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pET-31b JfyP (To be transformed)
  • Sequence Map
    Editing disabled on read-only sequence.
    ggagctgacgcaggaggtacgcgcggtcgccaacgaagcggccttcgctttcaccgtcagcttcgagtatcagggccgcaagaccgtagttgcgcccatcgatATGACCAGCGAACAATACTCAGTCAAGCTTACACCAGACTTCGACAACCCAAAATGGATTGGACGACACAAGcctcgactgcgtcctccatgcgcgccagcggttgcttcgccggaagcgaaagtggcagtcgaagctcatagtcccggcgttctggcatcaacgcgggtagctaTACTGGTCGCTTGTTATGAGTCAGTTCGAATGTGGTCTGAAGCTGTTGGGTTTTACCTAACCTGCTGTGTTCClaIBspDIHindIIIJfyP20406080100120140160
    CACATGTTTAATTTTCTTGATGTCAACCACAATGGAAGGATCTCTCTTGACGAGATGGTCTACAAGGCGTCCGATATTGTTATAAACAATCTTGGAGCAACACCTGAACAAGCCAAACGTCACAAAGATGCTGTAGAAGCCTTCTTCGGAGGAGCTGGAATGAAATATGGTGTAGGTGTACAAATTAAAAGAACTACAGTTGGTGTTACCTTCCTAGAGAGAACTGCTCTACCAGATGTTCCGCAGGCTATAACAATATTTGTTAGAACCTCGTTGTGGACTTGTTCGGTTTGCAGTGTTTCTACGACATCTTCGGAAGAAGCCTCCTCGACCTTACTTTATACCACATCAccIPciIPsiIBseRIHincIIJfyP180200220240260280300320340
    AAACTGAATGGCCTGAATACATCGAAGGATGGAAAAGACTGGCTTCCGAGGAATTGAAAAGGTATTCAAAAAACCAAATCACACTTATTCGTTTATGGGGTGATGCATTGTTCGATATCATTGACAAAGACCAAAATGGAGCTATTTCACTGGATGAATGGAAAGCATACACCAATTTGACTTACCGGACTTATGTAGCTTCCTACCTTTTCTGACCGAAGGCTCCTTAACTTTTCCATAAGTTTTTTGGTTTAGTGTGAATAAGCAAATACCCCACTACGTAACAAGCTATAGTAACTGTTTCTGGTTTTACCTCGATAAAGTGACCTACTTACCTTTCGTATGTGGTTNsiIEcoRVJfyP360380400420440460480500520
    ATCTGATGGCATCATCCAATCGTCAGAAGATTGCGAGGAAACATTCAGAGTGTGCGATATTGATGAAAGTGGACAGCTCGATGTTGATGAGATGACAAGACAACATTTAGGATTTTGGTACACCATGGATCCTGCTTGCGAAAAGCTCTACGGTGGAGCTGTCCCCTAAcagatgTAGACTACCGTAGTAGGTTAGCAGTCTTCTAACGCTCCTTTGTAAGTCTCACACGCTATAACTACTTTCACCTGTCGAGCTACAACTACTCTACTGTTCTGTTGTAAATCCTAAAACCATGTGGTACCTAGGACGAACGCTTTTCGAGATGCCACCTCGACAGGGGATTgtctacNcoIStyIBamHIJfyP540560580600620640660680700
    ctgcaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgcgccctggacgtggtggtggtgactctaggccgacgattgtttcgggctttccttcgactcaaccgacgacggtggcgactcgttattgatcgtattggggaaccccggagatttgcccagaactccccaaaaaacgactttcctccttgatataggcctaaccgcttaccctgcgcgggacBlpIStyIAlwNIBspEI720740760780800820840860
    tagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctatcgccgcgtaattcgcgccgcccacaccaccaatgcgcgtcgcactggcgatgtgaacggtcgcgggatcgcgggcgaggaaagcgaaagaagggaaggaaagagcggtgcaagcggccgaaaggggcagttcgagatttagcccccgagggaaatcccaaggctaaatcacga8809009209409609801,0001,0201,040
    ttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataatgccgtggagctggggttttttgaactaatcccactaccaagtgcatcacccggtagcgggactatctgccaaaaagcgggaaactgcaacctcaggtgcaagaaattatcacctgagaacaaggtttgaccttgttgtgagttgggatagagccagataagaaaactaaataAloIPsiIBsaAIBsaXIDraIII1,0601,0801,1001,1201,1401,1601,1801,2001,220
    aagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctttccctaaaacggctaaagccggataaccaattttttactcgactaaattgtttttaaattgcgcttaaaattgttttataattgcaaatgttaaagtccaccgtgaaaagcccctttacacgcgccttggggataaacaaataaaaagatttatgtaagtttatacataggcgaSspIAmpR_promoter1,2401,2601,2801,3001,3201,3401,3601,3801,400
    catgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtactctgttattgggactatttacgaagttattataactttttccttctcatactcataagttgtaaaggcacagcgggaataagggaaaaaacgccgtaaaacggaaggacaaaaacgagtgggtctttgcgaccactttcattttctacgacttctagtcaacccacgtgctAcuISspIBssSIBssSαIAmpicillinAm...r1,4201,4401,4601,4801,5001,5201,5401,560
    gtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactcacccaatgtagcttgacctagagttgtcgccattctaggaactctcaaaagcggggcttcttgcaaaaggttactactcgtgaaaatttcaagacgatacaccgcgccataatagggcataactgcggcccgttctcgttgagccagcggcgtatgtgataagagtcttactgaXmnIAmpicillin1,5801,6001,6201,6401,6601,6801,7001,7201,740
    tggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaacaccaactcatgagtggtcagtgtcttttcgtagaatgcctaccgtactgtcattctcttaatacgtcacgacggtattggtactcactattgtgacgccggttgaatgaagactgttgctagcctcctggcttcctcgattggcgaaaaaacgtgttgtaccccctagtacattgPvuIScaITatIAmpicillin1,7601,7801,8001,8201,8401,8601,8801,9001,920
    tcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaagcggaactagcaacccttggcctcgacttacttcggtatggtttgctgctcgcactgtggtgctacggacgtcgttaccgttgttgcaacgcgtttgataattgaccgcttgatgaatgagatcgaagggccgttgttaattatctgacctacctccgcctatttcaacgtcctFspIPstINmeAIIIAmpicillin1,9401,9601,9802,0002,0202,0402,0602,0802,100
    ccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagaggtgaagacgcgagccgggaaggccgaccgaccaaataacgactatttagacctcggccactcgcacccagagcgccatagtaacgtcgtgaccccggtctaccattcgggagggcatagcatcaatagatgtgctgcccctcagtccgttgatacctacttgctttatctgtctAhdIBglIBsaIAmpicillin2,1202,1402,1602,1802,2002,2202,2402,260
    tcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcagcgactctatccacggagtgactaattcgtaaccattgacagtctggttcaaatgagtatatatgaaatctaactaaattttgaagtaaaaattaaattttcctagatccacttctaggaaaaactattagagtactggttttagggaattgcactcaaaagcaaggtgactcgAmpicillin2,2802,3002,3202,3402,3602,3802,4002,4202,440
    gtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatcagtctggggcatcttttctagtttcctagaagaactctaggaaaaaaagacgcgcattagacgacgaacgtttgtttttttggtggcgatggtcgccaccaaacaaacggcctagttctcgatggttgagaaaaaggcttccattgaccgaagtcgtctcgcgtctatggtttaAcuI2,4602,4802,5002,5202,5402,5602,5802,6002,620
    actgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggtgacaggaagatcacatcggcatcaatccggtggtgaagttcttgagacatcgtggcggatgtatggagcgagacgattaggacaatggtcgacggtcaccgctattcagcacagaatggcccaacctgagttctgctatcaatggcctattccgcgtcgccagcccgacttgcc2,6402,6602,6802,7002,7202,7402,7602,7802,800
    ggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcccccaagcacgtgtgtcgggtcgaacctcgcttgctggatgtggcttgactctatggatgtcgcactcgatactctttcgcggtgcgaagggcttccctctttccgcctgtccataggccattcgccgtcccagccttgtcctctcgcgtgctccctcgaaggtccccctttgcgBssSIBssSαI2,8202,8402,8602,8802,9002,9202,9402,960
    ctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgatgaccatagaaatatcaggacagcccaaagcggtggagactgaactcgcagctaaaaacactacgagcagtccccccgcctcggatacctttttgcggtcgttgcgccggaaaaatgccaaggaccggaaaacgaccggaaaacgagtgtacaagaaaggacgcaataggggactaPciI2,9803,0003,0203,0403,0603,0803,1003,1203,140
    tctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatagacacctattggcataatggcggaaactcactcgactatggcgagcggcgtcggcttgctggctcgcgtcgctcagtcactcgctccttcgccttctcgcggactacgccataaaagaggaatgcgtagacacgccataaagtgtggcgtatataccacgtgagagtcatgttaSapITatIBspQI3,1603,1803,2003,2203,2403,2603,2803,3003,320
    ctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagagacgagactacggcgtatcaattcggtcatatgtgaggcgatagcgatgcactgacccagtaccgacgcggggctgtgggcggttgtgggcgactgcgcgggactgcccgaacagacgagggccgtaggcgaatgtctgttcgacactggcagaggccctcgacgtacacagtctAccIPfoIBsaAIBsmBIEsp3IPflFIBstZ17ITth111IpGEX_3_primer3,3403,3603,3803,4003,4203,4403,4603,4803,500
    ggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcccaaaagtggcagtagtggctttgcgcgctccgtcgacgccatttcgagtagtcgcaccagcacttcgctaagtgtctacagacggacaagtaggcgcaggtcgagcaactcaaagaggtcttcgcaattacagaccgaagactatttcgcccggtacaattcccgccaaaaaagXmnIROP3,5203,5403,5603,5803,6003,6203,6403,660
    ctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaagacaaaccagtgactacggaggcacattccccctaaagacaagtacccccattactatggctactttgctctctcctacgagtgctatgcccaatgactactacttgtacgggccaatgaccttgcaacactcccatttgttgaccgccatacctacgccgccctggtctcttttROP3,6803,7003,7203,7403,7603,7803,8003,8203,840
    atcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgtagtgagtcccagttacggtcgcgaagcaattatgtctacatccacaaggtgtcccatcggtcgtcgtaggacgctacgtctaggccttgtattaccacgtcccgcgactgaaggcgcaaaggtctgaaatgctttgtgcctttggcttctggtaagtacaacaacgagtccagcBspEIBpu10I3,8603,8803,9003,9203,9403,9603,9804,0004,020
    cagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttgtctgcaaaacgtcgtcgtcagcgaagtgcaagcgagcgcatagccactaagtaagacgattggtcattccgttggggcggtcggatcggcccaggagttgctgtcctcgtgctagtacgcgtgggcaccccggcggtacggccgctattaccggacgaagagcggctttgcaaaBglIFspIFspAIPpuMItet (576 - 851)4,0404,0604,0804,1004,1204,1404,1604,1804,200
    ggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcgccaccgccctggtcactgcttccgaactcgctcccgcacgttctaaggcttatggcgttcgctgtccggctagtagcagcgcgaggtcgctttcgccaggagcggcttttactgggtctcgcgacggccgtggacaggatgctcaacgtactatttcttctgtcagtattcacgctet (576 - 851)4,2204,2404,2604,2804,3004,3204,3404,360
    gcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcgcgctgctatcagtacggggcgcgggtggccttcctcgactgacccaacttccgagagttcccgtagccagctctagggccacggattactcactcgattgaatgtaattaacgcaacgcgagtgacgggcgaaaggtcagccctttggacagcacggtcgacgtaattacttagcPshAItet (576 - 851)lacI4,3804,4004,4204,4404,4604,4804,5004,5204,540
    gccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacgcggttgcgcgcccctctccgccaaacgcataacccgcggtcccaccaaaaagaaaagtggtcactctgcccgttgtcgactaacgggaagtggcggaccgggactctctcaacgtcgttcgccaggtgcgaccaaacggggtcgtccgcttttaggacaaactaccaccaattgcHpaIBsaXIBsmBIEsp3IHincIIlacI4,5604,5804,6004,6204,6404,6604,6804,7004,720
    gcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgcgccctatattgtactcgacagaagccatagcagcatagggtgatggctctataggcgtggttgcgcgtcgggcctgagccattaccgcgcgtaacgcgggtcgcggtagactagcaaccgttggtcgtagcgtcacccttgctacgggagtaagtcgtaaacgtaccaaacaacEcoRVBssHIIlacI4,7404,7604,7804,8004,8204,8404,8604,8804,900
    aaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtttttggcctgtaccgtgaggtcagcggaagggcaaggcgatagccgacttaaactaacgctcactctataaatacggtcggtcggtctgcgtctgcgcggctctgtcttgaattacccgggcgattgtcgcgctaaacgaccactgggttacgctggtctacgaggtgcgggtcaApaIBstEIIPspOMINmeAIIIlacI4,9204,9404,9604,9805,0005,0205,0405,060
    cgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccggcgcatggcagaagtaccctcttttattatgacaactacccacagaccagtctctgtagttctttattgcggccttgtaatcacgtccgtcgaaggtgtcgttaccgtaggaccagtaggtcgcctatcaattactagtcgggtgactgcgcaacgcgctcttctaacacgtggcBclIMluIlacI5,0805,1005,1205,1405,1605,1805,2005,2205,240
    ccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacggcgaaatgtccgaagctgcggcgaagcaagatggtagctgtggtggtgcgaccgtgggtcaactagccgcgctctaaattagcggcgctgttaaacgctgccgcgcacgtcccggtctgacctccaccgttgcggttagtcgttgctgacaaacgggcggtcaacaacacggtglacI5,2605,2805,3005,3205,3405,3605,3805,4005,420
    gcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactccgccaacccttacattaagtcgaggcggtagcggcgaaggtgaaaaagggcgcaaaagcgtctttgcaccgaccggaccaagtggtgcgccctttgccagactattctctgtggccgtatgagacgctgtagcatattgcaatgaccaaagtgtaagtggtgggacttaactgagBstAPIlacI5,4405,4605,4805,5005,5205,5405,5605,5805,600
    tcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagaaggcccgcgatagtacggtatggcgctttccaaaacgcggtaagctaccacaggccctagagctgcgagagggaatacgctgaggacgtaatccttcgtcgggtcatcatccaactccggcaactcgtggcggcggcgttccttaccacgtacgttcctctaccgcgggttgPfoISphIEcoNIPflMItet (300 - 563)5,6205,6405,6605,6805,7005,7205,7405,760
    agtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatcttcagggggccggtgccccggacggtggtatgggtgcggctttgttcgcgagtactcgggcttcaccgctcgggctagaaggggtagccactacagccgctatatccgcggtcgttggcgtggacaccgcggccactacggccggtgctacgcaggccgcatctcctagctctagaBglIISgrAItet (300 - 563)pBRrevB...primer5,7805,8005,8205,8405,8605,8805,9005,9205,940
    cgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatgcataccccagaacacatcaccgccgtggtacagcgctttgtggctgcgctcaatgccggcgatctggacggctagggcgctttaattatgctgagtgatatccccttaacactcgcctattgttaaggggagatctttattaaaacaaattgaaattcttcctctatatgtatacgtatggggtcttgtgtagtggcggcaccatgtcgcgaaacaccgacgcgagttacggccgctagacctgcAleIBarINdeINsiIXbaI5,9605,9806,0006,0206,0406,0606,0806,1006,120
    gcatcgtcgcgctgtttgccgatgacgccacggtggaagaccccgtgggttccgagcccaggtccggtacggctgcgattcgtgagttttacgccaactcgctcaaactgcctttggcggtcgtagcagcgcgacaaacggctactgcggtgccaccttctggggcacccaaggctcgggtccaggccatgccgacgctaagcactcaaaatgcggttgagcgagtttgacggaaaccgcca6,1406,1606,1806,2006,2206,240
    • Plasmid
    • Assembly history
    • Relevant Items
    • Metadata
    • Description
    • Linear Map
    Editing disabled on read-only sequence.
    6246 bp45005000550050010001500lacIAmpicillinpBRrevBam_primerRev Seq Primer T7 terminator (DNA)

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    6246