Welcome to Benchling!

You're looking at a DNA Sequence. Here are some things you can do with it:

Create an alignment

  1. Create alignments with both DNA and AA sequences.
  2. Bulk-align multiple sequences at once.
  3. Powerful visualization tool to inspect alignments in detail.

Design a primer

Create a translation

Design a CRISPR guide

Assemble and transform a sequence

Create account

Sign up with GoogleI already have an account
Benchling is actively tested against the latest versions of Chrome, Firefox, Safari, and Edge. **It doesn't look like your current browser is supported** - for more information, [click here](/browsers).
Monospace normal
Monospace bold
Advanced Settings
  • 0 feature results
pBZCas13b-HEPN
  • Sequence Map
    Editing disabled on read-only sequence.
    aggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgttccggtacgacaggtccgtccatctactgctggtagtccctgtcgaagttcctagcgagcgccgagaatggtcggattgaagctagtaacctggcgactagcagtgccgctaaatacggcggagccgctcgtgtaccttgcccaaccgtacctaacatccgcggcgggatatggaacaBglIBfuAIBspMIBstAPI20406080100120140160
    ctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactccaagaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaacatatccatcgcgtccgccatctgacggaggggcgcaacgcagcgccacgtacctcggcccggtggagctggacttaccttcggccgccgtggagcgattgcctaagtggtgaggttcttaacctcggttagttaagaacgcctcttgacacttacgcgtttggttgggaaccgtcttgtataggtagcgcaggcggtaga180200220240260280300320340
    ccagcagccgcacgcggcgcatctcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacggtcgtcggcgtgcgccgcgtagagcccgtcgcaacccaggaccggtgcccacgcgtactagcacgaggacagcaactcctgggccgatccgaccgccccaacggaatgaccaatcgtcttacttagtggctatgcgctcgcttgcacttcgctgacgacgacgttttgcagacgctgMscIFspAIPpuMIPpuMI360380400420440460480500520
    ctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcccctacgtgctgctgaagttgcccgcaacagagagtggaaccaaccggtgataccacgatactatgactgagagtcaacgccatgagcggcctcatttcttattctgagttacaagactcgttgttgtacttaccagaagccaaaggcacaaagcatttcagacctttgcgccttcaggggatgcacgacgacttcaacgggcgttgtctctcaccttggttggccactatggtgctatgatactgactctcagttgcggtactcgccggagtaaagaataagactcaatgttAhdIBsaAIHincII540560580600620640660680700
    cagtccgcaccgctgtccggtagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgccctgcaccagtcaggcgtggcgacaggccatcgaggaaggccacccgcgccccgtactgatagcagcggcgtgaatactgacagaagaaatagtacgttgagcatcctgtccacggccgtcgcgggttgtcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgggacgtggt720740760780800820840860880
    ttatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctaaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagaatacaaggcctagacgtagcgtcctacgacgaccgatgggacaccttgtggatgtagacataattgcttcgcgattggcaaaaatagtccgagaccctccgtcttatttactagtatagcagttaataatggaggtgcccctctcggactcgtttgaccggagtccgtaaactcttcBclIBspEIBstYIBsu36I9009209409609801,0001,0201,0401,060
    cacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaatttgctttcgaatttctgccattcatccgcttattatcacttattcaggcgtagcaccaggcgtttaagggcaccaagtgtgccagtgtgacgaaggccatcagttatttggccatttggtcgttatctgtattcgccgataaattgctgggacgggacttggctgctggcccagcttaaacgaaagcttaaagacggtaagtaggcgaataatagtgaataagtccgcatcgtggtccgcaaattcccgtggttBtsIDrdIBaeGIBtsαIPflFITth111I1,0801,1001,1201,1401,1601,1801,2001,2201,240
    taactgccttaaaaaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaattgacggaatttttttaatgcggggcgggacggtgagtagcgtcatgacaacattaagtaattcgtaagacggctgtaccttcggtagtgtctgccgtactacttggacttagcggtcgccgtagtcgtggaacagcggaacgcatattataaacgggtaccacttttgcccccgctScaI1,2601,2801,3001,3201,3401,3601,3801,4001,420
    agaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattctcttcaacaggtataaccggtgcaaatttagttttgaccactttgagtgggtccctaaccgactctgctttttgtataagagttatttgggaaatccctttatccggtccaaaagtggcattgtgcggtgtagaacgcttatatacacatctttgacggcctttagcagcaccataagMscIPasIBsmBIEsp3I1,4401,4601,4801,5001,5201,5401,5601,5801,600
    actccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttcttttgaggtctcgctacttttgcaaagtcaaacgagtaccttttgccacattgttcccacttgtgatagggtatagtggtcgagtggcagaaagtaacggtatgccttaaggcctactcgtaagtagtccgcccgttcttacacttatttccggcctattttgaacacgaataaaaagaaaAclIBspEIEcoRI1,6201,6401,6601,6801,7001,7201,7401,7601,780
    acggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataacttgccagaaatttttccggcattataggtcgacttgccagaccaatatccatgtaactcgttgactgactttacggagttttacaagaaatgctacggtaaccctatatagttgccaccatataggtcactaaaaaaagaggtaaaatcgaaggaatcgaggacttttagagctattgaPvuII1,8001,8201,8401,8601,8801,9001,9201,940
    caaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtatttattcggcgcagttttttatgcgggccatcactagaataaagtaataccactttcaaccttggagaatgcacggctagttgcagagtaaaagcggttttcaaccgggtcccgaagggccatagttgtccctgtggtcctaaataaataagacgcttcactagaaggcagtgtccataaataagccgcgtPasIBsaAIBsmBIEsp3I1,9601,9802,0002,0202,0402,0602,0802,1002,120
    aagtgcgtcgggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcgctagcggagtgtatactggcttactatttcacgcagcccactacgacggttgaatgactaaatcacatactaccacaaaaactccacgaggtcaccgaagacaaagatagtcgacagggaggacaagtcgatgactgccccaccacgcattgccgttttcgtggcggcctgtagtcgcgatcgcctcacatatgaccgaatgataAccIPvuIIBstZ17I2,1402,1602,1802,2002,2202,2402,2602,2802,300
    gttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcaaccgtgactactcccacagtcacttcacgaagtacaccgtcctcttttttccgacgtggccacgcagtcgtcttatacactatgtcctatataaggcgaaggagcgagtgactgagcgatgcgagccagcaagctgacgccgctcgcctttaccgaatgcttgccccgcctctaaaSgrAI2,3202,3402,3602,3802,4002,4202,4402,4602,480
    cctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcggaccttctacggtccttctatgaattgtcccttcactctcccggcgccgtttcggcaaaaaggtatccgaggcggggggactgttcgtagtgctttagactgcgagtttagtcaccaccgctttgggctgtcctgatatttctatggtccgcaaagggggaccgccgagggagcacgPfoIBssSISacIIBssSαI2,5002,5202,5402,5602,5802,6002,6202,6402,660
    gctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtcgagaggacaaggacggaaagccaaatggccacagtaaggcgacaataccggcgcaaacagagtaaggtgcggactgtgagtcaaggcccatccgtcaagcgaggttcgacctgacatacgtgcttggggggcaagtcaggctggcgacgcggaataggccattgatagcagaactca2,6802,7002,7202,7402,7602,7802,8002,8202,840
    ccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgggttgggcctttctgtacgttttcgtggtgaccgtcgtcggtgaccattaactaaatctcctcaatcagaacttcagtacgcggccaattccgatttgactttcctgttcaaaaccactgacgcgaggaggttcggtcaatggagccaagtttctcaaccatcgagtctcttggaagcNspI2,8602,8802,9002,9202,9402,9602,9803,0003,020
    aaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgtaattctctttttggcgggacgttccgccaaaaaagcaaaagtctcgttctctaatgcgcgtctggttttgctagagttcttctagtagaataattagtctattttataaagatctaaagtcacgttaaatagagaagtttacatcgtggacttcagtcggggtatgctatattcaacattaagagAseIXbaI3,0403,0603,0803,1003,1203,1403,1603,1803,200
    atgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgtacaaactgtcgaatagtagctattcgaaattacgccatcaaatagtgtcaatttaacgattgcgtcagtccgtggcacatactttagattgttacgcgagtagcagtaggagccgtggcagtgggacctacgacatccgtatccgaaccaatacggccatgacggcccggagaacgcSfcIGibson hom...oRV-pACYC3,2203,2403,2603,2803,3003,3203,3403,3603,380
    ggatTTTACACTTTATGCTTCCGGCTCGTATGTTAGGAGGTCTTTATCatggaaaacaaaacatctttggggaataatatttattacaacccttttaaaccacaagataaatcgtattttgcgggttatttcaatgcagcaatggaaaatacagatagtgtatttagagaattgggaacctaAAATGTGAAATACGAAGGCCGAGCATACAATCCTCCAGAAATAGtaccttttgttttgtagaaaccccttattataaataatgttgggaaaatttggtgttctatttagcataaaacgcccaataaagttacgtcgttaccttttatgtctatcacataaatctcttaacccttME2NK4TS6LG8NN10IY12YN14PF16KP18QD20KS22YF24AG26YF28NA30AM32EN34TD36SV38FR40EL42G3431-7102BZ large proteinLac promoter3,4003,4203,4403,4603,4803,5003,5203,5403,560
    aaagattaaaaggtaaagagtacacttcggagaatttttttgatgctatctttaaggaaaatatttcattagtggaatacgaaaggtatgtgaaattgctttctgattatttccctatggctcgtcttttggataaaaaagaagttcctataaaagaacgaaaagaaaattttaaaaatttctaattttccatttctcatgtgaagcctcttaaaaaaactacgatagaaattccttttataaagtaatcaccttatgctttccatacactttaacgaaagactaataaagggataccgagcagaaaacctattttttcttcaaggatattttcttgcttttcttttaaaatttttK44RL46KG48KE50YT52SE54NF56FD58AI60FK62EN64IS66LV68EY70ER72YV74KL76LS78DY80FP82MA84RL86LD88KK90EV92PI94KE96RK98EN100FK102K3431-7102BZ large protein3,5803,6003,6203,6403,6603,6803,7003,720
    gaattttaaaggcattataaaagccgtaagagatttaGCAaacttttatacgGCAaaggaacacggagaggtagaaattacagatgaaatatttggcgtattggatgagatgctgaaaagcacggttttaacggtaaaaaagaaaaaagtaaaaaccgataaaacaaaagaaatcctgcttaaaatttccgtaatattttcggcattctctaaatCGTttgaaaatatgcCGTttccttgtgcctctccatctttaatgtctactttataaaccgcataacctactctacgacttttcgtgccaaaattgccattttttcttttttcatttttggctattttgttttctttaggacN104FK106GI108IK110AV112RD114LA116NF118YT120AK122EH124GE126VE128IT130DE132IF134GV136LD138EM140LK142ST144VL146TV148KK150KK152VK154TD156KT158KE160IL1623431-7102BZ large proteinHEPN13,7403,7603,7803,8003,8203,8403,8603,8803,900
    aaaaaaagcatagaaaaacaattggatattttatgccaaaagaaattagaatatctaagagacacagcaagaaaaatagaggagaagcgtagaaatcaaagagaaagaggagagaaagaattggtagctccattcaaatacagtgataaaagggatgatttaaTAGCAGCGATTTATAtttttttcgtatctttttgttaacctataaaatacggttttctttaatcttatagattctctgtgtcgttctttttatctcctcttcgcatctttagtttctctttctcctctctttcttaaccatcgaggtaagtttatgtcactattttccctactaaattATCGTCGCTAAATATMfeIKK164SI166EK168QL170DI172LC174QK176KL178EY180LR182DT184AR186KI188EE190KR192RN194QR196ER198GE200KE202LV204AP206FK208YS210DK212RD214DL216IA218AI220Y3431-7102BZ large protein3,9203,9403,9603,9804,0004,0204,0404,0604,080
    ATGATGCTTTTGATGtttatatcgataagaaaaaagatagcctaaaagaatccagcaaagcaaagtacaataccaaaagtgatcctcagcaagaagaaggcgatttgaaaattccaatttcgaaaaacggagtagtcttcctgctttctttgtttctcaccaaacaagaaatacacgcTACTACGAAAACTACaaatatagctattcttttttctatcggattttcttaggtcgtttcgtttcatgttatggttttcactaggagtcgttcttcttccgctaaacttttaaggttaaagctttttgcctcatcagaaggacgaaagaaacaaagagtggtttgttctttatgtgcgBbvCIN222DA224FD226VY228ID230KK232KD234SL236KE238SS240KA242KY244NT246KS248DP250QQ252EE254GD256LK258IP260IS262KN264GV266VF268LL270SL272FL274TK276QE278IH280A3431-7102BZ large protein4,1004,1204,1404,1604,1804,2004,2204,2404,260
    attcaaatctaaaatagcagggtttaaggctacggttatcgatgaagcaacggtatctgaagcaacggtgagccatggaaaaaacagcatttgttttatggctacgcacgaaattttctcgcatttggcttacaaaaaattgaaacgaaaagtgagaacggctgaaatcaactatggttaagtttagattttatcgtcccaaattccgatgccaatagctacttcgttgccatagacttcgttgccactcggtaccttttttgtcgtaaacaaaataccgatgcgtgctttaaaagagcgtaaaccgaatgttttttaactttgcttttcactcttgccgactttagttgataccaF282KS284KI286AG288FK290AT292VI294DE296AT298VS300EA302TV304SH306GK308NS310IC312FM314AT316HE318IF320SH322LA324YK326KL328KR330KV332RT334AE336IN338YG3403431-7102BZ large protein4,2804,3004,3204,3404,3604,3804,4004,4204,440
    gaggcagaaaatgctgaacagctttccgtttatgccaaagaaactctaatgatgcagatgcttgatgaattgagcaaagtacccgatgtggtctatcagaatttgagcgaagatgtacaaaaaactttcatcgaagattggaatgaatacctaaaagagaacaatggcgatgtagggactccgtcttttacgacttgtcgaaaggcaaatacggtttctttgagattactacgtctacgaactacttaactcgtttcatgggctacaccagatagtcttaaactcgcttctacatgttttttgaaagtagcttctaaccttacttatggattttctcttgttaccgctacatccctBsrGIBstAPIEA342EN344AE346QL348SV350YA352KE354TL356MM358QM360LD362EL364SK366VP368DV370VY372QN374LS376ED378VQ380KT382FI384ED386WN388EY390LK392EN394NG396DV398G3431-7102BZ large protein4,4604,4804,5004,5204,5404,5604,5804,6004,620
    caatggaagaagagcaggtaattcatccagtgattcgcaagcgttacgaagataaattcaattattttgccattcgatttttagatgagtttgctcagttccctacgcttcgttttcaagtgcatttagggaattatcttcacgattctcgccctaaagaaaatctgatttctgaccggttaccttcttctcgtccattaagtaggtcactaagcgttcgcaatgcttctatttaagttaataaaacggtaagctaaaaatctactcaaacgagtcaagggatgcgaagcaaaagttcacgtaaatcccttaatagaagtgctaagagcgggatttcttttagactaaagactggcSapIBfuAIBspMIBspQIT400ME402EE404QV406IH408PV410IR412KR414YE416DK418FN420YF422AI424RF426LD428EF430AQ432FP434TL436RF438QV440HL442GN444YL446HD448SR450PK452EN454LI456SD458R3431-7102BZ large protein4,6404,6604,6804,7004,7204,7404,7604,7804,800
    aagaataaaagaaaaaatcaccgttttcggaaggctttcggaattggaacataaaaaagcattgtttataaaaaataccgaaaccaatgaagatagagaacactattgggaaatttttcctaaccccaattatgattttcctaaggaaaatatttccgttaatgataaagattttcctttcttattttcttttttagtggcaaaagccttccgaaagccttaaccttgtattttttcgtaacaaatattttttatggctttggttacttctatctcttgtgataaccctttaaaaaggattggggttaatactaaaaggattccttttataaaggcaattactatttctaaaaggaBsu36IR460IK462EK464IT466VF468GR470LS472EL474EH476KK478AL480FI482KN484TE486TN488ED490RE492HY494WE496IF498PN500PN502YD504FP506KE508NI510SV512ND514KD516FP5183431-7102BZ large protein4,8204,8404,8604,8804,9004,9204,9404,9604,980
    atcgcgggtagtattttggacagagagaaacagcctgtcgcagggaaaattggtataaaagtgaaattgcttaaccaacaatatgtttcagaagtagataaagccgtaaaagcccatcagctcaagcaaagaaaagccagcaagccgtctatccagaacattatagaagaaatagtgctagcgcccatcataaaacctgtctctctttgtcggacagcgtcccttttaaccatattttcactttaacgaattggttgttatacaaagtcttcatctatttcggcattttcgggtagtcgagttcgtttcttttcggtcgttcggcagataggtcttgtaatatcttctttatcacgIA520GS522IL524DR526EK528QP530VA532GK534IG536IK538VK540LL542NQ544QY546VS548EV550DK552AV554KA556HQ558LK560QR562KA564SK566PS568IQ570NI572IE574EI576V3431-7102BZ large protein5,0005,0205,0405,0605,0805,1005,1205,1405,160
    caatcaatgaaagcaatccaaaagaagctattgttttcggtgggcaacccacggcttatctgagtatgaatgacattcattccattttgtatgagtttttcgataaatgggagaaaaagaaagaaaagttagaaaagaaaggtgaaaaagaattaagaaaggaaattggaaaagagttgttagttactttcgttaggttttcttcgataacaaaagccacccgttgggtgccgaatagactcatacttactgtaagtaaggtaaaacatactcaaaaagctatttaccctctttttctttcttttcaatcttttctttccactttttcttaattctttcctttaaccttttctcaaP578IN580ES582NP584KE586AI588VF590GG592QP594TA596YL598SM600ND602IH604SI606LY608EF610FD612KW614EK616KK618EK620LE622KK624GE626KE628LR630KE632IG634KE636L3431-7102BZ large protein5,1805,2005,2205,2405,2605,2805,3005,3205,340
    agaaaagaaaatcgtaggaaaaattcaagctcaaatccagcaaatcatagataaagataccaatgccaagatattaaaaccttatcaagacggaaattctactgctattgataaagagaaattgataaaagatttgaagcaagagcaaaacattctacaaaaattaaaagatgaacaatcttttcttttagcatcctttttaagttcgagtttaggtcgtttagtatctatttctatggttacggttctataattttggaatagttctgcctttaagatgacgataactatttctctttaactattttctaaacttcgttctcgttttgtaagatgtttttaattttctacttgttE638KK640IV642GK644IQ646AQ648IQ650QI652ID654KD656TN658AK660IL662KP664YQ666DG668NS670TA672ID674KE676KL678IK680DL682KQ684EQ686NI688LQ690KL692KD694EQ6963431-7102BZ large protein5,3605,3805,4005,4205,4405,4605,4805,500
    acggttcgtgaaaaagaatataatgattttattgcttatcaagataaaaatagggagataaataaagtcagagatagaaatcataaacaatatttaaaagataaccttaaacgaaagtaccctgaagctcctgcacgaaaagaagttctttattatcgagaaaaaggaaaagtagcggtgccaagcactttttcttatattactaaaataacgaatagttctatttttatccctctatttatttcagtctctatctttagtatttgttataaattttctattggaatttgctttcatgggacttcgaggacgtgcttttcttcaagaaataatagctctttttccttttcatcgccBaeITV698RE700KE702YN704DF706IA708YQ710DK712NR714EI716NK718VR720DR722NH724KQ726YL728KD730NL732KR734KY736PE738AP740AR742KE744VL746YY748RE750KG752KV754A3431-7102BZ large protein5,5205,5405,5605,5805,6005,6205,6405,6605,680
    tttggttggcgaatgacatcaaacgctttatgcctaccgatttcaaaaacgaatggaaaggggagcaacacagcttgttacaaaaatcgttggcgtattatgagcaatgtaaagaggagttgaaaaatcttttgcctgaaaaagtatttcagcatttaccttttaagttgggaggataaaaccaaccgcttactgtagtttgcgaaatacggatggctaaagtttttgcttacctttcccctcgttgtgtcgaacaatgtttttagcaaccgcataatactcgttacatttctcctcaactttttagaaaacggactttttcataaagtcgtaaatggaaaattcaaccctcctatV756WL758AN760DI762KR764FM766PT768DF770KN772EW774KG776EQ778HS780LL782QK784SL786AY788YE790QC792KE794EL796KN798LL800PE802KV804FQ806HL808PF810KL812GG814Y3431-7102BZ large protein5,7005,7205,7405,7605,7805,8005,8205,8405,860
    ttttcaacaaaaatatttgtaccaattttacacttgttatttagataaacggttggaatacatcagtgggttggtgcaacaagccgaaaactttaaatccgaaaataaagtcttcaaaaaggtagaaaatgagtgttttaaatttttgaaaaaacaaaattatactcacaaagaattaaaaagttgtttttataaacatggttaaaatgtgaacaataaatctatttgccaaccttatgtagtcacccaaccacgttgttcggcttttgaaatttaggcttttatttcagaagtttttccatcttttactcacaaaatttaaaaacttttttgttttaatatgagtgtttcttaatF816QQ818KY820LY822QF824YT826CY828LD830KR832LE834YI836SG838LV840QQ842AE844NF846KS848EN850KV852FK854KV856EN858EC860FK862FL864KK866QN868YT870HK872EL8743431-7102BZ large protein5,8805,9005,9205,9405,9605,9806,0006,0206,040
    gatgctcgggttcagtctatattggggtatcctatctttttagagcggggctttatggatgaaaaacctacgataatcaaaggaaaaacttttaaaggaaatgaagctctttttgccgattggtttaggtattacaaggaatatcagaattttcagacattttatgatactgaaaattctacgagcccaagtcagatataaccccataggatagaaaaatctcgccccgaaatacctactttttggatgctattagtttcctttttgaaaatttcctttacttcgagaaaaacggctaaccaaatccataatgttccttatagtcttaaaagtctgtaaaatactatgacttttaaBciVIDA876RV878QS880IL882GY884PI886FL888ER890GF892MD894EK896PT898II900KG902KT904FK906GN908EA910LF912AD914WF916RY918YK920EY922QN924FQ926TF928YD930TE932N3431-7102BZ large protein6,0606,0806,1006,1206,1406,1606,1806,2006,220
    atccgcttgtagaattggaaaaaaaacaagcagaccgaaaacgaaaaactaaaatctaccaacaaaagaaaaacgatgtgtttacacttttgatggcaaaacatattttcaaatcggtattcaagcaggatagcatcgatcagttttcgttagaagatttgtatcaatctcgggaggataggcgaacatcttaaccttttttttgttcgtctggcttttgctttttgattttagatggttgttttctttttgctacacaaatgtgaaaactaccgttttgtataaaagtttagccataagttcgtcctatcgtagctagtcaaaagcaatcttctaaacatagttagagccctcctY934PL936VE938LE940KK942QA944DR946KR948KT950KI952YQ954QK956KN958DV960FT962LL964MA966KH968IF970KS972VF974KQ976DS978ID980QF982SL984ED986LY988QS990RE992E3431-7102BZ large protein6,2406,2606,2806,3006,3206,3406,3606,3806,400
    acgcttaggaaatcaggagagagccagacaaacgggcgagcgaaatactaactatatttggaacaaaaccgttgatttaaagttgtgtgacggaaaaattacggtagaaaatgtaaagctaaaaaatgtaggagatttcataaaatatgaatacgaccaaagggttcaagctttcttatgcgaatcctttagtcctctctcggtctgtttgcccgctcgctttatgattgatataaaccttgttttggcaactaaatttcaacacactgcctttttaatgccatcttttacatttcgattttttacatcctctaaagtattttatacttatgctggtttcccaagttcgaaagaatR994LG996NQ998ER1000AR1002QT1004GE1006RN1008TN1010YI1012WN1014KT1016VD1018LK1020LC1022DG1024KI1026TV1028EN1030VK1032LK1034NV1036GD1038FI1040KY1042EY1044DQ1046RV1048QA1050FL10523431-7102BZ large protein6,4206,4406,4606,4806,5006,5206,5406,5606,580
    aaatatgaggaaaatatagaatggcaggcgtttctaatcaaagagagtaaggaagaggaaaattatccgtatgtggtggaaagagaaatagagcaatatgaaaaagtgagaagggaagaactgttgaaggaagtccaccttatagaggaatatattttggaaaaggtaaaagataaggtttatactccttttatatcttaccgtccgcaaagattagtttctctcattccttctccttttaataggcatacaccacctttctctttatctcgttatactttttcactcttcccttcttgacaacttccttcaggtggaatatctccttatataaaaccttttccattttctattccKY1054EE1056NI1058EW1060QA1062FL1064IK1066ES1068KE1070EE1072NY1074PY1076VV1078ER1080EI1082EQ1084YE1086KV1088RR1090EE1092LL1094KE1096VH1098LI1100EE1102YI1104LE1106KV1108KD1110K3431-7102BZ large protein6,6006,6206,6406,6606,6806,7006,7206,7406,760
    aaatattaaaaaaaggggataatcaaaatttcaaatactacattctcaacggacttttaaagcaacttaaaaatgaagatgtggagagctataaggtattcaacttaaatactgaacctgaagatgtgaacattaaccaactaaaacaagaagcaaccgatttagaacaaaaagcttttttataatttttttcccctattagttttaaagtttatgatgtaagagttgcctgaaaatttcgttgaatttttacttctacacctctcgatattccataagttgaatttatgacttggacttctacacttgtaattggttgattttgttcttcgttggctaaatcttgtttttcgaaaE1112IL1114KK1116GD1118NQ1120NF1122KY1124YI1126LN1128GL1130LK1132QL1134KN1136ED1138VE1140SY1142KV1144FN1146LN1148TE1150PE1152DV1154NI1156NQ1158LK1160QE1162AT1164DL1166EQ1168KA1170F3431-7102BZ large protein6,7806,8006,8206,8406,8606,8806,9006,9206,940
    tgttttaacctatattGCAaataaatttgcaGCAaaccaattacctaaaaaagaattttgggactattgtcaagaaaaatacggcaaaatagaaaaagaaaaaacctatgccgagtattttgctgaggtatttaaaaaggaaaaagaagcactgataaagTAATTGACAGCTAGCTCAacaaaattggatataaCGTttatttaaacgtCGTttggttaatggattttttcttaaaaccctgataacagttctttttatgccgttttatctttttcttttttggatacggctcataaaacgactccataaatttttcctttttcttcgtgactatttcATTAACTGTCGATCGAGTBbvCIV1172LT1174YI1176AN1178KF1180AA1182NQ1184LP1186KK1188EF1190WD1192YC1194QE1196KY1198GK1200IE1202KE1204KT1206YA1208EY1210FA1212EV1214FK1216KE1218KE1220AL1222IK12243431-7102*1BZ large proteinHEPN2J231...ter6,9606,9807,0007,0207,0407,0607,0807,1007,120
    GTCCTAGGTATAATGCTAGCGCTGAGTTGGAACTGCTCTCATTTTGGAGGGTAATCACAACGAGACCATGGCGGAAGGTCTCAGTTGGAACTGCTCTCATTTTGGAGGGTAATCACAACatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttCAGGATCCATATTACGATCGCGACTCAACCTTGACGAGAGTAAAACCTCCCATTAGTGTTGCTCTGGTACCGCCTTCCAGAGTCAACCTTGACGAGAGTAAAACCTCCCATTAGTGTTGtagcaggtaaggctgtcgtagcggtcagtgataccgcacgacgatcgcgatatacgcaaBsaIBsaIAvrIIBZ DRBZ DRGibson homol...EcoRV pACYCJ23119 promoter7,1407,1607,1807,2007,2207,2407,2607,280
    gatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtctacgttaaagatacgcgtgggcaagagcctcgtgacaggctggcgaaaccggcggcgggtcaggacgagcgaagcgatgaacctcggtgatagctgatgcgctagtaccgctggtgtgggcaggacacctaggagatgcggcctgcgtagcaccggccgtagtggccgcggtgtccaBamHIBstYIFspAISgrAI7,3007,3207,3407,3607,3807,4007,4207,4407,460
    gcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcacgccaacgaccgcggatatagcggctgtagtggctaccccttctagcccgagcggtgaagcccgagtactcgcgaacaaagccgcacccataccaccgtccggggcaccggccccctgacaacccgcggtagaggaacgtacgtggtaaggaacgccgccgccacgagttgccggagtNspISphIBanIIBanIIBspHI7,4807,5007,5207,5407,5607,5807,6007,6207,640
    acctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgcttggatgatgacccgacgaaggattacgtcctcagcgtattccctctcgcagctggctacgggaactctcggaagttgggtcagtcgaggaaggccacccgcgccccgtactgatagcagcggcgtgaatactgacagaagaaatagtacgttgagcatcctgtccacggccgtcgcgaAccISalIHincII7,6607,6807,7007,7207,7407,7607,7807,8007,820
    ctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgacccagtaaaagccgctcctggcgaaagcgacctcgcgctgctactagccggacagcgaacgccataagccttagaacgtgcgggagcgagttcggaagcagtgaccagggcggtggtttgcaaagccgctcttcgtccggtaatagcggccgtaccgccggctgcgcgacccgatgAclIBglIEagIBseYI7,8407,8607,8807,9007,9207,9407,9607,9808,000
    gtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgccagaacgaccgcaagcgctgcgctccgacctaccggaaggggtaatactaagaagagcgaaggccgccgtagccctacgggcgcaacgNruI8,0208,0408,0608,080
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    8098 bp6000650070007500500100015002000BZ large proteinHEPN23431-71027103-7105BstAPI,BfuAI,BspMI,BglI,PpuMI,MscI,FspAI,PpuMIBsaAI,AhdI,HincIIBspEI,BstYIBclI,Bsu36I,BtsI,BtsαIDrdI,PflFI,Tth111I,BaeGI,ScaIMscIPasI,BsmBI,Esp3IAclI,EcoRI,BspEIPvuIIBsaAIBciVIJ23119 promoter,BbvCI,AvrIIBZ DR,BsaI,spacer,BsaI,BZ DR,Gibson homology R EcoRV pACYC,BsaI,BsaI,FspAIBamHI,BstYI,SgrAI,BanII,BanII,BspHI,NspI,SphISalI,AccI,HincII,AclI,BglI,EagI,BseYI,NruI

    Welcome to Benchling!

    Sign in to view data.

    New digest

    Saved digests

    Enzyme AatII
    Enzyme lists
    Cut sites visible on maps
    Find enzyme
    Clear selected
    Name Cuts
    Selected Color
    Show enzymes that cut

    Primers

    Pairs

    Create primers
    Attach existing
    Detach all primers
    Primer Position Product size

    Version history

     Show full history
    Change User Timestamp

    Sequence Alignments

    Create new alignment
    Saved alignments

    Sequence Info

    Tags

    Name
    Parent version
    Folder
    Topology
    Start index
    Locked
    Created
    --
    Length
    --
    Export data
    Download
    Split Workspace
    Bases
    8098