagaatatggccttctgccaatcgggaagattgtggagaaacggatagaatgcacagtttactctgtcgataacaatggtaacatttatactcgacttTaggccatcactttgtggcccctgaagtcttagccggtggggccTcacggacAgtatgctttggctctatgactgtcatcttataccggaagacggttagcccttctaacacctctttgcctatcttacgtgtcaaatgagacagctattgttaccattgtaaatatgBsmFIK1KL162S1G2S3E4T5P6G7T8S9E10S11A12T13P14E15534-578CL2SY4ET6EI8LT10VE12YG14LL16PI18GK20IV22EK24RI26EC28TV30YS32VD34NN36GN38IY40T579-887Npu DnaE N-term inteinXTEN linker540560580600620640660680700
    tcagccagttgcccagtggcacgaccggggagagcaggaagtattcgaatactgtctggaggatggaagtctcattagggccactaaggaccacaaatttatgacagtcgatggccagatgctgcctatagacgaaatctttgagcgagagttggacctcatgcgagttgacaacagtcggtcaacgggtcaccgtgctggcccctctcgtccttcataagcttatgacagacctcctaccttcagagtaatcccggtgattcctggtgtttaaatactgtcagctaccggtctacgacggatatctgctttagaaactcgctctcaacctggagtacgctcaactgttgBmrIMscIXcmIAlwNIAvaIIAvaIIBcoDIBsmAIBstBIQ42PV44AQ46WH48DR50GE52QE54VF56EY58CL60ED62GS64LI66RA68TK70DH72KF74MT76VD78GQ80ML82PI84DE86IF88ER90EL92DL94MR96VD98N579-887Npu DnaE N-term intein720740760780800820840860
    cttcctaattaaggcggctgaagagcctggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctgaaggattaattccgccgacttctcggaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcgaatgttaaatccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgaSapISspIBspQIBsrBICspCIEcoNIL100PN102*579-887Npu...in8809009209409609801,0001,0201,040
    catgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcggtacttaattaagaatctttttgagtagctcgtagtttactttgacgttaaataagtatagtcctaatagttatggtataaaaactttttcggcaaagacattacttcctcttttgagtggctccgtcaaggtatcctaccgttctaggaccatagccagacgctaaggctgagcAseIXmnIBceAIBciVIBspHIPflMI*272FF270ED268LM266LH264FQ262LK260NM258DP256ND254IG252YK250QF248LR246KQ244LS242PS240FE238GL236CN234WL232IA230LD228QY226RD224AI222GV220R1064-1879aph (KanR)1,0601,0801,1001,1201,1401,1601,1801,2001,220
    tccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaggttgtagttatgttggataattaaaggggagcagtttttattccaatagttcactctttagtggtactcactgctgacttaggccactcttaccgttttcaaatacgtaaagaaaggtctgaacaagttgtccggtcggtaatgcgagcagtagttttagtgagcgtagttggAseINsiIG218VD216IC214GI212LK210GE208DF206IL204ND202LS200FD198GH196TV194VS192DP190SF188PL186LK184HM182EK180WV178QE176VP174WG172NR170ED168DF166DS164AD162V1064-1879aph (KanR)1,2401,2601,2801,3001,3201,3401,3601,3801,400
    aaaccgttattcattcgtgattgcgcctgagccagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggtttggcaataagtaagcactaacgcggactcggtctgctttatgcgctagcgacaattttcctgttaatgtttgtccttagcttacgttggccgcgtccttgtgacggtcgcgtagttgttataaaagtggacttagtcctataagaagattatggaccttacgacaaaagggccAjuIAvaIBtsIPvuISmaISspIXmaIAsiSIBsoBIBsrFIBtsαIEcoNITspMIBpu10IL160GN158NM156RS154QA152QA150LR148FV146RD144SN142FP140CN138CV136PI134SH132LR130RL128FV126AL124AD122VI120NE118GS116DP114YE112EL110VQ108FA106TK104GP1021064-1879aph (KanR)1,4201,4401,4601,4801,5001,5201,5401,560
    ggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacctagcgtcaccactcattggtacgtagtagtcctcatgcctattttacgaactaccagccttctccgtatttaaggcagtcggtcaaatcagactggtagagtagacattgtagtaaccgttgcgatggaaacggtacaaagtctttgttgagaccgcgtagcccgaagggtatBtsINsiIRsaIBtsαICviQIIA100TT98LL96WA94DD92PT90RI88FH86KI84TP82LP80MF78ET76LW74NL72RV70ME68DT66VD64NA62VS60GK58GH56KL54FL52EP50AD48PK46GY441064-1879aph (KanR)1,5801,6001,6201,6401,6601,6801,7001,7201,740
    caatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttgttagctatctaacagcgtggactaacgggctgtaatagcgctcgggtaaatatgggtatatttagtcgtaggtacaaccttaaattagcgccggatctcgttctgcaaagggcaacttataccgagtattgtggggaacataatgacaaatacattcgtctgtcaaaataacaaClaINruIBanIIBspDILR42YI40TA38GS36QG34VN32DR30AW28KY26GY24LD22AD20MN18SN16LR14PR12SC10ST8ER6QI4HS2M1064-1879aph (KanR)1,7601,7801,8001,8201,8401,8601,8801,9001,920
    catgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactcttgtactggttttagggaattgcactcaaaagcaaggtgactcgcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaHgaIBspHIMspA1IpUC origin high copy1,9401,9601,9802,0002,0202,0402,0602,0802,100
    tttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggaaaggcttccattgaccgaagtcgtctcgcgtctatggtttatgacaagaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcaccgacgacggtcaccgctattcagcacagaatggcccaaccAlwNIBceAIpUC origin high copy2,1202,1402,1602,1802,2002,2202,2402,260
    actcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggtgagttctgctatcaatggcctattccgcgtcgccagcccgacttgccccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccEciIApaLIBaeGIBciVIBseYIHaeIIMspA1IBsiHKAIpUC origin high copy2,2802,3002,3202,3402,3602,3802,4002,4202,440
    cagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcggaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggDrdIEciIHgaIBssSIBssSαIHpy99IpUC origin high copypred...tor2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgagaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctcNspIPciIBsrBIAflIIIpredicte...minator2,6402,6602,6802,7002,7202,7402,760
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    Editing is disabled because sequence edits have been locked.
    2760 bppBT100 backbone200022502500250500C>T45-530579-887

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    2760