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EW368 CMV-TO-UtroUpZF-deImmunLink-NZF(FLP-IN)
  • Sequence Map
    Editing disabled on read-only sequence.
    cggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccgccatttaccgggcggaccgactggcgggttgctgggggcgggtaactgcagttattactgcatacaagggtatcattgcggttatccctgaaaggtaactgcagttacccacctcataaatgccatttgacgggtgaaccgtcatgtagttcacatagtatacggttcatgcggCMVCMV promoter (with TetOs)20406080100120140160
    ccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttgggataactgcagttactgccatttaccgggcggaccgtaatacgggtcatgtactggaataccctgaaaggatgaaccgtcatgtagatgcataatcagtagcgataatggtaccactacgccaaaaccgtcatgtagttacccgcacctatcgccaaactgagtgcccctaaaNcoICspCISnaBICMVCMV promoter (with TetOs)180200220240260280300320340
    ccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcaggttcagaggtggggtaactgcagttaccctcaaacaaaaccgtggttttagttgccctgaaaggttttacagcattgttgaggcggggtaactgcgtttacccgccatccgcacatgccaccctccagatatattcgtctcgagagggatagtcactatctctagagggatagtSacIBsaBIEco53kITetR binding sitesTetOTetOCMVCMV promoter (with TetOs)360380400420440460480500520
    gtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccggactctagcgtttaaacttaagcttcgccaccatgATATTAGACCGGCCCTATGCGTGCCCAGTCGcactatctctagcagctgctcgagcaaatcacttggcagtctagcggacctctgcggtaggtgcgacaaaactggaggtatcttctgtggccctggctaggtcggaggcctgagatcgcaaatttgaattcgaagcggtggtacTATAATCTGGCCGGGATACGCACGGGTCAGCPmeISacIAflIIBsaBISgrDIEco53kIHindIIIIL2DR4PY6AC8PV10670-1206M1UtroUpTe...sTetOCMV promoter (with TetOs)CMV540560580600620640660680700
    AATCTTGCGATCGTCGCTTCAGCAGAAGTGATAACTTAGTCAGACACATAAGGATCCACACGGGTCAAAAACCGTTCCAATGCAGGATTTGTATGAGGAATTTCTCGCGGAGTGACCACCTCACGACACACAACCGCACTCACACCGGAGAGAAACCATTTGCATGCGATATATGTTAGAACGCTAGCAGCGAAGTCGTCTTCACTATTGAATCAGTCTGTGTATTCCTAGGTGTGCCCAGTTTTTGGCAAGGTTACGTCCTAAACATACTCCTTAAAGAGCGCCTCACTGGTGGAGTGCTGTGTGTTGGCGTGAGTGTGGCCTCTCTTTGGTAAACGTACGCTATATACBamHIES12CD14RR16FS18RS20DN22LV24RH26IR28IH30TG32QK34PF36QC38RI40CM42RN44FS46RS48DH50LT52TH54NR56TH58TG60EK62PF64AC66DI68C670-1206UtroUp720740760780800820840860
    CGGCAGGAAGTTCGCTGATCCGGGACATCTAGTCCGTCACAATCGGATACACACCGGGGAAAAGCCGTTTGCGTGCCCTGTGGAAAGCTGCGATCGAAGATTCTCGCGCTCCGACGAACTAACTCGCCATATTAGAATACATACCGGACAGAAGCCCTTTCAGTGTCGGATCTGCGCCGTCCTTCAAGCGACTAGGCCCTGTAGATCAGGCAGTGTTAGCCTATGTGTGGCCCCTTTTCGGCAAACGCACGGGACACCTTTCGACGCTAGCTTCTAAGAGCGCGAGGCTGCTTGATTGAGCGGTATAATCTTATGTATGGCCTGTCTTCGGGAAAGTCACAGCCTAGACGAhdIBcgIPfoIG70RK72FA74DP76GH78LV80RH82NR84IH86TG88EK90PF92AC94PV96ES98CD100RR102FS104RS106DE108LT110RH112IR114IH116TG118QK120PF122QC124RI126C670-1206UtroUp8809009209409609801,0001,0201,040
    ATGCGTAACTTTTCGTCAAGGGACGTACTGAGAAGACATAACAGGACACATACCGGGGAAAAGCCATTTGCGTGCGACATATGTGGGAGAAAGTTCGCTTCCCGGGATGTACTTAGGAGGCATAATCGGATTCATCTACGCCAAAATGACTTGGAAgctgctgcaTGGGAGCTAgTACGCATTGAAAAGCAGTTCCCTGCATGACTCTTCTGTATTGTCCTGTGTATGGCCCCTTTTCGGTAAACGCACGCTGTATACACCCTCTTTCAAGCGAAGGGCCCTACATGAATCCTCCGTATTAGCCTAAGTAGATGCGGTTTTACTGAACCTTcgacgacgtACCCTCGATcSmaIXmaITspMIM128RN130FS132SR134DV136LR138RH140NR142TH144TG146EK148PF150AC152DI154CG156RK158FA160SR162DV164LR166RH168NR170IH172LR174QN176DL178E670-1206A1A2A3W4E5L61207-1242UtroUp1,0601,0801,1001,1201,1401,1601,1801,2001,220
    gaATGtctggcggatctGAGGGCCAGAGCGACGAGAGAGCCCTGCTGGACCAGCTGCACACCCTGCTGAGCAACACCGACGCCACCGGCCTGGAGGAGATCGACAGAGCCCTGGGCATCCCCGAGCTGGTGAACCAGGGCCAGGCCCTGGAGCCCAAGCAGGACGGAGGCTGGGGctTACagaccgcctagaCTCCCGGTCTCGCTGCTCTCTCGGGACGACCTGGTCGACGTGTGGGACGACTCGTTGTGGCTGCGGTGGCCGGACCTCCTCTAGCTGTCTCGGGACCCGTAGGGGCTCGACCACTTGGTCCCGGTCCGGGACCTCGGGTTCGTCCTGCCTCCGACCCCBlpIBsgIPasIBstXIPflMIEG2QS4DE6RA8LL10DQ12LH14TL16LS18NT20DA22TG24LE26EI28DR30AL32GI34PE36LV38NQ40GQ42AL44EP46KQ48DG50GW52G1243-1698G7M8S9G10G11S121207-1242NZF DomainN (NCOA3) Activation Domain1,2401,2601,2801,3001,3201,3401,3601,3801,400
    ATCTATGGCCGAGGAATTCGTGACCCTGAAGGACGTGGGCATGGACTTCACCCTGGGCGACTGGGAGCAGCTGGGCCTGGAGCAGGGCGACACCTTCTGGGACACCGCCCTGGACAACTGCCAGGACCTGTTCCTGCTGGACCCCCCCGGCGGAGGAGACGGCCTTGGTTTGGGATAGATACCGGCTCCTTAAGCACTGGGACTTCCTGCACCCGTACCTGAAGTGGGACCCGCTGACCCTCGTCGACCCGGACCTCGTCCCGCTGTGGAAGACCCTGTGGCGGGACCTGTTGACGGTCCTGGACAAGGACGACCTGGGGGGGCCGCCTCCTCTGCCGGAACCAAACCCTPasIBmgBIEcoNIEcoRIPpuMIS54MA56EE58FV60TL62KD64VG66MD68FT70LG72DW74EQ76LG78LE80QG82DT84FW86DT88AL90DN92CQ94DL96FL98LD100PP102GG104GD106GL108GL110G1243-1698NZF DomainZ (ZNF473) Activation Domain1,4201,4401,4601,4801,5001,5201,5401,560
    CACGAGAAGTTCCCCAGCGACCTGGACCTGGACATGTTCAACGGCAGCCTGGAGTGCGACATGGAGAGCATCATCAGAAGCGAGCTGATGGACGCCGACGGCCTGGACTTCAACTTCGACAGCtaggccgctcgaggagggcccgaacaaaaactcatctcagaagaggatctgaGTGCTCTTCAAGGGGTCGCTGGACCTGGACCTGTACAAGTTGCCGTCGGACCTCACGCTGTACCTCTCGTAGTAGTCTTCGCTCGACTACCTGCGGCTGCCGGACCTGAAGTTGAAGCTGTCGatccggcgagctcctcccgggcttgtttttgagtagagtcttctcctagactApaIPciITliIXhoIBstXIPspXIPaeR7IPspOMI*1H112EK114FP116SD118LD120LD122MF124NG126SL128EC130DM132ES134II136RS138EL140MD142AD144GL146DF148NF150DS1521243-1698NZF DomainF (FOXO3) Activation Domainc-myc epitope1,5801,6001,6201,6401,6601,6801,7001,7201,740
    atagcgccgtcgaccatcatcataatcatcattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcatatcgcggcagctggtagtagtattagtagtaactcaaatttgggcgactagtcggagctgacacggaagatcaacggtcggtagacaacaaacggggagggggcacggaaggaactgggaccttccacggtgagggtgacaggaaaggattattttactcctttaacgtagcgtBclIPmeIBGH polyadenylation sequencePolyhi...e tag1,7601,7801,8001,8201,8401,8601,8801,9001,920
    ttgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggagatctatcgatgttctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggaacagactcatccacagtaagataagaccccccaccccaccccgtcctgtcgttccccctcctaacccttctgttatcgtccgtacgacccctacgccacccgagatacctctagatagctacaagacccctacgccacccgagataccgaagactccgcctttcttggtcgaccClaIBspDIBGH polyadenylation sequenceBGH pA1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggccgagatcccccataggggtgcgcgggacatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccNaeINgoMIV2,1202,1402,1602,1802,2002,2202,2402,260
    ggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagttccgagggaaatcccaaggctaaatcacgaaatgccgtggagctggggttttttgaactaatcccactaccaagtgcatggatcttcaaggataaggcttcaaggataagagatctttcatatccttgaaggaaccggtttttcggacttgagtggcgctgcagacagctcttcaaBarIMscIXbaIPshAIFRTHygromycin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    tctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcagactagcttttcaagctgtcgcagaggctggactacgtcgagagcctcccgcttcttagagcacgaaagtcgaagctacatcctcccgcacctatacaggacgcccatttatcgacgcggctaccaaagatgtttctagcaatacaaatagccgtgaaacgtagccggcgcgagBsaXIPflFITth111IHygromycin2,4602,4802,5002,5202,5402,5602,5802,6002,620
    ccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgaggctaaggccttcacgaactgtaaccccttaagtcgctctcggactggataacgtagagggcggcacgtgtcccacagtgcaacgttctggacggactttggcttgacgggcgacaagacgtcggccagcgcctccggtacctacgctagcgacgccggctagaatcggtctgctNcoIPstIAsiSIBfuAIBspMIEcoRIDraIIIHygromycin2,6402,6602,6802,7002,7202,7402,7602,7802,800
    gcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgcccaagccgggtaagcctggcgttccttagccagttatgtgatgtaccgcactaaagtatacgcgctaacgactaggggtacacatagtgaccgtttgacactacctgctgtggcagtcacgcaggcagcgcgtccgagagctactcgactacgaaacccggctcctgacgggPflFIRsrIITth111IHygromycin2,8202,8402,8602,8802,9002,9202,9402,960
    cgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgcgcttcaggccgtggagcacgtgcgcctaaagccgaggttgttacaggactgcctgttaccggcgtattgtcgccagtaactgacctcgctccgctacaagcccctaagggttatgctccagcggttgtagaagaagacctccggcaccaaccgaacatacctcgtcgtctgcgcgAjuIDraIIIHygromycin2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcatgaagctcgcctccgtaggcctcgaacgtcctagcggcgccgaggcccgcatatacgaggcgtaaccagaactggttgagatagtctcgaaccaactgccgttaaagctactacgtcgaacccgcgtcccagctacgctgcgttagcaggctaggcctcggccctgacagcccgSacIIHygromycin3,1603,1803,2003,2203,2403,2603,2803,3003,320
    gtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttcatgtgtttagcgggcgtcttcgcgccggcagacctggctaccgacacatcttcatgagcggctatcacctttggctgcggggtcgtgagcaggctcccgtttccttatcgtgcatgatgctctaaagctaaggtggcggcggaagatactttccaacccgaagccttagcaaaaScaIHygromycin3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcggccctgcggccgacctactaggaggtcgcgcccctagagtacgacctcaagaagcgggtggggttgaacaaataacgtcgaatattaccaatgtttatttcgttatcgtagtgtttaaagtgtttatttcgtaaaaaaagtgacgtaagatcaacaccaaacaggtttgagtagBsmINaeIPfoIPsiINgoMIVSV40 pA3,5203,5403,5603,5803,6003,6203,6403,660
    aatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattacatagaatagtacagacatatggcagctggagatcgatctcgaaccgcattagtaccagtatcgacaaaggacacactttaacaataggcgagtgttaaggtgtgttgtatgctcggccttcgtatttcacatttcggaccccacggattactcactcgattgagtgtaattBstZ17ISV40 pA3,6803,7003,7203,7403,7603,7803,8003,8203,840
    ttgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagccggttgcgcgcccctctccgccaaacgcataacccgcgagaaggcgaaggagcgagtgactgagcgacgcgagccagcaagccgacgccgctcgccatagtcgagtgagSapIBsaXIBspQI3,8603,8803,9003,9203,9403,9603,9804,0004,020
    aaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgatttccgccattatgccaataggtgtcttagtcccctattgcgtcctttcttgtacactcgttttccggtcgttttccggtccttggcatttttccggcgcaacgaccgcaaaaaggtatccgaggcggggggactgctcgtagtgtttttagctgcgagttcagtctccaccgctPciIpUC ori4,0404,0604,0804,1004,1204,1404,1604,1804,200
    aacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctttgggctgtcctgatatttctatggtccgcaaagggggaccttcgagggagcacgcgagaggacaaggctgggacggcgaatggcctatggacaggcggaaagagggaagcccttcgcaccgcgaaagagtatcgagtgcgacatccatagagtcaagccacatccagcaagcgapUC ori4,2204,2404,2604,2804,3004,3204,3404,360
    ccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtgggttcgacccgacacacgtgcttggggggcaagtcgggctggcgacgcggaataggccattgatagcagaactcaggttgggccattctgtgctgaatagcggtgaccgtcgtcggtgaccattgtcctaatcgtctcgctccatacatccgccacgatgtctcaagaacttcacpUC ori4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaacaccggattgatgccgatgtgatcttcttgtcataaaccatagacgcgagacgacttcggtcaatggaagcctttttctcaaccatcgagaactaggccgtttgtttggtggcgaccatcgccaccaaaaaaacaaacgttcgtcgtctaatgcgcgtctttttttcctagagttpUC ori4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacccttctaggaaactagaaaagatgccccagactgcgagtcaccttgcttttgagtgcaattccctaaaaccagtactctaatagtttttcctagaagtggatctaggaaaatttaatttttacttcaaaatttagttagatttcatatatactcatttgaaccagactgtcaatggBspHIpUC ori4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaattacgaattagtcactccgtggatagagtcgctagacagataaagcaagtaggtatcaacggactgaggggcagcacatctattgatgctatgccctcccgaatggtagaccggggtcacgacgttactatggcgctctgggtgcgagtggccgaggtctaaatagtcgttatttAhdIBsaIBsrDIAmp (bla)4,9204,9404,9604,9805,0005,0205,0405,060
    ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggctggtcggtcggccttcccggctcgcgtcttcaccaggacgttgaaataggcggaggtaggtcagataattaacaacggcccttcgatctcattcatcaagcggtcaattatcaaacgcgttgcaacaacggtaacgatgtccgtagcaccacagtgcgagcagcaaaccataccgaAclIFspIBsrDIAmp (bla)5,0805,1005,1205,1405,1605,1805,2005,2205,240
    tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaaagtaagtcgaggccaagggttgctagttccgctcaatgtactagggggtacaacacgttttttcgccaatcgaggaagccaggaggctagcaacagtcttcattcaaccggcgtcacaatagtgagtaccaataccgtcgtgacgtattaagagaatgacagtacggtaggcattAmp (bla)5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttctacgaaaagacactgaccactcatgagttggttcagtaagactcttatcacatacgccgctggctcaacgagaacgggccgcagttatgccctattatggcgcggtgtatcgtcttgaaattttcacgagtagtaaccttttgcaagaagccccgcttttgagagttcctagaaAclIBcgIScaIXmnIAmp (bla)5,4405,4605,4805,5005,5205,5405,5605,5805,600
    accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattggcgacaactctaggtcaagctacattgggtgagcacgtgggttgactagaagtcgtagaaaatgaaagtggtcgcaaagacccactcgtttttgtccttccgttttacggcgttttttcccttattcccgctgtgcctttacaacttatgagtatgagaaggaaaaagttataSspIAmp (bla)bla promoter5,6205,6405,6605,6805,7005,7205,7405,760
    tattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgaataacttcgtaaatagtcccaataacagagtactcgcctatgtataaacttacataaatctttttatttgtttatccccaaggcgcgtgtaaaggggcttttcacggtggactgcagctgcctagccctctagagggctaggggataccacgtgagagtcatgttagacgagactBspHISgrDIbla promoter5,7805,8005,8205,8405,8605,8805,9005,9205,940
    tgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgtacgggccagatatacgcgttgacaacggcgtatcaattcggtcatagacgagggacgaacacacaacctccagcgactcatcacgcgctcgttttaaattcgatgttgttccgttccgaactggctgttaacgtacttcttagacgaatcccaatccgcaaaacgcgacgaagcatgcccggtctatatgcgcaactgtMfeIMluIBsiWIBpu10I5,9605,9806,0006,0206,0406,0606,0806,1006,120
    ttgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttaaactaataactgatcaataattatcattagttaatgccccagtaatcaagtatcgggtatatacctcaaggcgcaatgtattgaatSpeICMV promoter (with TetOs)CMV6,1406,1606,1806,200
    • Plasmid
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6211 bp45005000550050010001500CMV promoter (with TetOs)CMV670-12061243-16981207-1242667-669FrtF (DNA)M13-48REV Genewiz (DNA)pUCoriR (DNA)EKo272.1 (DNA)EK_CMVFb (DNA),EK_CMVFc (DNA),EK_CMVFd (DNA),SnaBI,NcoIA,TATA box,EK_CMVFe (DNA),CMV-Forward Genewiz (DNA),CspCITetO,TetR binding sites,TetO,CMVF1 (DNA),Eco53kI,SacI,BsaBI,BsaBI,+3Kozak,start codon,ToFlpF (DNA),EKo283.2 (DNA),PmeI,AflII,HindIIIBamHIPfoI,BcgI,AhdIUtroUpTspMI,XmaI,SmaIEWo211 (DNA)N (NCOA3) Activation Domain,BsgI,BlpI,BstXIEW_NZF_R (DNA),PasI,PflMINZF Domain,Z (ZNF473) Activation Domain,EcoRI,BmgBI,PasIEcoNIPpuMIBspHIAhdIBsaI,BsrDIFspIAclI,BsrDIAmp (bla)ScaI,BcgIAclIXmnIbla promoter,SspI,BspHI,SgrDIFlpInF1 (DNA),FlpInF (DNA),EKo279.1 (DNA),EK_CMVFa (DNA),MfeI,Bpu10I,BsiWI,MluI,+1

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    6211